小尾寒羊FSHβ和LHβ基因在生殖轴的表达研究
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摘要
为揭示FSHβ和LHβ基因在小尾寒羊下丘脑-垂体-卵巢轴(HPOA)中的表达规律,深入了解其对小尾寒羊多羔的作用,本研究采用实时荧光定量PCR技术对6只小尾寒羊(FecB++型单、多羔羊各3只)的生殖组织及脑组织中FSHβ和LHβ基因的表达差异进行分析。结果表明:FSHβ和LHβ基因在大脑、小脑、下丘脑、卵巢、子宫、输卵管和垂体7种组织中均有表达,FSHβ主要在小尾寒羊下丘脑和卵巢高表达,LHβ在垂体高表达;FSHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体的表达极显著高于单羔群体(P<0.01),LHβ基因在小尾寒羊多羔群体下丘脑、卵巢、子宫、输卵管、垂体、小脑、大脑表达量均极显著高于单羔群体(P<0.01)。研究结果提示,FSHβ和LHβ基因可能参与小尾寒羊多羔性状调控。
In order to elucidate the expression patterns of FSHβ and LHβ genes in the hypothalamic-pituitary-ovarian axis(HPOA), and to reveal the mechanism of prolificacy in Small Tail Han Sheep. In this study, real-time fluorescence quantitative PCR(RT-qPCR) was used to detect the expression of the FSHβ and LHβ genes in reproductive tissues and brain tissues of six Small Tail Han Sheep(three polytocous and three monotocous ewes from the FecB ++ genotype). The results showed that FSHβ and LHβ genes were expressed in brain, cerebellum, hypothalamus, ovary, uterus, oviduct and pituitary tissues. Among them, FSHβ was highly expressed in hypothalamus and ovary, LHβ was highly expressed in pituitary. The expression of FSHβ gene in hypothalamus, ovary, uterus, oviduct and pituitary, LHβ gene in hypothalamus, ovary, uterus, oviduct, pituitary, cerebellum and brain of polytocous Small Tail Han ewes was extremely significant higher(P<0.01) than that of monotocous ewes. The results suggested that FSHβ and LHβ genes may be involved in the regulation of fecundity in sheep.us might be considered as a potential molecular marker which controls seasonal reproduction and litter size in sheep.
In order to elucidate the expression patterns of FSHβ and LHβ genes in the hypothalamic-pituitary-ovarian axis(HPOA), and to reveal the mechanism of prolificacy in Small Tail Han Sheep. In this study, real-time fluorescence quantitative PCR(RT-qPCR) was used to detect the expression of the FSHβ and LHβ genes in reproductive tissues and brain tissues of six Small Tail Han Sheep(three polytocous and three monotocous ewes from the FecB ++ genotype). The results showed that FSHβ and LHβ genes were expressed in brain, cerebellum, hypothalamus, ovary, uterus, oviduct and pituitary tissues. Among them, FSHβ was highly expressed in hypothalamus and ovary, LHβ was highly expressed in pituitary. The expression of FSHβ gene in hypothalamus, ovary, uterus, oviduct and pituitary, LHβ gene in hypothalamus, ovary, uterus, oviduct, pituitary, cerebellum and brain of polytocous Small Tail Han ewes was extremely significant higher(P<0.01) than that of monotocous ewes. The results suggested that FSHβ and LHβ genes may be involved in the regulation of fecundity in sheep.us might be considered as a potential molecular marker which controls seasonal reproduction and litter size in sheep.
引文
[1]张兴国,徐相亭,寇鑫.寒细杂交肉羊高繁殖力基因研究[J].中国动物保健,2017,19(9):90-91.
[2]Marshall J C,Kelch R P.Gonadotropin-releasing hormone:role of pulsatile secretion in the regulation of reproduction[J].New Engl J Med,1986,315:1468-1469.
[3]吴舒洁,储明星,过玮,等.FSHβ基因5'调控区多态性与小尾寒羊产羔数的关系[J].扬州大学学报(农业与生命科学版),2013,34(3):33-36.
[4]葛莹,邓峥,宋春雷,等.促黄体素β基因多态性与小尾寒羊产羔数相关性分析[J].扬州大学学报(农业与生命科学版),2015,36(1):32-37.
[5]刘建斌,郎侠,杨博辉,等.BMPR-IB基因和FSHβ基因多态性及其与小尾寒羊产羔数关联性分析[J].中国畜牧杂志,2010,46(19):1-6.
[6]Zi X D,Huang L,Wang Y,et al.Comparative messenger mRNAexpression of FSHβ,LHβ,FSHR,LHR,and ERβin high and low prolific goat breeds[J].Anim Biotechol,2013,24:307-311.
[7]Kumar T R.Functional analysis of LH beta knock-out mice[J].Mol Cell Endocrinal,2007,269(1-2):81-84.
[8]Nivet A L,Vigneault C,Blondin P,et al.Influence of luteinizing hormone support on granulosa cells transcriptome in cattle[J].Anim Sci J,2018,89(1):21-30.
