苦参与抗生素配伍后对金黄色葡萄球菌抑菌效果的比较
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摘要
为了探讨常用抗生素与苦参配伍后联合抑菌效果。以苦参、青霉素、头孢拉定、左氧氟沙星、磷霉素钠为试验药物,以金黄色葡萄球菌为试验菌种,采用倍比稀释法检测五种药物的最小抑菌浓度,棋盘稀释法检测苦参与青霉素、头孢拉定、左氧氟沙星、磷霉素钠四种抗生素联用对金黄色葡萄球菌的联合抑菌浓度。结果表明,苦参、青霉素、头孢拉定、左氧氟沙星、磷霉素钠最小抑菌浓度分别为160mg/mL、0. 25μg/mL、1μg/mL、0. 5μg/mL、0. 5μg/mL;苦参与青霉素联用后表现为协同作用;苦参与头孢拉定联用后表现为相加作用;苦参与磷霉素钠、左氧氟沙星联用后表现为无关作用。表明苦参与青霉素和头孢拉定联用可达到高效抑制金黄色葡萄球菌的作用,对苦参兽医临床应用具有参考意义。
To investigate the bacteriostatic effect of common antibiotics combined withRadix Sophorae Flavescentisin vitro. The Radix Sophorae Flavescentis,Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium were selected as investigational bacterium,and the Staphylococcus Aureus was selected as investigated bacterium,and then we used the method of double dilution to detect the minimal inhibitory concentration of five drugs,and the fractional inhibitory concentration of Radix Sophorae Flavescentis was detected by chessboard dilution method in alliance combining with Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium. The results showed thatthe minimum inhibitory concentrations of Radix Sophorae Flavescentis,Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium were 160 mg/mL,0. 25 μg/mL,1 μg/mL,0. 5 μg/mL,0. 5 μg/mL,respectively. The fractional inhibitory concentration of Radix Sophorae Flavescentis showed the synergistic action combine with Penicillin,and additive action combine with Cefradine,and indifference action combine with Levofloxacin or Fosfomycin Sodium. The combination of Radix Sophorae Flavescentis and Penicillin or Cefradine can effectively inhibit the Staphylococcus Aureus which has a reference for the veterinaryclinical application.
To investigate the bacteriostatic effect of common antibiotics combined withRadix Sophorae Flavescentisin vitro. The Radix Sophorae Flavescentis,Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium were selected as investigational bacterium,and the Staphylococcus Aureus was selected as investigated bacterium,and then we used the method of double dilution to detect the minimal inhibitory concentration of five drugs,and the fractional inhibitory concentration of Radix Sophorae Flavescentis was detected by chessboard dilution method in alliance combining with Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium. The results showed thatthe minimum inhibitory concentrations of Radix Sophorae Flavescentis,Penicillin,Cefradine,Levofloxacin and Fosfomycin Sodium were 160 mg/mL,0. 25 μg/mL,1 μg/mL,0. 5 μg/mL,0. 5 μg/mL,respectively. The fractional inhibitory concentration of Radix Sophorae Flavescentis showed the synergistic action combine with Penicillin,and additive action combine with Cefradine,and indifference action combine with Levofloxacin or Fosfomycin Sodium. The combination of Radix Sophorae Flavescentis and Penicillin or Cefradine can effectively inhibit the Staphylococcus Aureus which has a reference for the veterinaryclinical application.
引文
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[2]柴同杰,赵云玲,刘文波,等.鸡舍环境耐药细菌气溶胶及其向环境传播的研究[J].中国预防兽医学报,2003,25(3):209-214.
[3]朱晓莺,邓晓杨.苦参凝胶对阴道院内感染常见微生物增殖影响的临床研究[J].中国性科学,2016,25(11):88-90.
[4]朱翠霞,张洪利,康大力.不同炮制方法对黄连抗菌活性的影响[J].中药材,2009,32(6):865-866.
[5]赵军.中药材黄芩对幽门螺杆菌体外抗菌活性的作用分析[J].中国现代药物应用,2017,11(8):195-196.
[6]杨洁,刘萍,武晓玉.苦参提取物对表皮葡萄球菌的体外抗菌活性研究[J].中华医院感染学杂志,2007,17(11):1357-1358.
[7]郝佳,刘哲,张瑜,等.苦参、绿茶及紫苏叶提取物联用对金黄色葡萄球菌的体外活性研究[J].天津中医药大学学报,2014,33(5):296-298.
[8]段智璇,张柏岳,瞿慧琴,等.实时荧光定量PCR法分析苦参对大鼠胃肠道大肠杆菌数量的影响[J].贵阳中医学院学报,2016,38(6):1-8.
[9]郭向华.苦参煎剂对大鼠慢性绿脓杆菌肺炎的影响[D].广西医科大学,2004.
[10]杜思邈,马丽强,孙俊杰,等.苦参提取物体外抗菌实验研究[J].中医药学报,2010,38(3):74-76.
[11]张代玉,徐馨,周庆民,等.苦参生物碱提取物对奶牛乳房炎病原菌的体外抗菌活性研究[J].黑龙江畜牧兽医,2016,59(1):122-123.
[12]房华,汪瑞忠,张晓雪,等.中西药联合对多重耐药铜绿假单胞菌体外抗菌作用研究[J].中国现代医生,2017,55(31):107-110.
[13]陈四玉,杨明川,杨孜生,等. 7种常用抗生素与清开灵配伍前后体外抑菌作用比较[J].时珍国医国药,2017,28(2):280-282.
[14]韩莹.浅析中西药不合理应用引起的毒副作用[J].当代医药论丛,2012,10(12):449-450.