寒水石不同炮制方法对藏药洁白丸中没食子酸含量的影响
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摘要
目的探讨寒水石不同炮制方法对藏药洁白丸中没食子酸含量的影响。方法采用热制、精制和猛制等方法对藏药洁白丸处方中的寒水石进行炮制,并采用HPLC法测定没食子酸的含量。色谱柱为WondaCract ODS-2C_(18)(250mm×4.6mm,5μm)柱;流速:1.0mL·min~(-1);柱温:40℃;检测波长:270nm;进样量:10μL;以流动相甲醇(A)~(-1)mL·L~(-1)磷酸溶液(B)(7∶93)洗脱15min。结果寒水石不同炮制方法中洁白丸的没食子酸含量为热制6.582mg·g~(-1),RSD值为1.53%;精制6.603mg·g~(-1),RSD值为0.12%;猛制未检出。结论不同炮制方法影响没食子酸含量,其中猛制的寒水石对没食子酸影响显著,不宜与诃子同方使用,为洁白丸合理配方提供理论依据。
Objective To investigate the effects of different processing methods of Hanshuishi on the contents of gallic acid in Jiebai Pills.Methods The Hanshuishi in the prescription of Tibetan medicine Jiebai Pills was processed by means of heating,refining and fierce processing,and HPLC was used to measure the content of gallic acid in different processing products of Jiebai Pills.HPLC conditions were as follows:column WondaCract ODS-2C_(18)(250mm×4.6mm,5μm),elution with mobile phase methanol(A)-1mL·L~(-1)phosphoric acid(7∶93)for 15 min,flow rate 1.0mL·min~(-1),column temperature 40℃,detection wavelength of gallic acid 270nm,injection volume 10μL.Results The contents of gallic acid in Jiebai Pills with different processing methods,heating,refining and fierce processing were significantly different 6.582(RSD=1.53%),6.603mg·g~(-1)(RSD=0.12%)and not detected.Conclusion Different processed methods have an effect on gallic acid content,in which fierce processed Hanshuishi has an obvious influence on gallic acid,it is not suitable to use with Terminalia chebula Retz.This study provides a theoretical basis for the rational formulation of Jiebai Pills.
Objective To investigate the effects of different processing methods of Hanshuishi on the contents of gallic acid in Jiebai Pills.Methods The Hanshuishi in the prescription of Tibetan medicine Jiebai Pills was processed by means of heating,refining and fierce processing,and HPLC was used to measure the content of gallic acid in different processing products of Jiebai Pills.HPLC conditions were as follows:column WondaCract ODS-2C_(18)(250mm×4.6mm,5μm),elution with mobile phase methanol(A)-1mL·L~(-1)phosphoric acid(7∶93)for 15 min,flow rate 1.0mL·min~(-1),column temperature 40℃,detection wavelength of gallic acid 270nm,injection volume 10μL.Results The contents of gallic acid in Jiebai Pills with different processing methods,heating,refining and fierce processing were significantly different 6.582(RSD=1.53%),6.603mg·g~(-1)(RSD=0.12%)and not detected.Conclusion Different processed methods have an effect on gallic acid content,in which fierce processed Hanshuishi has an obvious influence on gallic acid,it is not suitable to use with Terminalia chebula Retz.This study provides a theoretical basis for the rational formulation of Jiebai Pills.
引文
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[6]李建梅,买买提·霍吉,希尔艾力·吐尔逊.HPLC法同时测定西诃蜜膏中没食子酸和鞣花酸的含量[J].中国药房,2016,27(18):2546-2548.
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[11]黄一平,段金廒,季锡中.藏药牦牛骨炮制研究[J].中国中药杂志,2006,31(20):1735-1736.
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[15]朱林燕,孔子铭,谢建峰,等.藏药二十一味寒水石丸中的总鞣质、没食子酸和鞣花酸的含量测定[J].西南民族大学学报:自然科学版,2014,40(3):388-393.
[16]郑玉忠,张振霞,董婷霞,等.余甘子药材HPLC指纹图谱及其没食子酸和鞣花酸含量分析[J].中国实验方剂学杂志,2013,19(23):94-99.
[17]张海伟,张艺,杨继家,等.HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量[J].中国实验方剂学杂志,2013,19(10):95-97.
