盐蒿提取液抗食源性致病菌活性及其抑菌机理
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摘要
以5种常见的致病菌为试验对象,采用体外抑菌试验筛选出盐蒿提取液作用效果最好的致病菌,并通过研究盐蒿提取液对致病菌作用前后细菌培养液的OD260、电导率、碱性磷酸酶(AKP)活性和细胞超微结构的变化,初步探讨盐蒿提取液对单增李斯特菌(Listeria monocytogenes)的抑菌机制。结果显示,盐蒿提取液对5种致病菌均有抑菌活性,但对单增李斯特菌抑制效果最好,最小抑菌浓度(MIC)为0.062 5 mg/ml,对5种致病菌抑制效果表现为:单增李斯特菌>金黄色葡萄球菌>大肠杆菌>肠炎沙门氏菌>鲍氏志贺氏菌。单增李斯特菌经盐蒿提取液处理后,细菌培养液260 nm的吸光度、电导率和AKP活性均增大,表明盐蒿提取液可破坏单增李斯特菌细胞壁和细胞膜的结构,导致细胞通透性增加,进而使细胞内容物外泄,使细胞生长受到抑制,进而导致死亡。
Five kinds of food-derived pathogenic bacteria were used for the detection of antimicrobial activity of Artemisia halodendron extract,and the antimicrobial mechanisms were studied by determining the change of OD260,electric conductivity and alkaline phosphatase content of the culture solution for bacteria and the cell structure under scanning electron microscope of Listeria monocytogenes.A.halodendron extract exhibited inhibitory effects on Staphylococcus aureus,Escherichia coli,L.monocytogenes,Shigella bogdii and Salmonella.The minimum inhibitory concentration (MIC) against L.monocytogenes was 0.062 5 mg/ml.The antibacterial activity on the bacteria from strong to weak followed the order of L.monocytogene > S.aureus > E.coli > Salmonellai > S.bogdi.The increase of OD260,electric conductivity and alkaline phosphatase content of bacteria culture solution indicated that A.halodendron extract could damage the structure of cell wall and membrane,resulting in the increase of permeability of cell membrane and release of cell components,thereby inhibiting the growth of cell and causing death.
Five kinds of food-derived pathogenic bacteria were used for the detection of antimicrobial activity of Artemisia halodendron extract,and the antimicrobial mechanisms were studied by determining the change of OD260,electric conductivity and alkaline phosphatase content of the culture solution for bacteria and the cell structure under scanning electron microscope of Listeria monocytogenes.A.halodendron extract exhibited inhibitory effects on Staphylococcus aureus,Escherichia coli,L.monocytogenes,Shigella bogdii and Salmonella.The minimum inhibitory concentration (MIC) against L.monocytogenes was 0.062 5 mg/ml.The antibacterial activity on the bacteria from strong to weak followed the order of L.monocytogene > S.aureus > E.coli > Salmonellai > S.bogdi.The increase of OD260,electric conductivity and alkaline phosphatase content of bacteria culture solution indicated that A.halodendron extract could damage the structure of cell wall and membrane,resulting in the increase of permeability of cell membrane and release of cell components,thereby inhibiting the growth of cell and causing death.
引文
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[2]任伟重,姜华,郑音,等.碱蓬资源的开发价值[J].辽宁农业科学,2011(5):51-53.
[3]韩进萍,徐敏.江苏沿海滩涂开发利用评价[J].海洋开发与管理,2006,23(2):99-102.
[4]GLENN E P,JED BROWN J,O'LEARY J W.Irrigating crops with seawater[J].Scientific American,1998,279(2):76-81.
[5]AGATI G,AZZARELLO E,POLLASTRI S,et al.Flavonoids as antioxidants in plants:location and functional significance[J].Plant Science,2012,196:67-76.
