“肾脑相济”电针疗法对围绝经期抑郁症模型大鼠海马及其内环境影响
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摘要
目的:研究以"肾脑相济"理论为指导的电针疗法对围绝经期抑郁症模型大鼠海马及其内环境的影响。方法:随机将40只雌性SD大鼠分成空白组、模型组、电针组和西药组,用去势和慢性不可预见性刺激相结合的方法建立围绝经期抑郁症大鼠模型,西药组予盐酸氯米帕明进行灌胃,电针组选取"百会""肾俞"和"三阴交"行电针刺激。观察比较各组大鼠糖水偏嗜和悬尾不动情况,ELISA法测定大鼠海马糖皮质激素受体(GR)、盐皮质激素受体(MR)的含量;尼氏染色法检测海马CA3区神经细胞数目及核仁数量、总面积、平均黑度、积分光密度。结果:与模型组相比,电针组和西药组糖水偏嗜率明显增加、悬尾不动时间显著缩短;GR和MR水平升高,CA3区神经细胞数目增多,胞内核仁数目、总面积、积分光密度、平均黑度均升高。结论:围绝经期抑郁状态下,海马内环境稳态被破坏,以"肾脑相济"理论为指导的电针疗法可以改善海马内环境,减少大鼠神经细胞的损伤,并为临床电针改善抑郁状态提供了有力依据。
Objective:To study the effect of electroacupuncture therapy on the hippocampus and its internal environment in the rat model of perimenopausal depression. Methods:40 SPF SD female rats were randomly divided into blank group,model group,EA group and clomipramine group. The rat models of perimenopausal depression were established by castration combined with chronic unpredictable stimulus. Medicine group was treated with clomipramine hydrochloride by intragastric administration,electro acupuncture group selected"Baihui"and"Shenshu"and"Sanyinjiao"for electroacupuncture. The behavior was measured by the sugar consumption experiments and the tail suspension test. ELISA method was used to determine the content of glucocorticoid receptor(GR)and mineralocorticoid receptor(MR)in hippocampus of rats. Nissl staining method was used to detect the number of nerve cells and nucleolus in hippocampal CA3 area. Results:Compared with model group,electroacupuncture group and Western medicine group,sucrose preference rate increased significantly,significantly shortened immobility time in tail suspension;increased GR and MR levels,CA3 nerve cell number increase,intracellular nucleolar number,total area,integral optical density and average density increased. Conclusion:In the perimenopausal depression state,the hippocampal homeostasis is destroyed. The electro acupuncture therapy guided by the theory of kidney and brain communication can improve the hippocampal environment and reduce the damage of nerve cells in rats,and provide a strong basis for clinical electroacupuncture to improve the state of depression.
Objective:To study the effect of electroacupuncture therapy on the hippocampus and its internal environment in the rat model of perimenopausal depression. Methods:40 SPF SD female rats were randomly divided into blank group,model group,EA group and clomipramine group. The rat models of perimenopausal depression were established by castration combined with chronic unpredictable stimulus. Medicine group was treated with clomipramine hydrochloride by intragastric administration,electro acupuncture group selected"Baihui"and"Shenshu"and"Sanyinjiao"for electroacupuncture. The behavior was measured by the sugar consumption experiments and the tail suspension test. ELISA method was used to determine the content of glucocorticoid receptor(GR)and mineralocorticoid receptor(MR)in hippocampus of rats. Nissl staining method was used to detect the number of nerve cells and nucleolus in hippocampal CA3 area. Results:Compared with model group,electroacupuncture group and Western medicine group,sucrose preference rate increased significantly,significantly shortened immobility time in tail suspension;increased GR and MR levels,CA3 nerve cell number increase,intracellular nucleolar number,total area,integral optical density and average density increased. Conclusion:In the perimenopausal depression state,the hippocampal homeostasis is destroyed. The electro acupuncture therapy guided by the theory of kidney and brain communication can improve the hippocampal environment and reduce the damage of nerve cells in rats,and provide a strong basis for clinical electroacupuncture to improve the state of depression.
引文
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[16]安磊,李静,张有志,等.糖皮质激素受体:抑郁症治疗的潜在靶标[J].军事医学,2014,38(11):908-911,917.
[17]张国双,杨坤.抗抑郁新靶点-糖皮质激素受体[J].国际精神病学杂志,2015(1):4.
