尖裸鲤5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析
|
摘要
为了了解尖裸鲤的生化遗传特性,丰富尖裸鲤种质资源研究的内容,通过配制不连续浓度聚丙烯酰胺凝胶,采用聚丙烯胺凝胶垂直板电泳对尖裸鲤5种组织(心脏、眼睛晶状体、肌肉、肝脏和肾脏)中的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)进行了电泳。结果表明:所测样本鱼心脏、眼睛晶状体、肌肉、肝脏和肾脏中的LDH酶带数分别为:12～16条,17～18条,6～13条,6～10条和12～17条; MDH酶带数分别为3条,4条,3～5条,2条和4～7条。LDH在尖裸鲤厌氧器官(骨骼肌和肝脏)中表达的酶带数总体少于非厌氧器官(心脏和肾脏)。尖裸鲤的MDH分为上清液型(s-MDH)和线粒体型(mMDH)。尖裸鲤的LDH和MDH同工酶系统具有组织特异性,尖裸鲤相对较多的LDH酶带数可能与其独特的栖息环境和A亚基与B亚基的变异共同作用有关。本研究可从生化遗传角度为尖裸鲤的资源保护和利用提供一定理论依据。
The purpose was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Oxygymnocypris stewartii. By preparing concentration gradient polyacrylamide and using the vertical polyacrylamide gel electrophoresis,lactate dehydrogenase(LDH) and malate dehydrogenase(MDH) from five tissues(heart,crystalline lens,muscle,liver and kidney) of Oxygymnocypris stewartii were preliminarily studied. The results showed that: the band numbers of LDH isozymes from heart,Crystalline lens,muscle,liver and kidney were 12-16,17-18,6-13,6-10 and 12-17 respectively,and MDH isozymes 3,4,3-5,2 and 4-7. The band numbers of LDH isozymes expressed in anaerobic organs such as muscle and liver were less than that in non-anaerobic organs such as heart and kidney. MDH isozymes in Oxygymnocypris stewartii were divided into supernate type and mitochondria type. LDH isozymes and MDH showed tissue specificity. More bands of LDH isozymes in Oxygymnocypris stewartii might be related to the interaction between it's unique habitant and the variation of subunit A and subunit B. The results could provide theoretical basis for the conservation and utilization of Oxygymnocypris stewartii from the biochemical genetics level.
The purpose was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Oxygymnocypris stewartii. By preparing concentration gradient polyacrylamide and using the vertical polyacrylamide gel electrophoresis,lactate dehydrogenase(LDH) and malate dehydrogenase(MDH) from five tissues(heart,crystalline lens,muscle,liver and kidney) of Oxygymnocypris stewartii were preliminarily studied. The results showed that: the band numbers of LDH isozymes from heart,Crystalline lens,muscle,liver and kidney were 12-16,17-18,6-13,6-10 and 12-17 respectively,and MDH isozymes 3,4,3-5,2 and 4-7. The band numbers of LDH isozymes expressed in anaerobic organs such as muscle and liver were less than that in non-anaerobic organs such as heart and kidney. MDH isozymes in Oxygymnocypris stewartii were divided into supernate type and mitochondria type. LDH isozymes and MDH showed tissue specificity. More bands of LDH isozymes in Oxygymnocypris stewartii might be related to the interaction between it's unique habitant and the variation of subunit A and subunit B. The results could provide theoretical basis for the conservation and utilization of Oxygymnocypris stewartii from the biochemical genetics level.
引文
[1]西藏自治区水产局.西藏鱼类及其资源[M].北京:中国农业出版社,1995:114-115.
[2]武云飞,吴翠珍.青藏高原鱼类西藏鱼类及其资源[M].成都:四川科学技术出版社,1991:463-465.
[3]洛桑,张强英,丹增达瓦,等.拉萨河尖裸鲤(Oxygymnocypris stewartii)肌肉营养组成与分析评价[J].西藏大学学报:自然科学版,2014,29(1):8-12,53.
[4]曹文宣,陈宜瑜,武云飞,等.裂腹鱼类的起源和演化及其与青藏高原的隆起关系[M].北京:科学出版社,1981:118-130.
[5]武云飞,康斌,门强,等.西藏鱼类染色体多样性的研究[J].动物学研究,1999,29(4):18-19,21-24.
[6]许静,谢从新,邵俭,等.雅鲁藏布江尖裸鲤胚胎和仔稚鱼发育研究[J].水生态学杂志,2011,30(2):86-95.
[7]张涛,陈建武,张林,等.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2017,48(12):2281-2287.
