银屑病患者皮损间充质干细胞磷脂酶C-β4和视黄醇脱氢酶10表达水平的研究
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摘要
目的:通过研究银屑病患者皮损处真皮间充质干细胞(DMSCs)中磷脂酶C-β4(PLCB4)和视黄醇脱氢酶(RDH)10mRNA的表达水平,进一步探讨银屑病发病的细胞及分子机制。方法:对24例寻常性银屑病(PV)患者和22例正常人皮肤DMSCs进行分离培养,流式细胞术进行细胞表型鉴定,实时荧光定量聚合酶链式反应(PCR)测定PLCB4和RDH10 mRNA表达水平。结果:PV组与正常对照组DMSCs形态无差异。PV组患者DMSCs中PLCB4 m RNA的表达水平是正常对照组的3.35倍,RDH10 mRNA的表达水平是正常对照组的1.17倍。结论:银屑病患者DMSCs中PLCB4和RDH10 m RNA表达均增高,可能影响相关信号通路,进而对银屑病发病产生影响。
Objective: To assess expression levels of phospholipase C-beta 4(PLCB4) and retinol dehydrogenase 10(RDH10)mRNA in dermal mesenchymal stem cells(DMSCs) from lesional skin of psoriatic patients in order to elucidate the cellular and molecular pathomechanisms of psoriasis. Methods: DMSCs from 24 patients with psoriasis vulgaris and 22 normal controls were cultured. Cell phenotypes were identified by flow cytometry, while expression levels of PLCB4 and RDH10 mRNA were determined using real-time fluorescence quantitative PCR. Results: While the morphology of DMSCs was similar between the two groups, expression levels of PLCB4 mRNA in psoriatic DMSCs were 3.35 folds of that in normal controls. Expression levels of RDH10 mRNA in psoriatic DMSCs were 1.17 folds of that in normal controls. Conclusion: The increased expression levels of PLCB4 and RDH10 in psoriatic DMSCs suggest that these two molecules may involve in the pathogenesis of psoriasis via regulating respective signaling pathway.
Objective: To assess expression levels of phospholipase C-beta 4(PLCB4) and retinol dehydrogenase 10(RDH10)mRNA in dermal mesenchymal stem cells(DMSCs) from lesional skin of psoriatic patients in order to elucidate the cellular and molecular pathomechanisms of psoriasis. Methods: DMSCs from 24 patients with psoriasis vulgaris and 22 normal controls were cultured. Cell phenotypes were identified by flow cytometry, while expression levels of PLCB4 and RDH10 mRNA were determined using real-time fluorescence quantitative PCR. Results: While the morphology of DMSCs was similar between the two groups, expression levels of PLCB4 mRNA in psoriatic DMSCs were 3.35 folds of that in normal controls. Expression levels of RDH10 mRNA in psoriatic DMSCs were 1.17 folds of that in normal controls. Conclusion: The increased expression levels of PLCB4 and RDH10 in psoriatic DMSCs suggest that these two molecules may involve in the pathogenesis of psoriasis via regulating respective signaling pathway.
引文
[1] Zhang K, Liu R, Yin G, et al. Differential cytokine secretion of cultured bone marrow stromal cells from patients with psoriasis and healthy volunteers[J]. Eur J Dermatol, 2010, 20(1):49-53.
[2]刘瑞风,尹国华,张静,等.寻常性银屑病患者骨髓间充质干细胞生物学特性研究(简报)[J].中华微生物学和免疫学杂志,2010,30(3):238.
[3]张开明,李新华.骨髓,银屑病的发病“中枢”?[J].中国皮肤性病学杂志,2004, 18(8):501-503.
[4]王荣,刘瑞风,张永翠,等.不同来源骨髓间充质干细胞对CD34+细胞增殖的影响[J].中国组织工程研究,2012,16(6):973-976.
[5] Salvolini E, Orciani M, Vignini A, et al. Skin-derived mesenchymal stem cells(S-MSCs)induce endothelial cell activation by paracrine mechanisms[J]. Exp Dermatol, 2010, 19(9):848-850.
[6] Liu RF, Wang F, Wang Q, et al. Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells[J]. Genet Mol Res, 2015, 14(4):17758-17767.
[7] Niu X, Chang W, Liu R, et al. Expression of pro-angiogenic genes in mesenchymal stem cells derived from dermis of patients with psoriasis[J]. Int J Dermatol, 2016, 55(5):e280-e288.
[8] Hou R, Yan H, Niu X, et al. Gene expression profile of dermal mesenchymal stem cells from patients with psoriasis[J]. J Eur Acad Dermatol Venereol, 2014, 28(12):1782-1797.
[9] Liu R, Chang W, Wei H, et al. Comparison of the biological characteristics of mesenchymal stem cells derived from bone marrow and skin[J]. Stem Cells Int, 2016, 2016:3658798.
[10]杨元文,刘瑞风,侯瑞霞,等.银屑病患者皮肤间充质干细胞分泌表皮生长因子、转化生长因子-β1水平及意义[J].临床皮肤科杂志,2013,42(6):331-334.
[11] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCtmethod[J]. Methods, 2001, 25(4):402-408.
[12]周愔,刘瑞风,张开明,等.银屑病骨髓间充质干细胞分泌白介素-6和血管内皮生长因子的检测[J].临床皮肤科杂志,2009,38(8):491-493.
[13]刘瑞风,杨元文,赵新程.银屑病患者皮肤间充质干细胞分泌表皮生长因子、干细胞因子、碱性成纤维细胞生长因子、血管内皮生长因子水平检测[J].中国免疫学杂志,2013, 29(10):1046-1049,1063.
