毛菊苣不同生长期不同药用部位秦皮乙素含量测定
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摘要
探究毛菊苣不同生长期不同药用部位活性成分秦皮乙素的含量,为确定其最合适的采收季节提供理论依据.本研究于5、6、7、8月采集毛菊苣根、茎、叶等样品,采用高效液相色谱法测定秦皮乙素含量.色谱条件:色谱柱为waters Xbridge C_(18)(4.6 mm×250 mm,4μm);柱温为25℃;流动相为甲醇-0.2%甲酸(15∶85);流速为1 mL/min,检测波长为345 nm.研究结果表明,毛菊苣根、茎、叶等不同部位秦皮乙素含量存在极显著性差异(p=0.000),其中茎中含量均较高,其次是根和叶;不同生长期毛菊苣根、茎和叶中秦皮乙素的含量也存在显著性差异(p=0.047),其中6月达到最高值(分别为16.188、24.149和10.455μg·g~(-1)),含量符合以下规律:5月<8月<7月<6月.毛菊苣不同药用部位不同生长期秦皮乙素含量随着生长发育进程而发生变化.考虑毛菊苣全草入药时以秦皮乙素含量为指标的话,以6～7月枝叶茂盛时采收,有效成分含量最高.
To study contents of aesculetin in different medicinal parts and growth stage of Cichorum glandulosum Boiss.et Huet and to provide a theoretical basis for confirming an optimal recovery season,we collected the root,stem and leaf samples from May to August for this study,High performance liquid chromatography(HPLC) method was used to determined aesculetin contents.The waters Xbridge C18(4.6 mm×250 mm,4 μm) was used; the column temperature was set at 25 ℃;the detection wavelength was 345 nm;the mobile phase consisted of methanol-0.2% formic acid,and the flow rate was 1 mL/min.The results showed that there were significant differences in the content of aesculetin in roots,stems and leaves of Cichorum glandulosum Boiss.et Huet(p=0.000),in which the aesculetin was the highest in stems,then the roots and leaves.The content of aesculetin in roots,stems and leaves of Cichorum glandulosum Boiss.et Huetat at different growth stages was also significantly different and reached the highest level in June(16.188,24.149 and 10.455 μg·g~(-1) respectively).The content conforms to the following rules:May
To study contents of aesculetin in different medicinal parts and growth stage of Cichorum glandulosum Boiss.et Huet and to provide a theoretical basis for confirming an optimal recovery season,we collected the root,stem and leaf samples from May to August for this study,High performance liquid chromatography(HPLC) method was used to determined aesculetin contents.The waters Xbridge C18(4.6 mm×250 mm,4 μm) was used; the column temperature was set at 25 ℃;the detection wavelength was 345 nm;the mobile phase consisted of methanol-0.2% formic acid,and the flow rate was 1 mL/min.The results showed that there were significant differences in the content of aesculetin in roots,stems and leaves of Cichorum glandulosum Boiss.et Huet(p=0.000),in which the aesculetin was the highest in stems,then the roots and leaves.The content of aesculetin in roots,stems and leaves of Cichorum glandulosum Boiss.et Huetat at different growth stages was also significantly different and reached the highest level in June(16.188,24.149 and 10.455 μg·g~(-1) respectively).The content conforms to the following rules:May
引文
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[7] 胡君萍,迪丽拜尔·马木提,李渊,等.UPLC同时测定维药毛菊苣和菊苣中5种化学成分的含量[J].中国实验方剂学杂志,2014(17):65-68.
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[9] 杨巧丽,刘燕,史玉柱,等.毛菊苣水提取物急性毒性及其保肝作用研究[J].中国现代应用药学,2017 (7):957-963.
[10] 陈海君,秦惠玉,龙飞,等.超滤亲和结合液相色谱-质谱联用和分子对接技术筛选毛菊苣种子中高亲和性α-葡萄糖苷酶抑制剂[J].分析化学,2017(6):889-897.
[11] 胡利萍,张钰,秦冬梅,等.维药毛菊苣95%乙醇提取物对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用及机制研究[J].中国药房,2017,28 (34):4830-4834.
[12] GILANI A H,JABAZ K H,SHARH B H.Esculetin Prevents liver damage induced by paracetamol and CCl4[J].Pharm Res,1998,37(1):31.
[13] LIN W L,WANG C J,TSAL Y Y,et al.Inhibitory effect of esculetin on oxidative damage induced by t-butyl hydroperoxide in rat liver[J].Arch Toxicol,2000,74(8):467.
