一株锶高效富集真菌的筛选、鉴定及富集特性
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摘要
为利用微生物技术修复环境中的锶(Sr)污染,通过从锶污染的土壤中分离、筛选Sr的高效富集菌种,采用响应曲面法优化该菌对Sr的富集条件.结果显示,通过富集培养、稀释平板涂布筛选出一株对Sr具有较强富集能力的真菌,经鉴定该菌为Talaromyces sp..利用响应曲面法优化后,各因素的最佳水平组合为:培养温度35℃,p H值3.0,Sr初始质量浓度50 mg/L,该菌(干重)对Sr的富集量达到1 815.21 mg/kg,优化后的富集量与理论预测值较接近,说明响应曲面法建立的模型可用于预测该菌对Sr2+的富集特性.结果表明,筛选得到的Talaromyces sp.对Sr的富集能力强,可用于环境中的Sr污染修复.
In order to use microorganism removing environment of strontium,this paper obtained an efficient enrichment of fungi strain by screening of microorganisms from Sr2 +polluted soil. Meanwhile,Sr enrichment conditions of the fungi strain was optimized by using response surface method. The results showed a fungi strain has obtained,which has efficient enrichment ability for Sr,identifying as Talaromyces sp.. After the optimizing by response surface method,the best combination of various factors was: a culture temperature of 35℃,a p H value of 3 and a initial concentration of 50 mg / L,the enrichment value was up to 1815. 21 mg / kg,which was closed to the prediction. It showed that the model established from response surface method can be used to predict the fungus enrichment characteristics of Sr2 +. In general,Talaromyces sp. had stronger capacity of enriching Sr,it should have a great application potential to remove environment of strontium.
In order to use microorganism removing environment of strontium,this paper obtained an efficient enrichment of fungi strain by screening of microorganisms from Sr2 +polluted soil. Meanwhile,Sr enrichment conditions of the fungi strain was optimized by using response surface method. The results showed a fungi strain has obtained,which has efficient enrichment ability for Sr,identifying as Talaromyces sp.. After the optimizing by response surface method,the best combination of various factors was: a culture temperature of 35℃,a p H value of 3 and a initial concentration of 50 mg / L,the enrichment value was up to 1815. 21 mg / kg,which was closed to the prediction. It showed that the model established from response surface method can be used to predict the fungus enrichment characteristics of Sr2 +. In general,Talaromyces sp. had stronger capacity of enriching Sr,it should have a great application potential to remove environment of strontium.
引文
[1]TODOROV P T,ILIEVA E N.Contamination with uranium from natural and anthropological sources[J].Romanian J Physics,2006,51(1/2):27.
[2]唐世荣,商照荣,宋正国,等.放射性核素污染土壤修复标准的若干问题[J].农业环境科学学报,2007,6(2):407-412.
[3]NIELSEN S P.The biological role of strontium[J].Bone,2004,35(3):583-588.
[4]ACHAL V,PAN X L,ZHANG D Y.Bioremediation of strontium(Sr)contaminated aquifer quartz sand based on carbonate precipitation induced by Sr resistant Halomonas sp.[J].Chemosphere,2012,89(6):764-768.
[5]SINGH S,EAPEN S,THORA V,et al.Phytoremediation of 137 cesium and 90 strontium from solutions and low-level nuclear waste by Vetiveria zizanoides[J].Ecotoxicol Environ Safety,2007,69(2):306-311.
[6]MOYEN C,BONMORT J,ROBLIN G.Early Sr2+-induced effects on membrane potential,proton pumping-and ATP hydrolysis activities of plasma membrane vesicles from maize root cells[J].Environmental and Experimental Botany,2011,70(2/3):289-296.
[7]MOYEN C,ROBLIN G.Uptake and translocation of strontium in hydroponically grown maize plants,and subsequent effects on tissue ion content,growth and chlorophyll a/b ratio:comparison with Ca effects[J].Environmental and Experimental Botany,2010,68(3):247-257.
[8]SOUDEK P,VALENOVA S,VAVRIKOVA Z,et al.137Cs and90Sr uptake by sunflower cultivated under hydroponic conditions[J].J Environmental Radioactivity,2006,88(3):236-250.
[9]唐永金,罗学刚,曾峰,等.不同植物对高浓度Sr、Cs胁迫的响应与修复植物筛选[J].农业环境科学学报,2013,32(5):960-965.
[10]刘明学,董发勤,李姝,等.固定化耐辐射奇球菌对锶柱吸附与减量化研究[J].环境科学与技术,2014,37(6):32-37.
[11]杨玉山,董发勤,罗顺忠,等.固定化啤酒酵母对锶离子的吸附[J].化工进展,2012,31(3):639-642.
[12]李兆辉,王光明,徐云明,等.镉、汞、铅污染及其微生物修复研究进展[J].中国畜牧兽医,2010,37(9):39-43.
[13]肖湘竹,竹文坤,张友魁,等.微生物矿化法固结模拟放射性核素锶的研究[J].辐射防护,2015,35(2):65-70.
[14]中国环境监测总站.中国土壤元素背景值[M].北京:中国科学出版社,1990:87.
[15]LIU M X,DONG F Q,ZHANG W,et al.Biosorption of Uranium by Deinococcus radiodurans Cells under Culture Conditions[J].Advanced Materials Research,2012,535/536/537:2446-2449.
