颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及影响因素研究
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摘要
颗粒体病毒(CnmeGV)为感染稻纵卷叶螟的专性杆状病毒。为进一步明确CnmeGV对稻纵卷叶螟的感染及影响因素,利用扫描电镜分析CnmeGV在宿主组织内的感染致病过程,生物测定探明病毒浓度、虫龄、温度及紫外光照射等因素对CnmeGV感染活性的影响。结果表明:CnmeGV主要感染稻纵卷叶螟幼虫脂肪体细胞,表现出颗粒体病毒Ⅱ型特征。CnmeGV经过较长侵染周期造成系统性感染,接种5d呈现明显的感病症状。幼虫感病率与CnmeGV含量呈非线性相关,高龄幼虫免疫力显著增强。CnmeGV对温度、紫外光照射及pH具有一定的抗逆性,pH值5～8、温度50℃以下对CnmeGV的感染活性影响较小,紫外光照射1h幼虫感病率为67.57%。CnmeGV通过感染脂肪体细胞造成稻纵卷叶螟系统性感染;合理选择病毒浓度和感染虫龄、减少高温及紫外光照射有利于提高病毒的感染活性。
Cnaphalocrocis medinalis granulovirus (CnmeGV),apathogenic baculovirus to C.medinalis,is a potential pathogen for the pest control.The purpose of this paper is to explore the infectivity and influence factors of CnmeGV to larvae of C.medinalis.The results showed that systematic infection of CnmeGV to larva of C.medinalis underwent a relative long period.Virus-infected larvae exhibited distinct symptoms about 5 dafter incubation of CnmeGV,while massive occlusion bodies (OB) were detected in the tissues.The fat body cells of larva were the main tissues to be infected by CnmeGV,consisted with the typical broad tissue tropism of the typeⅡ GVs.Nonlinear correlation was observed between virus concentration and larva infection rate.Immunity of high instar larvae was enhanced significantly under low concentration of CnmeGV.A suitable selection of virus dosage and larva instar might improve the infectivity of CnmeGV.CnmeGV exhibited some resistance to the environment factors,such as temperature,radiation and pH,perhaps due to the special structure of OB.Little impaction was shown to the infectivity of CnmeGV when the pH value was between 5 and 8 and the temperature was under 50 ℃.Reducing ultraviolet radiation might contribute to keeping the infectivity of CnmeGV.
Cnaphalocrocis medinalis granulovirus (CnmeGV),apathogenic baculovirus to C.medinalis,is a potential pathogen for the pest control.The purpose of this paper is to explore the infectivity and influence factors of CnmeGV to larvae of C.medinalis.The results showed that systematic infection of CnmeGV to larva of C.medinalis underwent a relative long period.Virus-infected larvae exhibited distinct symptoms about 5 dafter incubation of CnmeGV,while massive occlusion bodies (OB) were detected in the tissues.The fat body cells of larva were the main tissues to be infected by CnmeGV,consisted with the typical broad tissue tropism of the typeⅡ GVs.Nonlinear correlation was observed between virus concentration and larva infection rate.Immunity of high instar larvae was enhanced significantly under low concentration of CnmeGV.A suitable selection of virus dosage and larva instar might improve the infectivity of CnmeGV.CnmeGV exhibited some resistance to the environment factors,such as temperature,radiation and pH,perhaps due to the special structure of OB.Little impaction was shown to the infectivity of CnmeGV when the pH value was between 5 and 8 and the temperature was under 50 ℃.Reducing ultraviolet radiation might contribute to keeping the infectivity of CnmeGV.
引文
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[4]谭荣荣,王友平,毛迎新,等.理化因子对茶尺蠖核型多角体病毒活性的影响[J].浙江农业科学,2016,57(12):2030-2032.
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[11]徐健,李传明,韩光杰,等.颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响[J].江苏农业学报,2018,34(1):29-33.
[12]XU J,LI C M,YANG Y J,et al.Growth and reproduction of artificially fed Cnaphalocrocis medinalis[J].Rice Science,2012,19(3):247-251.
[13]CORY J S,FRANKLIN M T.Evolution and the microbial control of insects[J].Evolutionary Applications,2012,5(5):455-469.
[14]GOLDBERG A V,ROMANOWSKI V,FEDERICI B A,et al.Effects of the Epap granulovirus on its host,Epinotia aporema(Lepidoptera:Tortricidae)[J].Journal of Invertebrate Pathology,2002,80:148-159.
[15]TWEETEN K A,BULLA JR L A,CONSIGLI R A.Applied and molecular aspects of insect granulosis viruses[J].Micobiological Reviews,1981,45(3):379-408.
[16]PASSARELLI A L.Barriers to success:how baculoviruses establish efficient systemic infections[J].Virology.2011,411(2):383-392.
