油瓜的化学成分研究
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摘要
研究了油瓜(Hodgsonia macrocarpa)全株植物中的化学成分.采用硅胶、Sephadex LH-20、HPLC、C18等色谱方法对油瓜中的化合物进行分离纯化,应用核磁及质谱技术鉴定化合物结构.从油瓜的根茎叶中分离得到了5个化合物,大黄酚(1)、大黄素甲醚(2)、2,6-Dimethoxy-4-methylphenol(3)、蔗糖(4)、葡萄糖(5),用自由基清除率实验检测化合物的氧化性.化合物1～3为首次从该植物中分离得到,其中化合物2和3具有一定的抗氧化活性.
This research aims to study the chemical components of Hodgsonia macrocarpa. It adopts some chromatographic techniques like Sephadex LH-20 and C18-HPLC to isolate and purify the compounds and the spectral methods to elucidate their structures. Five compounds have been isolated from the roots and stems of H. macrocarpa: Chrysophanol(1), chrysophanol methyl ether(2), 2,6-Dimethoxy-4-methylphenol(3), sucrose(4), and glucose(5). The anti-oxidant activity of the compounds is detected by radical scavenging experiments. Among them,(1),(2) and(3)are extracted from H. macrocarpa for the first time, while chrysophanol methyl ether and 2,6-Dimethoxy-4-methylph enol showed some antioxidant activity.
This research aims to study the chemical components of Hodgsonia macrocarpa. It adopts some chromatographic techniques like Sephadex LH-20 and C18-HPLC to isolate and purify the compounds and the spectral methods to elucidate their structures. Five compounds have been isolated from the roots and stems of H. macrocarpa: Chrysophanol(1), chrysophanol methyl ether(2), 2,6-Dimethoxy-4-methylphenol(3), sucrose(4), and glucose(5). The anti-oxidant activity of the compounds is detected by radical scavenging experiments. Among them,(1),(2) and(3)are extracted from H. macrocarpa for the first time, while chrysophanol methyl ether and 2,6-Dimethoxy-4-methylph enol showed some antioxidant activity.
引文
[1] 邱明华,陈书坤,陈剑超,等.葫芦科化学分类学[J].应用与环境生物学报,2005,11(6):673-685.
[2] 蔡希陶.油瓜的生物学特性[J].生物学通报,1962(3):3-5.
[3] 廖学焜,郭慧然.油瓜油的甘油三酯组成研究[J].广西植物,1988(3):82-84.
[4] 孟博,龙春林,程治英,等.生物柴油原料植物油瓜的开发利用[J].北方园艺,2007(10):51-54.
[5] 王趁,张玲玲,王雨华,等.油瓜的民族植物学研究[J].植物资源与环境学报,2015,37(2):209-213.
[6] 白少娟,田玉欣,王炎,等.北青龙衣氯仿部位化学成分的分离与鉴定[J].中国药物化学杂志,2017,27(01):52-56.
[7] 戴鸥,仰莲,周勤梅,等.白及化学成分分离鉴定[J].中国实验方剂学杂志,2018,24(14):43-47.
[8] TANG R,WEBSTER F X,MüLLER-SCHWARZE D.Neutral compounds from male castoreum of North American beaver,Castor canadensis[J].Journal of Chemical Ecology,1995,21(11):1745-1762.
[9] 杨林.瑞香狼毒茎部乙酸乙酯提取物化学成分的研究[D].呼和浩特:内蒙古大学,2012.
[10] 吕本强.注射用双黄连粉针剂物质基础研究[D].广州:第一军医大学,2007.
[2] 蔡希陶.油瓜的生物学特性[J].生物学通报,1962(3):3-5.
[3] 廖学焜,郭慧然.油瓜油的甘油三酯组成研究[J].广西植物,1988(3):82-84.
[4] 孟博,龙春林,程治英,等.生物柴油原料植物油瓜的开发利用[J].北方园艺,2007(10):51-54.
[5] 王趁,张玲玲,王雨华,等.油瓜的民族植物学研究[J].植物资源与环境学报,2015,37(2):209-213.
[6] 白少娟,田玉欣,王炎,等.北青龙衣氯仿部位化学成分的分离与鉴定[J].中国药物化学杂志,2017,27(01):52-56.
[7] 戴鸥,仰莲,周勤梅,等.白及化学成分分离鉴定[J].中国实验方剂学杂志,2018,24(14):43-47.
[8] TANG R,WEBSTER F X,MüLLER-SCHWARZE D.Neutral compounds from male castoreum of North American beaver,Castor canadensis[J].Journal of Chemical Ecology,1995,21(11):1745-1762.
[9] 杨林.瑞香狼毒茎部乙酸乙酯提取物化学成分的研究[D].呼和浩特:内蒙古大学,2012.
[10] 吕本强.注射用双黄连粉针剂物质基础研究[D].广州:第一军医大学,2007.