太子参芜菁花叶病毒时空动态定性初步检测
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摘要
为明确柘荣县太子参携带芜菁花叶病毒情况,采用RT-PCR法对不同太子参植株进行芜菁花叶病毒检测。结果表明:同株太子参有无病斑的叶片、根部、茎部都可检测到病毒;未表现病毒症状与表现病毒症状太子参植株的9个部位均可检测到病毒,未表现病毒症状的太子参植株中叶脉及茎下部病毒含量相对较高;不同海拔地区种植的太子参植株10个部位均可检测到病毒,花部位的病毒含量相对较少;对不同生长期太子参定株叶片进行病毒检测发现生长末期部分植株未检测到病毒,除此之外所取样品均携带芜菁花叶病毒。
In order to clarify the situation of turnip mosaic virus carried by radix pseudostellariae in Zherong County, RT-PCR was used to detect turnip mosaic virus in different plants of radix pseudostellariae. The results showed that viruses in could be detected in leaves, roots and stems of the same plant with or without disease spots. Viruses Radix pseudostellariaecould be detected in 9 parts of plants with and without viral symptoms, while the content of viruses in middle veins and lower stems of plants without viral symptoms was relatively higher. Viruses in Radix pseudostellariae could be detected in 10 parts of plants at different altitudes, but the virus content in flower parts was relatively lower. Virus detection on the leaves of fixed plants of radix pseudostellariae at different growth stages showed that no virus was detected in some plants at the end of growth stage. In addition, all the samples carried turnip mosaic virus.
In order to clarify the situation of turnip mosaic virus carried by radix pseudostellariae in Zherong County, RT-PCR was used to detect turnip mosaic virus in different plants of radix pseudostellariae. The results showed that viruses in could be detected in leaves, roots and stems of the same plant with or without disease spots. Viruses Radix pseudostellariaecould be detected in 9 parts of plants with and without viral symptoms, while the content of viruses in middle veins and lower stems of plants without viral symptoms was relatively higher. Viruses in Radix pseudostellariae could be detected in 10 parts of plants at different altitudes, but the virus content in flower parts was relatively lower. Virus detection on the leaves of fixed plants of radix pseudostellariae at different growth stages showed that no virus was detected in some plants at the end of growth stage. In addition, all the samples carried turnip mosaic virus.
引文
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[4]黄颖桢,陈菁瑛,朱景花,等.太子参中芜菁花叶病毒的RT-PCR检测[J].福建农业学报,2016,31(6):616-619.
[5]简熠,周倩,王锡锋,等.白背飞虱体内南方水稻黑条矮缩病毒定量检测体系建立[J].基因组学与应用生物学,2016(35):366-372.
[2]宋荣浩,濮祖芹.太子参病毒病病原鉴定[J].上海农业学报,1991,7(2):80-85.
[3]吴朝峰,林彦铨.药用植物太子参的研究进展[J].福建农林大学学报(自然科学版),2004,34(4):426-430.
[4]黄颖桢,陈菁瑛,朱景花,等.太子参中芜菁花叶病毒的RT-PCR检测[J].福建农业学报,2016,31(6):616-619.
[5]简熠,周倩,王锡锋,等.白背飞虱体内南方水稻黑条矮缩病毒定量检测体系建立[J].基因组学与应用生物学,2016(35):366-372.