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[18]孙修炼,胡志红,彭辉银.杆状病毒生态学研究进展[J].中国病毒学,2006,21(2):200-206.
[19]ERLANDSON M.Insect pest control by viruses[M].Oxford:Academic Press,2008.
[20]高继平,隋阳辉,张文忠,等.水稻灌浆期冠层温度对植株生理性状及稻米品质的影响[J].中国水稻科学,2015(5):501-510.
[21]LACEY L A,ARTHURS S P.New method for testing solar sensitivity of commercial formulations of the granulovirus of codling moth(Cydia pomonella,Tortricidae:Lepidoptera)[J].Journal of Invertebrate Pathology,2005,90:85-90.
[22]樊江斌,吴正伟,刘国锋,等.一种增强苹果蠹蛾颗粒体病毒田问防效方法的建立[J].生物安全学报,2015,24(4):310-314.
[2]彭建新.杆状病毒分子生物学[M].武汉:华中师范大学出版社,2000.
[3]SZEWCZYK B,HOYOS-CARVAJAL L,PALUSZEK M,et al.Baculoviruses:re-emerging biopesticides[J].Biotechnology Advances,2006,24(2):143-160.
[4]谭荣荣,王友平,毛迎新,等.理化因子对茶尺蠖核型多角体病毒活性的影响[J].浙江农业科学,2016,57(12):2030-2032.
[5]秦启联,程清泉,张继红,等.昆虫病毒生物杀虫剂产业化及其展望[J].中国生物防治学报,2012,28(2):157-164.
[6]庞义,濑涌流,刘炬,等.稻纵卷叶螟颗粒体病毒[J].微生物学通报,1981(3):103-104.
[7]张珊,贾茜雯,孙士锋,等.一株稻纵卷叶螟颗粒体病毒的系统发育和流行病学调查[J].环境昆虫学报,2014,36(5):756-776.
[8]XU J,LI C M,HAN G J,et al.Cnaphalocrocis medinalis(Guenée)Granulovirus,apotential microbial agent against the rice leaffolder Cnaphalocrocis medinalis Güenée(Lepidoptera:Pyralidae)[J].Egyptian Journal Biological Pest Control,2016,26(4):675-681.
[9]刘琴,徐健,王艳,等.CmGV与Bt对稻纵卷叶螟幼虫的协同作用研究[J].扬州大学学报(农业与自然科学版),2013,34(4):89-93.
[10]HAN G J,XU J,LIU Q,et al.Genome of Cnaphalocrocis medinalis granulovirus,the first crambidae-infecting betabaculovirus isolated from rice leaffolder to sequenced[J].PLoS ONE,2016,11(2):e0147882.
[11]徐健,李传明,韩光杰,等.颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响[J].江苏农业学报,2018,34(1):29-33.
[12]XU J,LI C M,YANG Y J,et al.Growth and reproduction of artificially fed Cnaphalocrocis medinalis[J].Rice Science,2012,19(3):247-251.
[13]CORY J S,FRANKLIN M T.Evolution and the microbial control of insects[J].Evolutionary Applications,2012,5(5):455-469.
[14]GOLDBERG A V,ROMANOWSKI V,FEDERICI B A,et al.Effects of the Epap granulovirus on its host,Epinotia aporema(Lepidoptera:Tortricidae)[J].Journal of Invertebrate Pathology,2002,80:148-159.
[15]TWEETEN K A,BULLA JR L A,CONSIGLI R A.Applied and molecular aspects of insect granulosis viruses[J].Micobiological Reviews,1981,45(3):379-408.
[16]PASSARELLI A L.Barriers to success:how baculoviruses establish efficient systemic infections[J].Virology.2011,411(2):383-392.
[17]FEDERICI B A,MILLER L K.The Baculoviruses[M].New York:Plenum Press,1997.
[18]孙修炼,胡志红,彭辉银.杆状病毒生态学研究进展[J].中国病毒学,2006,21(2):200-206.
[19]ERLANDSON M.Insect pest control by viruses[M].Oxford:Academic Press,2008.
[20]高继平,隋阳辉,张文忠,等.水稻灌浆期冠层温度对植株生理性状及稻米品质的影响[J].中国水稻科学,2015(5):501-510.
[21]LACEY L A,ARTHURS S P.New method for testing solar sensitivity of commercial formulations of the granulovirus of codling moth(Cydia pomonella,Tortricidae:Lepidoptera)[J].Journal of Invertebrate Pathology,2005,90:85-90.
[22]樊江斌,吴正伟,刘国锋,等.一种增强苹果蠹蛾颗粒体病毒田问防效方法的建立[J].生物安全学报,2015,24(4):310-314.