补肾健脑电针疗法对围绝经期抑郁症大鼠前额叶皮质神经细胞内尼氏小体及核仁影响的定量分析
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摘要
目的:观察补肾健脑电针疗法对围绝经期抑郁症大鼠前额叶皮质神经细胞病理形态改变,探讨基于"肾脑相济"理论为指导的电针疗法对此模型的治疗机制。方法:60只大鼠随机分为空白组、假手术组、模型组、西药组、电针组5组,采用去势和慢性不可预见刺激结合制备围绝经期抑郁症大鼠模型。连续治疗28 d,观察大鼠糖水实验及抓力实验,取大鼠脑半球固定,用尼氏染色,观察前额叶皮质神经细胞及尼氏小体形态结构,定量观察前额叶皮质区,通过计数神经细胞、测量神经细胞胞浆内尼氏小体面积、积分光密度、平均黑度及核仁数目、面积、积分光密度、平均黑度观察电针对围绝经期抑郁症模型大鼠前额叶皮质神经细胞病理形态改变的影响。结果:与空白组比较,造模后各组大鼠糖水偏嗜率及抓力有所下降(P<0.05)。治疗后,电针组及西药组与模型组比较糖水偏嗜率及抓力有所提高(P<0.05);电针组及西药组与假手术组比较糖水偏嗜率有所提高(P<0.05)。治疗后,电针组及西药组与模型组比较计数神经细胞、测量神经细胞胞浆内尼氏小体面积、积分光密度、平均黑度及核仁数目、面积、积分光密度、平均黑度均有所提高(P<0.05)。结论:补肾健脑电针疗法对围绝经期抑郁症大鼠前额叶皮质神经细胞尼氏小体及核仁状态产生影响,这可能是电针能够产生神经保护作用的基础。
Objective: To observe the effect of Bushen Jiannao acupuncture therapy of perimenopausal depression rats' prefrontal cortex nerve cells in pathological study,based on the kidney-brain theory guided mechanism of electro acupuncture therapy.Methods: Sixty rats were randomly divided into control group,sham group,model group,western medicine group and electro acupuncture group. We prepared perimenopausal depression rat model by OVX and CUMS. For 28 consecutive days,we observed the changes of sugar experiment and gripped the experimental results,the half of brain tissue and carried out Nissl staining. We observed cortex nerve cells and Nissl body structure,prefrontal cortex through the nerve cell count of nerve cells,measured pulp Nissl's body area,integral density and average number,density and nucleolus area,integral optical density and the average density index to investigate the effect of electroacupuncture on perimenopausal depression model changes in prefrontal cortex nerve cells in pathological big rat. Results: After treatment,compared with the control group,western medicine group and model group's partial addicted to sugar water rate and holding power increased(P < 0. 05). Electro acupuncture group and western medicine group' s sugar water partial heavy rate increased compared with that of the control group(P < 0. 05). After treatment,the curative group and western medicine group's count of nerve cells,nerve cells in the cytoplasm of nissl body area,integral density,average number of blackness and nucleoli,integral optical density and the average black degree increased compared with those of the model group(P < 0. 05). Conclusion: Bushen Jiannao electroacupuncture therapy has an effect on the Nissl body and nucleolus of the prefrontal cortex of the rats with perimenopausal depression.
Objective: To observe the effect of Bushen Jiannao acupuncture therapy of perimenopausal depression rats' prefrontal cortex nerve cells in pathological study,based on the kidney-brain theory guided mechanism of electro acupuncture therapy.Methods: Sixty rats were randomly divided into control group,sham group,model group,western medicine group and electro acupuncture group. We prepared perimenopausal depression rat model by OVX and CUMS. For 28 consecutive days,we observed the changes of sugar experiment and gripped the experimental results,the half of brain tissue and carried out Nissl staining. We observed cortex nerve cells and Nissl body structure,prefrontal cortex through the nerve cell count of nerve cells,measured pulp Nissl's body area,integral density and average number,density and nucleolus area,integral optical density and the average density index to investigate the effect of electroacupuncture on perimenopausal depression model changes in prefrontal cortex nerve cells in pathological big rat. Results: After treatment,compared with the control group,western medicine group and model group's partial addicted to sugar water rate and holding power increased(P < 0. 05). Electro acupuncture group and western medicine group' s sugar water partial heavy rate increased compared with that of the control group(P < 0. 05). After treatment,the curative group and western medicine group's count of nerve cells,nerve cells in the cytoplasm of nissl body area,integral density,average number of blackness and nucleoli,integral optical density and the average black degree increased compared with those of the model group(P < 0. 05). Conclusion: Bushen Jiannao electroacupuncture therapy has an effect on the Nissl body and nucleolus of the prefrontal cortex of the rats with perimenopausal depression.
引文
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[8]孙合亮,邱丽丽,贾敏,等氯胺酮对慢性应激抑郁大鼠行为学及海马一氧化氮合酶的影响[J].临床麻醉学杂志,2013,29(12):1207-1209.
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[11]吴迁,王思涵,马学红,等.电针抗抑郁临床疗效与中医症状改善的相关性研究[J].针灸临床杂志,2015,31(9):4-7.
