丛枝菌根真菌应用技术研究进展
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摘要
丛枝菌根(AM)共生体系能够改善植物营养状况,增强植物对各种逆境胁迫的耐受性,其在农业和生态环境方面的应用得到广泛关注.近年来,在AM真菌(AMF)应用技术和田间试验方面取得了许多重要成果.本文在介绍AMF种质资源库、商业化菌剂生产及相关专利申报情况的基础上,结合实例从菌剂生产、接种技术、接种效应影响因素等方面综述了AMF应用技术的理论与实践,包括国内外近年来菌根技术在农业、园艺、生态修复等方面的应用,最后提出尚待系统深入研究的AMF应用领域中的关键科学和技术问题,旨在为菌根技术的发展和推广应用提供参考.
Arbuscular mycorrhizal(AM) symbiosis facilitates plant mineral nutrient acquisition and plays key roles in plant adaptation to environmental stresses. The application of AM fungi is a component of sustainable agriculture and ecological restoration. We introduced the current status of AM fungi collections, production of commercial inocula and AM fungi related patents, summarized the research advances in inoculum production, inoculation techniques, and factors influencing the success of inoculation practice in the field, based on case studies of mycorrhizal technology in agriculture, horticulture, and ecological restoration. Finally, we proposed some basic scientific questions and technical bottleneck that deserve futher studies, to promote the development and application of mycorrhizal technologies.
Arbuscular mycorrhizal(AM) symbiosis facilitates plant mineral nutrient acquisition and plays key roles in plant adaptation to environmental stresses. The application of AM fungi is a component of sustainable agriculture and ecological restoration. We introduced the current status of AM fungi collections, production of commercial inocula and AM fungi related patents, summarized the research advances in inoculum production, inoculation techniques, and factors influencing the success of inoculation practice in the field, based on case studies of mycorrhizal technology in agriculture, horticulture, and ecological restoration. Finally, we proposed some basic scientific questions and technical bottleneck that deserve futher studies, to promote the development and application of mycorrhizal technologies.
引文
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[3] Kuhn G, Hijri M, Sanders IR. Evidence for the evolution of multiple genomes in arbuscular mycorrhizal fungi. Nature, 2001, 414: 745-748
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[6] Tisserant E, Malbreil M, Kuo A, et al. Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis. Proceedings of the National Aca-demy of Sciences of the United States of America, 2013, 110: 20117-20122
[7] Kiers ET, Duhamel M, Beesetty Y, et al. Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis. Science, 2011, 333: 880-882
[8] Salvioli A, Bonfante P. Systems biology and “omics” tools: A cooperation for next-generation mycorrhizal studies. Plant Science, 2013, 203: 107-114
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[34] Ziane H, Meddad-Hamza A, Beddiar A, et al. Effects of arbuscular mycorrhizal fungi and fertilization levels on industrial tomato growth and production. International Journal of Agriculture and Biology, 2017, 19: 341-347
[35] Wu Y-S (吴亚胜), Guo S-R (郭世荣), Sun J (孙锦), et al. Effects of exogenous spermidine and arbuscular mycorrhizal fungi on plant growth of cucumber. Chinese Journal of Applied Ecology (应用生态学报), 2018, 29(3): 891-898 (in Chinese)
[36] Tarraf W, Ruta C, Tagarelli A, et al. Influence of arbuscular mycorrhizae on plant growth, essential oil production and phosphorus uptake of Salvia officinalis L. Industrial Crops and Products, 2017, 107: 144-153
[37] Xie W, Hao Z, Zhou X, et al. Arbuscular mycorrhiza facilitates the accumulation of glycyrrhizin and liquiritin in Glycyrrhiza uralensis under drought stress. Mycorrhiza, 2018, 28: 285-300
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[2] Bender SF, Wagg C, van der Heijden MGA. An underground revolution: Biodiversity and soil ecological engineering for agricultural sustainability. Trends in Ecology and Evolution, 2016, 31: 440-452
