川木瓜多糖的化学修饰及其活性研究
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摘要
目的研究化学修饰对川木瓜多糖抗氧化活性和α-葡萄糖苷酶抑制作用的影响。方法对川木瓜多糖进行羧甲基化、乙酰化、硫酸化修饰,用红外光谱表征结构,采用体外试验法测定川木瓜多糖及化学修饰产物的抗氧化活性及对α-葡萄糖苷酶的抑制作用。结果红外光谱显示,化学修饰多糖除了有原多糖的红外吸收外,还有羧甲基、乙酰基、硫酸基的特征吸收峰。抗氧化活性试验表明各组分对OH·和DPPH·具有一定清除作用,硫酸化多糖清除能力最强。羧甲基川木瓜多糖对α-葡萄糖苷酶的抑制作用较好,强于修饰前的活性。结论通过化学修饰可增强川木瓜多糖的抗氧化活性和对α-葡萄糖苷酶的抑制作用。
Objective To investigate the effect of chemical modification on antioxidant activity of Chaenomeles speciosa polysaccharide and its inhibitory effect onα-glucosidase. Methods The Chaenomeles speciosa polysaccharide was modified by carboxymethylation,acetylation and sulfur acidification.The structure was characterized by infrared spectrum.The antioxidant activity of Chaenomeles speciosa polysaccharide and its chemical modified products and its inhibitory effect onα-glucosidase were determined in vitro. Results The infrared spectrum showed that the chemically modified polysaccharides not only had the infrared absorption of the original polysaccharides,but also had the characteristic absorption peaks of carboxymethyl,acetyl and sulfuric acid groups.The antioxidant activity test showed that each component had a certain scavenging effect on OH·and DPPH· and the removal ability of sulfide polysaccharides was the strongest.The carboxymethyl Chaenomeles speciosa polysaccharide had a good inhibitory effect onα-glucosidase,which was stronger than that before modification. Conclusion The antioxidant activity of Chaenomeles speciosa polysaccharide and its inhibitory effect onα-glucosidase can be enhanced by chemical modification.
Objective To investigate the effect of chemical modification on antioxidant activity of Chaenomeles speciosa polysaccharide and its inhibitory effect onα-glucosidase. Methods The Chaenomeles speciosa polysaccharide was modified by carboxymethylation,acetylation and sulfur acidification.The structure was characterized by infrared spectrum.The antioxidant activity of Chaenomeles speciosa polysaccharide and its chemical modified products and its inhibitory effect onα-glucosidase were determined in vitro. Results The infrared spectrum showed that the chemically modified polysaccharides not only had the infrared absorption of the original polysaccharides,but also had the characteristic absorption peaks of carboxymethyl,acetyl and sulfuric acid groups.The antioxidant activity test showed that each component had a certain scavenging effect on OH·and DPPH· and the removal ability of sulfide polysaccharides was the strongest.The carboxymethyl Chaenomeles speciosa polysaccharide had a good inhibitory effect onα-glucosidase,which was stronger than that before modification. Conclusion The antioxidant activity of Chaenomeles speciosa polysaccharide and its inhibitory effect onα-glucosidase can be enhanced by chemical modification.
引文
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[2]王有为,何敬胜,范建伟,等.木瓜道地起源与道地产区形成研究:2009年全国中药学术研讨会论文集[C].2009.
[3]曹莉莉,李亮,王芳,等.羧甲基化修饰余甘多糖及生物活性研究[J].中国食品学报,2017,17(10):57-63.
[4]赵鹏,张婷婷,宋逍.倒卵叶五加多糖的乙酰化工艺研究[J].天然产物研究与开发,2014,26(9):1492-1496.
[5]杨洋,王一峰,马诣欣,等.楤木根皮多糖硫酸酯的制备、结构表征及抗氧化活性的研究[J].广西植物,2018,38(7):911-923.
[6]李容,梁榕珊,覃涛,等.白茅根多糖抗氧化活性及抑制α-葡萄糖苷酶活性研究[J].食品研究与开发,2014,35(7):9-12.
[7]赵永安,陈冠,陶遵威.苦豆子多糖及其衍生物的体外抗氧化活性[J].中国实验方剂学杂志,2013,19(5):229-232.
[8]刘丹丹,韩唯凡,蒋建新,等.无患子果皮多糖微波提取工艺及抗氧化活性研究[J].林产化学与工业,2018,38(4):109-116.
[9]邱明阳,赵武,刘玉婷,等.五味子抗氧化及抑制α-葡萄糖苷酶活性的比较研究[J].天然产物研究与开发,2018,30(5):840-846.
[10]杨夏,孙从永,余江南,等.玉米须酸性多糖的结构表征及降血脂活性[J].江苏大学学报(医学版),2018,28(4):333-338.
[11] Wu Y,Ye M,Du Z,et al.Carboxymethylation of an exopolysaccharide from Lachnum and effect of its derivatives on experimental chronic renal failure[J].Carbohydrate Polymers,2014,114:190-195.
[12]谢建华.青钱柳多糖的分子修饰及生物活性研究[D].南昌:南昌大学,2014.
[13]赵芷芊,王敏,张志清.植物多糖的提取及抗氧化功效的研究进展[J].食品工业科技,2018,39(13):337-342.
[14]谭西,周欣,陈华国.植物多糖构效关系研究进展[J].中国中药杂志,2017,42(21):4104-4109.