PEG模拟干旱胁迫下花椰菜种质资源萌发特性及抗旱性评价
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摘要
为鉴定和筛选花椰菜耐旱种质资源,以12个花椰菜种质资源为试验材料,测定不同浓度PEG[0(CK)、100、150和200 g·L~(-1)]模拟干旱胁迫对花椰菜的相对发芽率(RGP)、相对发芽势(RGR)、相对发芽指数(RGI)、相对活力指数(RVI)、相对苗高(RSH)和相对根长(RRL)等6个指标的影响,并采用隶属函数法进行抗旱性的综合评价。结果表明,100 g·L~(-1)PEG胁迫对花椰菜种子萌发的影响不大,150～200 g·L~(-1)PEG胁迫则显著抑制花椰菜种子萌发。100～150 g·L~(-1)PEG胁迫对RRL具有一定的促进作用,而150～200 g·L~(-1)PEG胁迫则会明显抑制RSH,表明150～200 g·L~(-1)PEG可作为花椰菜萌发期抗旱性筛选的适宜浓度。基于RGP、RGR、RGI、RVI、RSH和RRL 6个指标,通过隶属函数法综合评价发现,12个花椰菜材料的抗旱性依次表现为P3>P6>A1>P8>P7>P2>P1>P4>P5>A2>A3>P9。本研究结果为花椰菜抗旱品种选育提供了一定的理论依据。
Twelve cauliflower materials were used to evaluate their drought tolerance by examining their relative germination percentage( RGP),relative germination rate( RGR),relative germination index( RGI),relative vigor index( RVI),relative seedling height( RSH) and relative root length( RRL) under different PEG concentrations including 0( CK),100,150 and 200 g·L~(-1) by method of subordinate function. The results showed that,100 g·L~(-1) PEG stress presented lower effect on seed germinating,while 150 ～ 200 g·L~(-1) PEG stress significantly inhibited seed germinating. The 100 ～ 150 g·L~(-1) PEG stress had a promoting effect on RRL,while 150 ～ 200 g·L~(-1) PEG stress presented significant inhibition on RSH. Therefore,150 ～ 200 g·L~(-1) PEG was most suitable for screening drought resistance of cauliflower at germination stage. According to comprehensive evaluation of subordinate function method based on RGP,RGR,RGI,RVI,RSH and RRL,the drought resistance of the 12 cauliflower materials followed the order of P3>P6>A1>P8>P7>P2>P1>P4>P5>A2>A3>P9. These results provide theoretical evidence for breeding of drought resistance in cauliflower.
Twelve cauliflower materials were used to evaluate their drought tolerance by examining their relative germination percentage( RGP),relative germination rate( RGR),relative germination index( RGI),relative vigor index( RVI),relative seedling height( RSH) and relative root length( RRL) under different PEG concentrations including 0( CK),100,150 and 200 g·L~(-1) by method of subordinate function. The results showed that,100 g·L~(-1) PEG stress presented lower effect on seed germinating,while 150 ～ 200 g·L~(-1) PEG stress significantly inhibited seed germinating. The 100 ～ 150 g·L~(-1) PEG stress had a promoting effect on RRL,while 150 ～ 200 g·L~(-1) PEG stress presented significant inhibition on RSH. Therefore,150 ～ 200 g·L~(-1) PEG was most suitable for screening drought resistance of cauliflower at germination stage. According to comprehensive evaluation of subordinate function method based on RGP,RGR,RGI,RVI,RSH and RRL,the drought resistance of the 12 cauliflower materials followed the order of P3>P6>A1>P8>P7>P2>P1>P4>P5>A2>A3>P9. These results provide theoretical evidence for breeding of drought resistance in cauliflower.
引文
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[24]吴奇,周宇飞,高悦,张姣,陈冰嬬,许文娟,黄瑞冬.不同高粱品种萌发期抗旱性筛选与鉴定[J].作物学报,2016,42(8):1233-1246
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[26]董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355
[27]裴帅帅,尹美强,温银元,黄明镜,张彬,郭平毅,王玉国,原向阳.不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].核农学报,2014,28(10):1897-1904
[28]陈致富,李勤菲,张永晶,崔艺馨,许汪洁,贺亚军,万华方,李晓荣,钱伟.白菜型油菜品种萌发期的抗旱性鉴定与筛选[J].植物遗传资源学报,2015,16(1):15-22
