95%二甲四氯原药短期重复染毒对大鼠NAG、BUN、Cr的影响
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摘要
目的:采用灌胃法建立95%二甲四氯原药短期重复染毒大鼠模型,通过观察各剂量组大鼠肾脏脏器系数、尿肌酐(Cr)、β-N-乙酰氨基葡萄糖苷酶(NAG);血Cr、尿素氮(BUN);肾NAG的变化,了解95%二甲四氯原药对大鼠肾脏相关指标的影响,为二甲四氯广泛应用于农业生产提供实验依据。方法:SD成年健康大鼠40只,按体重随机分为溶剂对照组、低剂量组、中剂量组、高剂量组,每组10只,雌雄各半。以10m L/kg.bw灌胃,连续28天。染毒结束后,计算大鼠肾脏脏器系数;采用全自动生化分析仪检测血Cr、BUN;采用试剂盒检测尿Cr、NAG、肾NAG。结果:肾脏脏器系数结果显示:雌鼠肾脏脏器系数各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠肾脏脏器系数各剂量组与对照组比较,差异无统计学意义(P>0.05)。尿NAG、Cr结果显示:雌鼠尿NAG各剂量组与对照组比较,差异有统计学意义(F=6.774,P<0.05);雄鼠尿NAG高剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠尿Cr各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠尿Cr各剂量组与对照组比较,差异无统计学意义(P>0.05)。血BUN、Cr结果显示:雌鼠血Cr中剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠血BUN各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠血BUN各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠血Cr各剂量组与对照组比较,差异无统计学意义(P>0.05)。肾NAG结果显示:雄鼠肾NAG中剂量组、高剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠各剂量组肾NAG与对照组比较,差异无统计学意义(P>0.05)。相关性结果显示:尿NAG和肾NAG有相关性(r=0.341,P<0.05);尿Cr和尿NAG有相关性(r=0.374,P<0.05);尿Cr和血Cr有相关性(r=0.379,P<0.05)。结论:95%二甲四氯原药可导致Cr、BUN、NAG的改变,对大鼠肾脏功能造成损伤。通过检测尿Cr值的变化情况,可了解短期重复接触95%二甲四氯原药对肾脏的损伤情况。在农业生产中,应避免长期接触95%二甲四氯原药。
Objective: this experiment was established by intragastric administration of 95% 2-methyl-4-chlorophenoxy acetic acid pesticides of short-term repeated exposed rats model,through the observation of each dose group rats organ coefficient,urinary creatinine( Cr),beta-N-Acetylglucosaminidase( NAG); Cr blood urea nitrogen( BUN); change renal NAG,to understand the effect of 95% 2-methyl-4-chlorophenoxy acetic acid related indexes on rat kidney,2-methyl-4-chlorophenoxy acetic acid is widely used in agricultural production to provide experimental basis. Methods: healthy adult SD 40 rats were randomly divided into,solvent control group,low dose group,middle dose group,high dose group 10 rats in each group,male and female. Half. With 10 m L/kg. bw orally,for 28 days. We observed the meter kidney is the organ coefficient of rats;blood was detected by Cr,BUN by automatic biochemical analyzer; urine Cr test kit,NAG,renal NAG. results: The results showed that the renal index group comparison of female rat kidney coefficient of each dose group and the control,the difference was not statistically significant( P > 0. 05); group kidney organ coefficient of male rats in each dose group with the control,the difference was not statistically significant( P > 0. 05). Urine NAG,Cr results showed that female rats of urinary NAG in each dose group compared with the control group,the difference was statistically significant( F = 6. 774,P < 0. 05); group of male rat urine NAG high dose group and the control,the difference was statistically significant( P < 0. 05); group of female rat urine Cr in each dose group and the control,the difference was not statistically significant( P > 0. 05); group of male rat urine Cr in each dose group and the control,the difference no statistical significance( P > 0. 05). The levels of BUN,Cr showed that female rats blood in Cr group compared with the control group,the difference was statistically significant( P < 0. 05); group of female rats with different doses of BUN blood group and the control,the difference was not statistically significant( P > 0. 05); group of male rats with different doses of BUN blood group and the control,the difference was not statistically significant( P > 0. 05); group of male rats with different doses of Cr blood group and the control,the difference was not statistically significant( P > 0. 05). Renal NAG showed that renal NAG male rats in dose group,high dose and control group. The difference was statistically significant( P < 0. 05);group of female rats in each dose group of renal NAG and the control,the difference was not statistically significant( P > 0. 05).The results showed that the correlation relationship between urinary NAG and renal NAG( r = 0. 341,P < 0. 05); urinary Cr and NA The relationship between G( r = 0. 374,P < 0. 05); there is correlation between urine Cr and blood Cr( r = 0. 379,P <0. 05). Conclusion: 95% 2-methyl-4-chlorophenoxy acetic acid a drug can lead to Cr,BUN,NAG changes,causing damage to the kidney function of rats. The changes of the urinary Cr value,can understand the short-term repeated contact 95% 2-methyl-4-chlorophenoxy acetic acid pesticide in kidney damage. Therefore in agricultural production,should avoid long-term exposure to 95% 2-methyl-4-chlorophenoxy acetic acid a TC.
