地方特色金针菜品种大乌嘴提纯复壮技术研究
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摘要
以大乌嘴金针菜的幼嫩茎尖作为外植体进行离体培养,成功建立大乌嘴组培快繁体系。结果表明:不同激素种类及质量浓度对大乌嘴初代培养、继代培养和生根培养影响差异显著。诱导愈伤组织的最适激素浓度为6-BA 3 mg/L和NAA 0.4 mg/L;诱导不定芽的最适激素浓度为6-BA 3 mg/L和NAA 0.2 mg/L;诱导生根的最适激素浓度为NAA0.2 mg/L或IBA0.2 mg/L。该研究为金针菜品种大乌嘴大规模生产提供科学依据。
The shoot tip of daylily Da Wu Zui was used to establish techniques and a system for its tissue culture. Different hormone types and concentrations were examined in the study and it was found that these had significant effects on primary culture,starting culture,subculture and rooting culture of Da Wu Zui. The most suitable hormone concentration for callus Induction was MS supplemented with 3 mg/L 6-BA and 0.4 mg/L NAA. The most suitable hormone concentration for induction of adventitious buds was MS supplemented with 3 mg/L 6-BA and 0.2 mg/L NAA. The most suitable hormone concentration for root formation was 1/2 MS with 0.2 mg/L NAA or 0.2 mg/L IBA. This study provides a scientific basis for the large-scale production of daylily Da Wu Zui.
The shoot tip of daylily Da Wu Zui was used to establish techniques and a system for its tissue culture. Different hormone types and concentrations were examined in the study and it was found that these had significant effects on primary culture,starting culture,subculture and rooting culture of Da Wu Zui. The most suitable hormone concentration for callus Induction was MS supplemented with 3 mg/L 6-BA and 0.4 mg/L NAA. The most suitable hormone concentration for induction of adventitious buds was MS supplemented with 3 mg/L 6-BA and 0.2 mg/L NAA. The most suitable hormone concentration for root formation was 1/2 MS with 0.2 mg/L NAA or 0.2 mg/L IBA. This study provides a scientific basis for the large-scale production of daylily Da Wu Zui.
引文
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[13]王玲,祝朋芳,毛洪玉.不同培养基及不同贮藏条件对金娃娃萱草花粉生命力的影响[J].西北林学院学报,2009,24(3):95-97.
[14]杨丽莉,张晓,晋凡生,等.激素对萱草组织培养参数的影响[J].山西农业科学,2012,40(8):815-818.
[15]赵玉芬,储博彦,尹新彦,等.白砂糖对大花萱草红运试管苗生根的影响[J].林业科技开发,2013,27(4):30-32.
[16]吕秀立,施季森,吕光明,等.双玫瑰萱草的离体培养和快速繁殖[J].杂草科学,2009(1):31-33.
[17]高凤,王雪,张婷婷,等.萱草引进新品种ForgottenDreams的组织培养技术[J].江苏农业科学,2012,40(8):60-62.
[18]王蓉,杨彬,李秀霞.红宝石萱草的组织培养快繁技术研究[J].北方园艺,2009(9):173-175.
[19]赵玉芬,储博彦,尹新彦,等.应用正交实验优化大花萱草试管苗生根的研究[J].北方园艺,2013(6):100-102.
[2]杨丽莉,王德平,张晓,等.以子房为外植体的金娃娃萱草组织培养技术的研究[J].中国农学通报,2012,28(25):184-190.
[3]毕晓颖,王宁.萱草花茎离体培养与快速繁殖[J].东北林业大学学报,2012,40(11):56-60.
[4]李秀华,杜贞,武银玉,等.大花萱草组培快繁体系的研究[J].植物研究,2009,29(6):757-762.
[5]卢宝伟,安凤霞,郑宝仁,等.忘萱草新品种红宝石花托的离体培养与规模化生产[J].植物生理学通讯,2010,46(3):257-258.
[6]杨丽莉,张晓,张彦琴.大花萱草东方不败的组织培养技术研究[J].山西农业科学,2011,39(7):629-632.
[7]赵玉芬,储博彦,尹新彦,等.大花萱草杂交种子试管内萌发及快繁技术研究[J].种子,2011,39(7):629-632.
[8]何月秋,祝志勇.大花萱草新品种御衣黄组织培养及植株再生[J].江苏林业科技,2011,38(3):12-16.
[9]史淑一,彭广霖,丛惠芳,等.大花萱草组培苗的炼苗技术[J].北方园艺,2011(10):111-113.
[10]杨丽莉,张晓,杨睿,等.大花萱草莎蔓的组织培养技术研究[J].北方园艺,2012(19):134-137.
[11]黄花花,曹冬梅,魏爱丽,等.大花萱草黄绣客愈伤组织玻璃化的初步研究[J].山西农业科学,2011,39(3):213-216.
[12]赵玉芬,储博彦,尹新彦,等.大花萱草工厂化快繁技术研究[J].北方园艺,2011(2):152-155.
[13]王玲,祝朋芳,毛洪玉.不同培养基及不同贮藏条件对金娃娃萱草花粉生命力的影响[J].西北林学院学报,2009,24(3):95-97.
[14]杨丽莉,张晓,晋凡生,等.激素对萱草组织培养参数的影响[J].山西农业科学,2012,40(8):815-818.
[15]赵玉芬,储博彦,尹新彦,等.白砂糖对大花萱草红运试管苗生根的影响[J].林业科技开发,2013,27(4):30-32.
[16]吕秀立,施季森,吕光明,等.双玫瑰萱草的离体培养和快速繁殖[J].杂草科学,2009(1):31-33.
[17]高凤,王雪,张婷婷,等.萱草引进新品种ForgottenDreams的组织培养技术[J].江苏农业科学,2012,40(8):60-62.
[18]王蓉,杨彬,李秀霞.红宝石萱草的组织培养快繁技术研究[J].北方园艺,2009(9):173-175.
[19]赵玉芬,储博彦,尹新彦,等.应用正交实验优化大花萱草试管苗生根的研究[J].北方园艺,2013(6):100-102.