[16]刘明学.微生物与锶铀相互作用及其机理研究[D].成都:电子科技大学,2011.
[17]张伟,董发勤,代群威.啤酒酵母菌对溶液中锶离子的吸附行为[J].环境污染与防治,2009,31(8):11-15.
[18]邱亮,丰俊东.微生物对放射性核素吸附行为的研究进展[J].环境工程,2015,34(6):30-34.
[19]韩鹏飞,贺稚非,李洪军,等.微生物细胞壁结构及结合真菌毒素的研究进展[J].食品科学,2012,33(11):294-298.
[20]欧阳浩淼,金城.烟曲霉细胞壁及其GPI锚定结构研究进展[J].广西科学,2014,21(2):99-102.
[21]HERNANDEZ-ALLICA J,GARBISU C,BECERRIL J M,et al.Synthesis of low molecular weight thiols in response to Cd exposure in Thlaspi caerulescens[J].Plant Cell Environment,2006,29(7):1422-1429.
[22]KUPPER H,LOMBI E,ZHAO F J,et al.Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri[J].Planta,2000,212(1):75-84.
[23]刘明学,董发勤,李琼芳,等.酵母菌固定化及对锶吸附的影响[J].工业水处理,2009,5(5):19-23.
[2]唐世荣,商照荣,宋正国,等.放射性核素污染土壤修复标准的若干问题[J].农业环境科学学报,2007,6(2):407-412.
[3]NIELSEN S P.The biological role of strontium[J].Bone,2004,35(3):583-588.
[4]ACHAL V,PAN X L,ZHANG D Y.Bioremediation of strontium(Sr)contaminated aquifer quartz sand based on carbonate precipitation induced by Sr resistant Halomonas sp.[J].Chemosphere,2012,89(6):764-768.
[5]SINGH S,EAPEN S,THORA V,et al.Phytoremediation of 137 cesium and 90 strontium from solutions and low-level nuclear waste by Vetiveria zizanoides[J].Ecotoxicol Environ Safety,2007,69(2):306-311.
[6]MOYEN C,BONMORT J,ROBLIN G.Early Sr2+-induced effects on membrane potential,proton pumping-and ATP hydrolysis activities of plasma membrane vesicles from maize root cells[J].Environmental and Experimental Botany,2011,70(2/3):289-296.
[7]MOYEN C,ROBLIN G.Uptake and translocation of strontium in hydroponically grown maize plants,and subsequent effects on tissue ion content,growth and chlorophyll a/b ratio:comparison with Ca effects[J].Environmental and Experimental Botany,2010,68(3):247-257.
[8]SOUDEK P,VALENOVA S,VAVRIKOVA Z,et al.137Cs and90Sr uptake by sunflower cultivated under hydroponic conditions[J].J Environmental Radioactivity,2006,88(3):236-250.
[9]唐永金,罗学刚,曾峰,等.不同植物对高浓度Sr、Cs胁迫的响应与修复植物筛选[J].农业环境科学学报,2013,32(5):960-965.
[10]刘明学,董发勤,李姝,等.固定化耐辐射奇球菌对锶柱吸附与减量化研究[J].环境科学与技术,2014,37(6):32-37.
[11]杨玉山,董发勤,罗顺忠,等.固定化啤酒酵母对锶离子的吸附[J].化工进展,2012,31(3):639-642.
[12]李兆辉,王光明,徐云明,等.镉、汞、铅污染及其微生物修复研究进展[J].中国畜牧兽医,2010,37(9):39-43.
[13]肖湘竹,竹文坤,张友魁,等.微生物矿化法固结模拟放射性核素锶的研究[J].辐射防护,2015,35(2):65-70.
[14]中国环境监测总站.中国土壤元素背景值[M].北京:中国科学出版社,1990:87.
[15]LIU M X,DONG F Q,ZHANG W,et al.Biosorption of Uranium by Deinococcus radiodurans Cells under Culture Conditions[J].Advanced Materials Research,2012,535/536/537:2446-2449.
[16]刘明学.微生物与锶铀相互作用及其机理研究[D].成都:电子科技大学,2011.
[17]张伟,董发勤,代群威.啤酒酵母菌对溶液中锶离子的吸附行为[J].环境污染与防治,2009,31(8):11-15.
[18]邱亮,丰俊东.微生物对放射性核素吸附行为的研究进展[J].环境工程,2015,34(6):30-34.
[19]韩鹏飞,贺稚非,李洪军,等.微生物细胞壁结构及结合真菌毒素的研究进展[J].食品科学,2012,33(11):294-298.
[20]欧阳浩淼,金城.烟曲霉细胞壁及其GPI锚定结构研究进展[J].广西科学,2014,21(2):99-102.
[21]HERNANDEZ-ALLICA J,GARBISU C,BECERRIL J M,et al.Synthesis of low molecular weight thiols in response to Cd exposure in Thlaspi caerulescens[J].Plant Cell Environment,2006,29(7):1422-1429.
[22]KUPPER H,LOMBI E,ZHAO F J,et al.Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri[J].Planta,2000,212(1):75-84.
[23]刘明学,董发勤,李琼芳,等.酵母菌固定化及对锶吸附的影响[J].工业水处理,2009,5(5):19-23.