低温胁迫下PEG引发对珠芽蓼种子萌发的影响
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摘要
本试验以冬虫夏草寄主蝠蛾幼虫喜食植物珠芽蓼种子为研究对象,分析低温吸胀冷害对珠芽蓼种子萌发的影响,探讨PEG引发处理对预防珠芽蓼种子萌发期吸胀冷害的作用。结果表明低温吸胀处理后珠芽蓼种子的发芽势、发芽率、发芽指数和活力指数分别降低了51. 3%,38. 5%,53. 0%和57. 6%,显著低于对照(P <0. 05)。随着引发时间的延长,珠芽蓼种子的发芽势、发芽率、发芽指数和活力指数均呈现先上升后下降的趋势,且在引发时间为6h～12h之间达到最高值,显著高于对照和其它处理(P <0. 05)。当引发时间为12h时,相对于对照处理,珠芽蓼种子的发芽势、发芽率、发芽指数和活力指数分别提高了16. 4%,9. 1%,18. 3%和29. 1%,差异显著(P <0. 05)。当引发浓度为-0. 6MPa时,珠芽蓼种子经12h和24h的PEG引发处理后发芽势、发芽率、发芽指数和活力指数均达到最高,分别提高了12. 7%,11. 4%,21. 7%和36. 4%,与对照相比差异显著(P <0. 05)。统计分析表明,低温吸胀胁迫处理下珠芽蓼种子的发芽势(F=4. 828,P <0. 001)、发芽率(F=4. 437,P <0. 001)、发芽指数(F=4. 435,P <0. 001)和活力指数(F=3. 868,P=0. 001)明显受PEG引发时间和浓度交互作用的影响。
In this study,the Polygonum viviparum seed were used as experimental materials,which is a favorite food for the host moth larva of Cordyceps sinensis. The aim is to analyze the effects of imbibitions on the germination of Polygonum viviparum seed under low temperature and to investigate the role of PEG on imbibitions of the germination of Polygonum viviparum under low temperature. The results showed that the seed germination potential,germination percentage,germination index and vigor index of Polygonum viviparum decreased by 51. 3%,38. 5%,53. 0% and57. 6%,respectively,significantly( P < 0. 05) lower than the control. With the extended seed priming time,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed increased first and then decreased,and the maximum of all indices at the treatments of 6h and 12 h,significantly( P < 0. 05)higher than the control and other treatments. Compared to the control,when the time of seed priming was at 12 h,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed were significantly( P < 0. 05) increased by 16. 4%,9. 1%,18. 3% and 29. 1%,respectively. Compared to the control,when the concentration of PEG primed was-0. 6 MPa,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed reached the maximum at 12 h and 24 h,they were significantly( P <0. 05) increased by 12. 7%,11. 4%,21. 7% and 36. 4%,respectively. The statistical analysis showed that the seed germination potential( F = 4. 828,P < 0. 001),germination percentage( F = 4. 437,P < 0. 001),germination index( F = 4. 435,P < 0. 001) and vigor index( F = 3. 868,P = 0. 001) of Polygonum viviparum were significantly affected by the interaction of PEG priming imbibitions time and its concentration.
In this study,the Polygonum viviparum seed were used as experimental materials,which is a favorite food for the host moth larva of Cordyceps sinensis. The aim is to analyze the effects of imbibitions on the germination of Polygonum viviparum seed under low temperature and to investigate the role of PEG on imbibitions of the germination of Polygonum viviparum under low temperature. The results showed that the seed germination potential,germination percentage,germination index and vigor index of Polygonum viviparum decreased by 51. 3%,38. 5%,53. 0% and57. 6%,respectively,significantly( P < 0. 05) lower than the control. With the extended seed priming time,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed increased first and then decreased,and the maximum of all indices at the treatments of 6h and 12 h,significantly( P < 0. 05)higher than the control and other treatments. Compared to the control,when the time of seed priming was at 12 h,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed were significantly( P < 0. 05) increased by 16. 4%,9. 1%,18. 3% and 29. 1%,respectively. Compared to the control,when the concentration of PEG primed was-0. 6 MPa,the germination potential,germination percentage,germination index and vigor index of Polygonum viviparum seed reached the maximum at 12 h and 24 h,they were significantly( P <0. 05) increased by 12. 7%,11. 4%,21. 7% and 36. 4%,respectively. The statistical analysis showed that the seed germination potential( F = 4. 828,P < 0. 001),germination percentage( F = 4. 437,P < 0. 001),germination index( F = 4. 435,P < 0. 001) and vigor index( F = 3. 868,P = 0. 001) of Polygonum viviparum were significantly affected by the interaction of PEG priming imbibitions time and its concentration.
引文
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[7]宋建清,叶萌,张议方,等.三种虫草蝙蝠蛾幼虫野生食材的营养成分分析[J].北方园艺,2011(9):157-159.
[8]鲁增辉,伍晓丽,刘飞.冬虫夏草寄主幼虫饲料营养成分比较分析[J].西南农业学报,2013,26(5):2048-2051.
[9]郑光华,顾增辉,徐本美.大豆种子萌发过程中冷害问题的研究[J].中国农业科学,1981,14(2):65-72.
[10]郑光华,张庆昌.聚乙二醇(PEG)渗透调控法处理种子能提高活力、增强抗逆能力[J].种子,1985,2:55-56.
[11]郑光华,张庆昌,燕义唐,等.PEG引发种子应用于大豆抗寒早播的效果[J].种子,1987,1:12-15.
[12]燕义唐.聚乙二醇(PEG)引发预防大豆种子吸胀冷害的效果[J].植物生理学报,1987(4):26-29.
