毛细管电泳法分离9种磺胺类药物
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摘要
本研究建立了毛细管电泳法分离磺胺二甲嘧啶、磺胺氯哒嗪、磺胺吡啶、磺胺醋酰、磺胺噻唑、磺胺甲基嘧啶、磺胺喹恶啉、磺胺甲噻二唑和磺胺间二甲氧嘧啶的方法,测定了滴眼液和兽药药粉中相关磺胺类药物成分含量。毛细管为熔融石英毛细管(75/365μm,40/47cm),运行缓冲液为20mmol/L磷酸盐缓冲溶液(pH 7.5),电压8kV,柱温为20℃,检测波长210nm。9种磺胺类药物分离效果良好。在5～60μg/mL浓度范围内,磺胺类药物峰高和浓度的线性关系良好,相关系数R为0.9922～0.9990。测定滴眼液中磺胺醋酰钠含量以及药粉中磺胺氯哒嗪钠的含量,与标示量基本相符。该方法具有快速、简便、准确度高等特点,适用于磺胺类药物含量的测定。
A new method was established by capillary electrophoresis for the separation of nine sulfonamides,including sulfamethazine,sulfachloropyridazine,sulfapyridine,sulfacetamide,sulfathiazole,sulfamerazine,sulfaquinoxaline,sulfamethizol and sulfadimethoxine.The optimized conditions were that the running buffer was 20 mmol/L phosphates at pH 7.5,separation voltage was 8 kV,temperature was 20℃,detection wavelength was 210 nm.The separation of nine sulfonamides could be completed in 20 minutes with satisfied accuracy.Good linearity was found within the range of 5-60μg/mL and the R ranged from 0.9922 to 0.9990.This method was used to determine the drug content in eye drops and powders and the results were consistent with the labeled amount.It is fast,simple,precise and feasible for the determination of sulfonamides.
A new method was established by capillary electrophoresis for the separation of nine sulfonamides,including sulfamethazine,sulfachloropyridazine,sulfapyridine,sulfacetamide,sulfathiazole,sulfamerazine,sulfaquinoxaline,sulfamethizol and sulfadimethoxine.The optimized conditions were that the running buffer was 20 mmol/L phosphates at pH 7.5,separation voltage was 8 kV,temperature was 20℃,detection wavelength was 210 nm.The separation of nine sulfonamides could be completed in 20 minutes with satisfied accuracy.Good linearity was found within the range of 5-60μg/mL and the R ranged from 0.9922 to 0.9990.This method was used to determine the drug content in eye drops and powders and the results were consistent with the labeled amount.It is fast,simple,precise and feasible for the determination of sulfonamides.
引文
[1]汪涛,杨再福,陈勇航,张姚姚,孙冉冉,薛滢,张梦婷.地表水中磺胺类抗生素的生态风险评价[J].生态环境学报,2016,25(9):1508-1514.
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[12]陈海燕,叶文玲,樊霆.高效液相色谱-荧光检测法测定茶叶中3种磺胺类抗生素.安徽农业大学学报,2017,44(4):580-583.
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[15]刘雪红,李娟,董紫凌,宋平,韩凤丽,马萍.鸡肉中磺胺类药物残留的薄层色谱检测方法.中国畜牧兽医,2007,34(5):31-33.
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[17]陈锡龙.高效液相色谱法测定磺胺间甲氧嘧啶的含量.中国兽药杂志,2017,51(2):16-19.
[18]陈卿卿,金迁,林灵超.固相萃取-高效液相色谱法同时测定饲料中4种磺胺类药物残留.化学分析计量,2017,26(3):84-87.
[19]饶桂维,李成平,王倩,等.快速溶剂萃取-高效液相色谱法测定禽蛋中磺胺嘧啶.化学分析计量,2017,26(1):20-24.
[20]冯爱玲,袁冬梅.食肉中抗菌药磺胺二甲嘧啶的高效液相色谱测定法.辽宁化工,2015,44(11):1414-1415.
[21]唐祝兴,曾祎琨,张卫东,等.高效毛细管电泳-电化学检测滴鼻液中盐酸麻黄碱和磺胺嘧啶.理化检验-化学分册,2008,44(1):71-78.
[22]楚清脆,田秀慧,江莲梅,等.毛细管电泳-安培法测定复方磺胺甲噁唑片中的有效成分.分析化学,2008,36(3):292-296.
[23]谢玉璇,谢天尧,刘秋英,等.毛细管电泳-电导法分离检测磺胺嘧啶、磺胺甲嘧啶和磺胺二甲嘧啶.分析测试学报,2006,25(3):100-102.
[24]张寅艳,靳淑萍,李自成,等.毛细管电泳-电化学检测法测定饲料中的磺胺类药物,分析试验室,2010,29(3):1-5.
[25]代婷婷,林华萍,段婕,等.毛细管区带电泳化学发光法测定食品中残留的磺胺类药物.分析化学,2016,44(5):747-753.
[26]孙彤,张营,翁前锋.反向电压下场放大进样毛细管电泳分析磺胺类药物.广州化工,2016,44(12):130-132.
[27]邱涵,陈素俭,叶飞云.高效毛细管电泳法测定复方磺胺甲口恶唑片中磺胺甲恶唑和甲氧苄啶的含量.中国现代应用药学杂志,2003,20(1):60-61.
