NaOH、HCl、BaCl_2对家兔小肠段平滑肌收缩的影响
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摘要
平滑肌对一些酸碱等化学物质较为敏感,其可能会影响小肠的肌电活动,进而影响小肠的运动。本研究试图探讨氢氧化钠(Na OH)、盐酸(HCl)、氯化钡(Ba Cl_2)对离体家兔小肠段平滑肌收缩功能的影响,以期了解不同化学药物对小肠肌电活动的影响,为肠道疾病的诊断和治疗提供参考。本研究选取健康家兔,处死后取家兔的十二指肠、空肠、回肠离体后进行恒温灌流,分别给予1 mol/L的Na OH溶液、1 mol/LHCl溶液、1 mol/L Ba Cl_2溶液,并选择相对应肠段作为对照组,仅给予等量生理盐水恒温灌流,对比各组小肠平滑肌电活动变化。研究表明,Ba Cl_2干预下,十二指肠、空肠、回肠的收缩频率均高于对照组、Na OH组、HCl组,差异均具有统计学意义(p<0.05);Ba Cl_2组、Na OH组、HCl组离体家兔小肠平滑肌的最大振幅均显著的高于对照组(p<0.05),Ba Cl_2组离体家兔小肠平滑肌的最大振幅均显著的高于Na OH组、HCl组(p<0.05);Ba Cl_2组、Na OH组离体家兔小肠平滑肌的负波最大振幅均显著的高于对照组、HCl组(p<0.05),Ba Cl_2组离体家兔小肠平滑肌的负波最大振幅均显著的高于Na OH组(p<0.05)。我们的研究初步表明:Ba Cl_2能显著的增强离体家兔小肠段平滑肌收缩功能,Na OH和HCl溶液会增强体家兔小肠平滑肌的最大振幅。研究显示Ba Cl_2的作用机制可能是促使平滑肌强直性收缩,而Na OH和HCl酸碱类物质的刺激可使肠壁内神经元兴奋,促进小肠平滑肌的运动。
Smooth muscle is sensitive to some chemicals,such as acids and bases,which may affect the myoelectric activity of the small intestine,thereby affecting the movement of the small intestine.This study attempted to explore the effect of Na OH,HCl and Ba Cl_2 on contraction of smooth muscle in isolated small intestine of rabbits,in order to understand the effects of chemical drugs on intestinal myoelectric activity,so as to provide the reference for the diagnosis and treatment of intestinal diseases.In this study,healthy rabbits were selected and put to death.Their duodenum,jejunum and ileum were taken out to receive constant temperature perfusion with 1 mol/L Na OH solution,1 mol/L HCl solution,and 1 mol/L Ba Cl_2 solution,respectively.Also,the corresponding intestinal segments were chosen as the control group and only received the same amount of saline perfusion.Then,the electrical activity changes of small intestinal smooth muscle were compared.The results showed that under the intervention of Ba Cl_2,the contraction frequencies of duodenum,jejunum and ileum were all higher than those of control group,group Na OH and group HCl,and the difference was statistically significant(p<0.05).The maximum amplitude of isolated small intestinal smooth muscle in rabbit of Ba Cl_2 group,Na OH group and HCl group was obviously higher than that in control group(p<0.05).In addition,the maximal amplitude of small intestine smooth muscle of Ba Cl_2 group was significantly higher than that of Na OH group and HCl group(p<0.05).The maximal amplitude of the negative wave in the small intestine smooth muscle of Ba Cl_2 group was significantly higher than that of the control group and the HCl group(p<0.05),and the maximal amplitude of negative wave in the small intestine smooth muscle of Ba Cl_2 group was apparently higher than that of Na OH group(p<0.05).Our study preliminarily demonstrated that Ba Cl_2 could significantly enhance the contraction function of isolated small intestine smooth muscle in rabbits,and Na OH and HCl solution could increase the maximum amplitude of small intestine smooth muscle of rabbits.Moreover,this research indicated that the mechanism of action of Ba Cl_2 might be to promote the tetanic contraction of smooth muscle,while the stimulation of Na OH and HCl acids and bases could stimulate neurons in intestinal wall and promote the movement of small intestinal smooth muscle.
