基于隶属函数法的甜高粱孕穗期耐盐性综合评价
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摘要
【目的】研究甜高粱孕穗期对盐胁迫的生理响应,探明不同甜高粱品系(种)孕穗期耐盐性,为甜高粱在盐渍化土地的种植提供理论依据。【方法】以5个品系(种)甜高粱(F373、A47、A60、A39和大力士)为试验材料,设盐胁迫(含盐量5.76 g/kg)和对照(含盐量1.85 g/kg)2个处理,测定孕穗期甜高粱大田生理指标及相关酶活性,利用主成分分析法和隶属函数法对孕穗期的耐盐性进行综合评价。【结果】与对照相比,盐胁迫下各品系(种)甜高粱的株高和叶绿素相对含量(SPAD值)降低,冠层温度、细胞膜透性、脯氨酸和丙二醛(MDA)含量升高。不同品系(种)甜高粱的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性变化趋势一致,除A39外其他品系(种)甜高粱的过氧化氢酶(CAT)活性在盐胁迫下显著升高(P<0.05,下同)。盐胁迫下5个甜高粱品系(种)耐盐性排序为:A47>F373>A60>A39>大力士。相关性分析结果表明,不同品系(种)甜高粱的株高和SPAD值与综合评价D值分别呈极显著(P<0.01)和显著正相关,相关系数分别为0.95和0.83。【结论】株高和SPAD值可作为甜高粱孕穗期耐盐品系(种)筛选的主要参考指标。
【Objective】The present study aimed to investigate the physiological response of sweet sorghum to salt stress during the booting stage,determine salt tolerance at booting stage in different sweet sorghum lines(varieties)and provide theoretical basis for the cultivation of sweet sorghum in saline land.【Method】Five sweet sorghum lines(varieties),namely F373,A47,A60,A39 and Dalishi were selected as materials,and salt stress(salt content 5.76 g/kg)and control(salt content 1.85 g/kg)were set to determine field physiological parameters and related enzyme activities at booting stage of sweet sorghum. Principal component analysis and membership function method were used to comprehensively evaluate salt tolerance of sweet sorghum at booting stage.【Result】Compared with the control,the plant height and relative chlorophyll content(SPAD value)of the each sweet sorghum lines(varieties)under the salt stress decreased,and canopy temperature,cell membrane permeability,proline content and malonaldehyde(MDA)content increased. The variation trends of superoxide dismutase(SOD)activity and peroxidase(POD)activity in different lines(varieties)of sweet sorghum were consistent,and the catalase(CAT)activities of sweet sorghum lines(varieties)except A39 increased significantly under salt stress(P<0.05,the same below). The order of salinity tolerance of five lines(varieties)of sweet sorghum was:A47>F373>A60>A39>Dalishi. Correlation analysis results showed that the positive correlation between plant height and SPAD value was extremely significant(P<0.01),and that between plant height and comprehensive evaluation D value was significant,and the correlation coefficients were 0.95 and 0.83 respectively.【Conclusion】Plant height and SPAD value can be used as reliable reference parameters for selecting salt-tolerant sweet sorghum lines(varieties)at the booting stage.
【Objective】The present study aimed to investigate the physiological response of sweet sorghum to salt stress during the booting stage,determine salt tolerance at booting stage in different sweet sorghum lines(varieties)and provide theoretical basis for the cultivation of sweet sorghum in saline land.【Method】Five sweet sorghum lines(varieties),namely F373,A47,A60,A39 and Dalishi were selected as materials,and salt stress(salt content 5.76 g/kg)and control(salt content 1.85 g/kg)were set to determine field physiological parameters and related enzyme activities at booting stage of sweet sorghum. Principal component analysis and membership function method were used to comprehensively evaluate salt tolerance of sweet sorghum at booting stage.【Result】Compared with the control,the plant height and relative chlorophyll content(SPAD value)of the each sweet sorghum lines(varieties)under the salt stress decreased,and canopy temperature,cell membrane permeability,proline content and malonaldehyde(MDA)content increased. The variation trends of superoxide dismutase(SOD)activity and peroxidase(POD)activity in different lines(varieties)of sweet sorghum were consistent,and the catalase(CAT)activities of sweet sorghum lines(varieties)except A39 increased significantly under salt stress(P<0.05,the same below). The order of salinity tolerance of five lines(varieties)of sweet sorghum was:A47>F373>A60>A39>Dalishi. Correlation analysis results showed that the positive correlation between plant height and SPAD value was extremely significant(P<0.01),and that between plant height and comprehensive evaluation D value was significant,and the correlation coefficients were 0.95 and 0.83 respectively.【Conclusion】Plant height and SPAD value can be used as reliable reference parameters for selecting salt-tolerant sweet sorghum lines(varieties)at the booting stage.
引文
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姜慧,黄健,张云鹤,胡瑞芳,李玥莹.2012.盐胁迫对甜高粱幼苗抗氧化酶活性的影响[J].沈阳师范大学学报(自然科学版),30(2):289-292.[Jiang H,Huang J,Zhang YH,Hu R F,Li Y Y.2012.Effect of salt stress on antioxidative enzymes in sweet sorghum seedlings[J].Journal of Shenyang Normal University(Natural Science),30(2):289-292.]
蓝颖,许建新,冯志坚.2010.盐胁迫对毛黄肉楠生理特性的影响[J].中国园艺文摘,(7):34-35.[Lan Y,Xu J X,Feng Z J.2010.Effect of salt stress on the physiological characteristics of Actinodaphne pilosa[J].Chinese Horticulture Abstracts,(7):34-35.]
李春宏,郭文琦,张培通,殷剑美,韩晓勇,王立.2016.土壤盐胁迫降低后甜高粱的补偿生长和盐离子分布特征[J].中国生态农业学报,24(5):637-642.[Li C H,Guo W Q,Zhang P T,Yin J M,Han X Y,Wang L.2016.The compensation growth and salt ion distribution in sweet sorghum(Sorghum bicolor L.Moench)after salt stress reduction[J].Chinese Journal of Eco-Agriculture,24(5):637-642.]
刘芳,付艳,高树仁,王振华.2007.玉米幼苗的盐胁迫反应及玉米耐盐性的鉴定[J].黑龙江八一农垦大学学报,19(6):22-26.[Liu F,Fu Y,Gao S R,Wang Z H.2007.Response under salt-stress of maize in seedling stage and appraisal of salt tolerance of maize[J].Journal of Heilongjiang August First Land Reclamation University,19(6):22-26.]
刘茂松,鲁小珍,王汉杰,邓存恒,任荣荣,唐荣南.2001.宁夏平罗西大滩人类有序活动的环境效应及发展对策[J].南京林业大学学报(自然科学版),25(3):83-88.[Liu MS,Lu X Z,Wang H J,Deng C H,Ren R R,Tang R N.2001.The environmental effectiveness and countermeasure of human activity in Xidatan of Pingluo in Ningxia autonomous region[J].Journal of Nanjing Forestry University(Natural Sciences Edition),25(3):83-88.]
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