植物单宁调控乳酸菌特性的研究进展及其在生产中的应用
|
摘要
单宁是植物的一种次级代谢产物,能够结合蛋白质形成复合物,在微生物发酵中有特殊生理作用。目前国内外学者对单宁结构、生物学特性和抑菌作用进行了深入研究。本文系统阐述了植物单宁对乳酸菌生长特性的调控作用,包括植物单宁抑菌性、不同种类乳酸菌对植物单宁适应性和代谢途径等,并对单宁在反刍动物营养与饲草加工利用中的应用进行了展望。
Tannins are the secondary metabolites of plants,which can bind proteins together,and have special effects on microbial fermentation. The structure,biological characteristic and bacteriostatic effects of tannins have been deeply researched recently. The regulatory effects of plant tannins on the growth characteristics of lactic acid bacteria were systematically reviewed,including the inhibition of plant tannins and the adaptation and metabolic pathways of different types of lactic acid bacteria to plant tannins. The tannins application in the animal production and forage processing were also discussed.[Chinese Journal of Animal Nutrition,2019,31( 5) : 2063-2068]
Tannins are the secondary metabolites of plants,which can bind proteins together,and have special effects on microbial fermentation. The structure,biological characteristic and bacteriostatic effects of tannins have been deeply researched recently. The regulatory effects of plant tannins on the growth characteristics of lactic acid bacteria were systematically reviewed,including the inhibition of plant tannins and the adaptation and metabolic pathways of different types of lactic acid bacteria to plant tannins. The tannins application in the animal production and forage processing were also discussed.[Chinese Journal of Animal Nutrition,2019,31( 5) : 2063-2068]
引文
[1] MANEC,SOMMERER N,YALCIN T,et al.Assessment of the molecular w eight distribution of tannin fractions through M ALDI-TOF M S analysis of protein-tannin complexes[J]. Analytical Chemistry,2007,79(6):2239-2248.
[2]汪清美,赵丽平.乳酸菌胞外多糖的结构及益生功能研究进展[J].天津农业科学,2015,21(5):19-22.
[3] MCMAHON L R,MCALLISTER T A,BERG B P,et al.A review of the effects of forage condensed tannins on ruminal fermentation and bloat in grazing cattle[J].Canadian Journal of Plant Science,2000,80(3):469-485.
[4]马敏,赵微,李成云.长白山地区5种可利用饲草营养成分及单宁含量动态分析[J].饲料工业,2015,36(21):13-19.
[5] MAKKAR H P S.Effects and fate of tannins in ruminant animals,adaptation to tannins,and strategies to overcome detrimental effects of feeding tannin-rich feeds[J]. Small Ruminant Research,2003,49(3):241-256.
[6] COBLENTZ W K,GRABBER J H. In situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration[J]. Journal of Dairy Science,2013,96(5):3120-3137.
[7] WANG Y,BARBIERI L R,BERG B P,et al.Effects of mixing sainfoin w ith alfalfa on ensiling,ruminal fermentation and total tract digestion of silage[J].Animal Feed Science and Technology,2007,135(3/4):296-314.
[8]王妍君,谢开云,赵祥,等.植物单宁及其对动物的作用研究进展[J].草原与草坪,2011,31(4):82-86,92.
[9] BARRY T N,MCNABB W C. The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants[J]. British Journal of Nutrition,1999,81(4):263-272.
[10]张晓庆,李勇,李发弟,等.红豆草单宁含量对绵羊养分消化率及氮利用的影响[J].畜牧兽医学报,2009,40(3):356-362.
[11] AERTS R J,BARRY T N,MCNABB W C.Polyphenols and agriculture:beneficial effects of proanthocyanidins in forages[J]. Agriculture,Ecosystems&Environment,1999,75(1/2):1-12.
[12] NAUMANN H D,MUIR J P,LAMBERTET B D,et al. Condensed tannins in the ruminant environment:a perspective on biological activity[J]. Journal of Agricultural Sciences,2013,1(1):8-20.
