台湾相思内生真菌的分离筛选及其对幼苗生长的影响
|
摘要
为促进沿海防护林树种台湾相思的生长发育,分离其内生真菌后筛选出强势菌株A(Trichoderma sp.Pd1)、B(Filobasidium sp. As3)、C(Fusarium sp. Pd2)、D(Colletotrichum sp. Ys6)、E(Fusarium sp. Pd4),并将不同菌株及菌株组合浇根回接于幼苗,测定其对台湾相思幼苗生长指标与叶绿素质量分数差异。结果表明:菌株AE、E、AD、CD和CE对植株苗高生长有极显著的促进作用;大部分菌株对植株地径生长均有促进作用,其中菌株AB、AE、DE、AD作用最为显著;菌株B和C极显著的增加了植株的生物量和根冠比;菌株CE在试验菌株中对台湾相思幼苗光合作用的影响最佳。总之,菌株A、C、E、AE、CD对台湾相思幼苗所测各项生理指标均有促进作用,AE对其促生效果最佳。
To promote the growth and development of the Acacia confusa Merr. in coastal protection forest tree species,the strong strains were screened out after isolating the endophytic fungus: A( Trichoderma sp. Pd1),B( Filobasidium sp. As3),C(Fusarium sp. Pd2),D(Colletotrichum sp. Ys6) and E(Fusarium sp. Pd4),and the root casted bacteria were returned to the seedlings to determine the difference between the growth index and the chlorophyll content of the A. confusa seedlings. The strains AE,E,AD,CD and CE significantly promoted the growth of plant seedlings; most of the strains had the promotion effect on the growth of plant ground diameter,of which the strain AB,AE,DE and AD were the most significant; the strain B and C significantly increased the plant biomass and root and crown ratio; strain CE in the experimental strains had the best effect on the photosynthesis of A. confusa seedlings. The strains A,C,E,AE and CD could promote the physiological indexes of A. confuse seedlings,and AE had the best promoting effect on them.
To promote the growth and development of the Acacia confusa Merr. in coastal protection forest tree species,the strong strains were screened out after isolating the endophytic fungus: A( Trichoderma sp. Pd1),B( Filobasidium sp. As3),C(Fusarium sp. Pd2),D(Colletotrichum sp. Ys6) and E(Fusarium sp. Pd4),and the root casted bacteria were returned to the seedlings to determine the difference between the growth index and the chlorophyll content of the A. confusa seedlings. The strains AE,E,AD,CD and CE significantly promoted the growth of plant seedlings; most of the strains had the promotion effect on the growth of plant ground diameter,of which the strain AB,AE,DE and AD were the most significant; the strain B and C significantly increased the plant biomass and root and crown ratio; strain CE in the experimental strains had the best effect on the photosynthesis of A. confusa seedlings. The strains A,C,E,AE and CD could promote the physiological indexes of A. confuse seedlings,and AE had the best promoting effect on them.
引文
[1] ARNOLD A E,MAYNARD Z,GILBERT G S,et al. Are tropical fungal endophytes hyper diverse?[J]. Ecology Letters,2000,3(4):267-274.
[2] GUO L D,HUANG G R,WANG Y. Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis(Pinaceae)in the Dong ling Mountains,Beijing[J]. Journal of Integrative Plant Biology,2008,50(8):997-1003.
[3] BREM D,LEUCHTMANN A. Intraspecific competition of endophyte infected vs uninfected plants oftwowoodland grass species[J]. Oikos,2002,96(2):281-290.
[4] CLAY K,SCHARDL C. Evolutionary origins and ecological consequences of endophyte symbiosis with grasses[J]. The American Naturalist,2002,160(S4):S99-S127.
[5]胡美玲,郑勇,孙翔,等.内生真菌促进玉米抗旱性研究[J].菌物学报,2017,36(11):1556-1565.
[6]张炜银,王伯荪,李鸣光,等.台湾相思林和芒草草丛中薇甘菊枝构件的分枝格局及其生物量[J].植物生态学报,2002,26(3):346-350.
[7]肖泽鑫,彭剑华,詹潮安,等.主成分分析在台湾相思优树选择标准和方法中的应用研究[J].中南林业科技大学学报,2012,32(5):54-58.
[8]周锦业,官国栋,林宇,等.东南沿海不同相思树种叶绿素荧光特性的季相变化[J].西南林业大学学报,2014,34(1):21-26.
[9]陈国华,陈海艳,李宝福,等.沿海沙地木麻黄更新迹地6种相思树的水源涵养功能比较[J].防护林科技,2012(2):1-4.
[10]吴晓菡,李文超,秦路平.天目山山胡椒不同部位内生真菌组成及多样性分析[J].植物资源与环境学报,2012(2):107-113.