[14]李淑,李遇梅.间充质干细胞在皮肤病治疗中的应用[J].临床皮肤科杂志,2016,45(8):614-616.
[15] Alvarez RA, Ghalayini AJ, Xu P, et al. cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4(PLCB4)[J]. Genomics, 1995, 29(1):53-61.
[16] Cocco L, Follo MY, Manzoli L, et al. Phosphoinositide-specific phospholipase C in health and disease[J]. J Lipid Res, 2015, 56(10):1853-1860.
[17]贾圣男,史家欣,李小民.磷脂酰肌醇-3-激酶信号通路调控炎症介质表达机制的研究进展[J].重庆医学,2016,45(18):2567-2570.
[18] Rieder MJ, Green GE, Park SS, et al. A human homeotic transformation resulting from mutations in PLCB4 and GNAI3 causes auriculocondylar syndrome[J]. Am J Hum Genet, 2012, 90(5):907-914.
[19]陈永,管剑龙.风湿性疾病血清维生素A水平下降[J].中国免疫学杂志,2017,33(7):1068-1071.
[20] Ashique AM, May SR, Kane MA, et al. Morphological defects in a novel Rdh10 mutant that has reduced retinoic acid biosynthesis and signaling[J]. Genesis, 2012, 50(5):415-423.
[21]李艳敏,张昌军,董毅飞,等.全反式视黄醛影响内异症内膜基质细胞迁移及视黄醇脱氢酶10 m RNA表达[J].武汉大学学报(医学版),2015,36(4):524-528.
[2]刘瑞风,尹国华,张静,等.寻常性银屑病患者骨髓间充质干细胞生物学特性研究(简报)[J].中华微生物学和免疫学杂志,2010,30(3):238.
[3]张开明,李新华.骨髓,银屑病的发病“中枢”?[J].中国皮肤性病学杂志,2004, 18(8):501-503.
[4]王荣,刘瑞风,张永翠,等.不同来源骨髓间充质干细胞对CD34+细胞增殖的影响[J].中国组织工程研究,2012,16(6):973-976.
[5] Salvolini E, Orciani M, Vignini A, et al. Skin-derived mesenchymal stem cells(S-MSCs)induce endothelial cell activation by paracrine mechanisms[J]. Exp Dermatol, 2010, 19(9):848-850.
[6] Liu RF, Wang F, Wang Q, et al. Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells[J]. Genet Mol Res, 2015, 14(4):17758-17767.
[7] Niu X, Chang W, Liu R, et al. Expression of pro-angiogenic genes in mesenchymal stem cells derived from dermis of patients with psoriasis[J]. Int J Dermatol, 2016, 55(5):e280-e288.
[8] Hou R, Yan H, Niu X, et al. Gene expression profile of dermal mesenchymal stem cells from patients with psoriasis[J]. J Eur Acad Dermatol Venereol, 2014, 28(12):1782-1797.
[9] Liu R, Chang W, Wei H, et al. Comparison of the biological characteristics of mesenchymal stem cells derived from bone marrow and skin[J]. Stem Cells Int, 2016, 2016:3658798.
[10]杨元文,刘瑞风,侯瑞霞,等.银屑病患者皮肤间充质干细胞分泌表皮生长因子、转化生长因子-β1水平及意义[J].临床皮肤科杂志,2013,42(6):331-334.
[11] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCtmethod[J]. Methods, 2001, 25(4):402-408.
[12]周愔,刘瑞风,张开明,等.银屑病骨髓间充质干细胞分泌白介素-6和血管内皮生长因子的检测[J].临床皮肤科杂志,2009,38(8):491-493.
[13]刘瑞风,杨元文,赵新程.银屑病患者皮肤间充质干细胞分泌表皮生长因子、干细胞因子、碱性成纤维细胞生长因子、血管内皮生长因子水平检测[J].中国免疫学杂志,2013, 29(10):1046-1049,1063.
[14]李淑,李遇梅.间充质干细胞在皮肤病治疗中的应用[J].临床皮肤科杂志,2016,45(8):614-616.
[15] Alvarez RA, Ghalayini AJ, Xu P, et al. cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4(PLCB4)[J]. Genomics, 1995, 29(1):53-61.
[16] Cocco L, Follo MY, Manzoli L, et al. Phosphoinositide-specific phospholipase C in health and disease[J]. J Lipid Res, 2015, 56(10):1853-1860.
[17]贾圣男,史家欣,李小民.磷脂酰肌醇-3-激酶信号通路调控炎症介质表达机制的研究进展[J].重庆医学,2016,45(18):2567-2570.
[18] Rieder MJ, Green GE, Park SS, et al. A human homeotic transformation resulting from mutations in PLCB4 and GNAI3 causes auriculocondylar syndrome[J]. Am J Hum Genet, 2012, 90(5):907-914.
[19]陈永,管剑龙.风湿性疾病血清维生素A水平下降[J].中国免疫学杂志,2017,33(7):1068-1071.
[20] Ashique AM, May SR, Kane MA, et al. Morphological defects in a novel Rdh10 mutant that has reduced retinoic acid biosynthesis and signaling[J]. Genesis, 2012, 50(5):415-423.
[21]李艳敏,张昌军,董毅飞,等.全反式视黄醛影响内异症内膜基质细胞迁移及视黄醇脱氢酶10 m RNA表达[J].武汉大学学报(医学版),2015,36(4):524-528.