不同种质地黄块根菊花心表型性状及HPLC指纹图谱特征分析
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摘要
目的:探讨不同种质地黄块根菊花心表型性状及高效液相(HPLC)指纹图谱特征。方法:观察测量地黄整个生育期内菊花心形状及比例直径的变化,同时建立表征地黄块根、菊花心及非菊花心部位化学质量的HPLC指纹图谱,并采用主成分和系统性聚类方法对其进行分析。结果:不同种质地黄的菊花心形状及大小存在明显差异且具有一定的规律性;指纹图谱显示不同种质地黄不同部位的化学成分种类具有一致性,其化学质量特征的差异主要在于化学成分含量及其相对比例的不同;主成分分析结果表明毛蕊花糖苷、梓醇、益母草苷、地黄苷A和地黄苷D等有效成分基本均参与了各种质地黄不同部位的质量表达,但每种成分在各种质地黄不同部位之间质量差异表达中的贡献率不同,同时参与这种差异表达的其他成分在各种质地黄中的贡献率也不同;系统性聚类分析表明,北京1号,沁怀,1706及沁怀郑地黄的菊花心与非菊花心化学质量特征差异较大,而85-5,白选地黄的菊花心与非菊花心部位化学质量特征差异较小。结论:不同种质地黄菊花心表型性状及其HPLC指纹图谱特征具有一定的差异。
Objective: To discuss the phenotypic character and the HPLC fingerprints of radial striations from different germplasms Rehmanniae Radix. Method: The changes in the shape and column diameter of the radial striations of Rehmanniae Radix were observed and measured in the whole growth period. Besides,the HPLC fingerprints of the root,radial and un-radial striations were established to sign the chemical quality and analyzed by principal component analysis( PCA) and systematic cluster analysis. Result: There were significantly differences and regularities in the shape and proportion of the radial striations of different germplasms Rehmanniae Radix. The fingerprints showed the consistency between different types of chemical ingredients,and the differences in chemical quality characteristics mainly lay in the content of chemical compositions and theirs relative ratio. The results of PCA indicated that active ingredients,such as acteoside, catalpol, rehmaionoside D, rehmaionoside A and leonuride,were involved in the quality expression of different parts from various germplasms of Rehmanniae Radix,but each ingredient had a distinctive contribution rate to the differential quality expression between different parts from various germplasms of Rehmanniae Radix. However,the other components involved in the differential quality expression had different contribution rates in different germplasms. The systematic cluster analysis indicated that great differences in the chemical quality between the radial striations and un-radial striations of Beijing-1,Qinhuai,Qinhuai Zheng and 1706 germplasms,but with small differences in 85-5 and Baixuan germplasms. Conclusion:There are differences in phenotypic character of the radial striations and HPLC fingerprints between different germplasms Rehmanniae Radix.
Objective: To discuss the phenotypic character and the HPLC fingerprints of radial striations from different germplasms Rehmanniae Radix. Method: The changes in the shape and column diameter of the radial striations of Rehmanniae Radix were observed and measured in the whole growth period. Besides,the HPLC fingerprints of the root,radial and un-radial striations were established to sign the chemical quality and analyzed by principal component analysis( PCA) and systematic cluster analysis. Result: There were significantly differences and regularities in the shape and proportion of the radial striations of different germplasms Rehmanniae Radix. The fingerprints showed the consistency between different types of chemical ingredients,and the differences in chemical quality characteristics mainly lay in the content of chemical compositions and theirs relative ratio. The results of PCA indicated that active ingredients,such as acteoside, catalpol, rehmaionoside D, rehmaionoside A and leonuride,were involved in the quality expression of different parts from various germplasms of Rehmanniae Radix,but each ingredient had a distinctive contribution rate to the differential quality expression between different parts from various germplasms of Rehmanniae Radix. However,the other components involved in the differential quality expression had different contribution rates in different germplasms. The systematic cluster analysis indicated that great differences in the chemical quality between the radial striations and un-radial striations of Beijing-1,Qinhuai,Qinhuai Zheng and 1706 germplasms,but with small differences in 85-5 and Baixuan germplasms. Conclusion:There are differences in phenotypic character of the radial striations and HPLC fingerprints between different germplasms Rehmanniae Radix.
引文
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[2]谢宗万.地黄的本草学研究[J].中医杂志,1990,31(5):48-50.
[3]翟胜利.“道地药材”是保证中药疗效的根源[J].首都医药,2006,8(15):34-37.
[4]张金鼎,曹鸿云.河南四大怀药[J].中药材,1987,29(3):55-56.
[5]王太霞,李建军,李景原,等.不同产区地黄的比较研究[J].河南农业科学,2005,23(10):69-73.
[6]董诚明,乔毅琳,曹利华.不同品种怀地黄断面与其质量相关性分析研究[C]//2013全国中药与天然药物高峰论坛暨第十三届全国中药和天然药物学术研讨会论文集,2013-06-16,中国浙江杭州:出版社不详,2013:57.
[7]刘孟奇,王小巧,陈随清,等.地黄的组织化学研究[J].中药材,2013,36(11):1771-1773.
[8]谢彩侠,张苗,李雅静,等.怀地黄块根中菊花心与非菊花心部位的化学质量特征比较[J].中国中药杂志,2017,42(21):4172-4179.
[9]谢彩侠,李雅静,张苗,等.不同品种地黄块根菊花心与非菊花心部位有效成分特征分析[J].中国实验方剂学杂志,2018,24(20):75-83.
[10]王丰青,王丽娜,智惊宇,等.不同品种地黄中毛蕊花糖苷的动态积累规律变化[J].中国实验方剂学杂志,2017,23(24):78-83.
[11]王丰青,田云鹤,黄勇,等. 18个地黄种质的表型性状及相关统计学分析[J].植物资源与环境学报,2015,24(1):28-35.
[12]黄永亮,余志杰,黎江华,等.熟三七饮片HPLC指纹图谱的建立及多成分定量测定[J].中草药,2018,49(3):589-595.
[13]孟菲,桂新景,秦会珍,等.中药复方制剂益艾康胶囊HPLC指纹图谱研究[J].中国新药杂志,2018,27(7):839-842.
[14]杨彦坤,独家能,姚静,等.基于HPLC及GC-MS技术的血尿胶囊融合指纹图谱[J].药物分析杂志,2018,38(1):158-167.