Detection of Drug Resistance in Escherichia coli Isolated from Animals and ERIC Analysis of Multidrug-resistant Strains
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摘要
The drug resistance of Escherichia coli from animals was detected in Shandong Province in 2016, the gene mapping of Enterobacterial Repetitive Intergenic Consensus-PCR(ERIC-PCR) of multidrug-resistant isolates were analyzed, and then the relationship between ERIC-PCR genotyping and drug resistance of highly-resistant E. coli with multidrug resistance was discussed. A total of 110 E. coli isolates were separated and identified from diseased swine and avian, and their resistance to 10 kinds of antimicrobials was determined by the agar dilution method. Twenty highly-resistant isolates with multidrug resistance were selected to carry on the ERIC-PCR, followed by cluster genetic analysis according to the DNA fingerprints. The swine-sourced E. coli isolates possessed serious resistance against several kinds of antimicrobial agents, for example, all isolates were tolerant to florfenicol, doxycycline and ampicillin(100%), but had a relative lower resistance rate to cefotaxime sodium(57.14%). The same situation was observed in the poultry-sourced E. coli isolates, which had a resistance rate of 95.51% against florfenicol, while the lowest rate of 61.80% appeared on ciprofloxacin. Analysis of ERTIC showed dispersive fingerprint patterns of highly-resistant and multidrug-resistant isolates which represented a multiple clone resources, and there were certain correlation between the B genotype and the drug-resistant characteristics. It indicated that the animal-sourced E. coli isolates had a high level of drug resistance and were multidrug-resistant, which meant there was severe antibiotic resistance against not only different kinds of antibiotics but also different drugs of the same kind. The highly-resistant E. coli isolates with multidrug resistance had no apparent species preference, while their spread and pervasion posed a potential threat to the development of animal husbandry and the public health security.
The drug resistance of Escherichia coli from animals was detected in Shandong Province in 2016, the gene mapping of Enterobacterial Repetitive Intergenic Consensus-PCR(ERIC-PCR) of multidrug-resistant isolates were analyzed, and then the relationship between ERIC-PCR genotyping and drug resistance of highly-resistant E. coli with multidrug resistance was discussed. A total of 110 E. coli isolates were separated and identified from diseased swine and avian, and their resistance to 10 kinds of antimicrobials was determined by the agar dilution method. Twenty highly-resistant isolates with multidrug resistance were selected to carry on the ERIC-PCR, followed by cluster genetic analysis according to the DNA fingerprints. The swine-sourced E. coli isolates possessed serious resistance against several kinds of antimicrobial agents, for example, all isolates were tolerant to florfenicol, doxycycline and ampicillin(100%), but had a relative lower resistance rate to cefotaxime sodium(57.14%). The same situation was observed in the poultry-sourced E. coli isolates, which had a resistance rate of 95.51% against florfenicol, while the lowest rate of 61.80% appeared on ciprofloxacin. Analysis of ERTIC showed dispersive fingerprint patterns of highly-resistant and multidrug-resistant isolates which represented a multiple clone resources, and there were certain correlation between the B genotype and the drug-resistant characteristics. It indicated that the animal-sourced E. coli isolates had a high level of drug resistance and were multidrug-resistant, which meant there was severe antibiotic resistance against not only different kinds of antibiotics but also different drugs of the same kind. The highly-resistant E. coli isolates with multidrug resistance had no apparent species preference, while their spread and pervasion posed a potential threat to the development of animal husbandry and the public health security.
The drug resistance of Escherichia coli from animals was detected in Shandong Province in 2016, the gene mapping of Enterobacterial Repetitive Intergenic Consensus-PCR(ERIC-PCR) of multidrug-resistant isolates were analyzed, and then the relationship between ERIC-PCR genotyping and drug resistance of highly-resistant E. coli with multidrug resistance was discussed. A total of 110 E. coli isolates were separated and identified from diseased swine and avian, and their resistance to 10 kinds of antimicrobials was determined by the agar dilution method. Twenty highly-resistant isolates with multidrug resistance were selected to carry on the ERIC-PCR, followed by cluster genetic analysis according to the DNA fingerprints. The swine-sourced E. coli isolates possessed serious resistance against several kinds of antimicrobial agents, for example, all isolates were tolerant to florfenicol, doxycycline and ampicillin(100%), but had a relative lower resistance rate to cefotaxime sodium(57.14%). The same situation was observed in the poultry-sourced E. coli isolates, which had a resistance rate of 95.51% against florfenicol, while the lowest rate of 61.80% appeared on ciprofloxacin. Analysis of ERTIC showed dispersive fingerprint patterns of highly-resistant and multidrug-resistant isolates which represented a multiple clone resources, and there were certain correlation between the B genotype and the drug-resistant characteristics. It indicated that the animal-sourced E. coli isolates had a high level of drug resistance and were multidrug-resistant, which meant there was severe antibiotic resistance against not only different kinds of antibiotics but also different drugs of the same kind. The highly-resistant E. coli isolates with multidrug resistance had no apparent species preference, while their spread and pervasion posed a potential threat to the development of animal husbandry and the public health security.