[11]崔林,孙振,孙福在,等.马铃薯内生细菌的分离及环腐病拮抗菌的筛选鉴定[J].植物病理学报,2003,33(4):353-358.
[12]龚贺,张雷鸣,任伟超,等.膜荚黄芪内生真菌的分离与鉴定[J].东北林业大学学报,2014,42(3):141-143.
[13]林燕青,吴承祯,谢安强,等.木麻黄(Casuarina equisetifolia)内生真菌的分离与促生菌株的筛选[J].北华大学学报(自然科学版),2015,16(4):522-528.
[14]张宪政.植物叶绿素含量测定:丙酮乙醇混合液法[J].辽宁农业科学,1986(3):28-30.
[15] SIEGEL M R,JOHNSON M C,VARNEY D R,et al. A fungal endophyte in tall fescue incidence and dissemination[J]. Phytopathol,1984,74(8):932-937.
[16] WAQAS M,KHAN A L,LEE I J. Bioactive chemical constituents produced by endophytes and effects on rice plant growth[J].Journal of Plant Interactions,2014,9(1):478-487.
[17] KHAN A L,AIHARRASI A,AIRAWAHI A,et al. Endophytic fungi from frankincense tree improves host growth and produces extracellular enzymes and indole acetic acid[J]. PLoS One,2016,11(6):e0158207.
[18] KHAN A L,LEE I J. Endophytic penicillium funiculosum LHL06 secretes gibberellin that reprograms Glycine max L. growth during copper stress[J]. BMC Plant Biology,2013,13(1):86.
[19] SIRRENBERG A,GOEBEL C,GROND S,et al. Piriformospora indica affects plant growth by auxin production[J]. Physiologia Plantarum,2007,131(4):581-589.
[20]吴静萍,钱吉,郑师章.兰花菌根菌分泌物成分的初步分析[J].应用生态学报,2002,13(7):845-848.
[21] BARAZANI O,DAHL C C,BALDWIN I T. Sebacina vermiferapromotes the growth and fitness of Nicotiana attenuataby inhibiting ethylene signaling[J]. Plant Physiology,2007,144(2):1223-1232.
[22] SHERAMTI I,SHAHOLLARI B,VENUS Y,et al. The endophytic fungus Piriformospora indica stimulates the expression of nitrate reductase and the starch-degrading enzyme glucan-water dikinase in tobacco and Arabidopsis roots through a homeodomain transcription factor that binds to a conserved motif in their promoters[J]. Journal of Biological Chemistry,2005,280(28):26241-26247.
[23] MALLA R,PRASAD R,KUMARI R,et al. Phosphorus solubilizing symbiotic fungus:Piriformospra indica[J]. Endocytobiosis Cell Research,2004,15(2):579-600.
[24] MARTINEZ D,LARRONDO L F,PUTNAM N,et al. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78[J]. Nature Biotechnology,2004,22(6):695-700.
[25]郭春爱,刘芳,许晓明.叶绿素b缺失与植物的光合作用[J].植物生理学通讯,2006,42(5):967-973.
[26]陈贝贝,王敏,胡莺雷,等.地黄内生真菌促生作用的初步研究[J].中国中药杂志,2011,36(5):1137-1140.
[27] WANG Q X,YAN N,JI D G. In vitro growth and carbon utilization of the green-leaved Orchid Dendrobium officinale are promoted by mycorrhical associations[J]. Botanical Studies,2013,54(1):1-9.
[28] CHEN J,ZHANG L C,XING Y M,et al. Diversity and taxonomy of endophytic xylariaceous fungi from medicinal plants of Dendrobium(Orchidaceae)[J]. PLoS One,2013,8(3). doi:10.1371/journal.pone.0058268.
[29] KRISTIANSEN K A,TAYLOR D L,KJOLLER R,et al. Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis(Orchidaceae)using single-trand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences[J]. Molecular Ecology,2001,10(8):2089-2093.
[2] GUO L D,HUANG G R,WANG Y. Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis(Pinaceae)in the Dong ling Mountains,Beijing[J]. Journal of Integrative Plant Biology,2008,50(8):997-1003.
[3] BREM D,LEUCHTMANN A. Intraspecific competition of endophyte infected vs uninfected plants oftwowoodland grass species[J]. Oikos,2002,96(2):281-290.
[4] CLAY K,SCHARDL C. Evolutionary origins and ecological consequences of endophyte symbiosis with grasses[J]. The American Naturalist,2002,160(S4):S99-S127.
[5]胡美玲,郑勇,孙翔,等.内生真菌促进玉米抗旱性研究[J].菌物学报,2017,36(11):1556-1565.
