体外受精分娩儿童的神经行为发育指标研究
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摘要
收集体外受精(in vitro fertilization, IVF)与自然受孕分娩儿童脐血和外周血,提取血清,采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay, ELISA)检测血清脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)水平,并进行自闭症儿童行为量表(autism behavior checklist, ABC)评分,彩色多普勒超声对妊娠11周及21周的胎盘厚度进行测量,了解体外受精及自然受孕儿童神经行为发育的差异。结果显示,体外受精分娩儿童血清BDNF水平低于自然受孕儿童(P<0.01),ABC量表评分高于自然受孕儿童(P<0.05),孕21周胎盘厚度增厚(P<0.05)。这提示体外受精可能会影响胎盘发育,并导致儿童神经行为的改变。
The umbilical cord and peripheral blood of naturally conceived and in vitro fertilization(IVF)children were collected, then serum was extracted. All children were detected the serum brain-derived neurotrophic factor(BDNF) levels with the enzyme-linked immunosorbent assay(ELISA).The neurobehaviour scores were evaluated with the autism behavior checklist(ABC). Color Doppler Ultrasonography was used to measure the placenta thickness at 11 and 21 weeks of pregnancy. To understand the difference of neurobehavioral development between in vitro fertilization and natural conception. The results showed that serum BDNF level of in vitro fertilization children was lower than that of natural conception children(P<0.01),ABC scale score was higher than that of natural conception children(P<0.05), and placental thickness increased at 21 weeks of gestation(P<0.05). This suggests that in vitro fertilization may affect placental development and lead to changes in neural behavior in children.
The umbilical cord and peripheral blood of naturally conceived and in vitro fertilization(IVF)children were collected, then serum was extracted. All children were detected the serum brain-derived neurotrophic factor(BDNF) levels with the enzyme-linked immunosorbent assay(ELISA).The neurobehaviour scores were evaluated with the autism behavior checklist(ABC). Color Doppler Ultrasonography was used to measure the placenta thickness at 11 and 21 weeks of pregnancy. To understand the difference of neurobehavioral development between in vitro fertilization and natural conception. The results showed that serum BDNF level of in vitro fertilization children was lower than that of natural conception children(P<0.01),ABC scale score was higher than that of natural conception children(P<0.05), and placental thickness increased at 21 weeks of gestation(P<0.05). This suggests that in vitro fertilization may affect placental development and lead to changes in neural behavior in children.
引文
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[11]YEUNG E H,SUNDARAM R,BELL E M,et al.Examining infertility treatment and early childhood development in the upstate KIDS study[J].JAMA Pediatr,2016,170(3):251-258.
[12]BRENDGEN M,GIRARD A,VITARO F,et al.Geneenvironment correlation linking aggression and peervictimization:do classroom behavioral norms matter?[J].JAbnorm Child Psychol,2015,43(1):19-31.
[13]MAINIGI M,ROSENZWEIG J M,LEI J,et al.Periimplantation hormonal milieu:elucidating mechanisms of adverse neurodevelopmental outcomes[J].Reprod Sci,2016,23(6):785-794.
[14]SAHAY A S,SUNDRANI D P,JOSHI S R.Neurotrophins:role in placental growth and development[J].Vitam Horm,2017,104:243-261.
[15]YI K L,ZHOU X,SHI D S,et al.The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle[J].Animal,2008,2(12):1786-1794.
[2]PANJA D,BRAMHAM C R.BDNF mechanisms in late LTPformation:A synthesis and breakdown[J].Neuropharmacology,2014,76:664-676.
[3]MAUSSION G,MOALIC J M,SIMONNEAU M,et al.Increased expression of BDNF mRNA in the frontal cortex of autistic patients[J].Behav Brain Res,2018,359(1):903-909.
[4]MCCARTHY D M,BROWN A N,BHIDE P G.Regulation of bdnF expression by cocaine[J].Yale J Biol Med,2012,85(4):437-446.
[5]JIANG H,CHEN S,LI C,et al.The serum protein levels of the t PA-BDNF pathway are implicated in depression and antidepressant treatment[J].Transl Psychiatry,2017,7(4):e1079.
[6]BORBA E M,DUARTE J A,BRISTOT G,et al.Brain-derived neurotrophic factor serum levels and hippocampal volume in mild cognitive impairment and dementia due to alzheimer disease[J].Dement Geriatr Cogn Dis Extra,2016,6(3):559-567.
[7]RAMER I,KRUCZEK A,DOULAVERIS G,et al.Reduced circulating concentration of brain-derived neurotrophic factor is associated with peri-and Post-implantation failure following In vitro fertilization-embryo transfer[J].Am J Reprod Immunol,2016,75(1):36-41.
[8]CHURCHILL S J,WANG E T,AKHLAGHPOUR M,et al.Mode of conception does not appear to affect placental volume in the first trimester[J].Fertil Steril,2017,107(6):1341-1347.
[9]SUNDHEIMER L W,CHAN J L,BUTTLE R,et al.Mode of conception does not affect fetal or placental growth parameters or ratios in early gestation or at delivery[J].J Assist Reprod Genet,2018,35(6):1039-1046.
[10]RAMER I,KANNINEN T T,SISTI G,et al.The serum brainderived neurotrophic factor concentration prior to initiation of an in vitro fertilization cycle predicts outcome[J].J Reprod Immunol,2016,116:46-49.
[11]YEUNG E H,SUNDARAM R,BELL E M,et al.Examining infertility treatment and early childhood development in the upstate KIDS study[J].JAMA Pediatr,2016,170(3):251-258.
[12]BRENDGEN M,GIRARD A,VITARO F,et al.Geneenvironment correlation linking aggression and peervictimization:do classroom behavioral norms matter?[J].JAbnorm Child Psychol,2015,43(1):19-31.
[13]MAINIGI M,ROSENZWEIG J M,LEI J,et al.Periimplantation hormonal milieu:elucidating mechanisms of adverse neurodevelopmental outcomes[J].Reprod Sci,2016,23(6):785-794.
[14]SAHAY A S,SUNDRANI D P,JOSHI S R.Neurotrophins:role in placental growth and development[J].Vitam Horm,2017,104:243-261.
[15]YI K L,ZHOU X,SHI D S,et al.The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle[J].Animal,2008,2(12):1786-1794.