摘要
自小麦赤霉病发病田块土壤中,分离得到1株小麦赤霉病高效拮抗菌株。通过形态特征、生理生化特性和16S rDNA序列比对分析,将这株拮抗菌株鉴定为奇异变形杆菌(proteus mirabilis)。P. mirabilis DY05发酵液和无细胞上清均可显著抑制禾谷镰刀菌菌丝体生长(抑制率分别为79.50%和51.25%)和分生孢子萌发(抑制率均为100%),减少呕吐毒素产生(分别减少84.32%和82.82%)。田间赤霉病防治试验结果显示,接种DY05,可降低发病率52.13%,同时病情指数降低48.74%,显示出较好的防治效果。促生生理活性评价试验结果显示,菌株DY05可以产生铁载体和IAA,并具有溶磷作用和ACC脱氨酶活性,具有很好的促生长潜力。盆栽试验结果表明,菌株DY05对小麦植株生长具有显著的促进作用。与对照组相比,菌株DY05处理可以显著增加小麦幼苗茎高、根长、鲜重和干重,其中茎高、根长、鲜重和干重分别提高了22.21%、26.41%、44.77%和26.53%。分离得到的拮抗菌株DY05具有拮抗病原真菌和促进植物生长的双重功能,为开发禾谷镰刀菌生物防治制剂提供了菌种材料。
One bacterial strain against Fusarium graminearum was isolated from a cultivated soil and identified as Proteus mirabilis basing on morphologic observation, biochemical characteristic analysis and 16 S rDNA sequences alignment. Both the fermentation broth and the cell-free supernatant of P. mirabilis DY05 could significantly inhibit the hyphal growth of F.graminearum(with the inhibition rate 79.50% and 51.25% respectively) and conidiospore germination of F. graminearum(both inhibition rates were 100%), as well as reduce DON production in wheat(decreased by 84.32% and 82.82%respectively). Field assays showed a significant reduction of Fusarium head blight(FHB) diseases on wheat when DY05 suspension was sprayed on wheat spikes after inoculation with F. graminearum(incidence rate decreased by 52.13%, while the disease index decreased by 48.74%). The modified CAS assay, the Salkowski colorimetry, molybdenum blue method and ACC deaminase assay were used to measure production of siderophores, HCN, IAA, phosphate solubilizing capacity and ACC deaminase in the strain DY05. The results indicated that strain DY05 could not only produce siderophores, IAA and ACC deaminase, but also solubilize phosphate. Moreover, in pot plant experiments, the strain DY05 showed significant growth promoting effects on the stem height, root length, fresh weight and dry weight of wheat seedlings(increased by22.21%, 26.41%, 44.77% and 26.53% respectively). Overall, the antagonistic strain DY05 was found to antagonize F.graminearum and showed a high plant growth promotion, and were therefore could offer available resource for development of new biocontrol agents.
引文
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