乌鸡肽的营养成分及其功能特性研究
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摘要
探讨乌鸡肽的营养成分及功能,为其深度开发打下基础。利用木瓜蛋白酶和风味蛋白酶水解乌骨鸡肌肉、活性炭脱色法制备乌鸡肽,高效液相色谱(high performance liquid chromatography,HPLC)法检测乌鸡肽的总氨基酸及游离氨基酸含量及相对分子质量分布区间、纳升级液相色谱串联质谱(Nano liquid chromatography tandem mass spectrometry,Nano LC-MS/MS)技术鉴定其组成成分。结果表明,游离氨基酸占总氨基酸的比例为1.74%,总氨基酸含量较高者为Leu、Ala、His、Pro、Met和Phe,游离氨基酸含量较高者为Glu、Pro、Asp、Arg、Gly和Ala;相对分子质量分布范围为0至6 918 Da,其中1 084 Da以下的比例达到88.91%;Nano LC-MS/MS鉴定出源于107种蛋白质的2 287种肽段序列,其中种类最为丰富的蛋白为参与骨骼肌修复的蛋白及酶类。该研究较为全面的分析乌鸡肽的营养成分,并表征了其功能,为乌鸡肽的潜在应用价值研究打下基础。
Active peptides of black-bone silky fowl(Gallus gallus domesticus Brisson,BSF) have an incomparable physiological and absorption function. BSF was hydrolyzed by complex enzymatic(flavor protease and papain)method and active carbon decoloration to obtain peptides. The molecular weight(MW)distribution and amino acid composition of the BSF hydrolysate(BSFH) were measured by a high performance liquid chromatography(HPLC)system. An analysis of functional properties for BSF peptides was performed by Nano LC-MS/MS. The results showed that the MW was from 0 to 6 918 Da,under 1 084 Da to 88.91 %;the major amino acids in total were Leu,Ala,His,Pro,Met and Phe;and the major amino acids in free were Glu,Pro,Asp,Arg,Gly and Ala;107 proteins were identified from 2 287 BSF peptides,which mainly took part in skeletal repair.
Active peptides of black-bone silky fowl(Gallus gallus domesticus Brisson,BSF) have an incomparable physiological and absorption function. BSF was hydrolyzed by complex enzymatic(flavor protease and papain)method and active carbon decoloration to obtain peptides. The molecular weight(MW)distribution and amino acid composition of the BSF hydrolysate(BSFH) were measured by a high performance liquid chromatography(HPLC)system. An analysis of functional properties for BSF peptides was performed by Nano LC-MS/MS. The results showed that the MW was from 0 to 6 918 Da,under 1 084 Da to 88.91 %;the major amino acids in total were Leu,Ala,His,Pro,Met and Phe;and the major amino acids in free were Glu,Pro,Asp,Arg,Gly and Ala;107 proteins were identified from 2 287 BSF peptides,which mainly took part in skeletal repair.
引文
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[2]Wei H,Yang R,Xie J.Nutrients of the black-bone chicken and its functions[J].Natural Product Research&Development,1995,7(3):85-91
[3]Liu J,Huang Y,Tian Y,et al.Purification and identification of novel antioxidative peptide released from Black-bone silky fowl(Gallus gallus domesticus Brisson)[J].European Food Research&Technology,2013,237(2):253-263
[4]Shen W,Matsui T.Current knowledge of intestinal absorption of bioactive peptides[J].Food&function,2017,8(12):4306-4314
[5]Kitts D D,Weiler K.Bioactive proteins and peptides from food sources.Applications of bioprocesses used in isolation and recovery[J].Current Pharmaceutical Design,2003,9(16):1309-1323
[6]Chen M,Li B.The effect of molecular weights on the survivability of casein-derived antioxidant peptides after the simulated gastrointestinal digestion[J].Innovative Food Science&Emerging Technologies,2012,16(39):341-348
[7]孙克岩,张志胜,佟海菊,等.响应面法优化木瓜蛋白酶和风味蛋白酶双酶酶解牡蛎工艺研究[J].食品科技,2011,36(6):13-17
[8]林波,于秀玲,王欣,等.双酶酶解制备羊骨多肽工艺研究[J].食品科技,2014,39(10):147-150
[9]张毅,华欲飞,孔祥珍,等.活性炭对大豆多肽脱色效果的研究[J].粮食与饲料工业,2010(8):31-33
[10]Liu J H,Tian Y G,Yong W,et al.Characterization and in vitro antioxidation of papain hydrolysate from black-bone silky fowl(Gallus gallus domesticus Brisson)muscle and its fractions[J].Food Research International,2011,44(1):133-138
[11]龙彪,彭志英,陈中,等.采用木瓜蛋白酶制备乌鸡蛋白肽的研究[J].食品工业科技,2005,26(6):135-137
[12]丁丹华,彭光华,何东平.HPLC法测定油茶籽多肽相对分子质量分布及氨基酸组成[J].中国油脂,2010,35(11):68-71
[13]罗浩铭,陈英红,洪铁,等.Nano LC-MS/MS从具有增强小鼠记忆作用的人参组分中鉴定22种新肽[J].天然产物研究与开发,2016,28(8):1223-1227
[14]Wang C,Wang B,Li B.Bioavailability of peptides from casein hydrolysate in vitro:Amino acid compositions of peptides affect the antioxidant efficacy and resistance to intestinal peptidases[J].Food Research International,2016,81:188-196
[15]刘奔,张熙凝,陈可欣.组蛋白赖氨酸甲基转移酶SET8对蛋白的甲基化修饰及与肿瘤相关性的研究进展[J].中国肿瘤临床,2015,42(15):765-769
[16]叶嘉良,张耀洲.泛素-核糖体蛋白S27a的克隆及表达研究[C].杭州:华东六省一市生物化学与分子生物学学会2006年学术交流会论文集,2006
[17]李湘竑,汪谦,罗燕,等.丝氨酸/苏氨酸蛋白激酶对肝癌细胞凋亡的促进作用[J].中华实验外科杂志,2006,23(1):55-56
[18]荣明,王春芳,石玉秀,等.电镜下纽蛋白在培养细胞分布及其影响因素[J].电子显微学报,2002,21(5):501-502