[6]张炜银,王伯荪,李鸣光,等.台湾相思林和芒草草丛中薇甘菊枝构件的分枝格局及其生物量[J].植物生态学报,2002,26(3):346-350.
[7]肖泽鑫,彭剑华,詹潮安,等.主成分分析在台湾相思优树选择标准和方法中的应用研究[J].中南林业科技大学学报,2012,32(5):54-58.
[8]周锦业,官国栋,林宇,等.东南沿海不同相思树种叶绿素荧光特性的季相变化[J].西南林业大学学报,2014,34(1):21-26.
[9]陈国华,陈海艳,李宝福,等.沿海沙地木麻黄更新迹地6种相思树的水源涵养功能比较[J].防护林科技,2012(2):1-4.
[10]吴晓菡,李文超,秦路平.天目山山胡椒不同部位内生真菌组成及多样性分析[J].植物资源与环境学报,2012(2):107-113.
[11]崔林,孙振,孙福在,等.马铃薯内生细菌的分离及环腐病拮抗菌的筛选鉴定[J].植物病理学报,2003,33(4):353-358.
[12]龚贺,张雷鸣,任伟超,等.膜荚黄芪内生真菌的分离与鉴定[J].东北林业大学学报,2014,42(3):141-143.
[13]林燕青,吴承祯,谢安强,等.木麻黄(Casuarina equisetifolia)内生真菌的分离与促生菌株的筛选[J].北华大学学报(自然科学版),2015,16(4):522-528.
[14]张宪政.植物叶绿素含量测定:丙酮乙醇混合液法[J].辽宁农业科学,1986(3):28-30.
[15] SIEGEL M R,JOHNSON M C,VARNEY D R,et al. A fungal endophyte in tall fescue incidence and dissemination[J]. Phytopathol,1984,74(8):932-937.
[16] WAQAS M,KHAN A L,LEE I J. Bioactive chemical constituents produced by endophytes and effects on rice plant growth[J].Journal of Plant Interactions,2014,9(1):478-487.
[17] KHAN A L,AIHARRASI A,AIRAWAHI A,et al. Endophytic fungi from frankincense tree improves host growth and produces extracellular enzymes and indole acetic acid[J]. PLoS One,2016,11(6):e0158207.
[18] KHAN A L,LEE I J. Endophytic penicillium funiculosum LHL06 secretes gibberellin that reprograms Glycine max L. growth during copper stress[J]. BMC Plant Biology,2013,13(1):86.
[19] SIRRENBERG A,GOEBEL C,GROND S,et al. Piriformospora indica affects plant growth by auxin production[J]. Physiologia Plantarum,2007,131(4):581-589.
[20]吴静萍,钱吉,郑师章.兰花菌根菌分泌物成分的初步分析[J].应用生态学报,2002,13(7):845-848.
[21] BARAZANI O,DAHL C C,BALDWIN I T. Sebacina vermiferapromotes the growth and fitness of Nicotiana attenuataby inhibiting ethylene signaling[J]. Plant Physiology,2007,144(2):1223-1232.
[22] SHERAMTI I,SHAHOLLARI B,VENUS Y,et al. The endophytic fungus Piriformospora indica stimulates the expression of nitrate reductase and the starch-degrading enzyme glucan-water dikinase in tobacco and Arabidopsis roots through a homeodomain transcription factor that binds to a conserved motif in their promoters[J]. Journal of Biological Chemistry,2005,280(28):26241-26247.
[23] MALLA R,PRASAD R,KUMARI R,et al. Phosphorus solubilizing symbiotic fungus:Piriformospra indica[J]. Endocytobiosis Cell Research,2004,15(2):579-600.
[24] MARTINEZ D,LARRONDO L F,PUTNAM N,et al. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78[J]. Nature Biotechnology,2004,22(6):695-700.
[25]郭春爱,刘芳,许晓明.叶绿素b缺失与植物的光合作用[J].植物生理学通讯,2006,42(5):967-973.
[26]陈贝贝,王敏,胡莺雷,等.地黄内生真菌促生作用的初步研究[J].中国中药杂志,2011,36(5):1137-1140.
[27] WANG Q X,YAN N,JI D G. In vitro growth and carbon utilization of the green-leaved Orchid Dendrobium officinale are promoted by mycorrhical associations[J]. Botanical Studies,2013,54(1):1-9.
[28] CHEN J,ZHANG L C,XING Y M,et al. Diversity and taxonomy of endophytic xylariaceous fungi from medicinal plants of Dendrobium(Orchidaceae)[J]. PLoS One,2013,8(3). doi:10.1371/journal.pone.0058268.
[29] KRISTIANSEN K A,TAYLOR D L,KJOLLER R,et al. Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis(Orchidaceae)using single-trand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences[J]. Molecular Ecology,2001,10(8):2089-2093.