短穗兔耳草提取物对高尿酸血症小鼠肾脏保护作用研究
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摘要
目的研究短穗兔耳草提取物对高尿酸血症小鼠肾脏有无保护作用。方法以氧嗪酸钾盐诱导小鼠高尿酸血症模型为实验系统,通过检测血清尿酸和黄嘌呤氧化酶含量和肾脏病理病变等指标,评价短穗兔耳草提取物对高尿酸血症小鼠肾脏的保护作用。结果短穗兔耳草可显著降低模型大鼠血清、尿酸和黄嘌呤氧化酶含量,改善了小鼠肾脏结构的变化。结论短穗兔耳草具有降尿酸活性及肾脏保护作用。
Objective To investigate the effect of Lagotis brachystachys Maxim extract on potassium oxonate-induced hyperuricemia in mice.Methods Three doses of Lagotis brachystachys Maxim extract were administrated to hyperuricemia mice induced by potassium oxonate for 7 days.Serum uric acid levels and hepatic xanthine oxidase( XOD) activities,as well as mRNA expressions of renal urate transpoters( URAT1,GLUT9 and OAT1) were analyzed. Results The contents of serum creatinine,urea nitrogen,uric acid and xanthine oxidase in model rats were significantly decreased,and the change of kidney structure was improved.Conclusion Lagotis brachystachys Maxim extract could obviously reduce the level of serum uric acid in hyperuricemia mice and the effect was related to its regulation of expressions of renal urate transpoters and inhibition of hepatic XOD activities.
Objective To investigate the effect of Lagotis brachystachys Maxim extract on potassium oxonate-induced hyperuricemia in mice.Methods Three doses of Lagotis brachystachys Maxim extract were administrated to hyperuricemia mice induced by potassium oxonate for 7 days.Serum uric acid levels and hepatic xanthine oxidase( XOD) activities,as well as mRNA expressions of renal urate transpoters( URAT1,GLUT9 and OAT1) were analyzed. Results The contents of serum creatinine,urea nitrogen,uric acid and xanthine oxidase in model rats were significantly decreased,and the change of kidney structure was improved.Conclusion Lagotis brachystachys Maxim extract could obviously reduce the level of serum uric acid in hyperuricemia mice and the effect was related to its regulation of expressions of renal urate transpoters and inhibition of hepatic XOD activities.
引文
[1]董晓琴.大黄酸对果糖诱导的高尿酸大鼠的肾脏保护作用研究[J].海峡药学,2016,28(3):42-44.
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[11]ZHU J X,WANG Y,KONG L D,et al.Effects of Biota Orientalis Extract and its Flavonoid Constituents,Quercetin and Rutin on Serum Uric Levels in Oxonate-Induced Mice and Xanthine Dehydrogenase and Xanthine Oxidase Activities in Mouse Liver[J].J Ethnopharmacol,2004,93(1):133-140.
[12]SONG H P,ZHANG H,FU Y,et al.Screening for selective inhibitors of xanthine oxidase from Flos Chrysanthemum using ultrafiltration LC-MS combined with enzyme channel blocking[J].J Chromatogr B Analyt Technol Biomed Life Sci,2014(961):56-61.
[13]HU Q H,ZHU J X,JI J,et al.Fructus Gardenia Extract Ameliorates Oxonate-Induced Hyperuricemia with Renal Dysfunction in Mice by Regulating Organic Ion Transporters and m OIT3[J].Molecules,2013,18(8):8976-8993.
[14]HU Q H,JIAO R Q,WANG X,et al.Simiao pill ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice[J].J Ethnopharmacol,2010,128(3):685-692.
[15]HONG S S,SEO K,LIM S C,et al.Interaction characteristics of flavonoids with human organic anion transporter 1(h OAT1)and 3(h OAT3)[J].Pharmacol Res,2007,56(6):468-473.
[16]朱立然,陈光亮.尿酸转运蛋白研究进展[J].中国临床药理学与治疗学,2012,17(11):1289-1294.
[2]WIJNANDS J M,VIECHTBAUER W,THEVISSEN K,et al.Determinants of the prevalence of gout in the general population:a systematic review and meta-regression[J].Eur J Epidemiol,2015,30(1):19-33.
[3]杨媛,李静,甄健存,等.抗痛风药别嘌呤醇、苯溴马隆及秋水仙碱不良反应报告分析[J].中国医院药学杂志,2013,33(15):1296-1297.
[4]胡庆华,朱继孝,李宁,等.栀子苷对氧嗪酸钾盐致小鼠高尿酸血症的作用及其机制研究[J].中南药学,2013,11(10):721-725.
[5]SCHUMACHER H R JR,BECKER M A,LLOYD E,et al.Febuxostat in the treatment of gout:5-yr findings of the FOCUS efficacy and safety study[J].Rheumatology(Oxford),2009,48(2):188-194.
[6]WANG J,LIU S,MA B,et al.Rapid screening and detection of XOD inhibitors from S.tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC[J].Anal Bioanal Chem,2014,406(28):7379-7387.
[7]SU J,WEI Y,LIU M,et al.Anti-hyperuricemic and nephroprotective effects of Rhizoma Dioscoreae septemlobae extracts and its main component dioscin via regulation of m OAT1,m URAT1 and m OCT2 in hypertensive mice[J].Arch Pharm Res,2014,37(10):1336-1344.
[8]张忠,李科玮,毕阳,等.两种兔耳草提取物的体外抑菌作用[J].食品工业科技,2012,33(9):62-65.
[9]王亚杰,张国文.桑色素对黄嘌呤氧化酶活性的抑制作用[J].食品科学,2014,35(13):143-146.
[10]孙永丽,赵焕新,白虹.HPLC法体外筛选黄嘌呤氧化酶抑制剂[J].药物分析杂志,2014,34(8):1391-1396.
[11]ZHU J X,WANG Y,KONG L D,et al.Effects of Biota Orientalis Extract and its Flavonoid Constituents,Quercetin and Rutin on Serum Uric Levels in Oxonate-Induced Mice and Xanthine Dehydrogenase and Xanthine Oxidase Activities in Mouse Liver[J].J Ethnopharmacol,2004,93(1):133-140.
[12]SONG H P,ZHANG H,FU Y,et al.Screening for selective inhibitors of xanthine oxidase from Flos Chrysanthemum using ultrafiltration LC-MS combined with enzyme channel blocking[J].J Chromatogr B Analyt Technol Biomed Life Sci,2014(961):56-61.
[13]HU Q H,ZHU J X,JI J,et al.Fructus Gardenia Extract Ameliorates Oxonate-Induced Hyperuricemia with Renal Dysfunction in Mice by Regulating Organic Ion Transporters and m OIT3[J].Molecules,2013,18(8):8976-8993.
[14]HU Q H,JIAO R Q,WANG X,et al.Simiao pill ameliorates urate underexcretion and renal dysfunction in hyperuricemic mice[J].J Ethnopharmacol,2010,128(3):685-692.
[15]HONG S S,SEO K,LIM S C,et al.Interaction characteristics of flavonoids with human organic anion transporter 1(h OAT1)and 3(h OAT3)[J].Pharmacol Res,2007,56(6):468-473.
[16]朱立然,陈光亮.尿酸转运蛋白研究进展[J].中国临床药理学与治疗学,2012,17(11):1289-1294.