[8]余来宁,夏小平,杨东,等.草鱼♀×鱤♂杂交F1代同工酶和蛋白质的电泳分析[J].安徽农业科学,2014,42(30):10573-10575.
[9]张娟,王红叶,蔡焰值,等.中华倒刺鲃同工酶组织特异性研究[J].湖北农业科学,2011,50(24):5206-5210.
[10]杨从戎,苗亮,李明云.黑鲷不同组织的9种同工酶谱[J].生物学杂志,2014,31(3):38-42.
[11]赵田田,蒲宗旺,岳兴建.花斑副沙鳅七种同工酶的组织特异性表达[J].湖北农业科学,2017,56(14):2736-2742.
[12]熊全沫.鱼类同工酶分析(上)[J].遗传,1992,16(4):372-378
[13]张龙岗,刘羽清,朱树人,等.乌鳢和杂交鳢4种同工酶的比较研究[J].长江大学学报,2017,14(18):33-36.
[14]余敏,杨正荣,曾琳,等.云南高背鲫鱼不同组织同工酶分析[J].动物学杂志,2006,41(6):97-103.
[15]贺刚,方春林,王伟萍,等.大口鲇(♀)×怀头鲇(♂)杂种F_1的形态学及遗传学研究[J].湖北农业科学,2011,50(23):4916-4919.
[16]刘磊,张晨捷,彭士明,等.银鲳不同组织中4种同工酶的表达差异[J].海洋渔业,2017,39(1):68-75.
[17]张燕萍,徐先栋,章海鑫,等.黄尾鲴6种同工酶的组织特异性研究[J].中国农学通报,2016,32(35):10-14.
[18]杨太有,李仲辉,张西瑞,等.黄河鲤鱼乳酸脱氢酶同工酶的研究[J].河南师范大学学报,1995,23(2):65-68.
[19]李太平,赫广春,赵凯,等.青海湖裸鲤乳酸脱氢酶的研究[J].黑龙江畜牧兽医,2001(10):7-8.
[20]祁得林,李军祥.青海湖裸鲤血清过氧化物酶多态性初步研究[J].淡水渔业,2002,32(5):57-58.
[21]孟鹏,史建全,祁洪芳,等.青海湖裸鲤同工酶表达的组织特异性分析[J].海洋水产研究,2008,29(5):112-119.
[22]张才骏,张武学,李清军,等.青海湖裸鲤乳酸脱氢酶同工酶酶谱[J].青海畜牧兽医学院学报,1992,9(1):7-11.
[23]张武学,张才骏,李军祥,等.青海湖裸鲤乳酸脱氢酶同工酶的研究[J].青海畜牧兽医杂志,1994,24(3):9-12.
[24]罗莉中,王春元.金鱼(Carassius auratus L.)同工酶的发生遗传学研究Ⅱ.金鱼胚胎发育不同时期乳酸脱氢酶同工酶分析[J].遗传学报,1984,11(6):457-493.
[25]朱蓝菲,陈湘粦,王祖熊. 20种鲤科鱼类同工酶的表型分析及有关进化问题的探讨[J].水产学报,1983,7(2):145-152.
[26]祁得林.青海湖裸鲤和鲤鱼组织乳酸脱氢酶同工酶比较研究[J].青海大学学报:自然科学版,2003,21(6):1-3.
[27]魏乐.青海湖裸鲤、鲤鱼的血红蛋白的电泳研究[J].青海师范大学学报:自然科学版,2000(2):39-43.
[28]秦桂香,朱海梅,李谕,等.青海湖裸鲤过氧化氢酶活性及电泳分析[J].黑龙江畜牧兽医报,2014,7:181-182
[29] Chen Y F,He D K,Chen Y Y. Electrophoretic analysis of isozymes and discussion about species differentiation in three species of Genus gymnocypris[J]. Zoological Research,2001,22(1):9-19.
[30]胡思玉,彭垠,赵海涛,等.四川裂腹鱼同工酶研究[J].湖北农业科学,2015,54(18):4540-4543.
[2]武云飞,吴翠珍.青藏高原鱼类西藏鱼类及其资源[M].成都:四川科学技术出版社,1991:463-465.
[3]洛桑,张强英,丹增达瓦,等.拉萨河尖裸鲤(Oxygymnocypris stewartii)肌肉营养组成与分析评价[J].西藏大学学报:自然科学版,2014,29(1):8-12,53.
