原子吸收光谱法测定桦褐孔菌中7种金属元素的总质量比及形态分析
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摘要
利用火焰原子吸收光谱法测定桦褐孔菌中的Mn,Ca,Zn,Fe元素,石墨炉原子吸收光谱法测定桦褐孔菌中的Pb,Cu,Cr元素.用微波消解法获得桦褐孔菌的金属元素总质量比,超声水提取法获得水溶态金属元素的初级形态,最后用Tessier逐级提取法对获取的5种形态金属元素进行次级形态分析.结果表明:7种金属元素回归方程的线性良好,相关系数大于0.99,检出限为0.007～2.989ng/mL;桦褐孔菌中Cu主要以碳酸盐结合态和残渣态的形式存在,Ca,Mn,Zn,Fe主要以离子交换态和残渣态的形式存在,Cr主要以残渣态的形式存在,Pb主要以碳酸盐结合态的形式存在.
Mn,Ca,Zn,Fe in Inonotus obliquus were detected by flame atomic absorption spectrometry,and Pb,Cu,Cr were detected by graphite furnace atomic absorption spectroscopy.Microwave digestion was used to obtain total mass ratio of metal elements in I.obliquus.The primary forms of water soluble metal elements were gained by ultrasonic water extraction method,and the secondary speciation analysis of five metal elements were carried out by Tessier step by step extraction method.The results show that the linearity of regression equation of seven metal elements is good,the correlation coefficients are greater than 0.99,and the detection limits is 0.007—2.989 ng/mL.Cu in Io.bliquus mainly exists in the form of carbonate-bound and residue.Ca,Mn,Zn and Fe elements mainly exist in the form of ion exchange and residue,Cr mainly exists in the form of residue,and Pb mainly exists in the form of carbonate-bound.
Mn,Ca,Zn,Fe in Inonotus obliquus were detected by flame atomic absorption spectrometry,and Pb,Cu,Cr were detected by graphite furnace atomic absorption spectroscopy.Microwave digestion was used to obtain total mass ratio of metal elements in I.obliquus.The primary forms of water soluble metal elements were gained by ultrasonic water extraction method,and the secondary speciation analysis of five metal elements were carried out by Tessier step by step extraction method.The results show that the linearity of regression equation of seven metal elements is good,the correlation coefficients are greater than 0.99,and the detection limits is 0.007—2.989 ng/mL.Cu in Io.bliquus mainly exists in the form of carbonate-bound and residue.Ca,Mn,Zn and Fe elements mainly exist in the form of ion exchange and residue,Cr mainly exists in the form of residue,and Pb mainly exists in the form of carbonate-bound.
引文
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[3]于婧路,冯晓玲.桦褐孔菌抗肿瘤机制的研究进展[J].实用肿瘤学杂志,2017,31(1):69-72.(YU Jinglu,FENG Xiaoling.The Research Progress in Antitumor Mechansim of Inonotus obliquus[J].Practical Oncology Journal,2017,31(1):69-72.)
[4]XU Xiangqun,WU Yongde,CHEN Hui.Comparative Antioxidative Characteristics of Polysaccharide Enriched Extracts from Natural Sclerotia and Cultured Mycelia in Submerged Fermcntation of Inonotus obliquus[J].Food Chemistry,2011,127(1):74-79.
[5]KIM Y O,HAN S B,LEE H W,et al.Immuno-Stimulating Effect of the Endo-polysaccharide Produced by Submerged Culture of Inonotus obliquus[J].Life Sciences,2005,77(19):2438-2456.
[6]崔杰,于新,王立勋,等.桦褐孔菌化学成分和药理作用研究进展[J].河北中医药学报,2017,32(3):48-53.(CUI Jie,YU Xin,WANG Lixun,et al.Advances in Studies on Chemical Constituents and Pharmacological Activities of Inoborus Obliquus[J].Journal of Hebei TCM and Pharmacology,2017,32(3):48-53.)
[7]DIAO Baozhong,JIN Weirong,YU Xuejing.Protective Effect of Polysaccharides from Inonotus obliquus on Streptozotocin-Induced Diabetic Symptoms and Their Potential Mechanisms in Rats[J].Evidence-Based Complementary and Alternative Medicine,2014,2014:841496-1-841496-5.
[8]PODLASIN'SKA J,PROSKURAN N,SZYMAN'SKA A.Content of Pb,Hg,Zn,Mn,Cu and Fe in Macrofungi Collected from Wkrzanska Forest in Northwestern Poland[J].Polish Journal of Environmental Studies,2015,24(2):651-656.
[9]HWANG B S,LEE I K,YUN B S.Phenolic Compounds from the Fungus Inonotus obliquus and Their Antioxidant Properties[J].The Journal of Antibiotics,2016,69(2):108-110.
[10]包玉敏,张力,徐玲,等.蒙药材金莲花中5种微量元素的形态分析[J].吉林大学学报(理学版),2011,49(2):327-330.(BAO Yumin,ZHANG Li,XU Ling,et al.Species Analysis of Five Trace Elements in Trollius chinensis[J].Journal of Jilin University(Science Edition),2011,49(2):327-330.)
[11]TESSIER A,CAMPBELL P G C,BISSON M.Sequential Extraction Procedure for the Speciation of Particulate Trace Metals[J].Analytical Chemistry,1979,51(7):844-851.