Smooth muscle is sensitive to some chemicals,such as acids and bases,which may affect the myoelectric activity of the small intestine,thereby affecting the movement of the small intestine.This study attempted to explore the effect of Na OH,HCl and Ba Cl_2 on contraction of smooth muscle in isolated small intestine of rabbits,in order to understand the effects of chemical drugs on intestinal myoelectric activity,so as to provide the reference for the diagnosis and treatment of intestinal diseases.In this study,healthy rabbits were selected and put to death.Their duodenum,jejunum and ileum were taken out to receive constant temperature perfusion with 1 mol/L Na OH solution,1 mol/L HCl solution,and 1 mol/L Ba Cl_2 solution,respectively.Also,the corresponding intestinal segments were chosen as the control group and only received the same amount of saline perfusion.Then,the electrical activity changes of small intestinal smooth muscle were compared.The results showed that under the intervention of Ba Cl_2,the contraction frequencies of duodenum,jejunum and ileum were all higher than those of control group,group Na OH and group HCl,and the difference was statistically significant(p<0.05).The maximum amplitude of isolated small intestinal smooth muscle in rabbit of Ba Cl_2 group,Na OH group and HCl group was obviously higher than that in control group(p<0.05).In addition,the maximal amplitude of small intestine smooth muscle of Ba Cl_2 group was significantly higher than that of Na OH group and HCl group(p<0.05).The maximal amplitude of the negative wave in the small intestine smooth muscle of Ba Cl_2 group was significantly higher than that of the control group and the HCl group(p<0.05),and the maximal amplitude of negative wave in the small intestine smooth muscle of Ba Cl_2 group was apparently higher than that of Na OH group(p<0.05).Our study preliminarily demonstrated that Ba Cl_2 could significantly enhance the contraction function of isolated small intestine smooth muscle in rabbits,and Na OH and HCl solution could increase the maximum amplitude of small intestine smooth muscle of rabbits.Moreover,this research indicated that the mechanism of action of Ba Cl_2 might be to promote the tetanic contraction of smooth muscle,while the stimulation of Na OH and HCl acids and bases could stimulate neurons in intestinal wall and promote the movement of small intestinal smooth muscle.
引文
Gajendiran V.,and Buist M.L.,2015,A quantitative description of active force generation in gastrointestinal smooth muscle,Int.J.Numer.Method.Biomed.Eng.,27(3):450-460
He W.,Zhu L.,Fan X.A.,Yang S.Y.,Liu R.N.,and Chen L.,2016,Effects of ascending to high altitude on ultrastructure of enteric nervous system-ICC-smooth muscle cells network in gastric electrical pacing region of rats,Shanxi Yike Daxue Xuebao(Journal of Shanxi Medical University),47(12):1061-1066(贺巍,朱琳,范兴爱,杨生岳,刘睿年,陈磊,2016,急进高原胃动力紊乱大鼠胃电起搏区肠神经系统-Cajal间质细胞-消化道平滑肌网络微观结构及功能的变化,山西医科大学学报,47(12):1061-1066)
Kalibixiati A.M.L.J.,Zhou Y.D.,Yan S.P.,Halidan A.H.M.T.,and Zhao H.Q.,2016,Effects of temperature changes on the contractility of isolated small intestinal smooth muscle in rabbits,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(8):2060-2065(卡力比夏提·艾木拉江,周玉东,闫书平,哈力旦阿合买提,赵红琼,2016,温度变化对家兔离体小肠平滑肌运动的影响,中国畜牧兽医,43(8):2060-2065)
