饲料中添加硒化壳聚糖对大鳞副泥鳅抗氧化能力的影响
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摘要
以体质量为4.9±0.2 g的泥鳅为实验对象,在基础饲料中添加了不同含量(0%、0.3%、0.6%、0.9%和1.2%)的硒化壳聚糖。经过为期60 d的养殖后,比较实验鳅肝脏中过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性,以及丙二醛含量和总抗氧化能力的变化。结果发现:硒化壳聚糖对泥鳅过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)活性有明显的促进作用,CAT活性随着硒化壳聚糖含量增加而增大,在添加量为1.2%时活性最大,各实验组的GPX活性均显著高于空白组;超氧化物歧化酶(SOD)的活性在硒化壳聚糖添加水平大于0.3%时显著高于对照组,并在1.2%添加水平达到最大;实验组泥鳅肝脏内丙二醛(MDA)含量相比于空白组明显下降,且随着硒化壳聚糖的浓度提升而下降,在1.2%时达到最低;实验组总抗氧化能力也有明显的上升,在0.9%时达到最大。整体上,饲料中添加硒化壳聚糖可以显著提升大鳞副泥鳅的抗氧化能力。
A 60-day feeding trial was conducted to determine the effect of seleno-chitosan supplements on antioxidant ability of juvenile loach(initial weight of 4.9±0.2 g) reared in experimental aquaria.Five test diets containing 0%,0.3%,0.6%,0.9% and 1.2% seleno-chitosan were randomly assigned to 15 aquaria,respectively.At the end of the experiment,superoxide dismutase,catalase and glutathione peroxidase activities were significantly elevated with concomitant increasing levels of seleno-chitosan(P<0.05),especially when the additive level was above 0.9%.While malondialdehyde content decreased with the addition of seleno-chitosan.These results indicate that seleno-chitosan could improve antioxidant ability of juvenile loach.
A 60-day feeding trial was conducted to determine the effect of seleno-chitosan supplements on antioxidant ability of juvenile loach(initial weight of 4.9±0.2 g) reared in experimental aquaria.Five test diets containing 0%,0.3%,0.6%,0.9% and 1.2% seleno-chitosan were randomly assigned to 15 aquaria,respectively.At the end of the experiment,superoxide dismutase,catalase and glutathione peroxidase activities were significantly elevated with concomitant increasing levels of seleno-chitosan(P<0.05),especially when the additive level was above 0.9%.While malondialdehyde content decreased with the addition of seleno-chitosan.These results indicate that seleno-chitosan could improve antioxidant ability of juvenile loach.
引文
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[30]杨凡,屈亮,聂琴.酵母硒在水生动物上的应用研究[J].饲料研究,2013(10):36-38.
[31]曹丹,周洪亮.不同添加剂对暗纹东方魨生长和脾溶菌酶活力的影响[J].饲料广角,2002(11):18-19.
[32]常仁亮,韩保平,顾润润.硒酵母的培养及其养虾效果[J].水产学报,2000,24(5):458-462.
[33]王井亮,叶亮宏,周明.硒源和硒水平对克氏螯虾肉品质的影响[J].湖南饲料,2010(3):24-26.
[34]徐有卿,李太元,丁兆坤,等.添加酵母硒对鱤鱼消化酶活性鱼饲料转化率的影响[J].水产科学,2013,32(7):391-395.
[35]吕晨辉.富硒益生菌对高温条件下仔猪生产性能、抗氧化能力和肠道菌群的影响[D].南京:南京农业大学,2013.
[2]YANG Y R,MENG F C,WANG P,et al.Effect of organic and inorganic selenium supplementation on growth performance,meat quality and antioxidant property of broilers[J].African Journal of Biotechnology,2014,11(12):3 031-3 036.
[3]IBRAHIM M T,ELJACK B H,FADLALLA I M T.Selenium supplementation to broiler diets[J].Animal Science Journal,2011,2(1):12-17.
[4]TAKAHASHI L S,BILLER-TAKAHASHI J D,MANSANO C F M,et al.Long-term organic selenium supplementation overcomes the trade-off between immune and antioxidant systems in pacu(Piaractus mesopotamicus)[J].Fish&Shellfish Immunology,2107,60:311-317.
[5]HOFFMANN P R.Mechanisms by which selenium influences immune responses[J].Arch Immunol Ther,2007,55:289-297.
[6]陈里,詹逸舒,吴永尧.富硒酵母培养条件的优化[J].湖南农业大学学报,2009,35(3):237-241.
[7]ANAS A,PAUL S,JAYAPRAKASH N S,et al.Antimicrobial activity of chitosan against vibrios from freshwater prawn Macrobrachium rosenbergii larval rearing systems[J].Diseases of Aquatic Organisms,2005,67(1/2):177-179.
[8]KAMALI N M,IMANPOOR M R,TAGHIZADEH V,et al.Effect of dietary chitosan on growth performance,hematological parameters,intestinal histology and stress resistance of Caspian kutum(Rutilus frisii kutum Kamenskii,1901)fingerlings[J].Fish Physiology and Biochemistry,2016,42(4):1 063-1 071.
