闲置厌氧氨氧化微生物的启动及微生物分析
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摘要
文章以闲置12个月的厌氧氨氧化生物膜填料重新启动厌氧氨氧化反应器,并对反应器的活性恢复情况、脱氮效果和微生物菌群结构开展研究。实验结果表明:在启动的200 d逐渐将进水氨氮、亚硝态氮浓度从50 mg/L提高到70 mg/L,水力停留时间从12 h缩短到4 h,后期氨氮去除率达80%以上,亚硝酸盐去除率达95%。170~200 d的稳定期中,平均去除负荷0.71±0.15 kg/(m~3·d)。另外,通过高通量测序技术对反应器中微生物群落变化情况进行了系统分析。启动过程中填料中污泥微生物浮霉菌门Planctomyctes的相对丰度从13.7%增长到了36.0%,成为优势菌群。
In this study,an anammox reactor was restarted up with used anammox biofilm stuffing which had lay idle for 12 months.Recovery of the biological activity,nitrogen removal and microbial community structure of the reactor were investigated.During 200 days of the restart-up period,concentrations of NH_4~+-N and NO_2~--N increased from 50 mg/L to 70 mg/L,and hydraulic retention time (HRT) was shortened from 12 h to 4 h gradually.The results indicated that the removal rate of NH_4~+-N-N and NO_2~--N in the later period reached to over 80% and 95% respectively.During the stable period between 170 and 200 d,the average nitrogen removal load was 0.71 ± 0.15 kg/(m~3 ·d).In addition,high-throughput sequencing technology was employed to analyze the change of microbial communities during the restart-up.Relative abundance of Planctomyctes species on the packing biofilms increased from 13.7% to 36.0% and therefore the species became the dominant.
In this study,an anammox reactor was restarted up with used anammox biofilm stuffing which had lay idle for 12 months.Recovery of the biological activity,nitrogen removal and microbial community structure of the reactor were investigated.During 200 days of the restart-up period,concentrations of NH_4~+-N and NO_2~--N increased from 50 mg/L to 70 mg/L,and hydraulic retention time (HRT) was shortened from 12 h to 4 h gradually.The results indicated that the removal rate of NH_4~+-N-N and NO_2~--N in the later period reached to over 80% and 95% respectively.During the stable period between 170 and 200 d,the average nitrogen removal load was 0.71 ± 0.15 kg/(m~3 ·d).In addition,high-throughput sequencing technology was employed to analyze the change of microbial communities during the restart-up.Relative abundance of Planctomyctes species on the packing biofilms increased from 13.7% to 36.0% and therefore the species became the dominant.
引文
[1]Strous M,Heijnen J J,Kuenen J G,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammoniumoxidizing microorganisms[J].Applied Microbiology&Biotechnology,1998,50(5):589-596.
[2]任宏洋,张代钧,丛丽影,等.厌氧氨氧化反应器启动及污泥产率系数测定[J].重庆大学学报(自然科学版),2008,31(1):88-92.
[3]Fux C,Siegrist H.Nitrogen removal from sludge digester liquids by nitrification/denitrification or partial nitritation/anammox:environmental and economical considerations[J].Water Science&Technology,2004,50(10):19-26.
[4]Abma W R,Schultz C E,Mulder J W,et al.Full-scale granular sludge Anammox process[J].Water Science&Technology,2007,55(8-9):27-33.
[5]Van der Star W R,Abma W R,Blommers D,et al.Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J].Water Research,2007,41(18):4149-4163.
[6]Ali M,Okabe S.Anammox-based technologies for nitrogen removal:advances in process start-up and remaining issues[J].Chemosphere,2015,141:144-153.
[7]Wang C C,Lee P H,Kumar M,et al.Simultaneous partial nitrification,anaerobic ammonium oxidation and denitrification(SNAD)in a fullscale landfill-leachate treatment plant[J].Journal of Hazardous Materials,2010,175(1-3):622-628.