[29]张霞,谢小玉.PEG胁下甘蓝型油菜种子萌发期抗旱鉴定指标的研究[J].西北农业学报,2012,21(2):72-77
[30]张宇君,赵丽丽,王普昶,陈超,康芙蓉.燕麦萌发期抗旱指标体系构建及综合评价[J].核农学报,2017,31(11):2236-2242
[2]Wu X J,Zeng F R,Zhang G P.PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality[J].Plant Growth Regulation,2017,81:243-252
[3]闫春娟,宋书宏,王文斌,王昌陵.大豆耐旱种质的鉴定[J].大豆科学,2015,34(1):163-167
[4]Yousef,E A A,Lampei C,Schmid K J.Evaluation of cauliflower genebank accessions under organic and conventional cultivation in Southern Germany[J].Euphytica,2015,201:389-400
[5]陈国菊,杨暹,吴筱颖.干旱胁迫对花椰菜叶片细胞保护酶及超微结构的影响[J].中国蔬菜,2002,1(2):8-11
[6]汪炳良,徐敏,史庆华,曹家树.高温胁迫对早熟花椰菜叶片抗氧化系统和叶绿素及其荧光参数的影响[J].中国农业科学,2004,37(8):1245-1250
[7]王丽君,张国斌,周亚婷,周德霞,杨伟,杨海兴,郁继华.外源水杨酸对干旱胁迫下花椰菜叶片抗氧化作用的影响[J].中国农学通报,2014,30(25):91-96
[8]Wu H L,Wu X L,Li Z H,Duan L S,Zhang M C.Physiological evaluation of drought stress tolerance and recovery in cauliower(Brassica oleracea L.)seedlings treated with methyl jasmonate and coronatine[J].Journal of Plant Growth Regulation,2012,31:113-123
[9]Hadi F,Ayaz M,Ali S,Shafiq M,Ullah R,Jan A U.Comparative effect of polyethylene glycol and mannitol induced drought on growth(in vitro)of canola(Brassica napus),cauliflower(Brassica oleracea)and tomato(Lycopersicon esculentum)seedlings[J].International Journal of Biosciences,2014,4(9):34-41
[10]杨瑰丽,杨美娜,李帅良,曲志恒,黄明,陈志强,王慧.水稻萌芽期抗旱指标筛选与抗旱性综合评价[J].华南农业大学学报,2015,36(2):1-5
[11]Li J Z,Chen Y P,Teng K Q,Qin L Z,Du Y X,Zhang J,Zhao QZ.Rice leaf heterogeneity in chlorophyll fluorescence parameters under short-term osmotic stress[J].Biologia Plantarum,2015,59(1):187-192
[12]王阳,杨永志,张玲,侯佳明,陈冰嬬,高鸣,胡喜连,李继洪,李淑杰,高士杰.玉米黄改系材料萌芽期耐旱性鉴定与评价[J].玉米科学,2016,24(3):1-6
[13]Shen J,Xu G X,Zheng H Q.Apoplastic barrier development and water transport in Zea mays seedling roots under salt and osmotic stresses[J].Protoplasma,2015,252:173-180
[14]成锴,苏晓慧,栗建枝,赵太存,王国梁,李洪.PEG-6000胁迫下玉米品种萌发期抗旱性鉴定与评价[J].玉米科学,2017,25(5):85-90
[15]崔宏亮,姚庆,李利民,苗昊翠.PEG模拟干旱胁迫下花生品种萌发特性与抗旱性评价[J].核农学报,2017,31(7):1412-1418
[16]刘永惠,詹成芳,沈一,陈志德.不同花生品种(系)萌发期抗旱性鉴定评价[J].植物遗传资源学报,2016,17(2):233-238
[17]李玲,沈宝宇,张天静,杨涛,刘荣,宗绪晓.豌豆种质资源芽期耐旱性评价及耐旱种质筛选[J].植物遗传资源学报,2017,18(4):778-785
[18]徐小玉,张凤银,李俊芳.PEG渗透胁迫下12个豇豆品种萌芽期抗旱性评价[J].东北农业大学学报,2016,47(1):15-20
[19]李娜,罗俊杰,张仁陟,刘新星.持续模拟干旱胁迫对胡麻萌发特性影响及品种抗旱性评价研究[J].核农学报,2016,30(2):379-387
[20]王宗胜,张建平,米君,李闻娟.PEG胁迫下不同胡麻品种种子萌发期抗旱性鉴定[J].干旱地区农业研究,2016,34(4):118-124
[21]George S,Minhas N M,Jatoi S A,Siddiqui S U,Ghafoor A.Impact of polyethylene glycol on proline and membrane stability index for water stress regime in tomato(Solanum Lycopersicum)[J].Pakistan Journal of Botany,2015,47(3):835-844
[22]黎冬华,刘文萍,张艳欣,王林海,危文亮,高媛,丁霞,王蕾,张秀荣.芝麻耐旱性的鉴定方法及关联分析[J].作物学报,2013,39(8):1425-1433
[23]翟荣荣,叶胜海,余鹏,赵小燕,金庆生,张小明.浙江省晚粳稻品种对PEG胁迫的反应和耐旱性鉴定[J].核农学报,2015,29(10):1991-1997
[24]吴奇,周宇飞,高悦,张姣,陈冰嬬,许文娟,黄瑞冬.不同高粱品种萌发期抗旱性筛选与鉴定[J].作物学报,2016,42(8):1233-1246
[25]李培英,孙宗玖,阿不来提.PEG模拟干旱胁迫下29份偃麦草种质种子萌发期抗旱性评价[J].中国草地学报,2010,32(1):32-39
[26]董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355
[27]裴帅帅,尹美强,温银元,黄明镜,张彬,郭平毅,王玉国,原向阳.不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].核农学报,2014,28(10):1897-1904
[28]陈致富,李勤菲,张永晶,崔艺馨,许汪洁,贺亚军,万华方,李晓荣,钱伟.白菜型油菜品种萌发期的抗旱性鉴定与筛选[J].植物遗传资源学报,2015,16(1):15-22
[29]张霞,谢小玉.PEG胁下甘蓝型油菜种子萌发期抗旱鉴定指标的研究[J].西北农业学报,2012,21(2):72-77
[30]张宇君,赵丽丽,王普昶,陈超,康芙蓉.燕麦萌发期抗旱指标体系构建及综合评价[J].核农学报,2017,31(11):2236-2242