[2]马丽丽,郭昌胜,胡伟,等.固相萃取-高效液相色谱-串联质谱法同时测定土壤中氟喹诺酮、四环素和磺胺类抗生素[J].分析化学,2010,38(1):21-26.
[3]Dmitrienko S G,Kochuk E V,Apyari W,et al.Recent advances in sample preparation techniques and methods of sulfonamides detection:a review[J].Analytica Chimica Acta,2014,850:6-25.
[4]Spielmeyer A,Ahlborn J,Hamscher G.Simultaneous determination of 14sulfonamides and tetracyclines in biogas plants by liquid-liquid-extraction and liquid chromatography tandem mass spectrometry[J].Analytical and Bioanalytical Chemistry,2014,406(11):2513-2524.
[5]Papadopoulou M V,Bloomer W D,Rosenzweig H S,et al.Nitrotriazole-and imidazole-based amides and sulfon-amides as antitubercular agents[J].Antimicrobial Agents and Chemotherapy,2014,58(11):6828-6836.
[6]Adrian J,Font H,Diserens J M,et al.Generation of broad specificity antibodies for sulfonamide antibiotics and development of an enzyme-linked immunosorbent assay for the analysis of milk samples[J].Journal of Agricultural and Food Chemistry,2009,57(2):385-394.
[7]Pang G F,Cao Y Z,Fan C L,et al.Liquid chromatography-fluorescence detection for simultaneous analysis of sulfonamide residues in honey[J].Analytical and Bioanalytical Chemistry,2003,376(4):534-541.
[8]王露,汪怡,杭学宇,等.磁性固相萃取-高效液相色谱法测定牛奶中4种磺胺类药物.理化检验-化学分册,2017,53(5):562-565.
[9]陈进军,王元,赵留杰,等.反相高效液相色谱法同时测定青蟹组织中磺胺嘧啶和甲氧苄啶残留.分析科学学报,2017,33(1):67-70.
[10]李玲,赵晓磊,王璇,等.分子印迹固相萃取-高效液相色谱联用检测猪肉中的磺胺类兽药残留.食品工业科技,2017,38(19):249-255.
[11]段婕,李兴华,刘坤,等.高效液相色谱-化学发光法检测牛奶中磺胺类药物残留.分析化学,2017,45(7):1012-1018.
[12]陈海燕,叶文玲,樊霆.高效液相色谱-荧光检测法测定茶叶中3种磺胺类抗生素.安徽农业大学学报,2017,44(4):580-583.
[13]房超,王捍东,王宗元,等.磺胺类药物残留及酶免疫法检测的研究[J].上海畜牧兽医通讯,2004(2):16-17.
[14]Cliquet P,Cox E,Haasnoot W,et al.Extraction procedure for sulfa sulfachloropyridazine in porcine tissues and detection in a sulfonamide-specific enzyme-linked immunosorbent assay[J].Analytica Chimica Acta,2003,494(1):21-28.
[15]刘雪红,李娟,董紫凌,宋平,韩凤丽,马萍.鸡肉中磺胺类药物残留的薄层色谱检测方法.中国畜牧兽医,2007,34(5):31-33.
[16]Kim D H,Choi J O,Kim J,et al.Application of a polymeric solid phase extraction for the analysis of sulphonamides in milk by LC/MS[J].Journal of liquid chromatography&related technologies,2003,26(7):1149-1159.
[17]陈锡龙.高效液相色谱法测定磺胺间甲氧嘧啶的含量.中国兽药杂志,2017,51(2):16-19.
[18]陈卿卿,金迁,林灵超.固相萃取-高效液相色谱法同时测定饲料中4种磺胺类药物残留.化学分析计量,2017,26(3):84-87.
[19]饶桂维,李成平,王倩,等.快速溶剂萃取-高效液相色谱法测定禽蛋中磺胺嘧啶.化学分析计量,2017,26(1):20-24.
[20]冯爱玲,袁冬梅.食肉中抗菌药磺胺二甲嘧啶的高效液相色谱测定法.辽宁化工,2015,44(11):1414-1415.
[21]唐祝兴,曾祎琨,张卫东,等.高效毛细管电泳-电化学检测滴鼻液中盐酸麻黄碱和磺胺嘧啶.理化检验-化学分册,2008,44(1):71-78.
[22]楚清脆,田秀慧,江莲梅,等.毛细管电泳-安培法测定复方磺胺甲噁唑片中的有效成分.分析化学,2008,36(3):292-296.
[23]谢玉璇,谢天尧,刘秋英,等.毛细管电泳-电导法分离检测磺胺嘧啶、磺胺甲嘧啶和磺胺二甲嘧啶.分析测试学报,2006,25(3):100-102.
[24]张寅艳,靳淑萍,李自成,等.毛细管电泳-电化学检测法测定饲料中的磺胺类药物,分析试验室,2010,29(3):1-5.
[25]代婷婷,林华萍,段婕,等.毛细管区带电泳化学发光法测定食品中残留的磺胺类药物.分析化学,2016,44(5):747-753.
[26]孙彤,张营,翁前锋.反向电压下场放大进样毛细管电泳分析磺胺类药物.广州化工,2016,44(12):130-132.
[27]邱涵,陈素俭,叶飞云.高效毛细管电泳法测定复方磺胺甲口恶唑片中磺胺甲恶唑和甲氧苄啶的含量.中国现代应用药学杂志,2003,20(1):60-61.