牛卵母细胞及着床前胚胎发育的蛋白质组学研究进展
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摘要
蛋白质组学作为一种能够全景式展示特定生物学过程的蛋白质表达谱的高通量研究手段,正被应用到越来越多的研究领域。牛的卵子发生以及着床前胚胎的生长发育都离不开蛋白质的一系列变化,通过蛋白质组学的研究手段可对牛卵母细胞成熟以及胚胎发育分子机制进行研究。前人通过构建成熟前后的卵母细胞或不同时期的着床前胚胎等的蛋白质组表达谱,来获得与卵母细胞成熟相关的标志蛋白,并对这些标志物进行验证和分析,将有利于理解牛卵母细胞成熟、体外受精及着床前胚胎发育的分子机制,为提高牛卵母细胞的成熟率以及胚胎的发育率及提高牛繁殖率等奠定基础。主要从双向聚丙烯酰胺凝胶电泳、色谱技术以及质谱技术等蛋白质组学研究手段为切入点,对蛋白质组学在牛卵母细胞和着床前胚胎发育过程中的研究进展进行综述,为进一步研究牛卵母细胞及附植前胚胎提供参考。
Proteomics,as a high-throughput method for displaying protein expression profiles of specific biological processes ina panoramic view,is being applied in more and more research fields. Bovine oogenesis and development of preimplantation embryo areinseparable from a series of changes in protein,thus the molecular mechanism of bovine oocyte maturation and embryo development can beinvestigated by proteomics. By establishing the proteomic expression profiles of oocytes before and after maturation as well as preimplantationembryos in different stages,we obtained marker proteins related to oocyte maturation and further validated and analyzed these markers,benefiting to understand the molecular mechanism of bovine oocyte maturation,in vitro fertilization and preimplantation embryo development. Itlays a foundation for improving the mature rate of bovine oocyte,the rate of embryonic development and the rate of bovine reproduction. In thispaper,the progress of proteomics in the bovine oocytes and development of preimplantation embryos was reviewed from the methods of studyingproteomics,such as two-dimensional polyacrylamide gel electrophoresis,chromatography and mass spectrometry. To provide reference forfurther study of bovine oocyte and preimplantation embrv.
Proteomics,as a high-throughput method for displaying protein expression profiles of specific biological processes ina panoramic view,is being applied in more and more research fields. Bovine oogenesis and development of preimplantation embryo areinseparable from a series of changes in protein,thus the molecular mechanism of bovine oocyte maturation and embryo development can beinvestigated by proteomics. By establishing the proteomic expression profiles of oocytes before and after maturation as well as preimplantationembryos in different stages,we obtained marker proteins related to oocyte maturation and further validated and analyzed these markers,benefiting to understand the molecular mechanism of bovine oocyte maturation,in vitro fertilization and preimplantation embryo development. Itlays a foundation for improving the mature rate of bovine oocyte,the rate of embryonic development and the rate of bovine reproduction. In thispaper,the progress of proteomics in the bovine oocytes and development of preimplantation embryos was reviewed from the methods of studyingproteomics,such as two-dimensional polyacrylamide gel electrophoresis,chromatography and mass spectrometry. To provide reference forfurther study of bovine oocyte and preimplantation embrv.
引文
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[26]Godovac-Zimmermann J,Brown LR.Perspectives for massspectrometry and functional proteomics[J].Mass SpectrometryReviews,2001,20(1):1-57.
[27]May C,Brosseron F,Chartowski P,et al.Instruments and methodsin proteomics[J].Methods in Molecular Biology,2011,696(696):3-26.
[28]Graves PR,Haystead TA.Molecular biologist’s guide to proteomics[J].Microbiology&Molecular Biology Reviews,2002,66(1):39.
[29]Sun H,Chen C,Lian B,et al.Identification of HPV integration andgene mutation in he La cell line by integrated analysis of RNA-Seqand MS/MS data[J].Journal of Proteome Research,2015,14(4):1678-1686.
[30]Chen C,Liu X,Zheng W,et al.Screening of missing proteins in thehuman liver proteome by improved MRM-approach-based targetedproteomics[J].Journal of Proteome Research,2014,13(4):1969-1978.
[31]Picotti P,Aebersold R.Selected reaction monitoringbased proteomics:workflows,potential,pitfalls and futuredirections[J].Nature Methods,2012,9(6):555-566.
[32]Coenen K,Massicotte L,Sirard MA.Study of newly synthesizedproteins during bovine oocyte maturation in vitro using imageanalysis of two-dimensional gel electrophoresis[J].Mol ReprodDev,2004,67(3):313-322.
[33]Massicotte L,Coenen K,Mourot M,et al.Maternal housekeepingproteins translated during bovine oocyte maturation and earlyembryo development[J].Proteomics,2006,6(13):3811-3820.
