电针对慢性疲劳综合征大鼠行为学及大鼠脑组织病理形态学的影响
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摘要
目的观察头部电针对复合因素刺激所致慢性疲劳综合征大鼠的行为学改变和海马、下丘脑组织形态学改变的影响。方法选取SD大鼠36只,采用随机数字表法分为正常组、模型组和针刺组,每组各12只。模型组和针刺组采用复合因素刺激的方法建立慢性疲劳综合征模型,针刺组电针百会、宁神、双侧感觉区,观察大鼠在旷场中的活动情况和大鼠海马、下丘脑组织形态变化情况。结果治疗前,与正常组比较,模型组和针刺组大鼠的水平活动和垂直活动明显减少(P<0.01);治疗后,与模型组比较,针刺组大鼠的水平活动和垂直活动明显增多(P<0.01)。HE染色显示,治疗前,与正常组比较,模型组和针刺组大鼠海马、下丘脑神经细胞呈现形态上和数量上的病理改变,凋亡细胞数量增多,毛细血管水肿;治疗后,与模型组比较,针刺组大鼠海马、下丘脑神经细胞的病理改变有所改善。结论头部电针可以明显改善慢性疲劳综合征模型大鼠的中枢性疲劳状态,该作用机制可能与电针对海马、下丘脑神经元的修复有关。
Objective To observe the effect of head electroacupuncture on behavioral changes and morphological changes of hippocampus and hypothalamus in rats with chronic fatigue syndrome induced by composite factors stimulation. Methods Thirty-six SD rats were selected and divided into the normal group, the model group and the acupuncture group by random number table, with 12 rats in each group. The model group and the acupuncture group used the composite factors stimulation to establish the chronic fatigue syndrome model. The acupuncture group was treated with electroacupuncture of baihui, ningshen and bilateral sensory areas to observe the activity of rats in the open field and the changes of tissue morphology in hippocampus and hypothalamus of the rats. Results Before treatment, the horizontal activity and vertical activity of the rats in the model group and the acupuncture group were significantly reduced compared with the normal group(P < 0.01). After treatment, the horizontal activity and vertical activity of the rats in the acupuncture group were significantly increased(P < 0.01). HE staining showed that the hippocampus and hypothalamus neurons in the model group and the acupuncture group showed morphological and quantitative pathological changes before treatment. The number of apoptotic cells and capillary edema was increased in the model group and the acupuncture group. The pathological changes in the hippocampus and the hypothalamus of the rats in the acupuncture group were improved after treatment. Conclusion Head electroacupuncture can obviously improve the central fatigue state of model rats with chronic fatigue syndrome, which may be related to the repair of neurons in the hippocampus and hypothalamus by electroacupuncture.
Objective To observe the effect of head electroacupuncture on behavioral changes and morphological changes of hippocampus and hypothalamus in rats with chronic fatigue syndrome induced by composite factors stimulation. Methods Thirty-six SD rats were selected and divided into the normal group, the model group and the acupuncture group by random number table, with 12 rats in each group. The model group and the acupuncture group used the composite factors stimulation to establish the chronic fatigue syndrome model. The acupuncture group was treated with electroacupuncture of baihui, ningshen and bilateral sensory areas to observe the activity of rats in the open field and the changes of tissue morphology in hippocampus and hypothalamus of the rats. Results Before treatment, the horizontal activity and vertical activity of the rats in the model group and the acupuncture group were significantly reduced compared with the normal group(P < 0.01). After treatment, the horizontal activity and vertical activity of the rats in the acupuncture group were significantly increased(P < 0.01). HE staining showed that the hippocampus and hypothalamus neurons in the model group and the acupuncture group showed morphological and quantitative pathological changes before treatment. The number of apoptotic cells and capillary edema was increased in the model group and the acupuncture group. The pathological changes in the hippocampus and the hypothalamus of the rats in the acupuncture group were improved after treatment. Conclusion Head electroacupuncture can obviously improve the central fatigue state of model rats with chronic fatigue syndrome, which may be related to the repair of neurons in the hippocampus and hypothalamus by electroacupuncture.
引文
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[2]Brenu EW,van Driel ML,Staines DR,et al.Immunological abnormalities as potential biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis[J].J Transl Med,2011,28(9):81.
[3]Meeus M,Nijs J,Van Mol E,et al.Role of psychological aspects in both chronic pain and in daily functioning in chronic fatigue syndrome:a prospective longitudinal study[J].Clin Rheumatol,2012,31(6):921-929.
[4]Shan ZY,Kwiatek R,Burnet R,et al.Progressive brain changes in patients with chronic fatigue syndrome:a longitudinal MRI study[J].J Magn Reson Imaging,2016,44(5):1301-1311.
[5]张维,彭唯娜,刘志顺.针刺治疗慢性疲劳综合征的系统评价[J].循证医学,2009,9(1):41-47.
[6]呼德尔朝鲁,霍得逸,卢莎.单纯游泳运动构建慢性疲劳综合征小鼠模型[J].世界最新医学信息文摘,2017,17(A0):85,89.
[7]张丽,张振贤,黄瑶,等.不同慢性应激综合模式下慢性疲劳动物实验研究概况[J].中国中西医结合杂志,2016,36(8):1015-1020.
[8]古春青,王著敏,赵铎.红景天对复合应激因素致慢性疲劳大鼠体重及一般行为学的影响[J].中国处方药,2016,14(4):29-30.
[9]高怀林,吴以岭,朱慧明,等.慢性疲劳综合征动物模型的建立及对血管内皮功能的影响[J].中国中医基础医学杂志,2009,15(12):904-907.
[10]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003.
[11]时烨.电针头穴刺激对健康人大脑各功能区变化的f MRI研究[D].南京:南京中医药大学,2016.
[12]姜思竹.基于静息态f MRI和DTI探讨头针治疗脑卒中偏瘫的机制研究[D].北京:北京中医药大学,2017.
[13]孙忠人,王玉琳,张瑞,等.针灸医案精选[M].北京:中国中医药出版社,2012.
[14]蔡思琪,李沅衡,黎嘉,等.鼠类行为学实验方法研究进展[J].医学综述,2018,24(5):916-920.
[15]周全红,康明祥.慢性疲劳综合征神经内分泌机制的研究进展[J].西南军医,2010,12(3):516-518.
[16]王杨.慢性疲劳综合征患者HPA轴及5-HT系统的神经内分泌机制及中西医治疗进展[J].中国中医药现代远程教育,2016,14(19):143-146.
[17]Morris G,Anderson G,Maes M.Hypothalamic-pituitaryadrenal hypofunction in myalgic encephalomyelitis(ME)/chronic fatigue syndrome(CFS)as a Consequence of Activated Immune-Inflammatory and Oxidative and Nitrosative Pathways[J].Mol Neurobiol,2017,54(9):6806-6819.
[18]袁权.电针调整慢性疲劳大鼠HPA轴功能的下丘脑CRH机制[D].成都:成都中医药大学,2012.