[6]BONDONNO C P,YANG X,CROFT K D,et al.Flavonoid-rich apples and nitrate-rich spinach augment nitric oxide status and improve endothelial function in healthy men and women:a randomized controlled trial[J].Free Radical Biology and Medicine,2012,52(1):95-102.
[7]SEAL T.HPLC determination of phenolic acids,flavonoids and ascorbic acid in four different solvent extracts of Zanthoxylum acanthopodium,a wild edible plant of Meghalaya state of India[J].International Journal of Pharmacy&Pharmaceutical Sciences,2016,8(3):103-109.
[8]韦日明,高雅,张可锋,等.杠板归总黄酮对小鼠酒精性肝损伤的保护作用[J].南方农业学报,2016,47(4):664-669.
[9]董蒙蒙,喻樊,刘佳,等.中华补血草5种黄酮类化合物抗氧化和抗肿瘤活性的比较[J].江苏农业科学,2015,43(2):297-299.
[10]韩进萍,徐敏.江苏沿海滩涂开发利用评价[J].海洋开发与管理,2006,23(2):99-102.
[11]刘诗扬,马莲菊,卜宁.1株碱蓬内生芽孢杆菌的分离及抑菌活性测定[J].贵州农业科学,2011,39(3):135-138.
[12]赵学思,师仁丽,李岩,等.碱蓬黄酮提取物的体外抗氧化及抑菌性研究[J].食品工业科技,2016,37(13):63-71.
[13]武运,李远,王冰峰,等.新疆酸驼乳中细菌素乳酸菌的筛选及其抑菌性[J].食品与机械,2011,27(3):25-28.
[14]张添菊,王帆,李春阳,等.蓝莓叶多酚组成及其抗食源性致病菌活性分析[J].食品科学,2016,37(23):95-99.
[15]王帆,杨静东,王春梅,等.肉桂醛对大肠杆菌和绿脓杆菌的作用机制[J].江苏农业学报,2011,27(4):888-892.
[16]LOU Z,WANG H,ZHU S,et al.Antibacterial activity and mechanism of action of chlorogenic acid[J].Journal of Food Science,2011,76(6):M398-M403.
[17]ZHANG X H,WANG Z,YIN B,et al.A complex of trypsin and chymotrypsin effectively inhibited growth of pathogenic bacteria inducing cow mastitis and showed synergistic antibacterial activity with antibiotics[J].Livestock Science,2016,188:25-36.
[18]FENG H,YING Y,YANG G A,et al.Studies on antibacterial activity and antibacterial mechanism of a novel polysaccharide from Streptomyces virginia H03[J].Food Control,2010,21(9):1257-1262.
[19]张赟彬,刘笑宇,姜萍萍,等.肉桂醛对大肠杆菌和金黄色葡萄球菌的抑菌作用及抑菌机理研究[J].现代食品科技,2015,31(5):31-35.
[20]DIAO W R,HU Q P,ZHANG H,et al.Chemical composition,antibacterial activity and mechanism of action of essential oil from seeds of fennel(Foeniculum vulgare,Mill.)[J].Food Control,2014,35(1):109-116.
[21]陈峰,田维娜,孙东哲,等.微藻对微生物的抑制作用及其抑菌机理[J].中国食品学报,2014,14(4):1-9.
[22]曾哲灵,赵存洋,罗春燕,等.单癸月桂酸甘油酯的抑菌作用及机理研究[J].食品科学,2013,34(3):70-74.
[23]谢晶,侯伟峰,汤毅,等.植酸对腐败希瓦氏菌的抑菌机理[J].食品工业科技,2011(10):85-88.
[24]刘唤明,孙力军,王雅玲,等.纳豆菌脂肽对金黄色葡萄球菌抑菌机理的研究[J].食品工业科技,2012,33(11):109-112.
[25]CUI H Y,ZHOU H,LIN L,et al.Antibacterial activity and mechanism of cinnamon essential oil and its application in milk[J].Japs Journal of Animal&Plant Sciences,2016,26(2):532-541.