配位自组装的高效电化学界面对色氨酸的手性识别
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摘要
基于多壁碳纳米管(MWCNTs)和聚精氨酸(PLA)能有效促进电子转移的性能,以及Cu~(2+)修饰的β-环糊精(Cu-β-CD)对色氨酸(Trp)的主客体识别和富集能力,本研究采用MWCNTs、PLA和Cu-β-CD构建手性传感器,得到Cu-β-CD/PLA/MWCNTs复合膜,并用示差脉冲伏安法(DPV)对色氨酸(Trp)异构体进行手性识别和检测。结果表明,该手性传感器对Trp异构体产生显著的电流差,并且对L-Trp的电流响应高于D-Trp,两者分离系数可达3.37,并在1.0×10~(-6)～5.5×10~(-5) mol·L~(-1)浓度范围内呈现良好的线性相关,检出限(S/N=3)可达3.3×10~(-7) mol·L~(-1)。所制备的传感器响应快速、识别效率高,在手性分子识别领域具有潜在的应用价值。
Construction of convenient yet effective systems for isomer discrimination is of vital significance for medical science and biochemistry.In this work,an effective chiral sensing platform for chiral recognition of tryptophan(Trp)isomers was fabricated via Cu~(2+)-modifiedβ-cyclodextrin(Cu-β-CD)selfassembled on poly(L-Arginine)/multiwalled carbon nanotubes(Cu-β-CD/PLA/MWCNTs).The experimental results indicate that the sensor exhibits excellent chiral recognition toward L-Trp as compared to D-Trp with the separation coefficient of 3.37.And it suggests that both MWCNTs and PLA could facilitate the electron transfer,as well as the host-guest recognition and enrichment ability of Cu-β-CD for Trp.Meanwhile,the developed sensor displays a good linear range from 1.0×10~(-6) mol/L to 5.5×10~(-5) mol/L with the limit of detection of 3.3×10~(-7) mol/L(S/N=3)for L-Trp and D-Trp.The proposed sensor provides its potential applications in the isomers recognition field with the advantages of simple operation,rapid detection and response.
Construction of convenient yet effective systems for isomer discrimination is of vital significance for medical science and biochemistry.In this work,an effective chiral sensing platform for chiral recognition of tryptophan(Trp)isomers was fabricated via Cu~(2+)-modifiedβ-cyclodextrin(Cu-β-CD)selfassembled on poly(L-Arginine)/multiwalled carbon nanotubes(Cu-β-CD/PLA/MWCNTs).The experimental results indicate that the sensor exhibits excellent chiral recognition toward L-Trp as compared to D-Trp with the separation coefficient of 3.37.And it suggests that both MWCNTs and PLA could facilitate the electron transfer,as well as the host-guest recognition and enrichment ability of Cu-β-CD for Trp.Meanwhile,the developed sensor displays a good linear range from 1.0×10~(-6) mol/L to 5.5×10~(-5) mol/L with the limit of detection of 3.3×10~(-7) mol/L(S/N=3)for L-Trp and D-Trp.The proposed sensor provides its potential applications in the isomers recognition field with the advantages of simple operation,rapid detection and response.
引文
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[2]Gopal R,Seo C H,Song P I,Park Y.Amino Acids,2013,44(2):645.
[3]Raoof J B,Ojani R,Karimi-Maleh H.Electroanalysis,2008,20(11):1259.
[4]Liang Y D,Song J F.Journal of Pharmaceutical and Biomedical Analysis,2005,38(1):100.
[5]Zhang Q L,Wang D L,Zhang M Y,Zhao Y L,Yu Z G.Journal of Chromatography B,2017,1041:175.
[6]Miller G D,Johnson J A,Miller B S.Analytical Chemistry,1956,28:884.
[7]Ou J,Tao Y X,Xue J J,Kong Y,Dai J Y,Deng L H.Electrochemistry Communications,2015,57:5.
[8]Xu C X,Huang K,Fan Y,Wu Z W,Li J,Gan T.Materials Science and Engineering C,2012,32(4):969.
[9]Matsunaga M,Ueno T,Nakanishi T,Osaka T.Electrochemistry Communications,2008,10:1844.
[10]Yu L Y,Liu Q,Wu X W,Jiang X Y,Yu J G,Chen X Q.RSC Advances,2015,5:98020.
[11]Feng W L,Liu C,Lu S Y,Zhang C Y,Zhu X H,Liang Y,Nan J M.Microchim Acta,2014,181:501.
[12]Wang F,Gong W C,Wang L L,Chen Z L.Analytical Biochemistry,2016,492:30.
[13]Guo D M,Huang Y H,Chen C,Chen Y,Fu Y Z.New Journal of Chemistry,2014,38(12):5880.
[14]Wang C Y,Shao X Q,Liu Q X,Qu Q S,Yang G J,Hu X Y.Journal of Pharmaceutical and Biomedical Analysis,2006,42(2):237.
[15]Juvancz Z,Kendrovics R B,Ivanyi R,Szente L.Electrophoresis,2008,29(8):1701.
[16]Tao Y X,Dai J Y,Kong Y,Sha Y.Analytical Chemistry,2014,86(12):2633.
[17]Ou J,Zhu Y H,Kong Y,Ma J F.Electrochemistry Communications,2015,60:60.
[18]Zaidi S A.Biosensors and Bioelectronics,2017,94:714.
[19]Li Z L,Mo Z,Guo R B,Meng S J,Wang R J,Gao H H,Niu X H.Analytical Methods,2017,9(35):5149.
[20]Xu J J,Wang Q H,Xuan C Z,Xia Q,Lin X,Fu Y Z.Electroanalysis,2016,28(4):868.