硒化低聚氨基多糖稳定性研究
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摘要
以低聚氨基多糖与亚硒酸钠为原料,合成硒化低聚氨基多糖(Low molecular seleno-aminopolysaccharide,LSA)。通过无机硒与总硒含量的比值考察LSA稳定性,对LSA进行了影响因素[60℃高温试验,25℃、相对湿度(90%±5%)高湿度试验和(4500±500)lx强光照试验]、加速试验(40℃、相对湿度75%)和长期试验(25℃、相对湿度60%,12个月)。结果高湿度条件下10 d LSA吸湿严重发生结块现象,无机硒/总硒有显著性减小。高温及强光照射10 d对其质量也有一定影响,但无机硒/总硒在强光试验中有显著性增加,长时间的光照对LSA的稳定性影响较大。同时,加速试验与长期试验表明产品较为稳定。因此,硒化低聚氨基多糖有一定程度的吸湿性,高光对其稳定性的影响较大,应该在密闭条件下避光保存。
Sodium selenite and low molecular amino-polysaccharide were used to synthesize low molecular seleno-aminopolysaccharide(LSA) in this experiment by chemical method. The stability of LSA was studied by the ratio of organic to inorganic selenium. The appearance, the quality change and the ratio of organic to inorganic selenium were investigated by influential factor test [high temperature 60℃, high humidity test(25 ℃, relative humidity(90%±5%) and(4500±500)lx strong light test], accelerated test [40 ℃(relative humidity 75%)] and long-term test [25 ℃(relative humidity 60%) for 12 months]. The results showed that agglomeration was caused by the serious moisture under high humidity conditions in 10 d and the ratio of organic to inorganic selenium was decreased significantly. The quality was also change under high temperature and strong light test, and the ratio of organic to inorganic selenium was increased under strong light test. Results of accelerated test and long-term test showed that LSA was more stable. Conclusion, LSA has a certain degree of moisture absorption, while strong light had significant effect. It should be stored in light-resistant.
Sodium selenite and low molecular amino-polysaccharide were used to synthesize low molecular seleno-aminopolysaccharide(LSA) in this experiment by chemical method. The stability of LSA was studied by the ratio of organic to inorganic selenium. The appearance, the quality change and the ratio of organic to inorganic selenium were investigated by influential factor test [high temperature 60℃, high humidity test(25 ℃, relative humidity(90%±5%) and(4500±500)lx strong light test], accelerated test [40 ℃(relative humidity 75%)] and long-term test [25 ℃(relative humidity 60%) for 12 months]. The results showed that agglomeration was caused by the serious moisture under high humidity conditions in 10 d and the ratio of organic to inorganic selenium was decreased significantly. The quality was also change under high temperature and strong light test, and the ratio of organic to inorganic selenium was increased under strong light test. Results of accelerated test and long-term test showed that LSA was more stable. Conclusion, LSA has a certain degree of moisture absorption, while strong light had significant effect. It should be stored in light-resistant.
引文
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[19]王红连,张东升,张凌裳,等.硒化灵芝多糖制备及免疫学相关研究[J].食品科学,2009,30(17):316-318.
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[2]Rayman M P.Selenium and human health[J].Lancet,2012,379:1256-1268.
[3]Zhao B,Zhang J,Yao J,et al.Selenylation modification can enhance antioxidant activity of Potentilla anserina L.polysaccharide[J].International Journal of Biological Macromolecules,2013,58:320-328.
[4]XIAO Y,HUANG Q,ZHENG Z,et al.Construction of a Cordyceps sinensis exopolysaccharide-conjugated selenium nanoparticles and enhancement of their antioxidant activities[J].International Journal of Biological Macromolecules,2017,99:483-491.
[5]Schweizer U,Dehina N,Schomburg L.Disorders of selenium metabolism and selenoprotein function[J].Current Opinion in Pediatrics,2011,23:429.
[6]Todd S,Thomas D,Tucker L,et al.Selenium requirements in cats and dogs[M].2006.
[7]姚万玲.党参多糖的硒化修饰及其抗氧化和增强免疫活性的研究[D].南京:南京农业大学,2011.
[8]陈文霞.纹党多糖硒化物的制备、结构表征及其抗肿瘤活性研究[D].兰州:兰州大学,2015.
[9]秦慧玉.羧甲基壳聚糖缓控释凝胶新辅料的制备、表征及应用研究[D].武汉:武汉理工大学,2006.
[10]尹学琼,孙中亮,林强,等.壳聚糖基金属配合物材料及其应用现状[J].昆明:昆明理工大学学报(理工版),2002,27(3):78-93.
[11]郭振楚,吴同华.壳聚糖及其衍生物与金属配位的研究进展[C].甲壳素化学与应用研讨会,1999.
[12]王晓晖,白海泉,乌兰格日乐.多糖金属配合物的研究进展[J].内蒙古民族大学学报(自然科学版),2014,(5):516-519.
[13]郭向阳,王友明,高阳,等.硒化低聚氨基多糖制备工艺优化及其抗氧化活性研究[J].浙江海洋学院学报(自然科学版),2015,34(3):216-221.
[14]顾丽霞,郑斌,相兴伟,等.硒化低聚氨基多糖对RAW264.7细胞的免疫调节作用[J].食品科学,2018,39(13):198-204.
[15]Tapiero H,Townsend D M,Tew K D.The antioxidant role of selenium and seleno-compounds[J].Biomedicine&Pharmacotherapy,2003,57:134-144.
[16]Beckett G J,Aethur J R.Selenium and endocrine systems[J].Journal of Endocrinology,2005,184:455-65.
[17]Aathur J R,Mckenzie R C,Beckett G J.Selenium in the immune system[J].Journal of Nutrition,2003,133:1457S-1459S.
[18]刘宽辉,田卫军,高珍珍,等.硒化党参多糖和大蒜多糖协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效[J].畜牧兽医学报,2017,48(7):1349-1356.
[19]王红连,张东升,张凌裳,等.硒化灵芝多糖制备及免疫学相关研究[J].食品科学,2009,30(17):316-318.
[20]吕键.硒化壳聚糖和砷化壳聚糖的制备、结构及其性质分析[D].延边:延边大学,2004.