[8]付丽霞,吴立波,宫玥,等.垃圾渗滤液中有机污染物对厌氧氨氧化的影响研究[J].水资源与水工程学报,2010,21(03):25-29.
[9]林琳,堵国成,陈坚.电子受体及中间产物对厌氧氨氧化的影响[J].应用与环境生物学报,2003,9(2):200-202.
[10]喻徐良,汪瑶琪,陈重军,等.ABR厌氧氨氧化反硝化协同脱氮除碳研究[J].工业安全与环保,2017,43(4):68-72.
[11]刘雪娇,田智勇,王思宇,等.容积负荷对ANAMMOX生物滤池脱氮效能的影响及其基质动力学[J].环境工程学报,2013,7(12):4619-4624.
[12]Zheng B,Zhang L,Guo J,et al.Suspended sludge and biofilm shaped different anammox communities in two pilot-scale one-stage anammox reactors[J].Bioresource Technology,2016,211:273-279.
[13]田智勇,李冬,杨宏,等.上向流厌氧氨氧化生物滤池的启动与脱氮性能[J].北京工业大学学报,2009,35(4):83-89.
[14]曹雁,王桐屿,秦玉洁,等.厌氧氨氧化反应器脱氮性能及细菌群落多样性分析[J].环境科学,2017,38(4):1544-1550.
[15]储昭瑞.厌氧氨氧化反应器启动及微生物群落结构解析[D].哈尔滨:哈尔滨工业大学,2012.
[16]Cao S,Du R,Li B,et al.High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating highstrength wastewater[J].Applied Microbiology&Biotechnology,2016,100(14):6457-6467.
[17]Kuenen J G.Anammox bacteria:from discovery to application[J].Nature Reviews Microbiology,2008,6(4):320-326.
[18]Quan Z X,Rhee S K,Zuo J E,et al.Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing(anammox)reactor[J].Environmental Microbiology,2008,10(11):3130-3139.
[19]Schmidt I,Sliekers O,Schmid M,et al.New concepts of microbial treatment processes for the nitrogen removal in wastewater[J].FEMSMicrobiology Reviews,2003,27(4):481-492.
[20]Puyol D,Carvajal-Arroyo J M,Garcia B,et al.Kinetic characterization of Brocadia spp.-dominated anammox cultures[J].Bioresource Technology,2013,139:94-100.
[21]Van der Star W R,Miclea A I,van Dongen U G J M,et al.The membrane bioreactor:a novel tool to grow anammox bacteria as free cells[J].Biotechnology&Bioengineering,2008,101(2):286-294.
[22]Fahrbach M,Kuever J,Meinke R,et al.Denitratisoma oestradiolicum gen.nov.,sp.nov.,a 17beta-oestradiol-degrading,denitrifying betaproteobacterium[J].International Journal of Systematic&Evolutionary Microbiology,2006,56(Pt 7):1547-1552.
[23]郑平,徐向阳,胡宝兰.新型生物脱氮理论与技术[M].北京:科学出版社,2004.
[24]任玉辉.低基质亚硝化与厌氧氨氧化脱氮效能及微生物特性研究[D].哈尔滨:哈尔滨工业大学,2014.
[25]Friedrich M,Takács I.A new interpretation of endogenous respiration profiles for the evaluation of the endogenous decay rate of heterotrophic biomass in activated sludge[J].Water Research,2013,47(15):5639-5646.
[2]任宏洋,张代钧,丛丽影,等.厌氧氨氧化反应器启动及污泥产率系数测定[J].重庆大学学报(自然科学版),2008,31(1):88-92.
[3]Fux C,Siegrist H.Nitrogen removal from sludge digester liquids by nitrification/denitrification or partial nitritation/anammox:environmental and economical considerations[J].Water Science&Technology,2004,50(10):19-26.
[4]Abma W R,Schultz C E,Mulder J W,et al.Full-scale granular sludge Anammox process[J].Water Science&Technology,2007,55(8-9):27-33.
