大豆荚数垂直分布的遗传分析与QTL定位
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摘要
大豆荚数类型和空间分布决定产量的形成。以有一个共同亲本的两个重组自交系群体为遗传材料,按照植株的上、中、下3个部位调查一粒荚、二粒荚、三粒荚和四粒荚的数量,采用完备复合区间作图法进行QTL定位。结果表明:在两个群体中一粒荚、二粒荚、三粒荚、四粒荚在空间分布存在极显著的变异,部位间不同豆荚类型组成有显著差异,并且两个群体不同类型豆荚在不同部位分布的遗传基础不同,共检测到59个控制荚数性状的QTL,解释了0～11.45%的表型变异;两个群体检测到的荚数QTL中有30个QTL是本研究首次发现的。
The type of pod containing one, two, three and four seeds and vertical distribution of number decided the formation of yield in soybean. In this research two recombinant inbred lines populations with one common parent were used to detect QTL by combining a SSR linkage map and the phenotypic data of number one-, two-, three-and four-seed pod in upper, middle and lower part of main stem through inclusive compositive interval mapping method. The result showed that there were significant variations for the number one-, two-, three-and four-seed pods among upper, middle and lower part and the genetic basement controlling vertical distribution of pod number between two populations was different. There were 59 QTL controlling pod number with PVE of 0-11.45% in two populations. Among all QTLs, 30 were novelly discoveried.
The type of pod containing one, two, three and four seeds and vertical distribution of number decided the formation of yield in soybean. In this research two recombinant inbred lines populations with one common parent were used to detect QTL by combining a SSR linkage map and the phenotypic data of number one-, two-, three-and four-seed pod in upper, middle and lower part of main stem through inclusive compositive interval mapping method. The result showed that there were significant variations for the number one-, two-, three-and four-seed pods among upper, middle and lower part and the genetic basement controlling vertical distribution of pod number between two populations was different. There were 59 QTL controlling pod number with PVE of 0-11.45% in two populations. Among all QTLs, 30 were novelly discoveried.
引文
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[18] Yosuke K,Akito K,Norihiko T,et al.QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields[J].Ecology & Evolution,2013,3(7):2150-2168.
[19] Yang Z,Xin D,Liu C,et al.Erratum to:Identification of QTLs for seed and pod traits in soybean and analysis for additive effects and epistatic effects of QTLs among multiple environments[J].Molecular Genetics and Genomics,2013,288(12):651.
[20] Li D M,Sun M M,Han Y P,et al.Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold (Sclerotinia sclerotiorum)[J].Euphytica,2010,172(1):49-57.
[21] 王建康.数量性状基因的完备区间作图方法[J].作物学报,2009,35(2):239-245.(Wang J K.Inclusive composite interval mapping of quantitative trait genes[J].Acta Agrinomica Sinica,2009,35(2):239-245.)
[22] Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109(1):122-128.
[2] Liu Y P,Li R P,Li Z G.Effects of cultivation pattern and density on canopy structure and yield of soybean.[J].Soybean Science,2010,29(5):796-795.
[3] Rodrigues J I D S,Miranda F D D,Piovesan N D,et al.QTL mapping for yield components and agronomic traits in a Brazilian soybean population[J].Crop Breeding and Applied Biotechnology,2016,16(4):265-273.
[4] Liu N,Li M,Hu X,et al.Construction of high-density genetic map and QTL mapping of yield-related and two quality traits in soybean RILs population by RAD-sequencing[J].BMC Genomics,2017,18(1):466.
[5] Zhao G,Wang J,Han Y,et al.Identification of QTL underlying the resistance of soybean to pod borer,Leguminivora glycinivorella,(Mats.) obraztsov,and correlations with plant,pod and seed traits[J].Euphytica,2008,164(1):275-282.
