混合碱胁迫对碱茅和紫花苜蓿种子萌发的影响
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摘要
采用室内种子萌发试验方法,以碱茅(Puccinellia distans),紫花苜蓿(Medicago sativa L.)两种牧草种子为研究对象,以NaHCO_3和Na_2CO_3为基础盐分,将NaHCO_3和Na_2CO_3按照1:0、2:1、1:1、1:2、1:1的比例配成相同浓度的复合溶液,研究不同混合盐分胁迫对碱茅和紫花苜蓿种子发芽率、发芽势、发芽指数、芽长和芽重的影响。结果表明:不同比例的混合盐分胁迫对碱茅和苜蓿种子的发芽率、发芽势、发芽指数和芽长均有明显地抑制作用。但是,不同盐分组合处理间对碱茅和紫花苜蓿的芽重的影响比单一盐分要小。单一盐分比较,NaHCO_3的抑制作用更强。NaHCO_3和Na_2CO_3的比例为1:1时,对两种种子的发芽抑制作用最强。两种牧草比较,Na_2CO_3和NaHCO_3混合盐分胁迫对碱茅种子萌发的抑制作用较弱,对紫花苜蓿种子萌发的抑制作用较强。
The effects of two saline-alkaloid(NaHCO_3,Na_2CO_3) and their mixed solutions(1:0、2:1、1:1、1:2、1:1) on Puccinellia distans and Medicago sativa L.seed germination rate,germination potential,germinationindex,bud length and bud weight were studied by indoor experiments.The results showed that the germinationrate,germination potential,germination index and bud length were obviously inhibited by the mixed saline-alkaloid solutions,but the effect of mixed saline-alkaloid solutions on bud weight was smaller than that ofsingle saline-alkaloid treatment.The inhibiting effect of NaHCO_3 was higher than that of Na_2CO_3,and when themixed ratio of the two saline-alkaloid was 1:1,the inhibiting effect was the highest.The mixed saline-alkaloidstress had poor inhibition on Puccinellia distans seed germination,but strong inhibition on Medicago sativa L.seed germination.
The effects of two saline-alkaloid(NaHCO_3,Na_2CO_3) and their mixed solutions(1:0、2:1、1:1、1:2、1:1) on Puccinellia distans and Medicago sativa L.seed germination rate,germination potential,germinationindex,bud length and bud weight were studied by indoor experiments.The results showed that the germinationrate,germination potential,germination index and bud length were obviously inhibited by the mixed saline-alkaloid solutions,but the effect of mixed saline-alkaloid solutions on bud weight was smaller than that ofsingle saline-alkaloid treatment.The inhibiting effect of NaHCO_3 was higher than that of Na_2CO_3,and when themixed ratio of the two saline-alkaloid was 1:1,the inhibiting effect was the highest.The mixed saline-alkaloidstress had poor inhibition on Puccinellia distans seed germination,but strong inhibition on Medicago sativa L.seed germination.
引文
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[26]纪荣花,于磊,鲁为华,等.盐胁迫对芨芨草种子萌发的影响[J].草业科学,2011,28(2):245-250.
[27]高战武,蔺吉祥,邵帅,等.复合盐碱胁迫对燕麦种子发芽的影响[J].草业科学,2014,31(3):451-456.
[28]尚培培,李丰先,周宇飞,等.混合碱(Na HCO3和Na2CO3)胁迫对高粱幼苗渗透调节和离子平衡的影响[J].生态学杂志,2015,34(7):1924-1929.
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[2]丁雪梅,何汉琼,张英,等.野大麦种子萌发条件的研究[J].东北农业大学学报,2010,41(6):11-16.
[3]张颖超,贾玉山,任永霞.钠盐胁迫对白花草木樨种子发芽的影响[J].草业科学,2013,30(12):2005-2010.
[4]景艳霞,袁庆华.Na Cl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J].草业学报,2011,20(2):134-139.
[5]廉佳杰,张凤,毛培胜,等.碱茅种子发芽检测标准方法的研究[J].草地学报,2010,18(2):247-251.