[12]侯冬芬,罗和春.电针百会印堂治疗30例中风后抑郁患者临床疗效观察[J].中国针灸,1996,8(2):23-24.
[13]马学红,杨秀岩,许珂,等.针刺联合西药治疗轻中度抑郁症患者88例临床观察[J].中医杂志,2014,55(6):493-496.
[14]黄亮,孙忠人.电针治疗脑卒中后抑郁症的临床观察[J].针灸临床杂志,2005,21(5):48-49.
[15]赵珂,金季玲.金季玲教授治疗围绝经期抑郁症临床经验[J].四川中医,2011,29(8):13-14.
[16]周欣,薛文达,李守雪,等.不同抑郁症模型下证候差异的品系赖性研究[J].世界科学技术-中医药现代化,2016,18(2):151-156.
[17]Koenigs M.The functional neuroanatomy of depression:distinct roles for ventromedial and dorsolateral prefrontal cortex[J].Behavioural brain research,2009,201(2):239-243.
[18]Drevets WC,Price JL,Simpson JR,et al.Subgenual prefrontal cortex abnormalities in mood disorders[J].Nature,1997,386(6627):824-827.
[19]Lopez JP,Fiori LM,Gross JA,et al.Regulatory role of miRNAs in polyamine gene expression in the prefrontal cortex of depressed suicide completers[J].The international journal of neuropsychopharmacology/official scientific journal of the Collegium Internationale Neuropsychopharmacologicum(CINP),2014,17(1):23-32.
[20]Botteron KN,Raichle ME,Drevets WC,et al.Volumetric reduction in left subgenual prefrontal cortex in early onset depression[J].Biological psychiatry,2002,51(4):342-344.
[2]Li LH,Wang ZC,Yu J,et al.Ovariectomy results in variable changes in nociception,mood and depression in adult female rats[J].Plo S one,2014,9(4):e94312.
[3]Willner P,Towell A,Sampson D,et al.Reduction of sucrose preference by chronic unpredictable mild stress,and its restoration by a tricyclic antidepressant[J].Psychopharmacology,1987,93(3):358-364.
[4]Recamier-Carballo S,Estrada-Camarena E,Reyes R,et al.Synergistic effect of estradiol and fluoxetine in young adult and middleaged female rats in two models of experimental depression[J].Behavioural brain research,2012,233(2):351-358.
[5]徐叔云,卞如濂,陈修.药理实验方法学[M].2版.北京:人民卫生出版社,1982:1535.
[6]李忠仁.实验针灸学[M].北京:中国中医中药出版社,2003:147-148.
[7]王洁,吴素慧,尚海霞.性成熟雌性SD大鼠动情周期的观察[J].当代医学,2013,19(28):25-26.
[8]孙合亮,邱丽丽,贾敏,等氯胺酮对慢性应激抑郁大鼠行为学及海马一氧化氮合酶的影响[J].临床麻醉学杂志,2013,29(12):1207-1209.
[9]徐凯,陈秀玲,罗仁瀚.电针四神聪穴对抑郁症患者血清5-HT、DA含量影响的临床观察[J].新中医,2011,43(4):87-89.
[10]徐世芬,庄礼兴.电针百会、印堂为主治疗抑郁症疗效观察[J].上海针灸杂志,2010,29(9):569-572.
[11]吴迁,王思涵,马学红,等.电针抗抑郁临床疗效与中医症状改善的相关性研究[J].针灸临床杂志,2015,31(9):4-7.
[12]侯冬芬,罗和春.电针百会印堂治疗30例中风后抑郁患者临床疗效观察[J].中国针灸,1996,8(2):23-24.
[13]马学红,杨秀岩,许珂,等.针刺联合西药治疗轻中度抑郁症患者88例临床观察[J].中医杂志,2014,55(6):493-496.
[14]黄亮,孙忠人.电针治疗脑卒中后抑郁症的临床观察[J].针灸临床杂志,2005,21(5):48-49.
[15]赵珂,金季玲.金季玲教授治疗围绝经期抑郁症临床经验[J].四川中医,2011,29(8):13-14.
[16]周欣,薛文达,李守雪,等.不同抑郁症模型下证候差异的品系赖性研究[J].世界科学技术-中医药现代化,2016,18(2):151-156.
[17]Koenigs M.The functional neuroanatomy of depression:distinct roles for ventromedial and dorsolateral prefrontal cortex[J].Behavioural brain research,2009,201(2):239-243.
[18]Drevets WC,Price JL,Simpson JR,et al.Subgenual prefrontal cortex abnormalities in mood disorders[J].Nature,1997,386(6627):824-827.
[19]Lopez JP,Fiori LM,Gross JA,et al.Regulatory role of miRNAs in polyamine gene expression in the prefrontal cortex of depressed suicide completers[J].The international journal of neuropsychopharmacology/official scientific journal of the Collegium Internationale Neuropsychopharmacologicum(CINP),2014,17(1):23-32.
[20]Botteron KN,Raichle ME,Drevets WC,et al.Volumetric reduction in left subgenual prefrontal cortex in early onset depression[J].Biological psychiatry,2002,51(4):342-344.