[6] 王贤智,张晓娟,周蓉,等.大豆重组自交系群体荚粒性状的QTL分析[J].作物学报,2007,33(3):441-448.(Wang X Z,Zhang X J,Zhou R,et al.QTL analysis of seed and pod traits in soybean RIL population[J].The Crop Journal,2007,33(3):441-448.
[7] 朱晓丽.大豆遗传图谱构建及在两个群体重要农艺性状的QTL定位[D].哈尔滨:东北农业大学,2006.(Zhu X L.Construction of genetic linkage map and QTL mapping of important agronomic traits in two soybean populations[D].Harbin:Northeast Agricultural University,2016.)
[8] 李灿东,蒋洪蔚,张闻博,等.大豆荚粒相关性状的QTL分析[J].分子植物育种,2008,6(6):1091-1100.(Li C D,Jiang H W,Zhang W B,et al.QTL analysis of seed and pod traits in soybean[J].Molecular Plant Breeding,2008,6(6):1091-1100.)
[9] 吴晓雷,王永军,贺超英,等.大豆重要农艺性状的QTL分析[J].遗传学报,2001,46(10):947-955.(Wu X L,Wang Y J,He C Y,et al.QTL analysis of important agronomic traits in soybean[J].Journal of Genetics and Genomics,2001,46(10):947-955.)
[10] 周蓉,陈海峰,王贤智,等.大豆产量和产量构成因子及倒伏性的QTL分析[J].作物学报,2009,35(5):821-830.(Zhou R,Chen H F,Wang X Z,et al.QTL analysis of yield,yield components and lodging in soybean[J].Acta Agronomica Sinica,2009,35(5):821-830.)
[11] Zhang D,Cheng H,Wang H,et al.Identification of genomic regions determining flower and pod numbers development in soybean (Glycine max L.)[J].Journal of Genetics and Genomics,2010,37(8):545-556.
[12] Chen Q,Zhang Z,Liu C,et al.QTL analysis of major agronomic traits in soybean[J].Agricultural Sciences in China,2007,6(4):399-405.
[13] Sun D,Li W,Zhang Z,et al.Quantitative trait loci analysis for the developmental behavior of soybean (Glycine max L.Merr.)[J].Theoretical and Applied Genetics,2006,112(4):665-673.
[14] Vieira A J D,Oliveira D A,Soares T C B,et al.Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7 and F8 generations[J].Brazilian Journal of Plant Physiology,2006,18(2):281-290.
[15] Liu W,Kim M Y,van K,et al.QTL identification of yield-related traits and their association with flowering and maturity in soybean[J].Journal of Crop Science and Biotechnology,2011,14(1):65-70.
[16] Bobby R,Bazelle R,Clark W,et al.Genetic analysis of yield components in the PI 438489B by hamilton recombinant inbred line (RIL) population of soybean [Glycine max (L.) Merr.][J].Journal of Agricultural Science,2012,4(9):98.
[17] Palomeque L,Liu L J,Li W,et al.QTL in mega-environments:II.Agronomic trait QTL co-localized with seed yield QTL detected in a population derived from a cross of high-yielding adapted×high-yielding exotic soybean lines[J].Theoretical and Applied Genetics,2009,119(3):429-436.
[18] Yosuke K,Akito K,Norihiko T,et al.QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields[J].Ecology & Evolution,2013,3(7):2150-2168.
[19] Yang Z,Xin D,Liu C,et al.Erratum to:Identification of QTLs for seed and pod traits in soybean and analysis for additive effects and epistatic effects of QTLs among multiple environments[J].Molecular Genetics and Genomics,2013,288(12):651.
[20] Li D M,Sun M M,Han Y P,et al.Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold (Sclerotinia sclerotiorum)[J].Euphytica,2010,172(1):49-57.
[21] 王建康.数量性状基因的完备区间作图方法[J].作物学报,2009,35(2):239-245.(Wang J K.Inclusive composite interval mapping of quantitative trait genes[J].Acta Agrinomica Sinica,2009,35(2):239-245.)
[22] Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109(1):122-128.