罂粟体内蒂巴因变化规律及水氮耦合对蒂巴因的影响
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摘要
该文采用HPLC法,分析测定一年生罂粟植株体开花后不同生育期根、茎、叶、果壳中的蒂巴因含量,研究了罂粟体内蒂巴因的变化规律和水氮耦合对蒂巴因的影响。结果表明:一年生罂粟植株体的蒂巴因含量的变化范围在果壳中为1.81%～4.54%,成熟期达到最高;叶片中为0.30%～0.68%,膨大后期最高,采收期最低;根中为0.03%～0.28%,膨大前期最高,采收期最低;茎秆中为0.23%～0.60%,呈递减趋势,采收期最低,茎上、中、下部的蒂巴因含量的变化范围分别为0.42%～0.97%、0.15%～0.60%、0.13%～0.37%。滴灌量和施氮量对一年生罂粟果壳中蒂巴因含量的影响差异显著。罂粟壳质量最佳的水氮偶合条件为I_(130)N_(14),即滴灌量为130 m~3·667 m~(-2),施氮量为14 kg·667 m~(-2)时,蒂巴因含量达最高。
The HPLC was used to determine the contents of thebaine in roots,stems,leaves and shells of different growth stages after annual poppy flowering,to study the changes of thebaine in poppy and water-nitrogen coupling patterns effects under drip irrigation. The results showed that the content of thebaine in annual poppy shell was 1.81%-4.54%,and the highest in maturity. The leaf was 0.30%-0.68%,the highest in the late period of expansion and the lowest in the harvesting period. The root was 0.03%-0.28%,the highest in the early period of expansion and the lowest in the harvesting period. The stem was 0. 23%-0. 60%,showing a decreasing trend and the lowest in harvesting period. The content of thebaine in the upper,middle and lower parts of the stem was 0.42%-0.97%,0.15%-0.60% and0.13%-0.37%,respectively. The quantity of drip irrigation and nitrogen fertilization significantly effected the thebaine content in annual poppy. The best water-nitrogen coupling conditions for thebaine content in annual poppy shells was I_(130)N_(14). The thebaine content was the highest when the nitrogen application were 14 kg· 667 m~(-2) and the drip irrigation amount was 130 m~3· 667 m~(-2).
The HPLC was used to determine the contents of thebaine in roots,stems,leaves and shells of different growth stages after annual poppy flowering,to study the changes of thebaine in poppy and water-nitrogen coupling patterns effects under drip irrigation. The results showed that the content of thebaine in annual poppy shell was 1.81%-4.54%,and the highest in maturity. The leaf was 0.30%-0.68%,the highest in the late period of expansion and the lowest in the harvesting period. The root was 0.03%-0.28%,the highest in the early period of expansion and the lowest in the harvesting period. The stem was 0. 23%-0. 60%,showing a decreasing trend and the lowest in harvesting period. The content of thebaine in the upper,middle and lower parts of the stem was 0.42%-0.97%,0.15%-0.60% and0.13%-0.37%,respectively. The quantity of drip irrigation and nitrogen fertilization significantly effected the thebaine content in annual poppy. The best water-nitrogen coupling conditions for thebaine content in annual poppy shells was I_(130)N_(14). The thebaine content was the highest when the nitrogen application were 14 kg· 667 m~(-2) and the drip irrigation amount was 130 m~3· 667 m~(-2).
引文
CHANG Y,CHENG NN,LI YR,et al.,2010.Effects of salt stress on seed germination of two wild poppies[J].Till Cult,(2):3-5.[常瑛,陈凌娜,李彦荣,等,2010.盐胁迫对两种野罂粟种子萌发的影响[J].耕作与栽培,(2):3-5.]
HUO XM,1999.Determination of thebaine in various tissues of Papaver bracteatum by gas chromatography[J].Chin J Chromatogr,17(1):70-72.[霍秀敏,1999.红花罂粟植物的各个部分中蒂巴因含量的测定[J].色谱,17(1):70-72.]
LI YR,CHANG Y,WEI YJ,et al.,2012.Effects of temperature stress on germination and vitality of Papaver somniferum seeds[J].Guihaia,32(5):674-678.[李彦荣,常瑛,魏玉杰,等,2012.温度胁迫对罂粟种子萌发影响及其活力变化规律的研究[J].广西植物,32(5):674-678.]
