FT-IR结合相关系数法对不同产地艾叶挥发油的比较分析
|
摘要
为建立艾叶挥发油的红外光谱快速鉴别方法,本试验采用水蒸气蒸馏法分别提取不同来源产地艾叶中的挥发油,结合傅里叶变换红外光谱(FT-IR)技术对各挥发油样品进行了红外光谱的测定,并通过相关系数法对不同产地来源艾叶中的挥发油成分进行归类。结果表明,艾叶挥发油中主要含有醇类、烯类、醛酮类、酯类等化合物,与文献报道中用GCMS方法分析艾叶中的挥发油所得成分相一致。设定相关系数阈值0.9500以区分河南产地与其他产地的艾叶药材,设定相关系数阈值0.9000以区分艾叶和艾花不同的药用部位。结论:采用FT-IR技术结合相关系数法,可对艾叶挥发油进行快速分析,为艾叶挥发油的质量评价提供了科学依据。
To establish the rapid identification method for volatile oil of argy wormwood leaf on Fourier Transform Infrared Spectroscopy( FT-IR),essential oils were extracted from different argy wormwood leaf samples selected from different origins by hydrodistillation extraction The infrared spectrograms of essential oils were determined with FT-IR,and the chemical components of essentials oils were also classified by correlation coefficient method. The results showed that the main components in the essential oils extracted from argy wormwood leaf samples were alcohols,alkenes,keto aldehyde and esters,etc.,which were consistent with that determined by GC-MS in literature reports. The correlation coefficient threshold 0. 9500 was set to distinguish argy wormwood leaves from Henan origin with others,while 0. 9000 was set to distinguish argy wormwood leaves with their blossoms. The volatile oil of argy wormwood leaf could be analyzed rapidly by using the FT-IR technology and correlation coefficient method.
To establish the rapid identification method for volatile oil of argy wormwood leaf on Fourier Transform Infrared Spectroscopy( FT-IR),essential oils were extracted from different argy wormwood leaf samples selected from different origins by hydrodistillation extraction The infrared spectrograms of essential oils were determined with FT-IR,and the chemical components of essentials oils were also classified by correlation coefficient method. The results showed that the main components in the essential oils extracted from argy wormwood leaf samples were alcohols,alkenes,keto aldehyde and esters,etc.,which were consistent with that determined by GC-MS in literature reports. The correlation coefficient threshold 0. 9500 was set to distinguish argy wormwood leaves from Henan origin with others,while 0. 9000 was set to distinguish argy wormwood leaves with their blossoms. The volatile oil of argy wormwood leaf could be analyzed rapidly by using the FT-IR technology and correlation coefficient method.
引文
[1]国家药典委员会.中华人民共和国药典[S].四部.2015:203-204.
[2]陈小露,梅全喜.艾叶化学成分研究进展[J].今日药学,2013,23(12):848-851.
[3]梁欢,卢金清,戴艺,等.HS-SPME-GC-MS结合化学计量法对不同产地艾叶药材挥发性成分的比较分析[J].中国实验方剂学杂志,2014,20(18):85-90.
[4]张桂芝,孟庆华.四种姜科中药挥发油的红外指纹图谱研究[J].现代中药研究与实践,2014,28(4):14-16.
[5]王斌,任西杰,王燕,等.基于聚类、主成分和判别分析的海马醇提物红外指纹图谱研究[J].中国药学杂志,2013,48(4):253-258.
[6]洪宗国,魏海胜,张令令,等.不同采集期艾叶挥发油含量和化学成分的研究[J].中南民族大学学报(自然科学版),2013,32(2):32-35.
[2]陈小露,梅全喜.艾叶化学成分研究进展[J].今日药学,2013,23(12):848-851.
[3]梁欢,卢金清,戴艺,等.HS-SPME-GC-MS结合化学计量法对不同产地艾叶药材挥发性成分的比较分析[J].中国实验方剂学杂志,2014,20(18):85-90.
[4]张桂芝,孟庆华.四种姜科中药挥发油的红外指纹图谱研究[J].现代中药研究与实践,2014,28(4):14-16.
[5]王斌,任西杰,王燕,等.基于聚类、主成分和判别分析的海马醇提物红外指纹图谱研究[J].中国药学杂志,2013,48(4):253-258.
[6]洪宗国,魏海胜,张令令,等.不同采集期艾叶挥发油含量和化学成分的研究[J].中南民族大学学报(自然科学版),2013,32(2):32-35.