[13] AMMAR H,LOPEZ S,SALEM A Z M,et al. Effect of saliva from sheep that have ingested quebracho tannins on the in vitro rumen fermentation activity to digest tannin-containing shrubs[J]. Animal Feed Science and Technology,2011,163(2/3/4):77-83.
[14] BURGGRAAF V,WAGHORN G,WOODWARD S,et al. Effects of condensed tannins in w hite clover flow ers on their digestion in vitro[J]. Animal Feed Science and Technology,2008,142(1/2):44-58.
[15] WAGHORN G C,SHELTON I D,MCNABB W C.Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep.1.Non-nitrogenous aspects[J]. The Journal of Agricultural Science,1994,123(1):99-107.
[16] WAGHORN G C,MCNABB W C. Consequences of plant phenolic compounds for productivity and health of ruminants[J].Proceedings of the Nutrition Society,2003,62(2):383-392.
[17] KRAUSE D O,SMITH W J M,BROOKER J D,et al.Tolerance mechanisms of streptococci to hydrolysable and condensed tannins[J]. Animal Feed Science and Technology,2005,121(1/2):59-75.
[18]刘秀丽.植物缩合单宁对大肠杆菌的抑制作用及抑菌机理的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2013.
[19]陈敏,何敏.茶多酚抗菌作用研究进展[J].饲料研究,2015(22):10-12.
[20] FERREIRA P R B,MENDES C S O,RODRIGUES C G,et al.Antibacterial activity tannin-rich fraction from leaves of Anacardium humile[J]. Ciência Rural,2012,42(10):1861-1864.
[21] VANBELLE M,TELLER E,FOCANT M. Probiotics in animal nutrition:a review[J].Archiv für Tierernaehrung,1990,40(7):543-567.
[22] MIN B R,ATTWOOD G T,REILLY K,et al. Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep[J]. Canadian Journal of M icrobiology,2002,48(10):911-921.
[23] MIN B R,ATTWOOD G T,MCNABB W C,et al.The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and grow th of rumen bacteria[J]. Animal Feed Science and Technology,2005,121(1/2):45-58.
[24] PILUZZA G,SULAS L,BULLITTA S. Tannins in forage plants and their role in animal husbandry and environmental sustainability:a review[J]. Grass and Forage Science,2014,69(1):32-48.
[25] MOLAN A L,MCNABB W C,HOSKIN S O,et al.Effect of condensed tannins extracted from four forages on the viability of the larvae of deer lungw orms and gastrointestinal nematodes[J].Veterinary Record,2000,147(2):44-48.
[26] TOHNO M,KOBAYASHI H,NOMURA M,et al.Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage[J].Animal Science Journal,2012,83(2):111-120.
[27] HASKARD C A,EL-NEZAMI H S,KANKAANPAAP E,et al.Surface binding of aflatoxin B1by lactic acid bacteria[J]. Applied and Environmental M icrobiology,2001,67(7):3086-3091.
[28]王晓力,张慧杰,孙启忠,等.饲草青贮系统中乳酸菌及其添加剂研究进展[J].中兽医医药杂志,2012,31(6):20-22.
[29] KONDO M,KITA K,YOKOTA H.Effects of tea leaf w aste of green tea,oolong tea,and black tea addition on Sudangrass silage quality and in vitro gas production[J]. Journal of the Science of Food and Agriculture,2004,84(7):721-727.
[30] KAMALAK A,CANBOLATO,爦AHIN M,et al.Effect of oak tannin extract(Artutan)on alfalfa silage composition[C]//Proceedings of the 5th International Balkan Animal Conference.Turkey:[s.n.],2013.
[31]李成云,袁英良,朴光一.缩合单宁对瘤胃挥发性脂肪酸及微生物生长的影响[J].饲料研究,2010(11):5-7.
[32] CAVALLARIN L,ANTONIAZZI S,BORREANI G,et al. Effect of chestnut tannin on protein degradation in lucerne silages[C]//Proceedings of the 19th General meeting of the European Grassland Federation.LaRochelle,France:EGF,2002.