Objective: this experiment was established by intragastric administration of 95% 2-methyl-4-chlorophenoxy acetic acid pesticides of short-term repeated exposed rats model,through the observation of each dose group rats organ coefficient,urinary creatinine( Cr),beta-N-Acetylglucosaminidase( NAG); Cr blood urea nitrogen( BUN); change renal NAG,to understand the effect of 95% 2-methyl-4-chlorophenoxy acetic acid related indexes on rat kidney,2-methyl-4-chlorophenoxy acetic acid is widely used in agricultural production to provide experimental basis. Methods: healthy adult SD 40 rats were randomly divided into,solvent control group,low dose group,middle dose group,high dose group 10 rats in each group,male and female. Half. With 10 m L/kg. bw orally,for 28 days. We observed the meter kidney is the organ coefficient of rats;blood was detected by Cr,BUN by automatic biochemical analyzer; urine Cr test kit,NAG,renal NAG. results: The results showed that the renal index group comparison of female rat kidney coefficient of each dose group and the control,the difference was not statistically significant( P > 0. 05); group kidney organ coefficient of male rats in each dose group with the control,the difference was not statistically significant( P > 0. 05). Urine NAG,Cr results showed that female rats of urinary NAG in each dose group compared with the control group,the difference was statistically significant( F = 6. 774,P < 0. 05); group of male rat urine NAG high dose group and the control,the difference was statistically significant( P < 0. 05); group of female rat urine Cr in each dose group and the control,the difference was not statistically significant( P > 0. 05); group of male rat urine Cr in each dose group and the control,the difference no statistical significance( P > 0. 05). The levels of BUN,Cr showed that female rats blood in Cr group compared with the control group,the difference was statistically significant( P < 0. 05); group of female rats with different doses of BUN blood group and the control,the difference was not statistically significant( P > 0. 05); group of male rats with different doses of BUN blood group and the control,the difference was not statistically significant( P > 0. 05); group of male rats with different doses of Cr blood group and the control,the difference was not statistically significant( P > 0. 05). Renal NAG showed that renal NAG male rats in dose group,high dose and control group. The difference was statistically significant( P < 0. 05);group of female rats in each dose group of renal NAG and the control,the difference was not statistically significant( P > 0. 05).The results showed that the correlation relationship between urinary NAG and renal NAG( r = 0. 341,P < 0. 05); urinary Cr and NA The relationship between G( r = 0. 374,P < 0. 05); there is correlation between urine Cr and blood Cr( r = 0. 379,P <0. 05). Conclusion: 95% 2-methyl-4-chlorophenoxy acetic acid a drug can lead to Cr,BUN,NAG changes,causing damage to the kidney function of rats. The changes of the urinary Cr value,can understand the short-term repeated contact 95% 2-methyl-4-chlorophenoxy acetic acid pesticide in kidney damage. Therefore in agricultural production,should avoid long-term exposure to 95% 2-methyl-4-chlorophenoxy acetic acid a TC.
引文
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[2]王心如.毒理学基础[M].6版.北京:人民卫生出版社,2012.
[3]Idasiad-Piechocka I,Krzymanski M.The role of tubulointers titial changes in progression of kidney function failure in patients wthchronic glomerulonep-hritis[J].Przegl Lek.1996,53:443-453.
[4]黄振烈,陈润涛,宋向荣,等.大鼠亚急性吸入高效氯氰菊酯的毒性研究[J].中国工业医学杂志,2009,22(5):340-343.
[5]冉海霞,赵海,杜江,等.苗药半截烂对小鼠肝、肾毒性实验的初步研究[J].实验动物科学,2015,32(2):27-30.
[6]李永明.丹参多糖的急性和亚急性毒性试验研究[J].药学研究,2016,35(1):16-18.
[7]成遥,张贺,孟楣,等.新风胶囊肝肾亚急性毒性实验研究[J].安徽医药,2015,19(8):1450-1453.
[8]陈力,熊筱娟,高守红,等.UHPLC-MS/MS法测定大鼠血浆中环磷酰胺及其代谢物的浓度[J].第二军医大学学报,2016,37(9):1063-1069.