[18]刘艳辉,吴昆仑,秦智莹,等.HPLC法测定泽漆含漱液中芦丁和没食子酸的含量[J].西北药学杂志,2017,32(1):45-48.
[19]Ohno Y,Fukuda K,Takemura G,et al.Induction of apoptosis by gallic acid in lung cancer cells[J].Anticancer Drugs,1999,10(9):845-851.
[20]国家中医药管理局《中华本草》编委会.中华本草:藏药卷[M].上海:上海科学技术出版社,2002:33.
[21]姜建锋,杜玉枝,魏立新,等.藏药南寒水石热制炮制工艺研究[J].中国中药杂志,2011,36(6):693-686.
[22]夏振江,魏立新,杜玉枝,等.南寒水石质量标准研究[J].中南药学,2010,8(9):654-657.
[23]夏振江,魏立新,杜玉枝,等.北寒水石质量标准研究[J].时珍国医国药,2010,21(7):1658-1659.
[2]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:1290-1291.
[3]王烨,宋珍,陈玉萍.智托洁白丸治疗消化性溃疡的临床观察[J].中国伤残医学,2010,18(2):77-78.
[4]陈玉平,王淑云,陆海燕,等.智托洁白丸治疗消化性溃疡的临床观察[J].齐齐哈尔医学院学报,2008,30(5):555-556.
[5]柯发敏,张开莲.没食子酸的研究进展[J].泸州医学院学报,2011,34(4):440-442.
[6]李建梅,买买提·霍吉,希尔艾力·吐尔逊.HPLC法同时测定西诃蜜膏中没食子酸和鞣花酸的含量[J].中国药房,2016,27(18):2546-2548.
[7]陈朝军,陆景坤,高甜,等.南寒水石炮制工艺及药效学初探[J].中国实验方剂学杂志,2013,19(1):191-194.
[8]全正香,杜玉枝,魏立新,等.炮制对藏药南寒水石钙溶出率的影响[J].中国实验方剂学杂志,2011,17(11):16-18.
[9]青海省食品药品监督管理局.青海省藏药炮制规范[S].西宁:青海省民族出版社,2012:23.
[10]李琼,汪付田,张琳.诃子去核对药典品种洁白丸疗效的影响[J].甘肃中医,2006,19(9):45-46.
[11]黄一平,段金廒,季锡中.藏药牦牛骨炮制研究[J].中国中药杂志,2006,31(20):1735-1736.
[12]洛桑多吉.藏药材炮制规范[M].拉萨:西藏人民出版社,2008:65.
[13]帝玛尔·丹增彭措.晶珠本草[M].上海:上海科学技术出版社,2012:77-79.
[14]陈小雪,黄晓汕,黄耀广.HPLC法测定玛木然止泻胶囊中盐酸小檗碱与没食子酸的含量[J].西北药学杂志,2017,32(1):42-45.
[15]朱林燕,孔子铭,谢建峰,等.藏药二十一味寒水石丸中的总鞣质、没食子酸和鞣花酸的含量测定[J].西南民族大学学报:自然科学版,2014,40(3):388-393.
[16]郑玉忠,张振霞,董婷霞,等.余甘子药材HPLC指纹图谱及其没食子酸和鞣花酸含量分析[J].中国实验方剂学杂志,2013,19(23):94-99.
[17]张海伟,张艺,杨继家,等.HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量[J].中国实验方剂学杂志,2013,19(10):95-97.
[18]刘艳辉,吴昆仑,秦智莹,等.HPLC法测定泽漆含漱液中芦丁和没食子酸的含量[J].西北药学杂志,2017,32(1):45-48.
[19]Ohno Y,Fukuda K,Takemura G,et al.Induction of apoptosis by gallic acid in lung cancer cells[J].Anticancer Drugs,1999,10(9):845-851.
[20]国家中医药管理局《中华本草》编委会.中华本草:藏药卷[M].上海:上海科学技术出版社,2002:33.
[21]姜建锋,杜玉枝,魏立新,等.藏药南寒水石热制炮制工艺研究[J].中国中药杂志,2011,36(6):693-686.
[22]夏振江,魏立新,杜玉枝,等.南寒水石质量标准研究[J].中南药学,2010,8(9):654-657.
[23]夏振江,魏立新,杜玉枝,等.北寒水石质量标准研究[J].时珍国医国药,2010,21(7):1658-1659.