玉米杂交种DK516亲本的耐盐性研究
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摘要
利用不同浓度的NaCl、Na_2SO_4,对玉米杂交种DK516亲本的萌发期和苗期2个生长阶段进行胁迫处理,以对其进行耐盐性鉴定。结果表明:除发芽率不受影响外,发芽势、胚芽长度、胚芽鲜重、胚芽干重、胚芽含水量、发芽指数、活力指数、出苗率、株高和生物量等指标均随盐浓度的升高呈整体显著下降趋势,综合表现为DK516♀耐盐性优于DK516♂;DK516亲本的地上部分对盐胁迫较地下部分敏感,DK516♂根系较DK516♀发达,初步推断DK516♂抗倒伏能力相对较好,但此推断有待大田试验进一步验证。
Different concentration gradients of NaCl and Na_2SO_4 were applied to stress treatment of the maize inbred lines DK516 parent at germination stage and growth stage to identify the salt resistance of the parent of DK516. The results showed that,except for the germination rate,other indexes including germination potential,germ length,germ fresh weight,germ dry weight,germ water content,germination index,vitality index,seedling rate,plant height and biomass were all significantly decreased with the increase of salt concentration. The comprehensive performance is that the salt tolerance of DK516♀ is better than that of DK516♂. The above-ground part of the parent of DK516 is more sensitive to salt stress than the underground part. The root system of DK516♂ is more developed than that of DK516♀,and it is preliminarily inferred that DK516♂ is relatively good at resistance lodging. However,this inference is subject to further verification by field test.
Different concentration gradients of NaCl and Na_2SO_4 were applied to stress treatment of the maize inbred lines DK516 parent at germination stage and growth stage to identify the salt resistance of the parent of DK516. The results showed that,except for the germination rate,other indexes including germination potential,germ length,germ fresh weight,germ dry weight,germ water content,germination index,vitality index,seedling rate,plant height and biomass were all significantly decreased with the increase of salt concentration. The comprehensive performance is that the salt tolerance of DK516♀ is better than that of DK516♂. The above-ground part of the parent of DK516 is more sensitive to salt stress than the underground part. The root system of DK516♂ is more developed than that of DK516♀,and it is preliminarily inferred that DK516♂ is relatively good at resistance lodging. However,this inference is subject to further verification by field test.
引文
[1]魏国强,朱祝军,方学智. Na Cl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响[J].中国农业科学,2004,37(11):1754-1759.
[2] Takehisa H,Shimodate T,Fukuta Y. Identification of quantitative trait loci for plant growth of rice in paddy field flooded with salt water[J]. Field Crops Res,2004,89:85-95.
[3]段显德,周波,杨国会.玉米耐盐性研究[J].通化师范学院学报,2001,22(2):67-69.
[4]王广印,周秀梅,张建伟.不同黄瓜材料种子萌发期的耐盐性研究[J].植物遗传资源学报,2004,5(3):299-303.
[5]李雅华,崔平,王艳玲.甜菜种质发芽期耐盐性及鉴定方法研究[J].中国糖料,2000(3):5-8.
[6]袁鹰,刘德璞,李光弟.玉米常规育种中存在的问题的生物技术解决方案[J].分子植物育种,2005,3(1):125-139.
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[9]徐艳霞.利用TOPSIS法综合评价芽苗期青贮玉米种质耐盐性[J].杂粮作物,2010,30(2):101-104.
[10]贾亚雄,李向林,袁庆华,等.披碱草属野生种质资源苗期耐盐性评价及相关生理机制研究[J].中国农业科学,2008,41(10):2999-3007.
[11]程玉静,孙权星,彭长俊,等. 2种不同基因型玉米苗期盐胁迫反应及耐盐性研究[J].上海农业学报,2012,28(2):53-58.
[12]张海艳,赵延明. Na Cl胁迫对糯玉米种子发芽和幼苗生长的影响[J].植物生理学通讯,2010,46(3):291-293.
[13]彭云玲,李伟丽,王泽坤,等. Na Cl胁迫对玉米耐盐系与盐敏感系萌发和幼苗生长的影响[J].草业学报,2012,21(4):62-71.
[14]王丽燕,赵可夫.玉米幼苗对盐胁迫的生理响应[J].作物学报,2005,31(2):264-266.
[15]孙巧玲.不同盐分在不同浓度条件下对玉米幼苗基础生理效应的研究[J].山东师范大学学报(自然科学版),2000,15(1):77-82.