[33] TABACCO E,BORREANI G,CROVETTO G M,et al. Effect of chestnut tannin on fermentation quality,proteolysis,and protein rumen degradability of alfalfa silage[J]. Journal of Dairy Science,2006,89(12):4736-4746.
[34] RODRIGUEZ H,CURIEL J A,LANDETE J M,et al.Food phenolics and lactic acid bacteria[J].International Journal of Food M icrobiology,2009,132(2/3):79-90.
[35] VIVAS N,LONVAUD-FUNEL A,GLORIES Y.Effect of phenolic acids and anthocyanins on grow th,viability and malolactic activity of a lactic acid bacterium[J].Food M icrobiology,1997,14(3):291-299.
[36] VIVAS N,AUGUSTIN M,LONVAUD-FUNEL A.Influence of oak w ood and grape tannins on the lactic acid bacterium Oenococcus oeni(Leuconostoc oenos,8413)[J]. Journal of the Science of Food and Agriculture,2000,80(11):1675-1678.
[37] REGUANT C,BORDONS A,AROLA L,et al.Influence of phenolic compounds on the physiology of Oenococcus oeni from w ine[J]. Journal of Applied M icrobiology,2000,88(6):1065-1071.
[38] ROZES N,AROLA L,BORDONS A. Effect of phenolic compounds on the co-metabolism of citric acid and sugars by Oenococcus oeni from w ine[J]. Letters in Applied M icrobiology,2003,36(5):337-341.
[39] THEOBALD S,PFEIFFER P,ZUBER U,et al. Influence of epigallocatechin gallate and phenolic compounds from green tea on the grow th of Oenococcus oeni[J]. Journal of Applied M icrobiology,2008,104(2):566-572.
[40] TABASCO R,SANCHEZ-PATAN F,MONAGAS M,et al.Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth:resistance and metabolism[J].Food Microbiology,2011,28(7):1345-1352.
[41] ALBERTO M R,FARIAS M E,MANCA DE NADRA M C. Effect of w ine phenolic compounds on Lactobacillus hilgardii 5w viability[J]. Journal of Food Protection,2002,65(1):211-213.
[42] BOSSI A,RINALDUCCI S,ZOLLA L,et al.Effect of tannic acid on Lactobacillus hilgardii analysed by a proteomic approach[J].Journal of Applied M icrobiology,2007,102(3):787-795.
[43] ALBERTO M R,ARENA M E,MANCA DE NADRA M C.Putrescine production from agmatine by Lactobacillus hilgardii:effect of phenolic compounds[J].Food Control,2007,18(8):898-903.
[44] BRASHEARS M M,JARONI D,TRIMBLE J. Isolation,selection,and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157∶H7 in cattle[J].Journal of Food Protection,2003,66(3):355-363.
[45] DOLARA P,LUCERI C,DE FILIPPO C,et al. Red w ine polyphenols influence carcinogenesis,intestinal microflora,oxidative damage and gene expression profiles of colonic mucosa in F344 rats[J]. M utation Research/Fundamental and M olecular M echanisms of M utagenesis,2005,591(1/2):237-246.
[46] CECCONI D,CRISTOFOLETTI M,MILLI A,et al.Effect of tannic acid on Lactobacillus plantarum w ine strain during starvation:a proteomic study[J].Electrophoresis,2009,30(6):957-965.
[47] CURIEL J A,RODRIGUEZ H,DE LAS RIVAS B,et al.Response of a Lactobacillus plantarum human isolate to tannic acid challenge assessed by proteomic analyses[J]. M olecular Nutrition&Food Research,2011,55(10):1454-1465.
[48] REVERON I,RODRIGUEZ H,CAMPOS G,et al.Tannic acid-dependent modulation of selected Lactobacillus plantarum traits linked to gastrointestinal survival[J].PLoS One,2012,8(6):e66473.
[49] REVERON I,JIMENEZ N,CURIEL J A,et al.Differential gene expression by Lactobacillus plantarum WCFS1 in response to phenolic compounds reveals new genes involved in tannin degradation[J]. Applied and Environmental M icrobiology,2017,83(7):e03387-16.