[9]Thomas F H,Vanderhyden B C.Oocyte granulosa cell interactions during mouse follicular development:regulation of kit ligand expression and its role in oocyte growth[J].Reprod Biol Endocrinol,2003,1:1-7.
[10]Wei S,Gong Z,Guo H,et al.FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice[J].Iran J Vet Res,2017,18(2):79.
[11]Suocheng W,Zhuandi G,Li S,et al.Maturation rates of oocytes and levels of FSHR,LHR and GnRHR of COCs response to FSHconcentrations in IVM media for sheep[J].J Appl Biomed,2017,15(3):180-186.
[12]杜红丽,陈静,张玉山,等.二花脸与杜洛克猪繁殖相关基因表达差异[J].华南农业大学学报,2008(2):99-103.
[13]石福岳,孙露露,秦立志,等.促卵泡激素β基因在家兔不同生殖器官中的差异表达研究[J].中国畜牧杂志,2013,49(15):23-26.
[14]马国辅,高利华,李定国,等.FSHβ与FSHR基因在小梅山猪不同生长阶段表达规律的研究[J].黑龙江畜牧兽医,2014(13):21-24,27.
[15]魏岳,武艳平,刘林秀,等.不同光照条件对兴国灰鹅FSHβ、PRL和VIP基因m RNA表达水平的影响[J].江西农业大学学报,2016,38(5):947-953.
[16]产舒恒,陈子璇,张戌园,等.FSH和LH及其受体基因在梅山猪公猪HPT轴的表达[J].江苏农业科学,2018,46(1):23-26.
[17]吴国平.浙东白鹅繁殖周期中PRL、FSH和LH基因的表达分析[D].北京:中国农业科学院,2014.
[18]史军红,何玉琴,葛文博,等.高原甘加藏羊发情周期垂体FSHβ和LHβm RNA表达差异的研究[J].中国兽医科学,2016,46(11):1444-1449.
[2]Marshall J C,Kelch R P.Gonadotropin-releasing hormone:role of pulsatile secretion in the regulation of reproduction[J].New Engl J Med,1986,315:1468-1469.
[3]吴舒洁,储明星,过玮,等.FSHβ基因5'调控区多态性与小尾寒羊产羔数的关系[J].扬州大学学报(农业与生命科学版),2013,34(3):33-36.
[4]葛莹,邓峥,宋春雷,等.促黄体素β基因多态性与小尾寒羊产羔数相关性分析[J].扬州大学学报(农业与生命科学版),2015,36(1):32-37.
[5]刘建斌,郎侠,杨博辉,等.BMPR-IB基因和FSHβ基因多态性及其与小尾寒羊产羔数关联性分析[J].中国畜牧杂志,2010,46(19):1-6.
[6]Zi X D,Huang L,Wang Y,et al.Comparative messenger mRNAexpression of FSHβ,LHβ,FSHR,LHR,and ERβin high and low prolific goat breeds[J].Anim Biotechol,2013,24:307-311.
[7]Kumar T R.Functional analysis of LH beta knock-out mice[J].Mol Cell Endocrinal,2007,269(1-2):81-84.
[8]Nivet A L,Vigneault C,Blondin P,et al.Influence of luteinizing hormone support on granulosa cells transcriptome in cattle[J].Anim Sci J,2018,89(1):21-30.
[9]Thomas F H,Vanderhyden B C.Oocyte granulosa cell interactions during mouse follicular development:regulation of kit ligand expression and its role in oocyte growth[J].Reprod Biol Endocrinol,2003,1:1-7.
[10]Wei S,Gong Z,Guo H,et al.FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice[J].Iran J Vet Res,2017,18(2):79.
[11]Suocheng W,Zhuandi G,Li S,et al.Maturation rates of oocytes and levels of FSHR,LHR and GnRHR of COCs response to FSHconcentrations in IVM media for sheep[J].J Appl Biomed,2017,15(3):180-186.
[12]杜红丽,陈静,张玉山,等.二花脸与杜洛克猪繁殖相关基因表达差异[J].华南农业大学学报,2008(2):99-103.
[13]石福岳,孙露露,秦立志,等.促卵泡激素β基因在家兔不同生殖器官中的差异表达研究[J].中国畜牧杂志,2013,49(15):23-26.
[14]马国辅,高利华,李定国,等.FSHβ与FSHR基因在小梅山猪不同生长阶段表达规律的研究[J].黑龙江畜牧兽医,2014(13):21-24,27.
[15]魏岳,武艳平,刘林秀,等.不同光照条件对兴国灰鹅FSHβ、PRL和VIP基因m RNA表达水平的影响[J].江西农业大学学报,2016,38(5):947-953.
[16]产舒恒,陈子璇,张戌园,等.FSH和LH及其受体基因在梅山猪公猪HPT轴的表达[J].江苏农业科学,2018,46(1):23-26.
[17]吴国平.浙东白鹅繁殖周期中PRL、FSH和LH基因的表达分析[D].北京:中国农业科学院,2014.
[18]史军红,何玉琴,葛文博,等.高原甘加藏羊发情周期垂体FSHβ和LHβm RNA表达差异的研究[J].中国兽医科学,2016,46(11):1444-1449.