[5]Van der Star W R,Abma W R,Blommers D,et al.Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J].Water Research,2007,41(18):4149-4163.
[6]Ali M,Okabe S.Anammox-based technologies for nitrogen removal:advances in process start-up and remaining issues[J].Chemosphere,2015,141:144-153.
[7]Wang C C,Lee P H,Kumar M,et al.Simultaneous partial nitrification,anaerobic ammonium oxidation and denitrification(SNAD)in a fullscale landfill-leachate treatment plant[J].Journal of Hazardous Materials,2010,175(1-3):622-628.
[8]付丽霞,吴立波,宫玥,等.垃圾渗滤液中有机污染物对厌氧氨氧化的影响研究[J].水资源与水工程学报,2010,21(03):25-29.
[9]林琳,堵国成,陈坚.电子受体及中间产物对厌氧氨氧化的影响[J].应用与环境生物学报,2003,9(2):200-202.
[10]喻徐良,汪瑶琪,陈重军,等.ABR厌氧氨氧化反硝化协同脱氮除碳研究[J].工业安全与环保,2017,43(4):68-72.
[11]刘雪娇,田智勇,王思宇,等.容积负荷对ANAMMOX生物滤池脱氮效能的影响及其基质动力学[J].环境工程学报,2013,7(12):4619-4624.
[12]Zheng B,Zhang L,Guo J,et al.Suspended sludge and biofilm shaped different anammox communities in two pilot-scale one-stage anammox reactors[J].Bioresource Technology,2016,211:273-279.
[13]田智勇,李冬,杨宏,等.上向流厌氧氨氧化生物滤池的启动与脱氮性能[J].北京工业大学学报,2009,35(4):83-89.
[14]曹雁,王桐屿,秦玉洁,等.厌氧氨氧化反应器脱氮性能及细菌群落多样性分析[J].环境科学,2017,38(4):1544-1550.
[15]储昭瑞.厌氧氨氧化反应器启动及微生物群落结构解析[D].哈尔滨:哈尔滨工业大学,2012.
[16]Cao S,Du R,Li B,et al.High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating highstrength wastewater[J].Applied Microbiology&Biotechnology,2016,100(14):6457-6467.
[17]Kuenen J G.Anammox bacteria:from discovery to application[J].Nature Reviews Microbiology,2008,6(4):320-326.
[18]Quan Z X,Rhee S K,Zuo J E,et al.Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing(anammox)reactor[J].Environmental Microbiology,2008,10(11):3130-3139.
[19]Schmidt I,Sliekers O,Schmid M,et al.New concepts of microbial treatment processes for the nitrogen removal in wastewater[J].FEMSMicrobiology Reviews,2003,27(4):481-492.
[20]Puyol D,Carvajal-Arroyo J M,Garcia B,et al.Kinetic characterization of Brocadia spp.-dominated anammox cultures[J].Bioresource Technology,2013,139:94-100.
[21]Van der Star W R,Miclea A I,van Dongen U G J M,et al.The membrane bioreactor:a novel tool to grow anammox bacteria as free cells[J].Biotechnology&Bioengineering,2008,101(2):286-294.
[22]Fahrbach M,Kuever J,Meinke R,et al.Denitratisoma oestradiolicum gen.nov.,sp.nov.,a 17beta-oestradiol-degrading,denitrifying betaproteobacterium[J].International Journal of Systematic&Evolutionary Microbiology,2006,56(Pt 7):1547-1552.
[23]郑平,徐向阳,胡宝兰.新型生物脱氮理论与技术[M].北京:科学出版社,2004.
[24]任玉辉.低基质亚硝化与厌氧氨氧化脱氮效能及微生物特性研究[D].哈尔滨:哈尔滨工业大学,2014.
[25]Friedrich M,Takács I.A new interpretation of endogenous respiration profiles for the evaluation of the endogenous decay rate of heterotrophic biomass in activated sludge[J].Water Research,2013,47(15):5639-5646.