[50] IWAMOTO K,TSURUTA H,NISHITAINI Y,et al.Identification and cloning of a gene encoding tannase(tannin acylhydrolase)from Lactobacillus plantarum ATCC 14917T[J].Systematic and Applied M icrobiology,2008,31(4):269-277.
[51] AGALIAS A,MAGIATIS P,SKALTSOUNIS A L,et al.A new process for the management of olive oil mill w aste w ater and recovery of natural antioxidants[J].Journal of Agricultural and Food Chemistry,2007,55(7):2671-2676.
[52] LAMIA A,MOKTAR H. Fermentative decolorization of olive mill w astew ater by Lactobacillus plantarum[J].Process Biochemistry,2004,39(1):59-65.
[53] JIMENEZ N,ESTEBAN-TORRES M,MANCHENO J M,et al. Tannin degradation by a novel tannase enzyme present in some Lactobacillus plantarum strains[J].Applied and Environmental Microbiology,2014,80(10):2991-2997.
[2]汪清美,赵丽平.乳酸菌胞外多糖的结构及益生功能研究进展[J].天津农业科学,2015,21(5):19-22.
[3] MCMAHON L R,MCALLISTER T A,BERG B P,et al.A review of the effects of forage condensed tannins on ruminal fermentation and bloat in grazing cattle[J].Canadian Journal of Plant Science,2000,80(3):469-485.
[4]马敏,赵微,李成云.长白山地区5种可利用饲草营养成分及单宁含量动态分析[J].饲料工业,2015,36(21):13-19.
[5] MAKKAR H P S.Effects and fate of tannins in ruminant animals,adaptation to tannins,and strategies to overcome detrimental effects of feeding tannin-rich feeds[J]. Small Ruminant Research,2003,49(3):241-256.
[6] COBLENTZ W K,GRABBER J H. In situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration[J]. Journal of Dairy Science,2013,96(5):3120-3137.
[7] WANG Y,BARBIERI L R,BERG B P,et al.Effects of mixing sainfoin w ith alfalfa on ensiling,ruminal fermentation and total tract digestion of silage[J].Animal Feed Science and Technology,2007,135(3/4):296-314.
[8]王妍君,谢开云,赵祥,等.植物单宁及其对动物的作用研究进展[J].草原与草坪,2011,31(4):82-86,92.
[9] BARRY T N,MCNABB W C. The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants[J]. British Journal of Nutrition,1999,81(4):263-272.
[10]张晓庆,李勇,李发弟,等.红豆草单宁含量对绵羊养分消化率及氮利用的影响[J].畜牧兽医学报,2009,40(3):356-362.
[11] AERTS R J,BARRY T N,MCNABB W C.Polyphenols and agriculture:beneficial effects of proanthocyanidins in forages[J]. Agriculture,Ecosystems&Environment,1999,75(1/2):1-12.
[12] NAUMANN H D,MUIR J P,LAMBERTET B D,et al. Condensed tannins in the ruminant environment:a perspective on biological activity[J]. Journal of Agricultural Sciences,2013,1(1):8-20.
[13] AMMAR H,LOPEZ S,SALEM A Z M,et al. Effect of saliva from sheep that have ingested quebracho tannins on the in vitro rumen fermentation activity to digest tannin-containing shrubs[J]. Animal Feed Science and Technology,2011,163(2/3/4):77-83.
[14] BURGGRAAF V,WAGHORN G,WOODWARD S,et al. Effects of condensed tannins in w hite clover flow ers on their digestion in vitro[J]. Animal Feed Science and Technology,2008,142(1/2):44-58.
[15] WAGHORN G C,SHELTON I D,MCNABB W C.Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep.1.Non-nitrogenous aspects[J]. The Journal of Agricultural Science,1994,123(1):99-107.
[16] WAGHORN G C,MCNABB W C. Consequences of plant phenolic compounds for productivity and health of ruminants[J].Proceedings of the Nutrition Society,2003,62(2):383-392.
[17] KRAUSE D O,SMITH W J M,BROOKER J D,et al.Tolerance mechanisms of streptococci to hydrolysable and condensed tannins[J]. Animal Feed Science and Technology,2005,121(1/2):59-75.