Krishna C.V.,Singh J.,Thangavel C.,and Rattan S.,2016,Role of micro RNAs in gastrointestinal smooth muscle fibrosis and dysfunction:novel molecular perspectives on the pathophysiology and therapeutic targeting,Am.J.Physiol.Gastrointest.Liver Physiol.,310(7):G449-G459
Park C.,Lee M.Y.,Park P.J.,Ha S.E.,Berent R.M.,Fuchs R.,Miano J.M.,Becker L.S.,Sanders K.M.,and Ro S.,2015,Serum response factor is essential for prenatal gastrointestinal smooth muscle development and maintenance of differentiated phenotype,J.Neurogastroenterol.Motil.,21(4):589-602
Peng W.J.,Xin R.H.,Liu Y.,Luo Y.J.,Wang G.B.,Luo C.Y.,Xie J.S.,Li J.Y.,and Zheng J.F.,2016,Effects of alcohol extract of aster tataricus L.f.on the contraction of guinea pig tracheal smooth muscle in vitro,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(6):1572-1578(彭文静,辛蕊华,刘艳,罗永江,王贵波,罗超应,谢家声,李锦宇,郑继方,2016,紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响,中国畜牧兽医,43(6):1572-1578)
Scirocco A.,Matarrese P.,Carabotti M.,Ascione B.,Malorni W.,and Severi C.,2016,Cellular and molecular mechanisms of phenotypic switch in gastrointestinal smooth muscle,J.Cell Physiol.,231(2):295-302
Shen J.M.,Miao S.B.,Li X.Y.,Cui F.H.,Li Y.,Li N.,Liu C.G.,Jin R.Z.,and Guan Y.,2016,Effect of dexmedetomidine on spontaneous contraction of colon smooth muscles of rabbits in vitro,Zhonghua Mazuixue Zazhi(Chinese Journal of Anesthesiology),36(4):442-446(申军梅,苗穗兵,李旭懿,崔芳华,李煜,李楠,刘宸光,靳睿哲,关玥,2016,右美托咪定对家兔结肠平滑肌自发收缩功能的影响:离体实验,中华麻醉学杂志,36(4):442-446)
Wang G.Y.,Wang H.Y.,and Wang L.,2015,Relationship between effect of WEPO on tracheal smooth muscleβand Mreceptors,Junshi Yixue(Military Medical Sciences),39(9):688-690(王国玉,王浩宇,王璐,2015,马齿苋水提取物平喘作用与β受体和M受体的关系,军事医学,39(9):688-690)
Xiang D.B.,Yang Y.,Liu X.J.,and Shi S.C.,2015,Study on influences of ethanol extract from Euphorbia humifusa(EEEH)on smooth muscle of isolated small intestine in rabbits and its mechanism,Zhongguo Yiyuan Yaoxue Zazhi(Chinese Journal of Hospital Pharmacy),35(15):1371-1375(向德标,阳漾,刘小娟,石胜超,2015,地锦草醇提物对兔离体小肠平滑肌作用及机制研究,中国医院药学杂志,35(15):1371-1375)
Yang H.L.,Duan Y.H.,and Qian X.Y.,2017,Effects of coptis decoction in various doses on gastrointestinal motor function of normal and spleen deficiency rats,Xiandai Zhongxiyi Jiehe Zazhi(Modern Journal of Integrated Traditional Chinese and Western Medicine),26(1):14-17(杨红莲,段玉红,钱笑音,2017,不同剂量黄连水煎液对正常和脾虚大鼠胃肠运动功能的影响,现代中西医结合杂志,26(1):14-17)
Zhang D.,Shi G.,Zhou Y.D.,Yan J.,Wang L.F.,Meng K.J.,Guo J.,and Zhao H.Q.,2016,Effect of alcohol extract from different parts of sophoraalopecuroides on the motility of isolated rat small intestine,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(1):164-170(张丹,石刚,周玉东,闫静,王丽芳,孟坤杰,郭晶,赵红琼,2016,苦豆子植株不同部位醇提液对大鼠离体小肠运动的影响,中国畜牧兽医,43(1):164-170)
He W.,Zhu L.,Fan X.A.,Yang S.Y.,Liu R.N.,and Chen L.,2016,Effects of ascending to high altitude on ultrastructure of enteric nervous system-ICC-smooth muscle cells network in gastric electrical pacing region of rats,Shanxi Yike Daxue Xuebao(Journal of Shanxi Medical University),47(12):1061-1066(贺巍,朱琳,范兴爱,杨生岳,刘睿年,陈磊,2016,急进高原胃动力紊乱大鼠胃电起搏区肠神经系统-Cajal间质细胞-消化道平滑肌网络微观结构及功能的变化,山西医科大学学报,47(12):1061-1066)
