香水莲花提取物对胰脂肪酶活性的抑制作用
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摘要
采用96微孔板法测定香水莲花整花、雄蕊、花瓣、花托醇提物以及雄蕊不同极性部位提取物对胰脂肪酶的抑制活性;通过酶反应动力学方法确定雄蕊乙酸乙酯提取物对胰脂肪酶的抑制类型,并考察其在人工模拟消化肠液中对胰脂肪酶抑制效果的影响。结果表明,香水莲花各部位的醇提物对胰脂肪酶都具有一定的抑制作用,雄蕊醇提物的抑制作用最强。雄蕊不同极性部位提取物对胰脂肪酶的抑制活性由强到弱依次是乙酸乙酯提取物(半数抑制浓度IC_(50)=5.64mg/mL)>二氯甲烷提取物(IC_(50)=8.75 mg/mL)>正己烷提取物(IC_(50)=11.80 mg/mL)>正丁醇提取物(IC_(50)=22.47 mg/mL)。乙酸乙酯提取物对胰脂肪酶的作用类型为非竞争性抑制;经体外模拟消化后,对胰脂肪酶仍有较强的抑制效果,而且抑制率随浓度的升高而增大。因此,香水莲花提取物是一种具有胰脂肪酶抑制活性的优良植物源原料,可用于降脂功能性产品的开发。
Inhibitory activity of ethanol extracts from Nymphaea hybrid flowers containing whole flowers, stamens, petals and tori as well as different polar extracts from stamens was determined by 96 microporosity plates. The inhibition type of ethyl acetate extracts was confirmed by enzymatic kinetics assay. After simulated digesting by artificial intestine fluid, the inhibitory effect of ethyl acetate extracts on pancreatic lipase was measured. The results indicated that each ethanol extracts performed inhibition activity while the stamens extract showed the best. The order sequenced by inhibitory ability on pancreatic lipase from strong to weak was: ethyl acetate extract(IC_(50)=5.64 mg/mL)>methylene chloride extract(IC_(50)=8.75 mg/mL)>hexane extract(IC_(50)=11.80 mg/mL)>butanol extract(IC_(50)=22.47 mg/mL). Moreover, the inhibition type of ethyl acetate extracts was non-competitive inhibition. In addition, the digested ethyl acetate extracts still exhibited strong lipase inhibitory activity and its inhibitory rate increased with the rising of concentration. Thus, extracts of Nymphaea hybrid flowers could be used as good plant-originated raw materials with inhibitory effect on pancreatic lipase for the development of lipidlowering functional products.
Inhibitory activity of ethanol extracts from Nymphaea hybrid flowers containing whole flowers, stamens, petals and tori as well as different polar extracts from stamens was determined by 96 microporosity plates. The inhibition type of ethyl acetate extracts was confirmed by enzymatic kinetics assay. After simulated digesting by artificial intestine fluid, the inhibitory effect of ethyl acetate extracts on pancreatic lipase was measured. The results indicated that each ethanol extracts performed inhibition activity while the stamens extract showed the best. The order sequenced by inhibitory ability on pancreatic lipase from strong to weak was: ethyl acetate extract(IC_(50)=5.64 mg/mL)>methylene chloride extract(IC_(50)=8.75 mg/mL)>hexane extract(IC_(50)=11.80 mg/mL)>butanol extract(IC_(50)=22.47 mg/mL). Moreover, the inhibition type of ethyl acetate extracts was non-competitive inhibition. In addition, the digested ethyl acetate extracts still exhibited strong lipase inhibitory activity and its inhibitory rate increased with the rising of concentration. Thus, extracts of Nymphaea hybrid flowers could be used as good plant-originated raw materials with inhibitory effect on pancreatic lipase for the development of lipidlowering functional products.
