变色栓菌对直接大红染料的脱色研究
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摘要
从野外倒木上分离得到一株对直接染料具有高效脱色能力的白腐真菌CB1,采用形态学与分子生物学鉴定法对菌株CB1进行鉴定.结果表明,菌株CB1为变色栓菌(Trametes versicolor);在固体培养基上,利用T.versicolor CB1对5种直接染料进行脱色,结果显示,T.versicolor CB1对5种直接染料均可脱色,其中对直接大红的脱色效果最为明显;采用单因素分析方法对T.versicolor CB1脱色直接大红的培养条件进行优化,结果显示,以果糖为碳源、硝酸铵为氮源、p H=5、添加Cu SO_4和Ca Cl_2利于T.versicolor CB1对直接大红的脱色;通过正交试验优化T.versicolor CB1对直接大红的脱色条件,优化结果为:果糖10 g·L~(-1)、硝酸铵2 g·L~(-1)、Cu SO_41 mmol·L~(-1)、Ca Cl_23 mmol·L~(-1)、p H=5.优化条件下第7 d时的脱色率为78.23%,与优化前相比,脱色率提高了24.04%.
A white rot fungus strain CB1,which had high ability to decolorize direct dyes,was isolated from fallen logs in the field.The strain CB1 was identified by using morphological and molecular biology methods.The results show that the strain CB1 was Trametes versicolor.In this study,we also found that all the 5 kinds of direct dyes could be decolorized by T.versicolor CB1.And the decolorization performance of direct red was the most obvious.the culture condition of T.versicolor CB1 decolorizing direct red was optimized by single-factor analysis,and the optimum medium contained fructose as carbon source,ammonium nitrate as nitrogen source,Cu SO_4and Ca Cl_2,and the appropriate p H was 5.The optimized culture condition of orthogonal experiment was fructose 10 g·L~(-1),ammonium nitrate 2 g·L~(-1),Cu SO_41 mmol·L~(-1),Ca Cl_23 mmol·L~(-1),p H=5.Under the optimum culture condition,the decolorization rate of direct red by T.versicolor CB1 reached 78.23%at 7 d,and increased by 24.04%compared with the non-optimized culture condition.
A white rot fungus strain CB1,which had high ability to decolorize direct dyes,was isolated from fallen logs in the field.The strain CB1 was identified by using morphological and molecular biology methods.The results show that the strain CB1 was Trametes versicolor.In this study,we also found that all the 5 kinds of direct dyes could be decolorized by T.versicolor CB1.And the decolorization performance of direct red was the most obvious.the culture condition of T.versicolor CB1 decolorizing direct red was optimized by single-factor analysis,and the optimum medium contained fructose as carbon source,ammonium nitrate as nitrogen source,Cu SO_4and Ca Cl_2,and the appropriate p H was 5.The optimized culture condition of orthogonal experiment was fructose 10 g·L~(-1),ammonium nitrate 2 g·L~(-1),Cu SO_41 mmol·L~(-1),Ca Cl_23 mmol·L~(-1),p H=5.Under the optimum culture condition,the decolorization rate of direct red by T.versicolor CB1 reached 78.23%at 7 d,and increased by 24.04%compared with the non-optimized culture condition.
引文
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Revankar M S,Lele S S.2007.Synthetic dye decolorization by white rot fungus,Ganoderma sp.WR-1[J].Bioresour Technology,98(4):775-780
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王慧,周月霞,柏仕杰,等.2008.染料废水生物法处理技术的研究进展[J].厦门大学学报(自然科学版),47(2):286-290
于存,池玉杰.2017.一色齿毛菌CB1对活性染料的脱色研究[J].菌物学报,36(6):743-751
张旭,陈胜,孙德智,等.2009.印染废水生物法处理技术研究进展[J].长春工业大学学报(自然科学版),30(1):26-32
Glenn J K,Gold M H.1983.Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phanerochaete chrysosporium[J].Applied and Environmental Microbiology,45(6):1741-1747
何艳喜.2010.Trametes versicolor HN0511的鉴定及其产木聚糖酶的分离纯化、性质分析和基因的克隆[D].广州:暨南大学
金敏,李君文.2003.白腐菌处理染料废水的研究进展[J].环境污染治理技术与设备,4(3):54-58
马俊,梁彦秋,孙小寒.2011.十六烷基三甲基溴化铵改性粉煤灰吸附酸性大红染料[J].辽宁化工,40(9):901-903
Levin L,Papinutti L,Forchiassin F.2004.Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes[J].Bioresour Technology,94(2):169-176
梁宏,曾抗美.2003.染料废水处理方法的研究进展[J].四川轻化工学院学报,16(2):20-24
刘转年,赵西成.2007.粉煤灰廉价吸附剂资源化利用的现状和对策分析[J].西安建筑科技大学学报(自然科学版),39(5):690-694
由庆.2016.产漆酶菌株的筛选及漆酶基因的克隆表达[D].哈尔滨:哈尔滨商业大学
任南琪,周显娇,郭婉茜,等.2013.染料废水处理技术研究进展[J].化工学报,64(1):84-94
Revankar M S,Lele S S.2007.Synthetic dye decolorization by white rot fungus,Ganoderma sp.WR-1[J].Bioresour Technology,98(4):775-780
王慧,郑小伟,王宾香,等.2009.真菌对染料的脱色研究进展[J].应用环境生物学报,15(1):147-151
王慧,周月霞,柏仕杰,等.2008.染料废水生物法处理技术的研究进展[J].厦门大学学报(自然科学版),47(2):286-290
于存,池玉杰.2017.一色齿毛菌CB1对活性染料的脱色研究[J].菌物学报,36(6):743-751
张旭,陈胜,孙德智,等.2009.印染废水生物法处理技术研究进展[J].长春工业大学学报(自然科学版),30(1):26-32