Kalibixiati A.M.L.J.,Zhou Y.D.,Yan S.P.,Halidan A.H.M.T.,and Zhao H.Q.,2016,Effects of temperature changes on the contractility of isolated small intestinal smooth muscle in rabbits,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(8):2060-2065(卡力比夏提·艾木拉江,周玉东,闫书平,哈力旦阿合买提,赵红琼,2016,温度变化对家兔离体小肠平滑肌运动的影响,中国畜牧兽医,43(8):2060-2065)
Krishna C.V.,Singh J.,Thangavel C.,and Rattan S.,2016,Role of micro RNAs in gastrointestinal smooth muscle fibrosis and dysfunction:novel molecular perspectives on the pathophysiology and therapeutic targeting,Am.J.Physiol.Gastrointest.Liver Physiol.,310(7):G449-G459
Park C.,Lee M.Y.,Park P.J.,Ha S.E.,Berent R.M.,Fuchs R.,Miano J.M.,Becker L.S.,Sanders K.M.,and Ro S.,2015,Serum response factor is essential for prenatal gastrointestinal smooth muscle development and maintenance of differentiated phenotype,J.Neurogastroenterol.Motil.,21(4):589-602
Peng W.J.,Xin R.H.,Liu Y.,Luo Y.J.,Wang G.B.,Luo C.Y.,Xie J.S.,Li J.Y.,and Zheng J.F.,2016,Effects of alcohol extract of aster tataricus L.f.on the contraction of guinea pig tracheal smooth muscle in vitro,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(6):1572-1578(彭文静,辛蕊华,刘艳,罗永江,王贵波,罗超应,谢家声,李锦宇,郑继方,2016,紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响,中国畜牧兽医,43(6):1572-1578)
Scirocco A.,Matarrese P.,Carabotti M.,Ascione B.,Malorni W.,and Severi C.,2016,Cellular and molecular mechanisms of phenotypic switch in gastrointestinal smooth muscle,J.Cell Physiol.,231(2):295-302
Shen J.M.,Miao S.B.,Li X.Y.,Cui F.H.,Li Y.,Li N.,Liu C.G.,Jin R.Z.,and Guan Y.,2016,Effect of dexmedetomidine on spontaneous contraction of colon smooth muscles of rabbits in vitro,Zhonghua Mazuixue Zazhi(Chinese Journal of Anesthesiology),36(4):442-446(申军梅,苗穗兵,李旭懿,崔芳华,李煜,李楠,刘宸光,靳睿哲,关玥,2016,右美托咪定对家兔结肠平滑肌自发收缩功能的影响:离体实验,中华麻醉学杂志,36(4):442-446)
Wang G.Y.,Wang H.Y.,and Wang L.,2015,Relationship between effect of WEPO on tracheal smooth muscleβand Mreceptors,Junshi Yixue(Military Medical Sciences),39(9):688-690(王国玉,王浩宇,王璐,2015,马齿苋水提取物平喘作用与β受体和M受体的关系,军事医学,39(9):688-690)
Xiang D.B.,Yang Y.,Liu X.J.,and Shi S.C.,2015,Study on influences of ethanol extract from Euphorbia humifusa(EEEH)on smooth muscle of isolated small intestine in rabbits and its mechanism,Zhongguo Yiyuan Yaoxue Zazhi(Chinese Journal of Hospital Pharmacy),35(15):1371-1375(向德标,阳漾,刘小娟,石胜超,2015,地锦草醇提物对兔离体小肠平滑肌作用及机制研究,中国医院药学杂志,35(15):1371-1375)
Yang H.L.,Duan Y.H.,and Qian X.Y.,2017,Effects of coptis decoction in various doses on gastrointestinal motor function of normal and spleen deficiency rats,Xiandai Zhongxiyi Jiehe Zazhi(Modern Journal of Integrated Traditional Chinese and Western Medicine),26(1):14-17(杨红莲,段玉红,钱笑音,2017,不同剂量黄连水煎液对正常和脾虚大鼠胃肠运动功能的影响,现代中西医结合杂志,26(1):14-17)
Zhang D.,Shi G.,Zhou Y.D.,Yan J.,Wang L.F.,Meng K.J.,Guo J.,and Zhao H.Q.,2016,Effect of alcohol extract from different parts of sophoraalopecuroides on the motility of isolated rat small intestine,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),43(1):164-170(张丹,石刚,周玉东,闫静,王丽芳,孟坤杰,郭晶,赵红琼,2016,苦豆子植株不同部位醇提液对大鼠离体小肠运动的影响,中国畜牧兽医,43(1):164-170)