引文
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[19]姜洪芳,石宝俊,赵伯涛,等.高速逆流色谱法(HSCCC)分离制备香水莲花中的柚皮素[J].中国野生植物资源,2012,31(6):40-43
[20]LUNAGARIYA NA,PATEL NK,JAGTAP SC,et al.Inhibitors of pancreatic lipase:state of the art and clinical perspectives[J].Journal of Experimental and Clinical Sciences,2014,13:897
[2]李强,任虹,王友升.植物源脂肪酶抑制剂研究进展[J].天然产物研究与开发,2015,27(02):360-366
[3]GARZA A L,ETXEBERRIA U,LOSTAO M P,et al.Helichrysum and grape fruit extracts inhibit carbohydrate digestion and absorption,improving postprandial glucose levels and hyperinsulinemia in rats[J].Journal of Agricultural Food Chemistry,2013,61(49):12012-12019
[4]CHOI B K,PARK S B,LEE D R,et al.Green coffee bean extract improves obesity by decreasing body fat in highfat diet-induced obese mice[J].Asian Pacific Journal of Tropical Medicine,2016,9(7):635-643
[5]任红荣,姜洪芳,单承莺,等.微波提取香水莲花总黄酮的工艺研究[J].中国野生植物资源,2010,29(2):30-33
[6]吕婷.利用模式生物秀丽隐杆线虫对植物提取物抗衰老的研究[D].南京:南京师范大学,2014
[7]PODSEDEK A,MAJEWSKA I,REDZYNIA M,et al.In vitro inhibitory effect on digestive enzymes and antioxidant potential of commonly consumed fruits[J].Journal of Agricultural Food Chemistry,2014,62(20):4610-4617
[8]ESPINAL-RUIZ M,RESTREPO-SáNCHEZ L,NARVáEZ-CUENCA C,et al.Impact of pectin properties on lipid digestion under simulated gastrointestinal conditions:Comparison of citrus and banana passion fruit(Passiflora tripartita var.mollissima)pectins[J].Food Hydrocolloids,2016,52:329-342
[9]刘天囡,徐梦佳,胡冰,等.茯砖茶多酚类物质对胰脂肪酶活性的抑制作用[J].食品科学,2015,36(21):46-49
[10]You Q,Chen F,Wang X,et al.Inhibitory effects of muscadine anthocyanins on alpha-glucosidase and pancreatic lipase activities[J].Journal of Agricultural Food Chemistry,2011,59(17):9506-9511
[11]赵军,徐芳,吉腾飞,等.睡莲属植物化学成分及生物活性研究进展[J].天然产物研究与开发,2014,26(1):142-147
[12]金敬红,张卫明.香水莲花的食用安全性研究[J].中国野生植物资源,2015,34(1):5-9
[13]张卫明,姜洪芳,徐辉,等.香水莲花降血脂作用的实验研究[J].食品工业科技,2009,30(2):111-112
[14]任秀娟,马海乐.葡萄籽提取物脂肪酶抑制活性筛选及抑制机制的研究[J].食品工业科技,2009,30(6):181-183
[15]顾君,孙怡.侧柏叶不同提取部位抑制胰脂肪酶及抗氧化活性筛选[J].中国实验方剂学杂志,2015,21(14):141-144
[16]杨龙佳,王进,杨庆雄.红茶提取物对胰脂肪酶抑制活性的研究[J].食品科技,2015,40(8):212-216
[17]RAHIM ATMA,TAKAHASHI Y,YAMAKI K.Mode of pancreatic lipase inhibition activity in vitro by some flavonoids and non-flavonoid polyphenols[J].Food Research International,2015,75:289-294
[18]YUDA N,TANAKA M,SUZUKI M,et al.Polyphenols extracted from black tea(Camellia sinensis)residue by hot-compressed water and their inhibitory effect on pancreatic lipase in vitro[J].Journal of Food Science,2012,77(12):H254-H261
[19]姜洪芳,石宝俊,赵伯涛,等.高速逆流色谱法(HSCCC)分离制备香水莲花中的柚皮素[J].中国野生植物资源,2012,31(6):40-43
[20]LUNAGARIYA NA,PATEL NK,JAGTAP SC,et al.Inhibitors of pancreatic lipase:state of the art and clinical perspectives[J].Journal of Experimental and Clinical Sciences,2014,13:897