[3] Kuhn G, Hijri M, Sanders IR. Evidence for the evolution of multiple genomes in arbuscular mycorrhizal fungi. Nature, 2001, 414: 745-748
[4] Singh RK, Dai O, Nimasow G. Effect of arbuscular mycorrhizal (AM) inoculation on growth of Chili plant in organic manure amended soil. African Journal of Microbiology Research, 2011, 5: 5004-5012
[5] Asmelash F, Bekele T, Birhane E. The potential role of arbuscular mycorrhizal fungi in the restoration of degraded lands. Frontiers in Microbiology, 2016, 7: 1095, doi: 10.3389/fmicb.2016.01095
[6] Tisserant E, Malbreil M, Kuo A, et al. Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis. Proceedings of the National Aca-demy of Sciences of the United States of America, 2013, 110: 20117-20122
[7] Kiers ET, Duhamel M, Beesetty Y, et al. Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis. Science, 2011, 333: 880-882
[8] Salvioli A, Bonfante P. Systems biology and “omics” tools: A cooperation for next-generation mycorrhizal studies. Plant Science, 2013, 203: 107-114
[9] Gianinazzi S, Gollotte A, Binet MN, et al. Agroecology: The key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza, 2010, 20: 519-530
[10] Rillig MC, Aguilar-Trigueros CA, Bergmann J, et al. Plant root and mycorrhizal fungal traits for understanding soil aggregation. New Phytologist, 2015, 205:1385-1388
[11] Wang Y-S (王幼珊), Zhang S-B (张淑彬), Zhang M-Q (张美庆). Arbuscular mycorrhizal fungi and germplasm resources in China. Beijing: China Agriculture Press, 2012 (in Chinese)
[12] Gerdemann JW, Nicolson TH. Spores of mycorrhizal endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society, 1963, 46: 235-244
[13] Daniels BA, Skipper HD. Methods for the recovery and quantitative estimation of propagules from soi// Korma-nik PP, eds. Methods and Principles of Mycorrhizal Research. Saint Paul, MI, USA: American Society for Phytopathology, 1982: 29-37
[14] Gilmore AE. Phytomycetous mycorrhizal organisms collected by open-pot culture methods. Hilgardia, 1968, 39: 87-105
[15] Crush JR, Hay MJ. A technique for growing mycorrhizal clover in solution culture. New Zealand Journal of Agricultural Research, 1981, 24: 371-372
[16] Mosse B. The establishment of vesicular-arbuscular mycorrhiza under asetic conditions. Journal of General Microbiology, 1962, 27: 509-520
[17] Wang Y-S (王幼珊), Liu R-J (刘润进). A checklist of arbuscular mycorrhizal fungi in the recent taxonomic system of Glomeromycota. Mycosystema (菌物学报),2017, 36(7): 820-850 (in Chinese)
[18] Dalpe Y, Monreal M. Arbuscular mycorrhiza inoculum to support sustainable cropping systems. Crop Management, 2004, 3: 1-11
[19] Masula E, Vassilev N. A contribution to set a legal framework for biofertilisers. Appled Microbiology and Biotechnology, 2014, 98: 6599-6607
[20] Berruti A, Lumini E, Balestrini R, et al. Arbuscular mycorrhizal fungi as natural biofertilizers: Let’s benefit from past successes. Frontiers in Microbiology, 2015, 6: 1559, doi: 10.3389/fmicb.2015.01559
[21] Herrmann L, Lesueur D. Challenges of formulation and quality of biofertilizers for successful inoculation. Applied Microbiology and Biotechnology, 2013, 97: 8859-8873
[22] IJdo M, Cranenbrouck S, Declerck S. Methods for large-scale production of AM fungi: Past, present, and future. Mycorrhiza, 2011, 21: 1-16
[23] Vosátka M, Látr A, Gianinazzi S, et al. Development of arbuscular mycorrhizal biotechnology and industry: Current achievements and bottlenecks. Symbiosis, 2012, 58: 29-37
[24] Oliveira RS, Rocha I, Ma Y, et al. Seed coating with arbuscular mycorrhizal fungi as an ecotechnological approach for sustainable agricultural production of common wheat (Triticum aestivum L.). Journal of Toxicology and Environmental Health Part A, 2016, 79: 329-337
