鼻渊合剂对急性鼻窦炎模型大鼠鼻窦黏膜p38MAPK信号通路及IL-9/IL-10的影响
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摘要
目的观察鼻渊合剂治疗急性鼻窦炎的临床疗效,并探讨其对大鼠急性鼻窦炎模型鼻窦黏膜p38MAPK信号通路及IL?9、IL-10的影响。方法建造大鼠急性鼻窦炎模型,设中、西药组、生理盐水组,分别使用鼻渊合剂、克拉霉素分散片及桉柠蒎肠溶软胶囊、生理盐水灌胃,观察症状积分,并设空白组,测定造模后0、7、14、21d鼻窦黏膜IL-9、IL-10及p-p38水平,进行统计学分析。结果通过大鼠症状积分比较,表明中、西药都有治疗作用。造模后大鼠IL-9及p-p38水平明显增高;用药后中药、西药组大鼠鼻窦黏膜IL-9及p-p38水平随治疗周期明显下降,与生理盐水组比较有显著性差异(P<0.05),中药组IL-9及p-p38水平下降与西药组无显著性差异。用药后中药组、西药组及生理盐水组鼻窦黏膜IL-10水平随治疗周期逐步升高,中药组、西药组与生理盐水组比较均存在显著性差异(P<0.05),中药组与西药组比较无显著性差异。结论鼻渊合剂治疗急性鼻窦炎有确切疗效,IL-9、IL-10参与了急性鼻窦炎发病的免疫过程,与p38MAPK通路存在一定关系,鼻渊合剂有促进IL-10、抑制p-p38活化阻断p38MAPK通路抑制IL-9产生的作用。
OBJECTIVE To explore the clinical effect of Acute rhinosinusitis(ARS)treated by of Biyuan Mixture and its effects on p38 MAPK signaling pathway and IL-9 and IL-10 of the sinus mucosa of ARS in modeled rats.METHODS To summarize the clinical curative effects of the modeled rats,20 cases of rats in each group,which was the Chinese medicine group,western medicine group and saline group.Those rats were respectively filled the stomach with Biyuan mixture,clarithromycin dispersibl tablets and limonene and pinene enteric soft capsules,saline.Meanwhile,5 rats were selected as the blank group.The sinus mucosa IL-9,IL-10 and p-p38 were measured before treatment,and after treatment(0,7,14,21 d).And the data were recorded for statistical analysis.RESULTS According to the symptom score comparison,both Chinese medicine and western medicine have the therapeutic effects.Compared with the blank group,the levels of IL-9 and p-p38 were significantly increased.The levels of IL-9 and p-p38 in the sinus mucosa of the Chinese medicine group and the western medicine group decreased significantly with the treatment cycle(P<0.05),and there was no significant difference between the two groups.The level of IL-10 in the sinus mucosa of the Chinese medicine group,the western medicine group and the saline group gradually increased with the treatment cycle,and there were differences between the Chinese medicine group,the western medicine group and the saline group(P<0.05),while there was no significant difference between the Chinese medicine group and the western medicine group.CONCLUSION IL-9 and IL-10 are involved in the immune process of the onset of ARS,and there is a certain relationship with p38 MAPK pathway.ARS treated by Biyuan Mixture has a definite therapeutic effect and causes changes in the level of the above substances,indicating that this prescription can promote IL-10,inhibit p-p38 activation and may block p38 MAPK pathway to inhibit IL-9.
OBJECTIVE To explore the clinical effect of Acute rhinosinusitis(ARS)treated by of Biyuan Mixture and its effects on p38 MAPK signaling pathway and IL-9 and IL-10 of the sinus mucosa of ARS in modeled rats.METHODS To summarize the clinical curative effects of the modeled rats,20 cases of rats in each group,which was the Chinese medicine group,western medicine group and saline group.Those rats were respectively filled the stomach with Biyuan mixture,clarithromycin dispersibl tablets and limonene and pinene enteric soft capsules,saline.Meanwhile,5 rats were selected as the blank group.The sinus mucosa IL-9,IL-10 and p-p38 were measured before treatment,and after treatment(0,7,14,21 d).And the data were recorded for statistical analysis.RESULTS According to the symptom score comparison,both Chinese medicine and western medicine have the therapeutic effects.Compared with the blank group,the levels of IL-9 and p-p38 were significantly increased.The levels of IL-9 and p-p38 in the sinus mucosa of the Chinese medicine group and the western medicine group decreased significantly with the treatment cycle(P<0.05),and there was no significant difference between the two groups.The level of IL-10 in the sinus mucosa of the Chinese medicine group,the western medicine group and the saline group gradually increased with the treatment cycle,and there were differences between the Chinese medicine group,the western medicine group and the saline group(P<0.05),while there was no significant difference between the Chinese medicine group and the western medicine group.CONCLUSION IL-9 and IL-10 are involved in the immune process of the onset of ARS,and there is a certain relationship with p38 MAPK pathway.ARS treated by Biyuan Mixture has a definite therapeutic effect and causes changes in the level of the above substances,indicating that this prescription can promote IL-10,inhibit p-p38 activation and may block p38 MAPK pathway to inhibit IL-9.
引文
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[6]陈婧,方建国,吴方建,等.鱼腥草抗炎药理作用机制的研究进展[J].中草药,2014,45(2):284-289.
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[9]KUMAR S,BOEHM J,LEE JC.p38MAP kinases:key signalling molecules as thrapeutic targets for inflammatory disease[J].Nat Rev Drug Discov,2003,2:717-726.
[2]吴拥军,马华安,黄洁,等.鼻渊合剂对功能性鼻窦内窥镜术后术腔黏膜上皮化影响的临床观察[J].辽宁中医杂志,2010,37(12):2369-2370.
[3]侯辉,李浩.辛夷对家兔气管纤毛传输速度影响的初步观察[J].中国中西医结合耳鼻咽喉科杂志,1998,6(3):141-142.
[4]郑辉,王德才.白芷的药理作用与临床应用研究进展[J].医药导报,2009,28(1):83-86.
[5]顾静荣,冯莉莉,罗建伟,等.鱼腥草的药理作用及临床应用新进展[J].海峡药学,2006,18(4):121-123.
[6]陈婧,方建国,吴方建,等.鱼腥草抗炎药理作用机制的研究进展[J].中草药,2014,45(2):284-289.
[7]KYRIAKIS JM,AVRUCH J.Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation[J].Physiol Rev,2001,81:807-869.
[8]SCHINDLER JF,MONAHAN JB,SMITH WG.P38pathway kinases as anti-inflammatory drug targets[J].Dent Res,2007,86:800-811.
[9]KUMAR S,BOEHM J,LEE JC.p38MAP kinases:key signalling molecules as thrapeutic targets for inflammatory disease[J].Nat Rev Drug Discov,2003,2:717-726.