[18]陈飞.糖皮质激素受体和盐皮质激素受体在抑郁症中的作用[J].医学美学美容,2015,5(1):627-627.
[19]杨秋佳,窦肇华,刘晓冬,等.大鼠发育中海马CA3区神经元形态、核及核仁的定量分析[J].中国老年学杂志,2003,23(2):105-107.
[20]侯小艳.孟安琪教授从肾脑失济论治围绝经期抑郁症[D].沈阳:辽宁中医药大学,2013.
[21]段莹,周佩,李君,等.针灸治疗围绝经期抑郁症研究进展[J].湖南中医杂志,2017,33(5):201-203.
[22]王戈.“肾脑相济”电针疗法对“天癸竭”围绝经期抑郁症作用的机制研究[D].沈阳:辽宁中医药大学,2016.
[2]Urbach A,Redecker C,Witte O W.Induction of neurogenesis in the adult dentate gyrus by cortical spreading depression[J].Stroke,2008,39(11):3064-3072.
[3]科技部.关于善待实验动物的指导性意见[M].北京:中华人民共和国科学技术部,2006:9-30.
[4]LI LH,WANG ZC,YU J,et al.Ovariectomy results in variable changes in nociception,mood and depression in adult female rats[J].Plo S One,2014,9(4):e94312.
[5]Willner P,Towell A,Sampson D,et al.Reduction of sucrose preference by chronic unpredictable mild stress,and its restoration by a tricyclic antidepressant[J].Psychopharmacology,1987,93(3):358-364.
[6]徐叔云,卞如濂,陈修.药理实验方法学[M].2版.北京:人民卫生出版社,1982:1535.
[7]李忠仁.实验针灸学[M].北京:中国中医中药出版社,2003:147-148.
[8]黄民,张征骄,唐笑迪.慢性可变应激对雌性大鼠动情周期及体质量和行为的影响[J].吉林医学,2008,29(17):1447-1448.
[9]张辉,宋思佳,师建国,等.昼夜节律,性别和品系对小鼠悬尾实验的影响[J].中华行为医学与脑科学杂志,2011,20(10):890-895.
[10]MO Y,YAO H,SONG H,et al.Alteration of behavioral changes and hippocampus galanin expression in chronic unpredictable mild stress-induced depression rats and effect of electroacupuncture treatment[J].Evidence-based Complementary and Alternative Medicine,2014,2014(2):179-796.
[11]汤艳清,谢光荣.抑郁症的脑结构影像学改变及其机制的研究进展[J].中国临床心理学杂志,2005,13(3):366-369.
[12]游伟,时宇静,韩焱晶,等.电针对慢性应激抑郁模型大鼠糖皮质激素及其受体基因表达的影响[J].针刺研究,2010,35(4):261-266.
[13]董瑞婕,吴爱勤.糖皮质激素受体在抑郁症病理机制中的地位[J].上海精神医学,2008,20(4):243.
[14]孙美芳.抑郁症模型大鼠糖皮质激素受体及海马形态的动态研究[D].镇江:江苏大学,2016:794.
[15]王姗姗.人体下丘脑室旁核多基因平衡紊乱与抑郁症[D].合肥:中国科学技术大学,2008.
[16]安磊,李静,张有志,等.糖皮质激素受体:抑郁症治疗的潜在靶标[J].军事医学,2014,38(11):908-911,917.
[17]张国双,杨坤.抗抑郁新靶点-糖皮质激素受体[J].国际精神病学杂志,2015(1):4.
[18]陈飞.糖皮质激素受体和盐皮质激素受体在抑郁症中的作用[J].医学美学美容,2015,5(1):627-627.
[19]杨秋佳,窦肇华,刘晓冬,等.大鼠发育中海马CA3区神经元形态、核及核仁的定量分析[J].中国老年学杂志,2003,23(2):105-107.
[20]侯小艳.孟安琪教授从肾脑失济论治围绝经期抑郁症[D].沈阳:辽宁中医药大学,2013.
[21]段莹,周佩,李君,等.针灸治疗围绝经期抑郁症研究进展[J].湖南中医杂志,2017,33(5):201-203.
[22]王戈.“肾脑相济”电针疗法对“天癸竭”围绝经期抑郁症作用的机制研究[D].沈阳:辽宁中医药大学,2016.