海南黎族胃癌患者血清P53蛋白、sFas的检测及临床意义
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摘要
[目的]探讨海南黎族胃癌患者血清P53蛋白、可溶性Fas蛋白(sFas)的检测及临床意义。[方法]选取2017年1月～2018年4月我院收治的海南地区黎族慢性浅表性胃炎患者82例、慢性萎缩性胃炎患者82例、胃癌患者82例为研究对象,分别采集3组患者的血液标本,应用ELISA法分别检测3组患者血清P53蛋白和sFas浓度,探讨其表达水平与胃癌患者临床病理特征的关系,并分析胃癌患者血清P53蛋白与sFas的相关性。[结果]胃癌组患者血清P53蛋白浓度明显高于慢性浅表性胃炎组和慢性萎缩性胃炎组(P<0.05);胃癌组患者血清sFas浓度明显低于慢性浅表性胃炎组和慢性萎缩性胃炎组(P<0.05);血清P53蛋白和sFas与胃癌TNM分期、浸润程度及淋巴结转移密切相关(P <0.05),而与患者性别、年龄、肿瘤直径、病理分型及分化程度无明显关系(P>0.05);Pearson相关性分析结果显示,胃癌患者血清P53蛋白与sFas呈负相关(P<0.05)。[结论]海南黎族胃癌患者血清中P53蛋白呈高表达,而sFas呈低表达,P53蛋白和sFas与胃癌的发生、发展存在相关性,或将成为胃癌早期诊断及预后评价的新指标。
[Objective]To investigate the serum levels of P53 protein and soluble Fas protein(sFas)in patients with gastric cancer in Li nationality in Hainan and their clinical significance.[Methods]From January 2017 to April 2018 in our hospital,82 cases of chronic superficial gastritis of the Li nationality,82 cases of chronic atrophic gastritis,and 82 cases of gastric cancer were selected as subjects for study in the Department of Gastroenterology of our hospital.Blood samples from 3 groups were collected separately.To explore the relationship between P53 protein expression level and clinicopathological features of gastric cancer patients.The serum levels of P53 protein and sFas were respectively detected by ELISA in three groups.And the correlation between serum P53 protein and sFas in patients with gastric cancer was also analyzed.[Results]The serum P53 protein concentration in patients with gastric cancer was significantly higher than that in chronic superficial gastritis and chronic atrophic gastritis(P<0.05).The serum sFas concentration in patients with gastric cancer was significantly lower than that in chronic superficial gastritis and chronic atrophic gastritis(P<0.05).Serum P53 protein and sFas were closely related to TNM staging,invasive degree and lymph node metastasis of gastric cancer(P<0.05),and there was no significant relationship with gender,age,tumor diameter,pathological type and differentiation.Pearson correlation analysis showed that serum P53 protein was negatively correlated with sFas in gastric cancer patients(P<0.05).[Conclusion]The expression of P53 protein in serum of patients with gastric cancer in Li nationality in Hainan was high,while sFas is low expression,P53 protein and sFas are related to the occurrence and development of gastric cancer,and it will become a new indicator for early diagnosis and prognosis evaluation of gastric cancer.
[Objective]To investigate the serum levels of P53 protein and soluble Fas protein(sFas)in patients with gastric cancer in Li nationality in Hainan and their clinical significance.[Methods]From January 2017 to April 2018 in our hospital,82 cases of chronic superficial gastritis of the Li nationality,82 cases of chronic atrophic gastritis,and 82 cases of gastric cancer were selected as subjects for study in the Department of Gastroenterology of our hospital.Blood samples from 3 groups were collected separately.To explore the relationship between P53 protein expression level and clinicopathological features of gastric cancer patients.The serum levels of P53 protein and sFas were respectively detected by ELISA in three groups.And the correlation between serum P53 protein and sFas in patients with gastric cancer was also analyzed.[Results]The serum P53 protein concentration in patients with gastric cancer was significantly higher than that in chronic superficial gastritis and chronic atrophic gastritis(P<0.05).The serum sFas concentration in patients with gastric cancer was significantly lower than that in chronic superficial gastritis and chronic atrophic gastritis(P<0.05).Serum P53 protein and sFas were closely related to TNM staging,invasive degree and lymph node metastasis of gastric cancer(P<0.05),and there was no significant relationship with gender,age,tumor diameter,pathological type and differentiation.Pearson correlation analysis showed that serum P53 protein was negatively correlated with sFas in gastric cancer patients(P<0.05).[Conclusion]The expression of P53 protein in serum of patients with gastric cancer in Li nationality in Hainan was high,while sFas is low expression,P53 protein and sFas are related to the occurrence and development of gastric cancer,and it will become a new indicator for early diagnosis and prognosis evaluation of gastric cancer.
引文
[1]闵丛丛,张静,丁士刚.胃癌淋巴结转移分子标志物的研究进展[J].中国微创外科杂志,2018,7(1):68-71.
[2]郭洪胜,郑妮,邓子亮,等.胃癌患者三级淋巴组织的分布及其与患者预后的关系和意义[J].解剖学杂志,2018,4(1):23-27.
[3]尚自强,童莹,宋佳,等.肺腺癌组织中RNA结合基序蛋白38和p53基因的表达及其临床意义[J].中国肿瘤生物治疗杂志,2017,24(8):900-903.
[4]刘曼,胡明英,王晓莉,等.AKT基因沉默对人卵巢癌细胞增殖及Fas、Fas L、Caspase-3蛋白表达的影响[J].山东医药,2016,13(7):29-31.