[14] 陈晓蕾,汤立建,李庆林,等.秦皮醇提取物体外抗乳腺癌细胞增殖的研究[J].中国药房,2007,18(15):1124.
[15] WANG C J,HSIEH Y J,CHU C Y,et al.Inhibition of cell cycle progression in human leukemia HL-60 cells by esculin[J].Cancer Letters,2002,183:163.
[16] KUO H C,LEE H J,HU C C,et al.Enhancement of esculetin on Taxol-induced apoptosis in human hepatoma HepG2 cells[J].Toxicology and Applied Pharmacology,2006,210(1/2):55-62.
[17] 伟忠民,王晶.秦皮乙素对人肝癌HepG2细胞体外增殖的影响及机制探讨[J].山东医药,2012,52(39):24-26.
[18] 梁敏.秦皮抗氧化成分的分离及其活性研究[J].食品工业科技,2006,27(3):64.
[19] 吴涛,仲婕,信学雷,等.毛菊苣种子质量标准研究[J].时珍国医国药,2010,21(3):705-707.
[2] AHMED B,ALHOWIRINY T A,SIDDIQUI A B.Antihepatotoxic activity of seeds of Cichorium intybus[J].Journal of Ethnopharmacology,2003,87(2):237-240.
[3] 吴汉夔,樊玉平,巴杭,等.毛菊苣种子精油化学成分的气相色谱-质谱法分析[J].光谱实验室,2005,22(4):695.
[4] 国家中医药管理局《中华本草》编委会.中华本草(维吾尔药卷)[M].上海:上海科学技术出版社,2005:326.
[5] 中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品质量标准(维吾尔药分册)[S].乌鲁木齐:新疆科技卫生出版社,1999.
[6] 杨建华,李渊,闫冬,等.维药毛菊苣对四氯化碳致小鼠肝损伤的保护作用[J].时珍国医国药,2012,23 (10):2383-2386.
[7] 胡君萍,迪丽拜尔·马木提,李渊,等.UPLC同时测定维药毛菊苣和菊苣中5种化学成分的含量[J].中国实验方剂学杂志,2014(17):65-68.
[8] 杨巧丽,王雪,马雪萍,等.维药毛菊苣水提取物抗炎作用的实验研究[J].中国医药导报,2016 (24):27-30.
[9] 杨巧丽,刘燕,史玉柱,等.毛菊苣水提取物急性毒性及其保肝作用研究[J].中国现代应用药学,2017 (7):957-963.
[10] 陈海君,秦惠玉,龙飞,等.超滤亲和结合液相色谱-质谱联用和分子对接技术筛选毛菊苣种子中高亲和性α-葡萄糖苷酶抑制剂[J].分析化学,2017(6):889-897.
[11] 胡利萍,张钰,秦冬梅,等.维药毛菊苣95%乙醇提取物对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用及机制研究[J].中国药房,2017,28 (34):4830-4834.
[12] GILANI A H,JABAZ K H,SHARH B H.Esculetin Prevents liver damage induced by paracetamol and CCl4[J].Pharm Res,1998,37(1):31.
[13] LIN W L,WANG C J,TSAL Y Y,et al.Inhibitory effect of esculetin on oxidative damage induced by t-butyl hydroperoxide in rat liver[J].Arch Toxicol,2000,74(8):467.
[14] 陈晓蕾,汤立建,李庆林,等.秦皮醇提取物体外抗乳腺癌细胞增殖的研究[J].中国药房,2007,18(15):1124.
[15] WANG C J,HSIEH Y J,CHU C Y,et al.Inhibition of cell cycle progression in human leukemia HL-60 cells by esculin[J].Cancer Letters,2002,183:163.
[16] KUO H C,LEE H J,HU C C,et al.Enhancement of esculetin on Taxol-induced apoptosis in human hepatoma HepG2 cells[J].Toxicology and Applied Pharmacology,2006,210(1/2):55-62.
[17] 伟忠民,王晶.秦皮乙素对人肝癌HepG2细胞体外增殖的影响及机制探讨[J].山东医药,2012,52(39):24-26.
[18] 梁敏.秦皮抗氧化成分的分离及其活性研究[J].食品工业科技,2006,27(3):64.
[19] 吴涛,仲婕,信学雷,等.毛菊苣种子质量标准研究[J].时珍国医国药,2010,21(3):705-707.