引文
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[14]HE DD,HUANG LZ,CHEN XJ,et al.Investigation of antibiotic resistance among Escherichia coli isolated from different animals[J].China Animal Husbandry and Veterinary Medicine,2013,40(10):211-215.(in Chinese)
[15]ZHANG CP,NING YB,SONG L.Resistance to tetracycline and distribution of tetracycline resistance determinants in commensal Escherichia coli isolated from clinically healthy chickens and pigs[J].Scientia Agricultura Sinica,2010,43(12):2578-2583.(in Chinese)
[16]ZHAO J,YANG HC,ZHA XL.Monitoring of Escherichia coli resistance in large-scale pig farms[J].Chinese Journal of Veterinary Medicine,1998,24(11):12-13.(in Chinese)
[17]ZHANG XL,LI JT.Distribution and expression of active efflux system in Escherichia coli[J].The Chinese Journal of Clinical Pharmacology,1999,15(3):171-174.(in Chinese)
[18]LI YH,QU F,BAO CM,et al.Resistance and cross-resistance of intestinal bacteria to fluoroquinolones[J].Chinese Journal of Antibiotics,2009(4):251-253.(in Chinese)
[19]BARRY AL,JONES RN.Cross-resistance among cinoxacin,ciprofloxacin,DJ-6783,enoxacin,nalidixic acid,no-rfloxacin,and oxolinic acid after in vitro selection of resistant populations[J].Antimicrob.Agents Chemo.Ther.,1984,25:775-777.
[2]AN WEI,ZHANG XY,LI R,et al.Advances in research on pathogenic Escherichia coli virulence factors and drug resistance[J].Animal Husbandry and Veterinary Medicine,2013,45(8):106-110.(in Chinese)
[3]TITILAWO Y,SIBANDA T,OBI L,et al.Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of faecal contamination of water[J].Environ.Sci.Pollut.Res.Int.,2015,22:10969-10980.
[4]LIN JC,ZHUO JZ,JIANG HX,et al.Surveillance of antimicrobial resistance among Escherichia coli isolates from swine and poultry in different regions[J].Journal of South China Agricultural University,2009,30(1):86-88.(in Chinese)()
[5]HULTON CS,HIGGNSC F.ERIC sequences:a novel family of repetitive elements in the genomes of Escherichia coli,Salmonella typhimurium and other enterobacteria[J].Journal of Molecular Microbiology and Biotechnology,1991,5:825-830.
[6]Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing:18th informational supplement[S].CLSI Document M100-S18,2008.
[7]VERSALOVIC J,KOEUTH T,LUPSKI JR.Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial genomes[J].Nucleic Acids Research,1991,19(24):6823-6831.
[8]SANDVANG D,AARESTRUP FM,JENSEN LB.Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimutium DT104[J].FEMS Microbiology Letters,1998,160(1):37-41.
[9]ZHANG JF,LI YL,CHEN XH,et al.Studies on cross-resistance of avain Escherichia coli to fluoroquinolones[J].Guangdong Agricultural Sciences,2005(2):74-76.(in Chinese)
[10]CHAIEB K,ABBASSI MS,TOUATI A,et al.Molecular characterization of Staphylococcus epidermidis isolated from biomaterials in a dialysis service[J].Annals of Microbiology,2005,55:307-312.
[11]MATHAI E,CHANDY S,THOMAS K,et al.Antimicrobial resistance surveillance among commensal Escherichia coli in rural and urban areas in Southern India[J].Trop.Med.Int.Health,2008,13(1):41-45.
[12]LEI LC,ZHENG D,HAN WY,et al.Antibiotic sensitivity detection and analysis of Escherichia coli isolated clinically in China[J].Chinese Journal of Veterinary Science,2005,25(5):470-473.(in Chinese)
[13]SUI H,YANG JS.Isolation and identification of animal-derived multidrug-resistant Escherichia coli[J].Chinese Journal of Veterinary Medicine,2013,49(6):48-50.(in Chinese)
[14]HE DD,HUANG LZ,CHEN XJ,et al.Investigation of antibiotic resistance among Escherichia coli isolated from different animals[J].China Animal Husbandry and Veterinary Medicine,2013,40(10):211-215.(in Chinese)
[15]ZHANG CP,NING YB,SONG L.Resistance to tetracycline and distribution of tetracycline resistance determinants in commensal Escherichia coli isolated from clinically healthy chickens and pigs[J].Scientia Agricultura Sinica,2010,43(12):2578-2583.(in Chinese)
[16]ZHAO J,YANG HC,ZHA XL.Monitoring of Escherichia coli resistance in large-scale pig farms[J].Chinese Journal of Veterinary Medicine,1998,24(11):12-13.(in Chinese)
[17]ZHANG XL,LI JT.Distribution and expression of active efflux system in Escherichia coli[J].The Chinese Journal of Clinical Pharmacology,1999,15(3):171-174.(in Chinese)
[18]LI YH,QU F,BAO CM,et al.Resistance and cross-resistance of intestinal bacteria to fluoroquinolones[J].Chinese Journal of Antibiotics,2009(4):251-253.(in Chinese)
[19]BARRY AL,JONES RN.Cross-resistance among cinoxacin,ciprofloxacin,DJ-6783,enoxacin,nalidixic acid,no-rfloxacin,and oxolinic acid after in vitro selection of resistant populations[J].Antimicrob.Agents Chemo.Ther.,1984,25:775-777.