[4]曹文宣,陈宜瑜,武云飞,等.裂腹鱼类的起源和演化及其与青藏高原的隆起关系[M].北京:科学出版社,1981:118-130.
[5]武云飞,康斌,门强,等.西藏鱼类染色体多样性的研究[J].动物学研究,1999,29(4):18-19,21-24.
[6]许静,谢从新,邵俭,等.雅鲁藏布江尖裸鲤胚胎和仔稚鱼发育研究[J].水生态学杂志,2011,30(2):86-95.
[7]张涛,陈建武,张林,等.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2017,48(12):2281-2287.
[8]余来宁,夏小平,杨东,等.草鱼♀×鱤♂杂交F1代同工酶和蛋白质的电泳分析[J].安徽农业科学,2014,42(30):10573-10575.
[9]张娟,王红叶,蔡焰值,等.中华倒刺鲃同工酶组织特异性研究[J].湖北农业科学,2011,50(24):5206-5210.
[10]杨从戎,苗亮,李明云.黑鲷不同组织的9种同工酶谱[J].生物学杂志,2014,31(3):38-42.
[11]赵田田,蒲宗旺,岳兴建.花斑副沙鳅七种同工酶的组织特异性表达[J].湖北农业科学,2017,56(14):2736-2742.
[12]熊全沫.鱼类同工酶分析(上)[J].遗传,1992,16(4):372-378
[13]张龙岗,刘羽清,朱树人,等.乌鳢和杂交鳢4种同工酶的比较研究[J].长江大学学报,2017,14(18):33-36.
[14]余敏,杨正荣,曾琳,等.云南高背鲫鱼不同组织同工酶分析[J].动物学杂志,2006,41(6):97-103.
[15]贺刚,方春林,王伟萍,等.大口鲇(♀)×怀头鲇(♂)杂种F_1的形态学及遗传学研究[J].湖北农业科学,2011,50(23):4916-4919.
[16]刘磊,张晨捷,彭士明,等.银鲳不同组织中4种同工酶的表达差异[J].海洋渔业,2017,39(1):68-75.
[17]张燕萍,徐先栋,章海鑫,等.黄尾鲴6种同工酶的组织特异性研究[J].中国农学通报,2016,32(35):10-14.
[18]杨太有,李仲辉,张西瑞,等.黄河鲤鱼乳酸脱氢酶同工酶的研究[J].河南师范大学学报,1995,23(2):65-68.
[19]李太平,赫广春,赵凯,等.青海湖裸鲤乳酸脱氢酶的研究[J].黑龙江畜牧兽医,2001(10):7-8.
[20]祁得林,李军祥.青海湖裸鲤血清过氧化物酶多态性初步研究[J].淡水渔业,2002,32(5):57-58.
[21]孟鹏,史建全,祁洪芳,等.青海湖裸鲤同工酶表达的组织特异性分析[J].海洋水产研究,2008,29(5):112-119.
[22]张才骏,张武学,李清军,等.青海湖裸鲤乳酸脱氢酶同工酶酶谱[J].青海畜牧兽医学院学报,1992,9(1):7-11.
[23]张武学,张才骏,李军祥,等.青海湖裸鲤乳酸脱氢酶同工酶的研究[J].青海畜牧兽医杂志,1994,24(3):9-12.
[24]罗莉中,王春元.金鱼(Carassius auratus L.)同工酶的发生遗传学研究Ⅱ.金鱼胚胎发育不同时期乳酸脱氢酶同工酶分析[J].遗传学报,1984,11(6):457-493.
[25]朱蓝菲,陈湘粦,王祖熊. 20种鲤科鱼类同工酶的表型分析及有关进化问题的探讨[J].水产学报,1983,7(2):145-152.
[26]祁得林.青海湖裸鲤和鲤鱼组织乳酸脱氢酶同工酶比较研究[J].青海大学学报:自然科学版,2003,21(6):1-3.
[27]魏乐.青海湖裸鲤、鲤鱼的血红蛋白的电泳研究[J].青海师范大学学报:自然科学版,2000(2):39-43.
[28]秦桂香,朱海梅,李谕,等.青海湖裸鲤过氧化氢酶活性及电泳分析[J].黑龙江畜牧兽医报,2014,7:181-182
[29] Chen Y F,He D K,Chen Y Y. Electrophoretic analysis of isozymes and discussion about species differentiation in three species of Genus gymnocypris[J]. Zoological Research,2001,22(1):9-19.
[30]胡思玉,彭垠,赵海涛,等.四川裂腹鱼同工酶研究[J].湖北农业科学,2015,54(18):4540-4543.