[25] Rasoamampionona B, Rabeharisoa L, Andrianjaka A, et al. Arbuscular mycorrhizae in Malagasy cropping systems. Biological Agriculture & Horticulture, 2008, 25: 327-37
[26] Gomez-Roldan V, Fermas S, Brewer PB, et al. Strigolactone inhibition of shoot branching. Nature, 2008, 455: 189-194
[27] Kapulnik Y, Delaux PM, Resnick N, et al. Strigolactones affect lateral root formation and root-hair elongation in Arabidopsis. Planta, 2011, 233: 209-216
[28] Ceballos I, Ruiz M, Fernández C, et al. The in vitro mass-produced model mycorrhizal fungus, Rhizophagus irregularis, significantly increases yields of the globally important food security crop cassava. PLoS One, 2013, 8(8): e70633
[29] Krak K, Janou?kov昣 M, Caklov昣 P, et al. Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNA. Applied and Environmental Microbiology, 2012, 78: 3630-3637
[30] Selvakumar G, Shagol CC, Kim K, et al. Spores associa-ted bacteria regulates maize root K+/Na+ ion homeostasis to promote salinity tolerance during arbuscular mycorrhizal symbiosis. BMC Plant Biology, 2018, 18: 109, doi: 10.1186/s12870-018-1317-2
[31] Tian H, Drijber R, Zhang J, et al. Impact of long-term nitrogen fertilization and rotation with soybean on the diversity and phosphorus metabolism of indigenous arbuscular mycorrhizal fungi within the roots of maize (Zea mays L.). Agriculture, Ecosystems and Environment, 2013, 164: 53-61
[32] Antunes PM, Koch AM, Dunfield KE, et al. Influence of commercial inoculation with Glomus intraradices on the structure and functioning of an AM fungal community from an agricultural site. Plant and Soil, 2009, 317: 257-266
[33] Zhang X, Chen B, Ohtomo R. Mycorrhizal effects on growth, P uptake and Cd tolerance of the host plant vary among different AM fungal species. Soil Science and Plant Nutrition, 2015, 61: 359-368
[34] Ziane H, Meddad-Hamza A, Beddiar A, et al. Effects of arbuscular mycorrhizal fungi and fertilization levels on industrial tomato growth and production. International Journal of Agriculture and Biology, 2017, 19: 341-347
[35] Wu Y-S (吴亚胜), Guo S-R (郭世荣), Sun J (孙锦), et al. Effects of exogenous spermidine and arbuscular mycorrhizal fungi on plant growth of cucumber. Chinese Journal of Applied Ecology (应用生态学报), 2018, 29(3): 891-898 (in Chinese)
[36] Tarraf W, Ruta C, Tagarelli A, et al. Influence of arbuscular mycorrhizae on plant growth, essential oil production and phosphorus uptake of Salvia officinalis L. Industrial Crops and Products, 2017, 107: 144-153
[37] Xie W, Hao Z, Zhou X, et al. Arbuscular mycorrhiza facilitates the accumulation of glycyrrhizin and liquiritin in Glycyrrhiza uralensis under drought stress. Mycorrhiza, 2018, 28: 285-300
[38] Ahmad H, Hayat S, Ali M, et al. The combination of arbuscular mycorrhizal fungi inoculation (Glomus versiforme) and 28-homobrassinolide spraying intervals improves growth by enhancing photosynthesis, nutrient absorption, and antioxidant system in cucumber (Cucumis sativus L.) under salinity. Ecology and Evolution, 2018, 8: 5724-5740
[39] Domokos E, Jakab-Farkas L, Darko B, et al. Increase in Artemisia annua plant biomass artemisinin content and guaiacol peroxidase activity using the arbuscular mycorrhizal fungus Rhizophagus irregularis. Frontiers in Plant Science, 2018, 9: 478, doi: 10.3389/fpls.2018.00478
[40] Lehmann A,Veresoglou SD, Rillig MC, et al. Arbuscular mycorrhizal influence on zinc nutrition in crop plants: A meta-analysis. Soil Biology and Biochemistry, 2014, 69: 123-131
[41] Lehmann A, Rillig MC. Arbuscular mycorrhizal contribution to copper, manganese and iron nutrient concentrations in crops: A meta-analysis. Soil Biology and Biochemistry, 2015, 81: 147-158
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