[34]Memili E,Peddinti D,Shack LA,et al.Bovine germinal vesicleoocyte and cumulus cell proteomics[J].Reproduction,2007,133(6):1107-1120.
[35]Peddinti D,Memili E,Burgess SC.Proteomics-based systemsbiology modeling of bovine germinal vesicle stage oocyte andcumulus cell interaction[J].PLo S One,2010,5(6)e11240.
[36]刘振方,付强,蒋丽,等.水牛卵母细胞成熟前后差异蛋白质组的初步研究[C].中国畜牧兽医学会动物繁殖学分会第十六届学术研讨会论文集.哈尔滨:中国畜牧兽医学会,2012.
[37]陈富美,付强,黄德伦,等.基于LC-MS/MS策略建立水牛卵母细胞蛋白质表达谱的方法研究[J].基因组学与应用生物学.2015(5):967-971.
[38]陈凌声,代小丽,徐永茹,等.成熟前后水牛卵母细胞蛋白质组的初步研究[J].畜牧兽医学报,2016,17(10):2003-2011.
[39]Chen L,Zhai L,Qu C,et al.Comparative proteomic analysis ofbuffalo oocytes matured in vitro using i TRAQ technique[J].Scientific Reports.2016,6:31795.
[40]Frei RE,Schultz GA,Church RB.Qualitative and quantitativechanges in protein synthesis occur at the 8-16-cell stage ofembryogenesis in the cow[J].Journal of reproduction andfertility,1989,86(2):637-641.
[41]Demant M.Qualitative and quantitative proteome analyses ofbovine oocytes and early embryos[D].Münchenr:LudwigMaximilians-Universit?t,2012.
[42]Virant-Klun I,Leicht S,Hughes C,et al.Identification ofMaturation-Specific Proteins by Single-Cell Proteomics of HumanOocytes[J].Molecular&Cellular Proteomics,2016,15(8):2616-2627.
[2]Memili E,Peddinti D,Shack L A,et al.Bovine germinal vesicleoocyte and cumulus cell proteomics[J].Reproduction,2007,133(6):1107-1120.
[3]Sirard MA,Richard F,Mayes M.Controlling meiotic resumption inbovine oocytes:a review[J].Theriogenology,1998,49(2):483-497.
[4]Motlík J,Pavlok A,Lapathitis G,et al.Impact of two-step invitro culture systems on developmental potency of oocytes[J].Reproduction in Domestic Animals,2000,35(6):267-271.
[5]Ellederova Z,Halada P,Man P,et al.Protein patterns of pig oocytesduring in vitro maturation1[J].Biology of Reproduction,2004,71(5):1533.
[6]Sun QY,Nagai T.Molecular mechanisms underlying pig oocytematuration and fertilization[J].Journal of Reproduction&Development,2003,49(5):347.
[7]Li L,Zheng P,Dean J.Maternal control of early mousedevelopment[J].Development,2010,137(6):859.
[8]Gygi SP,Rochon Y,Franza BR,et al.Correlation between proteinand m RNA abundance in yeast[J].Molecular&Cellular Biology,1999,19(3):1720-1730.
[9]Wilkins MR,Sanchez JC,Gooley AA,et al.Progress with proteomeprojects:why all proteins expressed by a genome should beidentified and how to do it[J].Biotechnology&GeneticEngineering Reviews,1996,13(1):19-50.
[10]Service RF.High-speed biologists search for gold in proteins[J].Science,2001,294(294):2074-2077.
[11]Rao VS,Srinivas K,Sujini GN,et al.Protein-protein interactiondetection:methods and analysis[J].International Journal ofProteomics,2013,2014(4):147648.
[12]Tyers M,Mann M.From genomics to proteomics[J].Nature,2003,422(6928):193.
[13]Hendrickson RC,Douglass JF,Reynolds LD,et al.Massspectometric identification of Mtb81,a novel serological marker fortuberculosis[J].Journal of Clinical Microbiology,2000,38(6):2354-2361.
[14]高昭辉,董书伟,薛慧文,等.纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析[J].中国畜牧兽医,2012,39(11):22-26.
[15]Fey SJ,Larsen PM.2D or not 2D.Two-dimensional gelelectrophoresis[J].Current Opinion in Chemical Biology,2001,5(1):26-33.
[16]Klein E,Klein JB,Thongboonkerd V.Two-dimensional gelelectrophoresis:a fundamental tool for expression proteomicsstudies[J].Contributions to Nephrology,2004,141(2):25.
[17]Chang J,Van RH,Ward WF,et al.Processing of data generated by2-dimensional gel electrophoresis for statistical analysis:missingdata,normalization,and statistics[J].Journal of ProteomeResearch,2004,3(6):1210-1218.