[18]刘秀丽.植物缩合单宁对大肠杆菌的抑制作用及抑菌机理的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2013.
[19]陈敏,何敏.茶多酚抗菌作用研究进展[J].饲料研究,2015(22):10-12.
[20] FERREIRA P R B,MENDES C S O,RODRIGUES C G,et al.Antibacterial activity tannin-rich fraction from leaves of Anacardium humile[J]. Ciência Rural,2012,42(10):1861-1864.
[21] VANBELLE M,TELLER E,FOCANT M. Probiotics in animal nutrition:a review[J].Archiv für Tierernaehrung,1990,40(7):543-567.
[22] MIN B R,ATTWOOD G T,REILLY K,et al. Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep[J]. Canadian Journal of M icrobiology,2002,48(10):911-921.
[23] MIN B R,ATTWOOD G T,MCNABB W C,et al.The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and grow th of rumen bacteria[J]. Animal Feed Science and Technology,2005,121(1/2):45-58.
[24] PILUZZA G,SULAS L,BULLITTA S. Tannins in forage plants and their role in animal husbandry and environmental sustainability:a review[J]. Grass and Forage Science,2014,69(1):32-48.
[25] MOLAN A L,MCNABB W C,HOSKIN S O,et al.Effect of condensed tannins extracted from four forages on the viability of the larvae of deer lungw orms and gastrointestinal nematodes[J].Veterinary Record,2000,147(2):44-48.
[26] TOHNO M,KOBAYASHI H,NOMURA M,et al.Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage[J].Animal Science Journal,2012,83(2):111-120.
[27] HASKARD C A,EL-NEZAMI H S,KANKAANPAAP E,et al.Surface binding of aflatoxin B1by lactic acid bacteria[J]. Applied and Environmental M icrobiology,2001,67(7):3086-3091.
[28]王晓力,张慧杰,孙启忠,等.饲草青贮系统中乳酸菌及其添加剂研究进展[J].中兽医医药杂志,2012,31(6):20-22.
[29] KONDO M,KITA K,YOKOTA H.Effects of tea leaf w aste of green tea,oolong tea,and black tea addition on Sudangrass silage quality and in vitro gas production[J]. Journal of the Science of Food and Agriculture,2004,84(7):721-727.
[30] KAMALAK A,CANBOLATO,爦AHIN M,et al.Effect of oak tannin extract(Artutan)on alfalfa silage composition[C]//Proceedings of the 5th International Balkan Animal Conference.Turkey:[s.n.],2013.
[31]李成云,袁英良,朴光一.缩合单宁对瘤胃挥发性脂肪酸及微生物生长的影响[J].饲料研究,2010(11):5-7.
[32] CAVALLARIN L,ANTONIAZZI S,BORREANI G,et al. Effect of chestnut tannin on protein degradation in lucerne silages[C]//Proceedings of the 19th General meeting of the European Grassland Federation.LaRochelle,France:EGF,2002.
[33] TABACCO E,BORREANI G,CROVETTO G M,et al. Effect of chestnut tannin on fermentation quality,proteolysis,and protein rumen degradability of alfalfa silage[J]. Journal of Dairy Science,2006,89(12):4736-4746.
[34] RODRIGUEZ H,CURIEL J A,LANDETE J M,et al.Food phenolics and lactic acid bacteria[J].International Journal of Food M icrobiology,2009,132(2/3):79-90.
[35] VIVAS N,LONVAUD-FUNEL A,GLORIES Y.Effect of phenolic acids and anthocyanins on grow th,viability and malolactic activity of a lactic acid bacterium[J].Food M icrobiology,1997,14(3):291-299.
[36] VIVAS N,AUGUSTIN M,LONVAUD-FUNEL A.Influence of oak w ood and grape tannins on the lactic acid bacterium Oenococcus oeni(Leuconostoc oenos,8413)[J]. Journal of the Science of Food and Agriculture,2000,80(11):1675-1678.