[13]燕义唐,梁峥,郑光华,等.PEG引发提高大豆种子抗吸胀冷害的作用机理---引发对敏感大豆种子氧化磷酸化的效应[J].科学通报,1988,33(6):465-465.
[14]万春光.不同引发处理对水稻苗期耐冷性及老化水稻种子萌发的影响[D].黑龙江大学,2011.
[15]张言芳.水稻幼苗耐温度逆境种子引发剂的筛选与生理作用研究[D].华中农业大学,2012.
[16]禹晓梅,李唯奇.大豆种子吸胀冷害抗性与其质体膜脂不饱和度正相关[J].植物分类与资源学报,2014,36(2):187-196.
[17]Heydecker W,Higgins J,GulliverRL.Accelerated Germinationby Osmotic Seed Treatment[J].Nature,1973,246(5427):42-44.
[18]段里成,张坤,章起明,等.低温胁迫对直播早稻出苗及幼苗生长的影响[J].广东农业科学,2018,3:1-6
[19]郑光华,梁峥.种子吸胀冷害和渗透调控的研究[J].中国科学院院刊,2001,16(3):182-187.
[20]杨云珠,陈文涛,郭金铨.PVA和PEG预处理对大豆种子在低温吸胀过程中胚根线粒体发育和超微结构的影响[J].Journal of Integrative Plant Biology,1992,6:30-34+85-86.
[21]王彦荣.种子引发的研究现状[J].草业学报,2004,13(4):7-12.
[22]陈子敬,于茜,丁云玉,等.种子引发对芹菜种子发芽、幼苗生长及产量的影响[J].种子,2016,35(4):26-29.
[23]王彦荣,张建全,刘慧霞,等.PEG引发紫花苜蓿和沙打旺种子的生理生态效应[J].生态学报,2004,24(3):402-408.
[24]卫亚红,Dorna H,Tylkowska K.化学和生物制剂处理对洋葱种子萌发的影响(英文)[J].西北农林科技大学学报(自然科学版),2003,31(5):51-56.
[2]中国科学院中国植物志编辑委员会,中国植物志(第二十五卷第一分册)[M].北京:科学出版社,1998.
[3]中国科学院植物研究所.中国高等植物志图鉴(第一卷)[M].北京:科学出版社,1972.
[4]中国科学院西北高原生物研究所.青海经济植物志[M].西宁:青海人民出版社,1987.
[5]杜品.青藏高原甘南藏药植物志[M].兰州:甘肃科学技术出版社,2006.
[6]王俊丽,公维镇,肖璇,等.藏药珠芽蓼的研究与应用[J].中央民族大学学报(自然科学版),2010,19(1):28-33.
[7]宋建清,叶萌,张议方,等.三种虫草蝙蝠蛾幼虫野生食材的营养成分分析[J].北方园艺,2011(9):157-159.
[8]鲁增辉,伍晓丽,刘飞.冬虫夏草寄主幼虫饲料营养成分比较分析[J].西南农业学报,2013,26(5):2048-2051.
[9]郑光华,顾增辉,徐本美.大豆种子萌发过程中冷害问题的研究[J].中国农业科学,1981,14(2):65-72.
[10]郑光华,张庆昌.聚乙二醇(PEG)渗透调控法处理种子能提高活力、增强抗逆能力[J].种子,1985,2:55-56.
[11]郑光华,张庆昌,燕义唐,等.PEG引发种子应用于大豆抗寒早播的效果[J].种子,1987,1:12-15.
[12]燕义唐.聚乙二醇(PEG)引发预防大豆种子吸胀冷害的效果[J].植物生理学报,1987(4):26-29.
[13]燕义唐,梁峥,郑光华,等.PEG引发提高大豆种子抗吸胀冷害的作用机理---引发对敏感大豆种子氧化磷酸化的效应[J].科学通报,1988,33(6):465-465.
[14]万春光.不同引发处理对水稻苗期耐冷性及老化水稻种子萌发的影响[D].黑龙江大学,2011.
[15]张言芳.水稻幼苗耐温度逆境种子引发剂的筛选与生理作用研究[D].华中农业大学,2012.
[16]禹晓梅,李唯奇.大豆种子吸胀冷害抗性与其质体膜脂不饱和度正相关[J].植物分类与资源学报,2014,36(2):187-196.
[17]Heydecker W,Higgins J,GulliverRL.Accelerated Germinationby Osmotic Seed Treatment[J].Nature,1973,246(5427):42-44.
[18]段里成,张坤,章起明,等.低温胁迫对直播早稻出苗及幼苗生长的影响[J].广东农业科学,2018,3:1-6
[19]郑光华,梁峥.种子吸胀冷害和渗透调控的研究[J].中国科学院院刊,2001,16(3):182-187.
[20]杨云珠,陈文涛,郭金铨.PVA和PEG预处理对大豆种子在低温吸胀过程中胚根线粒体发育和超微结构的影响[J].Journal of Integrative Plant Biology,1992,6:30-34+85-86.
[21]王彦荣.种子引发的研究现状[J].草业学报,2004,13(4):7-12.
[22]陈子敬,于茜,丁云玉,等.种子引发对芹菜种子发芽、幼苗生长及产量的影响[J].种子,2016,35(4):26-29.
[23]王彦荣,张建全,刘慧霞,等.PEG引发紫花苜蓿和沙打旺种子的生理生态效应[J].生态学报,2004,24(3):402-408.
[24]卫亚红,Dorna H,Tylkowska K.化学和生物制剂处理对洋葱种子萌发的影响(英文)[J].西北农林科技大学学报(自然科学版),2003,31(5):51-56.