矾根叶片离体快繁及工厂化生产技术研究
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摘要
本研究以矾根的幼嫩叶片为外植体,对其进行组培快繁和规模化生产研究,建立了矾根叶片离体快繁体系,并进行工厂化生产技术研究。试验结果表明:最佳芽诱导培养基为MS添加BAA 2.0 mg/L+NAA0.10 mg/L;最佳增殖壮苗培养基为MS添加BAA 1.0 mg/L+NAA 0.10 mg/L;最佳生根培养基为1/2 MS添加NAA 0.10 mg/L,所获得的组培苗驯化移栽一个月后成活率可达90%以上。
In this study, the young leaves of Heuchera micrantha were used as explants. The tissue culture, rapid propagation and large scale production of it were carried out. Then, the in vitro rapid propagation system was established and the technique for industrial production was investigated. The results showed that MS +6-BA 2.0 mg/L+NAA 0.10 mg/L was the optimal bud induction medium, MS+6-BA 1.0 mg/L+NAA 0.10 mg/L was the optimal medium for proliferation of seedling, and 1/2 MS +NAA 0.10 mg/L was the optimal root induction medium. The survival rate of plantlets obtained after domestication and transplanting for one month was over 90%.
In this study, the young leaves of Heuchera micrantha were used as explants. The tissue culture, rapid propagation and large scale production of it were carried out. Then, the in vitro rapid propagation system was established and the technique for industrial production was investigated. The results showed that MS +6-BA 2.0 mg/L+NAA 0.10 mg/L was the optimal bud induction medium, MS+6-BA 1.0 mg/L+NAA 0.10 mg/L was the optimal medium for proliferation of seedling, and 1/2 MS +NAA 0.10 mg/L was the optimal root induction medium. The survival rate of plantlets obtained after domestication and transplanting for one month was over 90%.
引文
Cheng H.,T ang Y.,Shi Y.H.,and Wu J.,2011,Tissue culture and rapid propagation of Heuchera micrantha,Shangha Nongye Xuebao(Acta Agriculturae Shanghai),27(4):80-82(陈宏,唐莹,施月欢,吴杰,2011,矾根的组织培养与快速繁殖,上海农业学报,27(4):80-82)
Gao Y.,Song L.,Ye K.,Cai X.F.,and Feng S.C.,2015,Tissue culture and rapid propagation of Heuchera micrantha Obsidian,Zhejiang Nongye Kexue(Zhejiang Agricultural Sciences),56(6):895-898(高燕,宋垚,叶康,蔡新风,封树成,2015,矾根欧布西迪昂组培快繁技术,浙江农业科学56(6):895-898)
Liang F.,Bai S.Y.,and Ma Y.,2009,Study on evaluation and cultivation of five perennial flowers,Caoyuan Yu Caoping(Grassland and Turf),(6):43-46(梁芳,白淑媛,马燕,20095种宿根花卉品种性生长习评价及栽培研究,草原与草坪,(6):43-46)
Sun G.F.,Zhang J.Z.,and Wu D.Q.,2007,Tissue culture and rapid propagation of Heuche ra micrantha Hort.Price of Silver,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):500(孙国锋,张金政,吴东启,2007,矾根杂种“银王子”的组织培养和快速繁殖,植物生理学通讯,43(3):500)
Wang J.,Liu L.G.,Zuo L.J.,and Tang C.L.,2012,Study on tissue culture and rapid propagation of Heuchera villosa,Beifang Yuanyi(Northern Horticulture),(23):116-118(王晶,刘立功,左丽娟,唐存莲,2012,柔毛矾根组培快繁技术研究,北方园艺,(23):116-118)
Zhang D.X.,Zhou Z.C.,Bu Y.L.,Zhang J.,Zhang X.W.,and Zhang C.B.,2011,Research progress on effect of drought stress on the physiological property and microstructure in grapevine,Beifang Yuanyi(Northern Horticulture),(6):209-213(张东旭,周增产,卜云龙,张洁,张晓文,张成波,2011,植物组织培养技术应用研究进展,北方园艺,(6):209-213)
Zhang Z.H.,Cao H.M.,and Zhou S.J.,2011,Tissue culture and rapid propagation of Heuchera micrantha purple palace,Jiangsu Nongye Kexue(Jiangsu Agricultural Sciences),39(5):69-71(章志红,曹慧敏,周士景,2011,美洲矾根品种紫宫殿组织培养与离体快繁研究,江苏农业科学,39(5):69-71)
Gao Y.,Song L.,Ye K.,Cai X.F.,and Feng S.C.,2015,Tissue culture and rapid propagation of Heuchera micrantha Obsidian,Zhejiang Nongye Kexue(Zhejiang Agricultural Sciences),56(6):895-898(高燕,宋垚,叶康,蔡新风,封树成,2015,矾根欧布西迪昂组培快繁技术,浙江农业科学56(6):895-898)
Liang F.,Bai S.Y.,and Ma Y.,2009,Study on evaluation and cultivation of five perennial flowers,Caoyuan Yu Caoping(Grassland and Turf),(6):43-46(梁芳,白淑媛,马燕,20095种宿根花卉品种性生长习评价及栽培研究,草原与草坪,(6):43-46)
Sun G.F.,Zhang J.Z.,and Wu D.Q.,2007,Tissue culture and rapid propagation of Heuche ra micrantha Hort.Price of Silver,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):500(孙国锋,张金政,吴东启,2007,矾根杂种“银王子”的组织培养和快速繁殖,植物生理学通讯,43(3):500)
Wang J.,Liu L.G.,Zuo L.J.,and Tang C.L.,2012,Study on tissue culture and rapid propagation of Heuchera villosa,Beifang Yuanyi(Northern Horticulture),(23):116-118(王晶,刘立功,左丽娟,唐存莲,2012,柔毛矾根组培快繁技术研究,北方园艺,(23):116-118)
Zhang D.X.,Zhou Z.C.,Bu Y.L.,Zhang J.,Zhang X.W.,and Zhang C.B.,2011,Research progress on effect of drought stress on the physiological property and microstructure in grapevine,Beifang Yuanyi(Northern Horticulture),(6):209-213(张东旭,周增产,卜云龙,张洁,张晓文,张成波,2011,植物组织培养技术应用研究进展,北方园艺,(6):209-213)
Zhang Z.H.,Cao H.M.,and Zhou S.J.,2011,Tissue culture and rapid propagation of Heuchera micrantha purple palace,Jiangsu Nongye Kexue(Jiangsu Agricultural Sciences),39(5):69-71(章志红,曹慧敏,周士景,2011,美洲矾根品种紫宫殿组织培养与离体快繁研究,江苏农业科学,39(5):69-71)