白细胞介素-21及白细胞介素-17和IgA肾病进展的相关性研究
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摘要
目的探讨免疫因子白细胞介素-21(IL-21)及白细胞介素-17(IL-17)和IgA肾病(IgAN)进展的相关性。方法纳入经肾穿刺活检明确诊断为IgAN的患者共34例,收集IgAN患者清晨空腹静脉血清及临床资料,用ELISA方法检测IgAN患者IL-21、IL-17的表达,比较血清IL-21、IL-17的表达与肌酐(Scr)、尿酸(UA)、评估肾小球滤过率(eGFR)、24 h尿蛋白定量之间的相关性。结果 IgAN患者血清中IL-21的表达与尿酸和24 h尿蛋白呈正相关性(r=0.341、0.449,P<0.05)。IL-21的表达与Scr和eGFR之间无相关性(P>0.05)。IgAN患者血清中IL-17的表达与Scr、eGFR、24 h尿蛋白呈正相关性(r=0.347、0.347、0.363,P<0.05)。结论 IL-21和IL-17与监测IgAN的临床指标存在相关性,可作为IgAN疾病进展的预测因子。
Objective To investigate the correlation between the progress of IgA nephropathy(IgAN) and immunokines interleukin-21(IL-21),interleukin-17(IL-17).Methods 34 patients with IgAN diagnosed by renal biopsy were enrolled. The fasting venous serum were collected in the morning and the clinical data of IgAN patients were collected,including age,sex,medication history,24-hour urinary protein quantification,serum uric acid,creatinine and evaluation of glomerular filtration rate(eGFR). The expression of IL-21 and IL-17 in IgAN patients was detected by ELISA. The correlation between the expression of IL-21 and IL-17 in serum and creatinine(SCr),uric acid(UA),glomerular filtration rate(eGFR) and 24-hour urinary protein quantification was compared.Results The expression of IL-21 in serum of IgAN patients was positively correlated with uric acid and 24-hour urinary protein(r=0.341,0.449,P<0.05). There was no correlation between IL-21 and creatinine and eGFR(P>0.05). The expression of IL-17 in serum of IgAN patients was positively correlated with creatinine,eGFR and 24-hour urinary protein(r=0.347,0.347,0.363,P<0.05). There was no correlation between the expression of IL-17 and uric acid(P>0.05).Conclusion IL-21 and IL-17 are correlated with clinical indicators of IgAN monitoring,and may be used as predictors of IgAN disease progress.
Objective To investigate the correlation between the progress of IgA nephropathy(IgAN) and immunokines interleukin-21(IL-21),interleukin-17(IL-17).Methods 34 patients with IgAN diagnosed by renal biopsy were enrolled. The fasting venous serum were collected in the morning and the clinical data of IgAN patients were collected,including age,sex,medication history,24-hour urinary protein quantification,serum uric acid,creatinine and evaluation of glomerular filtration rate(eGFR). The expression of IL-21 and IL-17 in IgAN patients was detected by ELISA. The correlation between the expression of IL-21 and IL-17 in serum and creatinine(SCr),uric acid(UA),glomerular filtration rate(eGFR) and 24-hour urinary protein quantification was compared.Results The expression of IL-21 in serum of IgAN patients was positively correlated with uric acid and 24-hour urinary protein(r=0.341,0.449,P<0.05). There was no correlation between IL-21 and creatinine and eGFR(P>0.05). The expression of IL-17 in serum of IgAN patients was positively correlated with creatinine,eGFR and 24-hour urinary protein(r=0.347,0.347,0.363,P<0.05). There was no correlation between the expression of IL-17 and uric acid(P>0.05).Conclusion IL-21 and IL-17 are correlated with clinical indicators of IgAN monitoring,and may be used as predictors of IgAN disease progress.
引文
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[15] 林芙君,刘秀君.白细胞介素-17在IgA肾病肾小管间质的表达及意义[J].临床肾脏病杂志,2010,10(10):23.
[16] 刘利,杨满,樊均明.Th17细胞在IgA肾病发病机制中的作用[J].中国中西医结合肾病杂志,2012,13(5):462-464.
[2] Shin DH,Lim BJ,HanI M,et al.Glomerular IgG deposition predicts renal outcome in patients with IgA nephropathy[J].Modern Pathology,2016,29(7):743-752.
[3] Davis MR,Zhu Ziwen,Hansen DM,et al.The role of IL-21 in immunity and cancer[J].Cancer Letters,2015,358(2):107-114.
[4] Monteleone G,Pallone F,Macdonald T,et al.Interleukin-2l(IL-21)-mediated pathways in T cell-mediated disease[J].Cytokine & Growth Factor Reviews,2009,20(2):185-191.
[5] Yi JS,Cox MA,Zajac AJ.Interleukin-21:a multifunctional regulator of immunity to infections[J].Microbes and Infection,2010,12(14/15):1111-1119.
[6] Chol YS,Yang JA,Cpotty S.Dyanmic regulation of Bcl6 in follicularhelper CD4 T(Tfh)cells[J].Current Opinion in Immunology,2013,25(3):366-372.
[7] Deenick EK,Tangye SG.Autoimmunity:IL-21:a new player in T h17-cell differentiation[J].Immunology and Cell Biology,2007,85(7):503-505.
[8] Vergano L,Loiacono E,Albera R,et al.Can tonsillectomy modifythe innate and adaptive immunity pathways involved in IgA nephropathy[J].Journal of Nephrology,2015,28(1):51-58.
[9] Kiryluk K,Novak J.The genetics and immunobiology of IgA nephropathy[J].The Journal of Clinical Investigation,2014,124(6):2325-2332.
[10] 王金泉.IgA肾病的遗传易感性和黏膜免疫应答异常[J].医学研究生学报,2016,29(2):120-125.
[11] Coppo R.The intestine-renal connection in IgA nephropathy[J].Nephrology Dialysis Transplantation,2015,30(3):360-366.
[12] Vieira SM,Pagovich OE,Kriegel MD.Microbiota and autoimmune diseases[J].Lupus,2014,23(6):518-526.
[13] 范燕琴,丁国华,陈星华,等.IgA肾病的遗传学和免疫生物学发病机制的研究进展[J].医学研究杂志,2016,45(3):10-13.
[14] Ying S,Zhihong LIU.Increased frequencies of memory and activated B cells andfollicular helper T cells are positively associated with high levels of activation induced cytidine deaminase in patients with immunoglobulin A nephropathy[J].Molecular Medicine Reports,2015,2:5531-5537.
[15] 林芙君,刘秀君.白细胞介素-17在IgA肾病肾小管间质的表达及意义[J].临床肾脏病杂志,2010,10(10):23.
[16] 刘利,杨满,樊均明.Th17细胞在IgA肾病发病机制中的作用[J].中国中西医结合肾病杂志,2012,13(5):462-464.