GO/β-TCP复合生物陶瓷的细胞毒性研究
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摘要
将GO与β-TCP复合,通过无氧烧结工艺,形成0.1%GO复合β-TCP生物陶瓷。然后将该生物陶瓷与单相β-TCP、1%、0.1%、0.01%、0.001%GO悬浊液比较,将上述各材料的浸提液通过MTT法进行细胞毒性试验,并评价氧化石墨烯(GO)复合β-TCP生物陶瓷的细胞毒性的情况。结果表明0.1%GO复合β-TCP生物陶瓷与单相β-TCP生物陶瓷相比细胞相对增殖率无明显差异,GO悬浊液的细胞相对增殖率先随浓度降低逐渐增加,而在0.001%浓度条件下,细胞相对增殖率下降。以上各组结果均显示细胞毒性结果为0级,GO对β-TCP细胞毒性无影响。
An hybrid 0.1 wt% GO/β-TCP bioceramic is obtained by sintering in inert atmosphere a slurry of GO andβ-TCP. The cytotoxicity of this material is compared with those of pure β-TCP bioceramic, 1 wt%, 0.1 wt%, 0.01 wt% and 0.001 wt% GO suspension through MTT assay, and evaluate the cytotoxicity of graphene oxide(GO) composite β-TCP bioceramics. The results show No significant difference between hybrid GO/β-TCP bioceramic and pure β-TCP bioceramic was observed on relative growth rate(RGR). For GO suspension, a negative correlation was observed between cell proliferation and concentration in GO, but for low concentration(0.001 wt%), a decrease in RGR was noticed. For all of the tested groups, cytotoxicity level was found to be null. The addition of GO to β-TCP ceramics had no significant impact on the cytotoxicity of the material.
An hybrid 0.1 wt% GO/β-TCP bioceramic is obtained by sintering in inert atmosphere a slurry of GO andβ-TCP. The cytotoxicity of this material is compared with those of pure β-TCP bioceramic, 1 wt%, 0.1 wt%, 0.01 wt% and 0.001 wt% GO suspension through MTT assay, and evaluate the cytotoxicity of graphene oxide(GO) composite β-TCP bioceramics. The results show No significant difference between hybrid GO/β-TCP bioceramic and pure β-TCP bioceramic was observed on relative growth rate(RGR). For GO suspension, a negative correlation was observed between cell proliferation and concentration in GO, but for low concentration(0.001 wt%), a decrease in RGR was noticed. For all of the tested groups, cytotoxicity level was found to be null. The addition of GO to β-TCP ceramics had no significant impact on the cytotoxicity of the material.
引文
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[4]沈贺,张立明,张智军.石墨烯在生物医学领域的应用[J].东南大学学报(医学版),2011,30(1):218-223.
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[6]Zhang X,Yang R,Wang C,et al.Cell Biocompatibility of Functionalized Graphene Oxide[J].Acta Physico-Chimica Sinica,2012,28(6):1520-1524.
[7]徐宏杨,范敏敏,张志荣,等.氧化石墨烯PEG化后对L929细胞毒性的影响[J].华西药学杂志,2015,30(4):425-427.
[8]李建林,陈彬彬,章文,等.陶瓷/石墨烯块体复合材料的研究进展[J].无机材料学报,2014,29(3):225-236.
[9]帅词俊,彭淑平,高成德,等.利用石墨烯强韧化生物陶瓷材料及其人工骨的制备方法[P].中国:CN201210358185.4,2014-03-26.
[10]赵琰,毕见强,程福明,等.不同分散剂制备的石墨烯纳米片/双相磷酸钙生物复合材料及其力学性能研究[J].人工晶体学报,2013(5):927-932.
[11]刘奕,黄晶,李华.真空冷喷涂制备羟基磷灰石-石墨烯纳米复合生物涂层及其表界面特性研究.中国生物材料学会大会,2013.
[12]曾永香,贺瑞,裴锡波,等.电沉积制备氧化石墨烯-羟基磷灰石复合涂层[J].稀有金属材料与工程,2016(3):727-731.
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[14]Zhen Wang,Zheng Guo,Hua Bai,et al.Clinical Evaluation ofβ-TCP in the Treatment of Lacunar Bone Defects:A Prospective,Randomized Controlled Study[J].Materials Science&Engineering C Materials for Biological Applications,2013,33(4):1894-1899.
[15]强辉,王坤正,张明宇,等.β-磷酸三钙复合成骨细胞的异位成骨[J].中国组织工程研究,2009,13(12):2300-2304.
[16]史定伟,朱振安,戴尅戌,等.多孔磷酸三钙修复骨缺损的临床观察[J].国际骨科学杂志,2003,24(4):241-242.
[17]Jianxi Lu,Michel Descamps,Jacques Dejou,et al.The Biodegradation Mechanism of Calcium Phosphate Biomaterials in Bone[J].Journal of Biomedical Materials Research,2002,63(4):408-412.
[18]王彦伟,蔡舒,彭珍珍,等.多孔β-TCP生物材料的制备及其细胞毒性评价[J].稀有金属材料与工程,2005,34(2):1177-1180.
[19]关淑英,武峰,赵彬.MTT法检测氧化石墨烯的细胞毒性[J].中国现代医生,2014,52(17):18-20.
[20]赵琰.石墨烯/碳纳米管/双相磷酸钙生物陶瓷复合材料研究[D].济南:山东大学,2013.