[16] Asif AK,Sajjad AR,Thomas M. Assessment of salinity tolerance based upon seedling root growth response functions in maize[J]. Euphytica,2003,131:81-89.
[17] Sairam RK,Srivastava GC,Agarwal S,et al. Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes[J]. Biologia Plantarum,2005,49(1):85-91.
[18]刘爱荣,张远兵,方园园,等.盐胁迫对金盏菊生长、抗氧化能力和盐胁迫蛋白的影响[J].草业学报,2011,20(6):52-59.
[19]景艳霞,袁庆华. Na Cl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J].草业学报,2011,20(2):134-139.
[20]过晓明,李强,王欣,等.盐胁迫对甘薯幼苗生理特性的影响[J].江苏农业科学,2011,39(3):107-109.
[21]陈树钢,丁彦芬.以电导法配合Logistic方程确定5种景天属植物耐旱性[J].江苏农业科学,2011,39(3):213-216.
[22]吴嘉雯,王庆亚.干旱胁迫对野生和栽培蒲公英抗性生理生化指标的影响[J].江苏农业学报,2010,26(2):264-271.
[2] Takehisa H,Shimodate T,Fukuta Y. Identification of quantitative trait loci for plant growth of rice in paddy field flooded with salt water[J]. Field Crops Res,2004,89:85-95.
[3]段显德,周波,杨国会.玉米耐盐性研究[J].通化师范学院学报,2001,22(2):67-69.
[4]王广印,周秀梅,张建伟.不同黄瓜材料种子萌发期的耐盐性研究[J].植物遗传资源学报,2004,5(3):299-303.
[5]李雅华,崔平,王艳玲.甜菜种质发芽期耐盐性及鉴定方法研究[J].中国糖料,2000(3):5-8.
[6]袁鹰,刘德璞,李光弟.玉米常规育种中存在的问题的生物技术解决方案[J].分子植物育种,2005,3(1):125-139.
[7] Munns R. Comparative physiology of salt and water stress[J]. Plant Cell and Environment,2002,25:239-250.
[8]姚正培,孟君,李冠.玉米自交系芽苗期耐盐性的鉴定与筛选[J].华北农学报,2007,22(5):27-30.
[9]徐艳霞.利用TOPSIS法综合评价芽苗期青贮玉米种质耐盐性[J].杂粮作物,2010,30(2):101-104.
[10]贾亚雄,李向林,袁庆华,等.披碱草属野生种质资源苗期耐盐性评价及相关生理机制研究[J].中国农业科学,2008,41(10):2999-3007.
[11]程玉静,孙权星,彭长俊,等. 2种不同基因型玉米苗期盐胁迫反应及耐盐性研究[J].上海农业学报,2012,28(2):53-58.
[12]张海艳,赵延明. Na Cl胁迫对糯玉米种子发芽和幼苗生长的影响[J].植物生理学通讯,2010,46(3):291-293.
[13]彭云玲,李伟丽,王泽坤,等. Na Cl胁迫对玉米耐盐系与盐敏感系萌发和幼苗生长的影响[J].草业学报,2012,21(4):62-71.
[14]王丽燕,赵可夫.玉米幼苗对盐胁迫的生理响应[J].作物学报,2005,31(2):264-266.
[15]孙巧玲.不同盐分在不同浓度条件下对玉米幼苗基础生理效应的研究[J].山东师范大学学报(自然科学版),2000,15(1):77-82.
[16] Asif AK,Sajjad AR,Thomas M. Assessment of salinity tolerance based upon seedling root growth response functions in maize[J]. Euphytica,2003,131:81-89.
[17] Sairam RK,Srivastava GC,Agarwal S,et al. Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes[J]. Biologia Plantarum,2005,49(1):85-91.
[18]刘爱荣,张远兵,方园园,等.盐胁迫对金盏菊生长、抗氧化能力和盐胁迫蛋白的影响[J].草业学报,2011,20(6):52-59.
[19]景艳霞,袁庆华. Na Cl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J].草业学报,2011,20(2):134-139.
[20]过晓明,李强,王欣,等.盐胁迫对甘薯幼苗生理特性的影响[J].江苏农业科学,2011,39(3):107-109.
[21]陈树钢,丁彦芬.以电导法配合Logistic方程确定5种景天属植物耐旱性[J].江苏农业科学,2011,39(3):213-216.
[22]吴嘉雯,王庆亚.干旱胁迫对野生和栽培蒲公英抗性生理生化指标的影响[J].江苏农业学报,2010,26(2):264-271.