[37] REGUANT C,BORDONS A,AROLA L,et al.Influence of phenolic compounds on the physiology of Oenococcus oeni from w ine[J]. Journal of Applied M icrobiology,2000,88(6):1065-1071.
[38] ROZES N,AROLA L,BORDONS A. Effect of phenolic compounds on the co-metabolism of citric acid and sugars by Oenococcus oeni from w ine[J]. Letters in Applied M icrobiology,2003,36(5):337-341.
[39] THEOBALD S,PFEIFFER P,ZUBER U,et al. Influence of epigallocatechin gallate and phenolic compounds from green tea on the grow th of Oenococcus oeni[J]. Journal of Applied M icrobiology,2008,104(2):566-572.
[40] TABASCO R,SANCHEZ-PATAN F,MONAGAS M,et al.Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth:resistance and metabolism[J].Food Microbiology,2011,28(7):1345-1352.
[41] ALBERTO M R,FARIAS M E,MANCA DE NADRA M C. Effect of w ine phenolic compounds on Lactobacillus hilgardii 5w viability[J]. Journal of Food Protection,2002,65(1):211-213.
[42] BOSSI A,RINALDUCCI S,ZOLLA L,et al.Effect of tannic acid on Lactobacillus hilgardii analysed by a proteomic approach[J].Journal of Applied M icrobiology,2007,102(3):787-795.
[43] ALBERTO M R,ARENA M E,MANCA DE NADRA M C.Putrescine production from agmatine by Lactobacillus hilgardii:effect of phenolic compounds[J].Food Control,2007,18(8):898-903.
[44] BRASHEARS M M,JARONI D,TRIMBLE J. Isolation,selection,and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of Escherichia coli O157∶H7 in cattle[J].Journal of Food Protection,2003,66(3):355-363.
[45] DOLARA P,LUCERI C,DE FILIPPO C,et al. Red w ine polyphenols influence carcinogenesis,intestinal microflora,oxidative damage and gene expression profiles of colonic mucosa in F344 rats[J]. M utation Research/Fundamental and M olecular M echanisms of M utagenesis,2005,591(1/2):237-246.
[46] CECCONI D,CRISTOFOLETTI M,MILLI A,et al.Effect of tannic acid on Lactobacillus plantarum w ine strain during starvation:a proteomic study[J].Electrophoresis,2009,30(6):957-965.
[47] CURIEL J A,RODRIGUEZ H,DE LAS RIVAS B,et al.Response of a Lactobacillus plantarum human isolate to tannic acid challenge assessed by proteomic analyses[J]. M olecular Nutrition&Food Research,2011,55(10):1454-1465.
[48] REVERON I,RODRIGUEZ H,CAMPOS G,et al.Tannic acid-dependent modulation of selected Lactobacillus plantarum traits linked to gastrointestinal survival[J].PLoS One,2012,8(6):e66473.
[49] REVERON I,JIMENEZ N,CURIEL J A,et al.Differential gene expression by Lactobacillus plantarum WCFS1 in response to phenolic compounds reveals new genes involved in tannin degradation[J]. Applied and Environmental M icrobiology,2017,83(7):e03387-16.
[50] IWAMOTO K,TSURUTA H,NISHITAINI Y,et al.Identification and cloning of a gene encoding tannase(tannin acylhydrolase)from Lactobacillus plantarum ATCC 14917T[J].Systematic and Applied M icrobiology,2008,31(4):269-277.
[51] AGALIAS A,MAGIATIS P,SKALTSOUNIS A L,et al.A new process for the management of olive oil mill w aste w ater and recovery of natural antioxidants[J].Journal of Agricultural and Food Chemistry,2007,55(7):2671-2676.
[52] LAMIA A,MOKTAR H. Fermentative decolorization of olive mill w astew ater by Lactobacillus plantarum[J].Process Biochemistry,2004,39(1):59-65.
[53] JIMENEZ N,ESTEBAN-TORRES M,MANCHENO J M,et al. Tannin degradation by a novel tannase enzyme present in some Lactobacillus plantarum strains[J].Applied and Environmental Microbiology,2014,80(10):2991-2997.