[6]Liu J,Guo W Q,Shi D C,et al.Seed germination,seedling survival,and physiological response of sunflowers under saline and alkaline conditions[J].Photosynthetica,2010,48:278-286
[7]张海南,周青平,颜红波,等.盐胁迫对5种碱茅材料种子萌发的影响[J].草业科学,2013,30(11):1767-1770.
[8]卢艳敏.不同盐胁迫对高羊茅种子萌发的影响[J].草业科学,2012,29(7):1088-1093.
[9]韩润燕,周志红,冯进萍.Na Cl胁迫对草木樨种子萌发及幼苗生长的影响[J].干旱地区农业研究,2014,32(5):79-83.
[10]张舟,邬忠康,陈志成,等.盐胁迫对4种豇豆种子萌发的影响[J].种子,2014,33(3):19-23.
[11]沈振荣,杨万仁,徐秀梅.不同盐分胁迫对苜蓿种子萌发的影响[J].种子,2006,25(4):34-37.
[12]禹婷.盐胁迫对6种禾本科牧草种子萌发及幼苗生长的影响[J].种子,2014,33(6):89-90.
[13]古丽内尔·亚森,杨瑞瑞,曾幼玲.混合盐碱胁迫对灰绿藜种子萌发的影响[J].生态学杂志,2014,33(1):76-82.
[14]景艳霞,袁庆华.不同钠盐胁迫对苜蓿种子萌发的影响[J].种子,2010,29(2):69-72.
[15]郭立泉,陈建欣,崔景军,等.盐、碱胁迫下星星草有机酸代谢调节的比较研究[J].东北师范大学学报:自然科学版,2009,41(4):123-128.
[16]吴成龙,周春霖,尹金来,等.碱胁迫对不同品种菊芋幼苗生物量分配和可溶性渗透物质含量的影响[J].中国农业科学,2008,41(3):901-909.
[17]郭彦,杨洪汉,赵家斌.混合盐碱对大豆种子萌发的影响[J].种子,2008,27(12):92-94.
[18]郑庆钟,李发明,朱淑娟,等.不同处理对沙生针茅种子萌发的影响[J].生态学杂志,2016,35(1):63-71.
[19]杨持.生态学实验与实习(第二版)[M].北京:高等教育出版社,2008:48.
[20]Li R L,Shi F C,Fukuda K,et al.Interactive effects of salt and alkali stresses on seed germination,germination recovery,and seedling growth of a halophyte Spartina alterniflora(Poaceae)[J].South African Journal of Botany,2010b,76:380-387.
[21]王永娟,周妍,徐明,等.盐胁迫对大豆种子萌发及矿质元素变化的影响[J].生态学杂志,2015,34(6):1565-1571.
[22]Yang J Y,Zheng W,Tian Y,et al.Effects of various mixed saltalkaline stresses on growth,photosynthesis,and photosynthetic pigment concentration of Medicago ruthenica seedlings[J].Photosynthetica,2011,49:275-284
[23]樊瑞苹,周琴,周波,等.盐胁迫对高羊茅生长及抗氧化系统的影响[J].草业学报,2012,21(1):112-117.
[24]唐素英.芨芨草生物学特性和抗盐性的研究[D].石河子:石河子大学,2002.
[25]张永峰,殷波.混合盐胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J].草业学报,2009,18(1):46-50.
[26]纪荣花,于磊,鲁为华,等.盐胁迫对芨芨草种子萌发的影响[J].草业科学,2011,28(2):245-250.
[27]高战武,蔺吉祥,邵帅,等.复合盐碱胁迫对燕麦种子发芽的影响[J].草业科学,2014,31(3):451-456.
[28]尚培培,李丰先,周宇飞,等.混合碱(Na HCO3和Na2CO3)胁迫对高粱幼苗渗透调节和离子平衡的影响[J].生态学杂志,2015,34(7):1924-1929.
[29]王宁,曹敏建,王君,等.Na Cl和Na2CO3+Na HCO3对玉米种子萌发及幼苗生长的影响差异研究[J].作物杂志,2009(4):52-56.