快速诱导人骨髓间充质干细胞成脂培养基的研发
摘要
<正>间充质干细胞(MSCs)是在正常组织中发现的一种多能祖细胞[1],因其具有独特的自我更新能力和分化成脂肪细胞、骨细胞、心肌细胞、神经元细胞等组织特异性细胞的潜能[2-4],广泛用于再生医学研究,MSCs在组织损伤修复[4]、缺血性心肌病[5]、急性心肌梗死[6]、肝纤维化[7]等疾病治疗中发挥着重要作用。目前,面临的主要挑战是实现MSCs的大量来源及保证安全性,MSCs定义的主要特征是它的自我更新潜力、表面标记的表达以及多谱系分化能力,因此,向成脂肪细胞分化是鉴别MSCs不可少的部分。此外,MSCs的脂肪分化在再生医学研究中具有重要意义。从临床的角度来看,外科医生面临着患者软组织吸收重建的挑战。例如,烧伤患者经常遭受
引文
[1] Lacerda L,Debeb BG,Smith D,et al. Mesenchymal stem cells mediate the clinical phenotype of inflammatory breast cancer in a preclinical model[J]. Breast Cancer Res,2015,17(1):42.
[2] Pittenger MF,Mackay AM,Beck SC,et al. Multilineage potential of adult human mesenchymal stem cells[J]. Science,1999,284(5411):143-147.
[3] Cao M,Mao J,Duan X,et al. In vivo tracking of the tropism of mesenchymal stem cells to malignant gliomas using reporter gene-based MR imaging[J]. Intern J Cancer,2018,142(5):1033-1046.
[4] Samsonraj RM,Raghunath M,Nurcombe V,et al. Concise review:multifaceted characterization of human mesenchymal stem cells for use in regenerative medicine[J]. Stem Cells Transl Med,2017,6(12):2173-2185.
[5] Golpanian S,Wolf A,Hatzistergos KE,et al. Rebuilding the damaged heart:mesenchymal stem cells, cell-based therapy,and engineered heart tissue[J]. Physiol Rev,2016,96(3):1127-1168.
[6] Bian S,Zhang L,Duan L,et al. Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model[J]. J Molec Med,2014,92(4):387-397.
[7] Liu WH,Song FQ,Ren LN,et al. The multiple functional roles of mesenchymal stem cells in participating in treating liver diseases[J]. J Cell Mol Med,2015,19(3):511-520.
[8] Scott MA,Nguyen VT,Levi B,et al. Current methods of adipogenic differentiation of mesenchymal stem cells[J]. Stem Cells Develop,2011,20(10):1793-1804.
[9] Khanmohammadi M,Khanjani S,Edalatkhah H,et al. Modified protocol for improvement of differentiation potential of menstrual blood-derived stem cells into adipogenic lineage[J]. Cell Prolif,2014,47(6):615-623.
[10] Jing Z,Qiong Z,Yonggang W,et al. Rat bone marrow mesenchymal stem cells improve regeneration of thin endometrium in rat[J]. Fertil Steril,2014,101(2):587-594.
[11] Park JH,Park W,Cho S,et al. Nanofunctionalized stent-mediated local heat treatment for the suppression of stent-induced tissue hyperplasia[J]. ACS Appl Mater Interfaces,2018,10:29357-29366.
[12] Hammarlund E,Thomas A,Amanna IJ,et al. Plasma cell survival in the absence of B cell memory[J]. Nature Commun,2017,8(1):1781.
[13] Rzonca SO,Suva LJ,Gaddy D,et al. Bone is a target for the antidiabetic compound rosiglitazone[J]. Endocrinology,2004,145(1):401-406.
[14] Ali AA,Weinstein RS,Stewart SA,et al. Rosiglitazone causes bone loss in mice by suppressing osteoblast differentiation and bone formation[J]. Endocrinology,2005,146(3):1226-1235.
[15] Chen Q,Shou P,Zheng C,et al. Fate decision of mesenchymal stem cells:Adipocytes or osteoblasts[J]. Cell Death Differ,2016,23(7):1128-1139.
[16] Song HF,He S,Li SH,et al. Aged human multipotent mesenchymal stromal cells can be rejuvenated by neuron-derived neurotrophic factor and improve heart function after injury[J]. Jacc Basic Transl Sci,2017,2(6):702-716.
[2] Pittenger MF,Mackay AM,Beck SC,et al. Multilineage potential of adult human mesenchymal stem cells[J]. Science,1999,284(5411):143-147.
[3] Cao M,Mao J,Duan X,et al. In vivo tracking of the tropism of mesenchymal stem cells to malignant gliomas using reporter gene-based MR imaging[J]. Intern J Cancer,2018,142(5):1033-1046.
[4] Samsonraj RM,Raghunath M,Nurcombe V,et al. Concise review:multifaceted characterization of human mesenchymal stem cells for use in regenerative medicine[J]. Stem Cells Transl Med,2017,6(12):2173-2185.
[5] Golpanian S,Wolf A,Hatzistergos KE,et al. Rebuilding the damaged heart:mesenchymal stem cells, cell-based therapy,and engineered heart tissue[J]. Physiol Rev,2016,96(3):1127-1168.
[6] Bian S,Zhang L,Duan L,et al. Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model[J]. J Molec Med,2014,92(4):387-397.
[7] Liu WH,Song FQ,Ren LN,et al. The multiple functional roles of mesenchymal stem cells in participating in treating liver diseases[J]. J Cell Mol Med,2015,19(3):511-520.
[8] Scott MA,Nguyen VT,Levi B,et al. Current methods of adipogenic differentiation of mesenchymal stem cells[J]. Stem Cells Develop,2011,20(10):1793-1804.
[9] Khanmohammadi M,Khanjani S,Edalatkhah H,et al. Modified protocol for improvement of differentiation potential of menstrual blood-derived stem cells into adipogenic lineage[J]. Cell Prolif,2014,47(6):615-623.
[10] Jing Z,Qiong Z,Yonggang W,et al. Rat bone marrow mesenchymal stem cells improve regeneration of thin endometrium in rat[J]. Fertil Steril,2014,101(2):587-594.
[11] Park JH,Park W,Cho S,et al. Nanofunctionalized stent-mediated local heat treatment for the suppression of stent-induced tissue hyperplasia[J]. ACS Appl Mater Interfaces,2018,10:29357-29366.
[12] Hammarlund E,Thomas A,Amanna IJ,et al. Plasma cell survival in the absence of B cell memory[J]. Nature Commun,2017,8(1):1781.
[13] Rzonca SO,Suva LJ,Gaddy D,et al. Bone is a target for the antidiabetic compound rosiglitazone[J]. Endocrinology,2004,145(1):401-406.
[14] Ali AA,Weinstein RS,Stewart SA,et al. Rosiglitazone causes bone loss in mice by suppressing osteoblast differentiation and bone formation[J]. Endocrinology,2005,146(3):1226-1235.
[15] Chen Q,Shou P,Zheng C,et al. Fate decision of mesenchymal stem cells:Adipocytes or osteoblasts[J]. Cell Death Differ,2016,23(7):1128-1139.
[16] Song HF,He S,Li SH,et al. Aged human multipotent mesenchymal stromal cells can be rejuvenated by neuron-derived neurotrophic factor and improve heart function after injury[J]. Jacc Basic Transl Sci,2017,2(6):702-716.