LUO SZ,YU HX,ZHAO JR,et al.,Determination of morphine content in different parts of poppy plant[J].Trop Agric Eng,36(6):4-7.[雒淑珍,于红霞,赵继荣,等,2012.罂粟植株不同部位吗啡含量测试[J].热带农业工程,36(6):4-7.]
National Pharmacopoeia Committee,2000.Pharmacopoeia of People's Republic of China[M].Beijing:Chemical Industry Press:302-303.[国家药典委员会,2000.中华人民共和国药典[M].北京:化学工业出版社:302-303.]
SONG XL,LIU Q,2005.Determination of thebaine in red poppy by high performance liquid chromatography[J].Anal test Technol Instr,11(2):146-148.[宋小兰,刘强,2005.HPLC法测定红花罂粟中蒂巴因的含量[J].分析测试技术与仪器,11(2):146-148.]
WANG JQ,HE QX,LEI YH,et al.,2010.The changes of morphine content in opium poppy under different irrigation[J].Chin Agric Sci Bull,26(9):156-161.[王军强,何庆祥,雷耀湖,等,2010.不同灌水模式下罂粟吗啡含量的变化[J].中国农学通报,26(9):156-161.]
WEI YJ,ZHANG MX,CHANG Y,et al.,2009.A study on pollen germination of red poppy[J].J Shihezi Univ(Nat Sci Ed),27(4):433-436.[魏玉杰,张梅秀,常瑛,等,2009.红花罂粟花粉萌发的研究[J].石河子大学学报(自然科学版),27(4):433-436.]
XING JJ,ZHOU XB,LI J,2015.Research progress on treatment of opioid dependence with Buprenloxanone[J].WChin J Pharm Sci,(6):732-735.[邢静静,周晓波,李静,2015.丁丙诺啡纳洛酮治疗阿片类物质依赖患者的研究进展[J].华西药学杂志,(6):732-735.]
HUO XM,1999.Determination of thebaine in various tissues of Papaver bracteatum by gas chromatography[J].Chin J Chromatogr,17(1):70-72.[霍秀敏,1999.红花罂粟植物的各个部分中蒂巴因含量的测定[J].色谱,17(1):70-72.]
LI YR,CHANG Y,WEI YJ,et al.,2012.Effects of temperature stress on germination and vitality of Papaver somniferum seeds[J].Guihaia,32(5):674-678.[李彦荣,常瑛,魏玉杰,等,2012.温度胁迫对罂粟种子萌发影响及其活力变化规律的研究[J].广西植物,32(5):674-678.]
LUO SZ,YU HX,ZHAO JR,et al.,Determination of morphine content in different parts of poppy plant[J].Trop Agric Eng,36(6):4-7.[雒淑珍,于红霞,赵继荣,等,2012.罂粟植株不同部位吗啡含量测试[J].热带农业工程,36(6):4-7.]
National Pharmacopoeia Committee,2000.Pharmacopoeia of People's Republic of China[M].Beijing:Chemical Industry Press:302-303.[国家药典委员会,2000.中华人民共和国药典[M].北京:化学工业出版社:302-303.]
SONG XL,LIU Q,2005.Determination of thebaine in red poppy by high performance liquid chromatography[J].Anal test Technol Instr,11(2):146-148.[宋小兰,刘强,2005.HPLC法测定红花罂粟中蒂巴因的含量[J].分析测试技术与仪器,11(2):146-148.]
WANG JQ,HE QX,LEI YH,et al.,2010.The changes of morphine content in opium poppy under different irrigation[J].Chin Agric Sci Bull,26(9):156-161.[王军强,何庆祥,雷耀湖,等,2010.不同灌水模式下罂粟吗啡含量的变化[J].中国农学通报,26(9):156-161.]
WEI YJ,ZHANG MX,CHANG Y,et al.,2009.A study on pollen germination of red poppy[J].J Shihezi Univ(Nat Sci Ed),27(4):433-436.[魏玉杰,张梅秀,常瑛,等,2009.红花罂粟花粉萌发的研究[J].石河子大学学报(自然科学版),27(4):433-436.]
XING JJ,ZHOU XB,LI J,2015.Research progress on treatment of opioid dependence with Buprenloxanone[J].WChin J Pharm Sci,(6):732-735.[邢静静,周晓波,李静,2015.丁丙诺啡纳洛酮治疗阿片类物质依赖患者的研究进展[J].华西药学杂志,(6):732-735.]