[18]Vogeser M.Liquid chromatography-tandem mass spectrometry--application in the clinical laboratory[M].Springer-Verlag,2003:117-126.
[19]Smyth WF,Brooks P.A critical evaluation of high performanceliquid chromatography-electrospray ionisation-mass spectrometryand capillary electrophoresis-electrospray-mass spectrometry forthe detection and determination of small molecules of significancein clinical and forensic science[J].Electrophoresis,2004,25(10-11):1413-1446.
[20]Takahashi N,Kaji H,Yanagida M,et al.Proteomics:advancedtechnology for the analysis of cellular function[J].Journal ofNutrition,2003,133(6 Suppl 1):2090S.
[21]Cutillas P,Burlingame A,Unwin R.Proteomic strategies and theirapplication in studies of renal function[J].News Physiol Sci,2004,19(2):114-119.
[22]Domon B,Aebersold R.Mass spectrometry and proteinanalysis[J].Science,2006,312(5771):212-217.
[23]Cho A,Normile D.Nobel prize in chemistry.Mastering macromolecules[J].Science,2002,298(5593):527-528.
[24]陈维斌.质谱技术原理与基础[C].生物技术药物理化特性分析与质量研究技术研讨会论文集.北京:中国药学会,2015.
[25]Andersen JS,Mann M.Functional genomics by mass spectrometryFEBS Letters[J].Febs Letters,2000,480(1):25.
[26]Godovac-Zimmermann J,Brown LR.Perspectives for massspectrometry and functional proteomics[J].Mass SpectrometryReviews,2001,20(1):1-57.
[27]May C,Brosseron F,Chartowski P,et al.Instruments and methodsin proteomics[J].Methods in Molecular Biology,2011,696(696):3-26.
[28]Graves PR,Haystead TA.Molecular biologist’s guide to proteomics[J].Microbiology&Molecular Biology Reviews,2002,66(1):39.
[29]Sun H,Chen C,Lian B,et al.Identification of HPV integration andgene mutation in he La cell line by integrated analysis of RNA-Seqand MS/MS data[J].Journal of Proteome Research,2015,14(4):1678-1686.
[30]Chen C,Liu X,Zheng W,et al.Screening of missing proteins in thehuman liver proteome by improved MRM-approach-based targetedproteomics[J].Journal of Proteome Research,2014,13(4):1969-1978.
[31]Picotti P,Aebersold R.Selected reaction monitoringbased proteomics:workflows,potential,pitfalls and futuredirections[J].Nature Methods,2012,9(6):555-566.
[32]Coenen K,Massicotte L,Sirard MA.Study of newly synthesizedproteins during bovine oocyte maturation in vitro using imageanalysis of two-dimensional gel electrophoresis[J].Mol ReprodDev,2004,67(3):313-322.
[33]Massicotte L,Coenen K,Mourot M,et al.Maternal housekeepingproteins translated during bovine oocyte maturation and earlyembryo development[J].Proteomics,2006,6(13):3811-3820.
[34]Memili E,Peddinti D,Shack LA,et al.Bovine germinal vesicleoocyte and cumulus cell proteomics[J].Reproduction,2007,133(6):1107-1120.
[35]Peddinti D,Memili E,Burgess SC.Proteomics-based systemsbiology modeling of bovine germinal vesicle stage oocyte andcumulus cell interaction[J].PLo S One,2010,5(6)e11240.
[36]刘振方,付强,蒋丽,等.水牛卵母细胞成熟前后差异蛋白质组的初步研究[C].中国畜牧兽医学会动物繁殖学分会第十六届学术研讨会论文集.哈尔滨:中国畜牧兽医学会,2012.
[37]陈富美,付强,黄德伦,等.基于LC-MS/MS策略建立水牛卵母细胞蛋白质表达谱的方法研究[J].基因组学与应用生物学.2015(5):967-971.
[38]陈凌声,代小丽,徐永茹,等.成熟前后水牛卵母细胞蛋白质组的初步研究[J].畜牧兽医学报,2016,17(10):2003-2011.
[39]Chen L,Zhai L,Qu C,et al.Comparative proteomic analysis ofbuffalo oocytes matured in vitro using i TRAQ technique[J].Scientific Reports.2016,6:31795.
[40]Frei RE,Schultz GA,Church RB.Qualitative and quantitativechanges in protein synthesis occur at the 8-16-cell stage ofembryogenesis in the cow[J].Journal of reproduction andfertility,1989,86(2):637-641.
[41]Demant M.Qualitative and quantitative proteome analyses ofbovine oocytes and early embryos[D].Münchenr:LudwigMaximilians-Universit?t,2012.
[42]Virant-Klun I,Leicht S,Hughes C,et al.Identification ofMaturation-Specific Proteins by Single-Cell Proteomics of HumanOocytes[J].Molecular&Cellular Proteomics,2016,15(8):2616-2627.