[9]DANANJAYA S H S,GODAHEWA G I,JAYASOORIYA R G P T,et al.Antimicrobial effects of chitosan silver nano composites on fish pathogenic Aliivibrio(Vibrio)salmonicida[J].Aquaculture,2016,450:422-430.
[10]KIRUBA A,VENKATACHALAM R,VENKATACHALAM U,et al.Effect of Chitosan Supplemented Diet on Survival,Growth,Hematological,Biochemical and Immunological Responses of Indian Major Carp Labeo rohita[J].International Research Journal of Pharmacy.2013,4(5):141-147.
[11]LI T,NA R,YU P,et al.Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle[J].Czech Journal of Animal Science,2015,60(2):38-44.
[12]陈秀芸.硒化壳聚糖对蛋用公鸡生化指标硒酶活性及GPx4RNA表达水平的影响[D].乌鲁木齐:新疆农业大学,2013.
[13]王宜艳,孙虎山,刘玉坤.硒化壳聚糖对栉孔扇贝血细胞中部分免疫因子的影响[J].海洋通报,2007(2):117-120.
[14]孙虎山,李光友.硒化卡拉胶和酵母聚糖对栉孔扇贝血淋巴中两种水解酶活力的影响[J].海洋与湖沼,2002,33(3):245-249.
[15]索金良.4-硒(代)硫酸脂多糖的抗肿瘤免疫调节作用[J].生理科学进展,1996,27(1):43-46.
[16]方积年.多糖研究的现状[J].药学学报,1986,21(12):944-950.
[17]LYONS M P,PAPAZYAN T T,SURAI P F.Selenium in food chain and animal nutrition:Lessons from nature-Review[J].Asian-Australasian Journal of Animal Sciences,2007,20(7):1 135-1 155.
[18]ARTHINGTON J D.Effects of supplement type and selenium source on measures of growth and selenium status in yealing beef steers[J].Journal of Animal Science,2008,86:1 472-1 477.
[19]ROTRUCK J T,POPE A L,GANTHER H E,et al.Selenium:biochemical role as a component of glutathione peroxidase[J].Science,1973,79:588-592.
[20]卢美鸾,邱晓燕,郑森林,等.壳聚糖对肉鸡抗氧化能力及生产性能的作用[J].生态学杂志,2008,27(10):1 749-1 752.
[21]HILTON J W,HODSON P V,SLINGER S J.The requirement and toxicity of selenium in rainbow trout(Salmo gairderi)[J].The Journal of Nutrition,1980,110(12):2 527-2 535.
[22]GATLIN III D M,WILSON R P.Dietary selenium requirement of fingerling catfish[J].Journal of Nutrition,1984,114(3):627-633.
[23]WANG Weifang,MAI Kangsen,ZHANG Wenbing,et al.Dietary selenium requirement and its toxicity in juvenile abalone Haliotis discus hannai lno[J].Aquaculture,2012,330-333:42-46.
[24]LIN Yuhung,SHIAU Shiyen.Dietary selenium requirements of juvenile grouper,Epinephelus malabaricu s[J].Aquaculture,2005,250(1/2):356-363.
[25]PAMANTIDIS V,BERMANO G,VILLETTE S.Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon[J].FEBS Letters,2005,579(3):792-796.
[26]徐辉碧,杨祥良,刘琼.硒的生物疚的两面性[C]//中国营养学会.中国营养学会第七届微量元素营养学术会议论文摘要汇编,2001:16-25.
[27]张宇晴,卢桥发,甄海宁,等.谷胱甘肽、丙二醛联合检测对肺癌的诊断价值[J].临床内科杂志,2003,20(2):80-81.
[28]苏传福.草鱼幼鱼硒的营养需要研究[D].重庆:西南大学,2008.
[29]朱宏娟.不同硒源及硒水平对肉仔鸡生产性能和血液抗氧化指标的影响[D].长沙:湖南农业大学,2006.
[30]杨凡,屈亮,聂琴.酵母硒在水生动物上的应用研究[J].饲料研究,2013(10):36-38.
[31]曹丹,周洪亮.不同添加剂对暗纹东方魨生长和脾溶菌酶活力的影响[J].饲料广角,2002(11):18-19.
[32]常仁亮,韩保平,顾润润.硒酵母的培养及其养虾效果[J].水产学报,2000,24(5):458-462.
[33]王井亮,叶亮宏,周明.硒源和硒水平对克氏螯虾肉品质的影响[J].湖南饲料,2010(3):24-26.
[34]徐有卿,李太元,丁兆坤,等.添加酵母硒对鱤鱼消化酶活性鱼饲料转化率的影响[J].水产科学,2013,32(7):391-395.
[35]吕晨辉.富硒益生菌对高温条件下仔猪生产性能、抗氧化能力和肠道菌群的影响[D].南京:南京农业大学,2013.