[5]樊平,张扬,邓芳,等.血清人肿瘤蛋白P53在结直肠癌联合诊断及术后监测中的应用价值[J].中华医学杂志,2017,97(34):174-176.
[6]嵇晓辉,李军,杨金花.SATB2和P53蛋白在结肠癌组织中的表达及临床意义[J].实用医学杂志,2017,11(23):3945-3948.
[7]程智勇,杨雁鸿,李桃,等.Tip30和p53蛋白在胃癌组织中的表达及其临床意义[J].疑难病杂志,2016,15(8):813-816.
[8]王娜,徐小艳,张思森,等.NEDD9、Ki67、P53联合检测对胃癌的早期诊断和恶性程度评估的价值[J].实用医学杂志,2017,33(12):1961-1965.
[9]孙桂丽,任志红,王大鹏,等.p53在食管癌中的研究进展[J].现代肿瘤医学,2016,24(15):2487-2490.
[10]甘露,许嵩.PTEN和P53蛋白与子宫内膜癌发生发展的相关性研究[J].临床和实验医学杂志,2016,15(21):2114-2117.
[11]王一,廖宏伟,宋扬.三阴型乳腺癌患者血清Vit D水平与瘤组织Ki-67、p53蛋白表达变化及其相关性[J].山东医药,2018,9(11):1073-1074.
[12]邓亚平,钟晶敏,李晶,等.Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理研究[J].现代生物医学进展,2016,16(26):5068-5071.
[13]佐晶,宋艳,李卓,等.P53蛋白表达与晚期卵巢浆液性腺癌预后的相关性[J].中国医学科学院学报,2016,38(2):169-174.
[14]陈晓露,张波,吴蓉宜.CK2β、VEGF和p53蛋白表达对宫颈癌患者预后的预测价值研究[J].重庆医学,2016,45(8):1083-1086.
[15]杨惠祥,郭勇,王振潮,等.尿可溶性Fas和血管内皮生长因子在膀胱癌中表达的意义及相关性研究[J].中国现代医学杂志,2015,25(5):37-40.
[16]王立平,张正银,郭振华,等.胃癌患者血清CXCL12、TPS、sFas的表达和临床意义[J].临床和实验医学杂志,2014,11(17):1431-1433.
[17]帕拉提·吐尔逊,热孜亚·艾尼,木合塔尔·霍加.P53,Ki-67蛋白在口腔鳞癌中的表达意义及与预后的关系[J].口腔医学研究,2016,13(1):87-90.
[2]郭洪胜,郑妮,邓子亮,等.胃癌患者三级淋巴组织的分布及其与患者预后的关系和意义[J].解剖学杂志,2018,4(1):23-27.
[3]尚自强,童莹,宋佳,等.肺腺癌组织中RNA结合基序蛋白38和p53基因的表达及其临床意义[J].中国肿瘤生物治疗杂志,2017,24(8):900-903.
[4]刘曼,胡明英,王晓莉,等.AKT基因沉默对人卵巢癌细胞增殖及Fas、Fas L、Caspase-3蛋白表达的影响[J].山东医药,2016,13(7):29-31.
[5]樊平,张扬,邓芳,等.血清人肿瘤蛋白P53在结直肠癌联合诊断及术后监测中的应用价值[J].中华医学杂志,2017,97(34):174-176.
[6]嵇晓辉,李军,杨金花.SATB2和P53蛋白在结肠癌组织中的表达及临床意义[J].实用医学杂志,2017,11(23):3945-3948.
[7]程智勇,杨雁鸿,李桃,等.Tip30和p53蛋白在胃癌组织中的表达及其临床意义[J].疑难病杂志,2016,15(8):813-816.
[8]王娜,徐小艳,张思森,等.NEDD9、Ki67、P53联合检测对胃癌的早期诊断和恶性程度评估的价值[J].实用医学杂志,2017,33(12):1961-1965.
[9]孙桂丽,任志红,王大鹏,等.p53在食管癌中的研究进展[J].现代肿瘤医学,2016,24(15):2487-2490.
[10]甘露,许嵩.PTEN和P53蛋白与子宫内膜癌发生发展的相关性研究[J].临床和实验医学杂志,2016,15(21):2114-2117.
[11]王一,廖宏伟,宋扬.三阴型乳腺癌患者血清Vit D水平与瘤组织Ki-67、p53蛋白表达变化及其相关性[J].山东医药,2018,9(11):1073-1074.
[12]邓亚平,钟晶敏,李晶,等.Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理研究[J].现代生物医学进展,2016,16(26):5068-5071.
[13]佐晶,宋艳,李卓,等.P53蛋白表达与晚期卵巢浆液性腺癌预后的相关性[J].中国医学科学院学报,2016,38(2):169-174.
[14]陈晓露,张波,吴蓉宜.CK2β、VEGF和p53蛋白表达对宫颈癌患者预后的预测价值研究[J].重庆医学,2016,45(8):1083-1086.
[15]杨惠祥,郭勇,王振潮,等.尿可溶性Fas和血管内皮生长因子在膀胱癌中表达的意义及相关性研究[J].中国现代医学杂志,2015,25(5):37-40.
[16]王立平,张正银,郭振华,等.胃癌患者血清CXCL12、TPS、sFas的表达和临床意义[J].临床和实验医学杂志,2014,11(17):1431-1433.
[17]帕拉提·吐尔逊,热孜亚·艾尼,木合塔尔·霍加.P53,Ki-67蛋白在口腔鳞癌中的表达意义及与预后的关系[J].口腔医学研究,2016,13(1):87-90.